Q6ZDE3 (ABAH2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Abscisic acid 8'-hydroxylase 2
Short name=ABA 8'-hydroxylase 2
EC=1.14.13.93

Alternative name(s):
Cytochrome P450 707A6
OsABA8ox2

Gene names

Name:	CYP707A6
Synonyms:	ABA8OX2
Ordered Locus Names:	Os08g0472800, LOC_Os08g36860
ORF Names:	OsJ_026541, P0013B04.19

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the oxidative degradation of abscisic acid.
Catalytic activity	+-abscisate + NADPH + O2 = 8'-hydroxyabscisate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Plant hormone degradation; abscisic acid degradation.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Induction	Not induced by ethylene treatment or flooding. Ref.5
Sequence similarities	Belongs to the cytochrome P450 family.
Sequence caution	The sequence EAZ43058.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	abscisic acid catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway release of seed from dormancy Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to red light Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	(+)-abscisic acid 8'-hydroxylase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Abscisic acid 8'-hydroxylase 2		PRO_0000288646
Regions
	Transmembrane	3 – 23	21	Helical; Potential
	Compositional bias	45 – 48	4	Poly-Lys
	Compositional bias	260 – 284	25	Gly-rich
Sites
	Metal binding	441	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	430	1	T → S in AK120757. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q6ZDE3 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C1F583725741091E	FASTA	510	56,556
	10 20 30 40 50 60 MAFLLFFVFV TAAVLCFVVP AFLLLCTSVQ RRRDVGQGGG RDWQKKKKLR LPPGSMGWPY 70 80 90 100 110 120 VGETLQLYSQ DPNVFFASKQ KRYGEIFKTN LLGCPCVMLA SPEAARFVLV SQARLFKPTY 130 140 150 160 170 180 PPSKERMIGP SALFFHQGEY HLRLRRLVQA ALAPDSLRAL VPDVDAAVAA TLAAWSGGHV 190 200 210 220 230 240 ASTFHAMKKL SFDVGVVTIF GGRLGRRHRE ELRTNYSVVE RGYNCFPNRF PGTLYHKAIQ 250 260 270 280 290 300 ARKRLRAILS EIVAERRARG GGGGGGGDDL LGGLMRSRDD GTAGAVALLT DDQIADNVVG 310 320 330 340 350 360 VLFAAQDTTA SVLTWILKYL HDSPKLLEAV KAEQMAIYVA NEGGKRPLTW TQTRSMTLTH 370 380 390 400 410 420 QVILESLRMA SIISFTFREA VADVEYKGFL IPKGWKVMPL FRNIHHNPDY FQDPQKFDPS 430 440 450 460 470 480 RFKVAPRPST FLPFGSGVHA CPGNELAKLE MLVLVHRLVT AYRWEIVGAS DEVEYSPFPV 490 500 510 PRGGLNAKLW KQEAEEDMYM AMGTITAAGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[5]	"Ethylene promotes submergence-induced expression of OsABA8ox1, a gene that encodes ABA 8'-hydroxylase in rice." Saika H., Okamoto M., Miyoshi K., Kushiro T., Shinoda S., Jikumaru Y., Fujimoto M., Arikawa T., Takahashi H., Ando M., Arimura S., Miyao A., Hirochika H., Kamiya Y., Tsutsumi N., Nambara E., Nakazono M. Plant Cell Physiol. 48:287-298(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, LACK OF INDUCTION. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP004457 Genomic DNA. Translation: BAD09367.1. AP008214 Genomic DNA. Translation: BAF23935.1. CM000145 Genomic DNA. Translation: EAZ43058.1. Sequence problems. AK120757 mRNA. No translation available.
RefSeq	NP_001062021.1. NM_001068556.1.
UniGene	Os.50329.
3D structure databases
ProteinModelPortal	Q6ZDE3.
ModBase	Search...
Protein-protein interaction databases
STRING	39947.LOC_Os08g36860.1.
Proteomic databases
PaxDb	Q6ZDE3.
PRIDE	Q6ZDE3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os08g36860.1; LOC_Os08g36860.1; LOC_Os08g36860.
GeneID	4345810.
KEGG	dosa:Os08t0472800-01. osa:4345810.
Organism-specific databases
Gramene	Q6ZDE3.
Phylogenomic databases
eggNOG	COG2124.
HOGENOM	HOG000237614.
KO	K09843.
OMA	SALCHIE.
ProtClustDB	CLSN2697395.
Enzyme and pathway databases
UniPathway	UPA00093.
Gene expression databases
ArrayExpress	Q6ZDE3.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ABAH2_ORYSJ

Accession

Primary (citable) accession number: Q6ZDE3
Secondary accession number(s): A3BU19

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program