Reviewed, UniProtKB/Swiss-Prot Q6ZDE3 (ABAH2_ORYSJ)

Names and origin

Protein names

Recommended name:
    Abscisic acid 8'-hydroxylase 2
      Short name=ABA 8'-hydroxylase 2
    EC=1.14.13.93
Alternative name(s):
    OsABA8ox2
    Cytochrome P450 707A6

Gene names

Name:	CYP707A6
Synonyms:	ABA8OX2
Ordered Locus Names:	Os08g0472800, LOC_Os08g36860
ORF Names:	P0013B04.19, OsJ_026541

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the oxidative degradation of abscisic acid.
Catalytic activity	(+)-abscisate + NADPH + O(2) = 8'-hydroxyabscisate + NADP(+) + H(2)O.
Cofactor	Heme group By similarity.
Pathway	Plant hormone degradation; abscisic acid degradation.
Subcellular location	Membrane; Single-pass membrane proteinPotential.
Induction	Not induced by ethylene treatment or flooding.
Sequence similarities	Belongs to the cytochrome P450 family.
Sequence caution	The sequence EAZ43058.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	(+)-abscisic acid 8'-hydroxylase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Abscisic acid 8'-hydroxylase 2		PRO_0000288646
Regions
	Transmembrane	3 – 23	21	Potential
	Compositional bias	45 – 48	4	Poly-Lys
	Compositional bias	260 – 284	25	Gly-rich
Sites
	Metal binding	441	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	430	1	T → S in AK120757. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q6ZDE3-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C1F583725741091E	FASTA	510	56,556
	10 20 30 40 50 60 MAFLLFFVFV TAAVLCFVVP AFLLLCTSVQ RRRDVGQGGG RDWQKKKKLR LPPGSMGWPY 70 80 90 100 110 120 VGETLQLYSQ DPNVFFASKQ KRYGEIFKTN LLGCPCVMLA SPEAARFVLV SQARLFKPTY 130 140 150 160 170 180 PPSKERMIGP SALFFHQGEY HLRLRRLVQA ALAPDSLRAL VPDVDAAVAA TLAAWSGGHV 190 200 210 220 230 240 ASTFHAMKKL SFDVGVVTIF GGRLGRRHRE ELRTNYSVVE RGYNCFPNRF PGTLYHKAIQ 250 260 270 280 290 300 ARKRLRAILS EIVAERRARG GGGGGGGDDL LGGLMRSRDD GTAGAVALLT DDQIADNVVG 310 320 330 340 350 360 VLFAAQDTTA SVLTWILKYL HDSPKLLEAV KAEQMAIYVA NEGGKRPLTW TQTRSMTLTH 370 380 390 400 410 420 QVILESLRMA SIISFTFREA VADVEYKGFL IPKGWKVMPL FRNIHHNPDY FQDPQKFDPS 430 440 450 460 470 480 RFKVAPRPST FLPFGSGVHA CPGNELAKLE MLVLVHRLVT AYRWEIVGAS DEVEYSPFPV 490 500 510 PRGGLNAKLW KQEAEEDMYM AMGTITAAGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[5]	"Ethylene promotes submergence-induced expression of OsABA8ox1, a gene that encodes ABA 8'-hydroxylase in rice." Saika H., Okamoto M., Miyoshi K., Kushiro T., Shinoda S., Jikumaru Y., Fujimoto M., Arikawa T., Takahashi H., Ando M., Arimura S., Miyao A., Hirochika H., Kamiya Y., Tsutsumi N., Nambara E., Nakazono M. Plant Cell Physiol. 48:287-298(2007) [PubMed: 17205969] [Abstract] Cited for: IDENTIFICATION, LACK OF INDUCTION. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP004457 Genomic DNA. Translation: BAD09367.1. AP008214 Genomic DNA. Translation: BAF23935.1. CM000145 Genomic DNA. Translation: EAZ43058.1. Sequence problems. AK120757 mRNA. No translation available.
RefSeq	NP_001062021.1.
UniGene	Os.50329
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4345810.
KEGG	osa:4345810.
Organism-specific databases
Gramene	Q6ZDE3.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ABAH2_ORYSJ

Accession

Primary (citable) accession number: Q6ZDE3
Secondary accession number(s): A3BU19

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)