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Protein

Flavone 3'-O-methyltransferase 1

Gene

ROMT-9

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Methylates OH residues of flavonoid compounds.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + 3'-hydroxyflavone = S-adenosyl-L-homocysteine + 3'-methoxyflavone.

Kineticsi

Measured at pH 7.5 and 37 degrees Celsius for all experiments.

  1. KM=61.8 µM for quercetin1 Publication
  2. KM=61.9 µM for luteolin1 Publication
  3. KM=62 µM for eriodictyol1 Publication
  1. Vmax=1250 pmol/sec/mg enzyme with quercetin as substrate1 Publication
  2. Vmax=833 pmol/sec/mg enzyme with luteolin as substrate1 Publication
  3. Vmax=625 pmol/sec/mg enzyme with eriodictyol as substrate1 Publication

Pathwayi: quercetin degradation

This protein is involved in the pathway quercetin degradation, which is part of Flavonoid metabolism.
View all proteins of this organism that are known to be involved in the pathway quercetin degradation and in Flavonoid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei212 – 2121S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei235 – 2351S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei255 – 2551S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei256 – 2561S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
Binding sitei270 – 2701S-adenosyl-L-methioninePROSITE-ProRule annotation
Active sitei274 – 2741Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  • caffeate O-methyltransferase activity Source: CACAO
  • luteolin O-methyltransferase activity Source: UniProtKB
  • O-methyltransferase activity Source: GO_Central

GO - Biological processi

  • aromatic compound biosynthetic process Source: GO_Central
  • methylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-OSA-209931. Serotonin and melatonin biosynthesis.
UniPathwayiUPA00724.

Names & Taxonomyi

Protein namesi
Recommended name:
Flavone 3'-O-methyltransferase 1 (EC:2.1.1.42)
Short name:
OsOMT1
Alternative name(s):
Quercetin 3'-O-methyltransferase 1
Gene namesi
Name:ROMT-9
Synonyms:COMT
Ordered Locus Names:Os08g0157500, LOC_Os08g06100
ORF Names:OsJ_26105, P0438H08.29
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 8

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 221 PublicationPRO_0000248969
Chaini3 – 368366Flavone 3'-O-methyltransferase 1PRO_0000233699Add
BLAST

Proteomic databases

PaxDbiQ6ZD89.
PRIDEiQ6ZD89.

Expressioni

Tissue specificityi

Expressed in roots and stems, and at lower levels in leaves.1 Publication

Gene expression databases

ExpressionAtlasiQ6ZD89. baseline and differential.
GenevisibleiQ6ZD89. OS.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi39947.LOC_Os08g06100.1.

Structurei

3D structure databases

ProteinModelPortaliQ6ZD89.
SMRiQ6ZD89. Positions 12-368.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. COMT subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3178. Eukaryota.
ENOG410XS7T. LUCA.
HOGENOMiHOG000238276.
InParanoidiQ6ZD89.
OMAiPPHGKVI.
OrthoDBiEOG09360E51.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR016461. O-MeTrfase_COMT.
IPR001077. O_MeTrfase_2.
IPR012967. Plant_MeTrfase_dimerisation.
IPR029063. SAM-dependent_MTases.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF08100. Dimerisation. 1 hit.
PF00891. Methyltransf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF005739. O-mtase. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51683. SAM_OMT_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6ZD89-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSTAADMAA AADEEACMYA LQLASSSILP MTLKNAIELG LLETLQSAAV
60 70 80 90 100
AGGGGKAALL TPAEVADKLP SKANPAAADM VDRMLRLLAS YNVVRCEMEE
110 120 130 140 150
GADGKLSRRY AAAPVCKWLT PNEDGVSMAA LALMNQDKVL MESWYYLKDA
160 170 180 190 200
VLDGGIPFNK AYGMTAFEYH GTDARFNRVF NEGMKNHSVI ITKKLLDLYT
210 220 230 240 250
GFDAASTVVD VGGGVGATVA AVVSRHPHIR GINYDLPHVI SEAPPFPGVE
260 270 280 290 300
HVGGDMFASV PRGGDAILMK WILHDWSDEH CARLLKNCYD ALPEHGKVVV
310 320 330 340 350
VECVLPESSD ATAREQGVFH VDMIMLAHNP GGKERYEREF RELARAAGFT
360
GFKATYIYAN AWAIEFTK
Length:368
Mass (Da):39,749
Last modified:July 5, 2004 - v1
Checksum:i2491B4FA7447558E
GO

Sequence cautioni

The sequence EAZ41571 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 541G → R in EAZ41571 (PubMed:15685292).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ288259 mRNA. Translation: ABB90678.1.
DQ530257 mRNA. Translation: ABF72191.1.
AP004460 Genomic DNA. Translation: BAC99512.1.
AP008214 Genomic DNA. Translation: BAF22945.1.
AP014964 Genomic DNA. Translation: BAT03903.1.
CM000145 Genomic DNA. Translation: EAZ41571.1. Different initiation.
AK064768 mRNA. Translation: BAG89194.1.
AB122056 mRNA. Translation: BAD14923.1.
RefSeqiXP_015650053.1. XM_015794567.1.
UniGeneiOs.11202.

Genome annotation databases

EnsemblPlantsiOS08T0157500-01; OS08T0157500-01; OS08G0157500.
GeneIDi4344702.
GrameneiOS08T0157500-01; OS08T0157500-01; OS08G0157500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ288259 mRNA. Translation: ABB90678.1.
DQ530257 mRNA. Translation: ABF72191.1.
AP004460 Genomic DNA. Translation: BAC99512.1.
AP008214 Genomic DNA. Translation: BAF22945.1.
AP014964 Genomic DNA. Translation: BAT03903.1.
CM000145 Genomic DNA. Translation: EAZ41571.1. Different initiation.
AK064768 mRNA. Translation: BAG89194.1.
AB122056 mRNA. Translation: BAD14923.1.
RefSeqiXP_015650053.1. XM_015794567.1.
UniGeneiOs.11202.

3D structure databases

ProteinModelPortaliQ6ZD89.
SMRiQ6ZD89. Positions 12-368.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os08g06100.1.

Proteomic databases

PaxDbiQ6ZD89.
PRIDEiQ6ZD89.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS08T0157500-01; OS08T0157500-01; OS08G0157500.
GeneIDi4344702.
GrameneiOS08T0157500-01; OS08T0157500-01; OS08G0157500.

Phylogenomic databases

eggNOGiKOG3178. Eukaryota.
ENOG410XS7T. LUCA.
HOGENOMiHOG000238276.
InParanoidiQ6ZD89.
OMAiPPHGKVI.
OrthoDBiEOG09360E51.

Enzyme and pathway databases

UniPathwayiUPA00724.
ReactomeiR-OSA-209931. Serotonin and melatonin biosynthesis.

Gene expression databases

ExpressionAtlasiQ6ZD89. baseline and differential.
GenevisibleiQ6ZD89. OS.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR016461. O-MeTrfase_COMT.
IPR001077. O_MeTrfase_2.
IPR012967. Plant_MeTrfase_dimerisation.
IPR029063. SAM-dependent_MTases.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF08100. Dimerisation. 1 hit.
PF00891. Methyltransf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF005739. O-mtase. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51683. SAM_OMT_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOMT1_ORYSJ
AccessioniPrimary (citable) accession number: Q6ZD89
Secondary accession number(s): A0A0P0XBV5
, A3BPT2, Q19BJ6, Q75W57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Was initially thought to constitute the naringenin 7-O-methyltransferase (NOMT), a methyltransferase involved in the biosynthesis of the sakuranetin, an inducible defense mechanism of O.sativa against pathogen attack (PubMed:10814825). However, it was later shown that it is not the case (PubMed:22493492).2 Publications

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.