Q6ZD89 (OMT1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Quercetin 3-O-methyltransferase 1
EC=2.1.1.76

Alternative name(s):
Flavonol 3-O-methyltransferase 1

Gene names

Name:	ROMT-9
Synonyms:	COMT
Ordered Locus Names:	Os08g0157500, LOC_Os08g06100
ORF Names:	P0438H08.29

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Methylates OH residues of flavonoid compounds. Substrate preference is quercetin > luteolin >> myricetin >>> eriodictyol. Ref.1
Catalytic activity	S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone.
Pathway	Flavonoid metabolism; quercetin degradation.
Subunit structure	Monomer By similarity.
Tissue specificity	Expressed in roots and stems, and at lower levels in leaves. Ref.1
Sequence similarities	Belongs to the methyltransferase superfamily. Type 2 family. COMT subfamily.
Biophysicochemical properties	Kinetic parameters: Measured at pH 7.5 and 37 degrees Celsius for all experiments. KM=61.8 µM for quercetin Ref.1 KM=61.9 µM for luteolin KM=62 µM for eriodictyol Vmax=1250 pmol/sec/mg enzyme with quercetin as substrate Vmax=833 pmol/sec/mg enzyme with luteolin as substrate Vmax=625 pmol/sec/mg enzyme with eriodictyol as substrate

Ontologies

Keywords
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	protein dimerization activity Inferred from electronic annotation. Source: InterPro quercetin 3-O-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 2	2			PRO_0000248969
	Chain	3 – 368	366	Quercetin 3-O-methyltransferase 1		PRO_0000233699
Sites
	Active site	274	1	Proton acceptor By similarity
	Binding site	212	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	235	1	S-adenosyl-L-methionine By similarity
	Binding site	255	1	S-adenosyl-L-methionine By similarity
	Binding site	256	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	270	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6ZD89 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 2491B4FA7447558E	FASTA	368	39,749
	10 20 30 40 50 60 MGSTAADMAA AADEEACMYA LQLASSSILP MTLKNAIELG LLETLQSAAV AGGGGKAALL 70 80 90 100 110 120 TPAEVADKLP SKANPAAADM VDRMLRLLAS YNVVRCEMEE GADGKLSRRY AAAPVCKWLT 130 140 150 160 170 180 PNEDGVSMAA LALMNQDKVL MESWYYLKDA VLDGGIPFNK AYGMTAFEYH GTDARFNRVF 190 200 210 220 230 240 NEGMKNHSVI ITKKLLDLYT GFDAASTVVD VGGGVGATVA AVVSRHPHIR GINYDLPHVI 250 260 270 280 290 300 SEAPPFPGVE HVGGDMFASV PRGGDAILMK WILHDWSDEH CARLLKNCYD ALPEHGKVVV 310 320 330 340 350 360 VECVLPESSD ATAREQGVFH VDMIMLAHNP GGKERYEREF RELARAAGFT GFKATYIYAN AWAIEFTK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Flavonoid 3'-O-methyltransferase from rice: cDNA cloning, characterization and functional expression." Kim B.-G., Lee Y., Hur H.-G., Lim Y., Ahn J.-H. Phytochemistry 67:387-394(2006) [PubMed: 16412485] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Proteomic analysis of rice leaf, stem and root tissues during growth course." Nozu Y., Tsugita A., Kamijo K. Proteomics 6:3665-3670(2006) [PubMed: 16758443] [Abstract] Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 3-9, MASS SPECTROMETRY. Strain: cv. Nipponbare.
[4]	"The cDNA encoding caffeic acid o-methyl transferase expressed in rice stem." Mase K., Nishikubo N., Satou K., Nakano Y., Kajita S., Katayama Y. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 101-368. Strain: cv. Fujiminori.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ288259 mRNA. Translation: ABB90678.1. AP004460 Genomic DNA. Translation: BAC99512.1. AB122056 mRNA. Translation: BAD14923.1.
RefSeq	NP_001061031.1. NM_001067566.1.
UniGene	Os.11202.
3D structure databases
ProteinModelPortal	Q6ZD89.
SMR	Q6ZD89. Positions 12-368.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6ZD89.
Proteomic databases
PRIDE	Q6ZD89.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os08g06100.1; LOC_Os08g06100.1; LOC_Os08g06100.
GeneID	4344702.
KEGG	osa:4344702.
Organism-specific databases
Gramene	Q6ZD89.
Phylogenomic databases
GeneTree	EPGT00070000028016.
OMA	MESWYYL.
PhylomeDB	Q6ZD89.
ProtClustDB	CLSN2697139.
Family and domain databases
InterPro	IPR016461. O-MeTrfase_COMT_euk. IPR001077. O_MeTrfase_2. IPR012967. Plant_MeTrfase_dimerisation. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K05279.
Pfam	PF08100. Dimerisation. 1 hit. PF00891. Methyltransf_2. 1 hit. [Graphical view]
PIRSF	PIRSF005739. O-mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

OMT1_ORYSJ

Accession

Primary (citable) accession number: Q6ZD89
Secondary accession number(s): Q75W57

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program