Q6ZAC1 (4CL5_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 4-coumarate--CoA ligase 5
Short name=4CL 5
Short name=Os4CL5
EC=6.2.1.12

Alternative name(s):
4-coumaroyl-CoA synthase 5

Gene names

Name:	4CL5
Ordered Locus Names:	Os08g0448000, LOC_Os08g34790
ORF Names:	OsJ_026405, P0409A07.17, P0429B05.38

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	539 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.
Pathway	Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step 1/2.
Domain	Both substrate-binding domains (SBD1 and SBD2) are involved in the substrate recognition, and are sufficient to confer the substrate specificity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Phenylpropanoid metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phenylpropanoid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	4-coumarate-CoA ligase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 539	539	Probable 4-coumarate--CoA ligase 5		PRO_0000351626
Regions
	Nucleotide binding	185 – 193	9	ATP By similarity
	Nucleotide binding	328 – 333	6	ATP By similarity
	Region	258 – 327	70	SBD1
	Region	328 – 395	68	SBD2
Sites
	Binding site	416	1	ATP By similarity
	Binding site	431	1	ATP By similarity
	Binding site	522	1	ATP By similarity
Experimental info
	Sequence conflict	38	1	D → G in AK120964. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q6ZAC1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 08C1BAEF972189FD	FASTA	539	57,045
	10 20 30 40 50 60 MGSLPEQFVF RSRLPDIAIP DHLPLHDYVF ERLADRRDRA CLIDGATGET LSFGDVDALS 70 80 90 100 110 120 RRVAAGLSSI GVCHGSTVML LLPNSVEFAV AFLASSRLGA VTTTANPLHT PPEIAKQVAA 130 140 150 160 170 180 SGATVVVTEP AFVAKVSGLA GVTVVATGGG AERCASFAGL AAADGSALPE VAIDVANDAV 190 200 210 220 230 240 ALPYSSGTTG LPKGVMLSHR GLVTSVAQLV DGENPNLHLR EDDVVLCVLP MFHVYSLHSI 250 260 270 280 290 300 LLCGMRAGAA IVVMKRFDTV KMLQLVERHG VTIAPLVPPI VVEMAKSDAL DRHDLSSIRM 310 320 330 340 350 360 VISGAAPMGK ELQDIVHAKL PNAVLGQGYG MTEAGPVLSM CMAFAKEPTP VKSGACGTVV 370 380 390 400 410 420 RNAELKIVDP DTGLSLPRNQ PGEICIRGKQ IMKGYLNNPE ATEKTIDKDG WLHTGDIGFV 430 440 450 460 470 480 DDDDEIFIVD RLKELIKYKG FQVAPAELEA MLIAHAAVAD AAVVPMKDDS CGEIPVAFVV 490 500 510 520 530 ARDGSGITDD EIKQYVAKQV VFYKRLHKIF FVDAIPKAPS GKILRKDLRA KLAAGIPAC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[5]	"Genome-wide analysis of a land plant-specific acyl:coenzyme A synthetase (ACS) gene family in Arabidopsis, poplar, rice and Physcomitrella." de Azevedo Souza C., Barbazuk B., Ralph S.G., Bohlmann J., Hamberger B., Douglas C.J. New Phytol. 179:987-1003(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP004558 Genomic DNA. Translation: BAD09442.1. AP004665 Genomic DNA. Translation: BAD09825.1. AP008214 Genomic DNA. Translation: BAF23849.1. CM000145 Genomic DNA. Translation: EAZ42922.1. AK120964 mRNA. No translation available.
RefSeq	NP_001061935.1. NM_001068470.1.
UniGene	Os.19375.
3D structure databases
HSSP	HSSP built from PDB template 1LCI based on UniProtKB P08659.
ProteinModelPortal	Q6ZAC1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os08g34790.1; LOC_Os08g34790.1; LOC_Os08g34790.
GeneID	4345717.
KEGG	osa:4345717.
Organism-specific databases
Gramene	Q6ZAC1.
Phylogenomic databases
eggNOG	COG0318.
HOGENOM	HOG000230009.
KO	K01904.
OMA	MPVQQAV.
ProtClustDB	PLN02246.
Enzyme and pathway databases
UniPathway	UPA00372; UER00547.
Gene expression databases
ArrayExpress	Q6ZAC1.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. IPR025110. DUF4009. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. PF13193. DUF4009. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

4CL5_ORYSJ

Accession

Primary (citable) accession number: Q6ZAC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program