Reviewed, UniProtKB/Swiss-Prot Q6ZAC1 (4CL5_ORYSJ)

Names and origin

Protein names

Recommended name:
    Probable 4-coumarate--CoA ligase 5
      Short name=4CL 5
      Short name=Os4CL5
    EC=6.2.1.12
Alternative name(s):
    4-coumaroyl-CoA synthase 5

Gene names

Name:	4CL5
Ordered Locus Names:	Os08g0448000, LOC_Os08g34790
ORF Names:	P0409A07.17, P0429B05.38, OsJ_026405

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	539 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.
Pathway	Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from 4-coumaric acid: step 1/2.
Domain	Both substrate-binding domains (SBD1 and SBD2) are involved in the substrate recognition, and are sufficient to confer the substrate specificity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Phenylpropanoid metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	phenylpropanoid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-coumarate-CoA ligase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 539	539	Probable 4-coumarate--CoA ligase 5		PRO_0000351626
Regions
	Nucleotide binding	185 – 193	9	ATP By similarity
	Nucleotide binding	328 – 333	6	ATP By similarity
	Region	258 – 327	70	SBD1
	Region	328 – 395	68	SBD2
Sites
	Binding site	416	1	ATP By similarity
	Binding site	431	1	ATP By similarity
	Binding site	522	1	ATP By similarity
Experimental info
	Sequence conflict	38	1	D → G in AK120964. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q6ZAC1-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 08C1BAEF972189FD	FASTA	539	57,045
	10 20 30 40 50 60 MGSLPEQFVF RSRLPDIAIP DHLPLHDYVF ERLADRRDRA CLIDGATGET LSFGDVDALS 70 80 90 100 110 120 RRVAAGLSSI GVCHGSTVML LLPNSVEFAV AFLASSRLGA VTTTANPLHT PPEIAKQVAA 130 140 150 160 170 180 SGATVVVTEP AFVAKVSGLA GVTVVATGGG AERCASFAGL AAADGSALPE VAIDVANDAV 190 200 210 220 230 240 ALPYSSGTTG LPKGVMLSHR GLVTSVAQLV DGENPNLHLR EDDVVLCVLP MFHVYSLHSI 250 260 270 280 290 300 LLCGMRAGAA IVVMKRFDTV KMLQLVERHG VTIAPLVPPI VVEMAKSDAL DRHDLSSIRM 310 320 330 340 350 360 VISGAAPMGK ELQDIVHAKL PNAVLGQGYG MTEAGPVLSM CMAFAKEPTP VKSGACGTVV 370 380 390 400 410 420 RNAELKIVDP DTGLSLPRNQ PGEICIRGKQ IMKGYLNNPE ATEKTIDKDG WLHTGDIGFV 430 440 450 460 470 480 DDDDEIFIVD RLKELIKYKG FQVAPAELEA MLIAHAAVAD AAVVPMKDDS CGEIPVAFVV 490 500 510 520 530 ARDGSGITDD EIKQYVAKQV VFYKRLHKIF FVDAIPKAPS GKILRKDLRA KLAAGIPAC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[5]	"Genome-wide analysis of a land plant-specific acyl:coenzyme A synthetase (ACS) gene family in Arabidopsis, poplar, rice and Physcomitrella." de Azevedo Souza C., Barbazuk B., Ralph S.G., Bohlmann J., Hamberger B., Douglas C.J. New Phytol. 179:987-1003(2008) [PubMed: 18627494] [Abstract] Cited for: GENE FAMILY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP004558 Genomic DNA. Translation: BAD09442.1. AP004665 Genomic DNA. Translation: BAD09825.1. AP008214 Genomic DNA. Translation: BAF23849.1. CM000145 Genomic DNA. Translation: EAZ42922.1. AK120964 mRNA. No translation available.
RefSeq	NP_001061935.1.
UniGene	Os.19375
3D structure databases
HSSP	HSSP built from PDB template 1BA3 based on UniProtKB P08659.
ModBase	Search...
Genome annotation databases
GeneID	4345717.
KEGG	osa:4345717.
Organism-specific databases
Gramene	Q6ZAC1.
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

4CL5_ORYSJ

Accession

Primary (citable) accession number: Q6ZAC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	July 5, 2004
Last modified:	March 24, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)