Q6ZA06 (GUN20_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 20
EC=3.2.1.4

Alternative name(s):
Endo-1,4-beta glucanase 20
OsGLU15

Gene names

Name:	GLU15
Ordered Locus Names:	Os08g0387400, LOC_Os08g29770
ORF Names:	P0434E03.25

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	516 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 516	493	Endoglucanase 20		PRO_0000249297
Sites
	Active site	416	1	By similarity
	Active site	468	1	By similarity
	Active site	477	1	By similarity
Amino acid modifications
	Glycosylation	83	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6ZA06 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 1DFC890CB93E2872	FASTA	516	55,111
	10 20 30 40 50 60 MAAGMVATMV LLTCLAAGGL VVGAEEDGGG GGLGRLGSPD YGDALAKAIL FFEGQRSGRL 70 80 90 100 110 120 PANQRATWRG DSALTDGREE NVNLTGGYYD AGDNVKFGYP MAFTVTLLGW SAVEYGAAVA 130 140 150 160 170 180 AAGELGNLRA AIRWGADFLL RAHASPTTLY TQVGDGNADH QCWERPEDMD TPRTLYKITA 190 200 210 220 230 240 DSPGSEAAAE ASAALAAAYV ALKDDGDTAF SSRLLAASRS LFDFANNYRG SFQSSCPFYC 250 260 270 280 290 300 SYSGFQDELL WASAWLFKAT RDAKYLDFLT NNQGSSNPVN EFSWDNKYAG AQMLAAQEYL 310 320 330 340 350 360 GGRTQLARYK DNLDSFVCAL MPNSGNVQIR TTPGGLLFTR DSVNLQYTTT ATLVLSIYSK 370 380 390 400 410 420 VLKSSGSRGV RCSAATFSPN QISSFATSQV DYILGKNPLG MSYMVGFSTK FPRRIHHRGS 430 440 450 460 470 480 SIPSIKVLSR KVTCKEGFSS WLPTSDPNPN IHVGAIVGGP DGNDQFSDNR GDSSHSEPAT 490 500 510 YINAAFVGAC AAAMGQKQVV KLEEPADNLE SMVSTY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP004689 Genomic DNA. Translation: BAD05437.1. AP008214 Genomic DNA. Translation: BAF23627.1. AK071784 mRNA. Translation: BAG92694.1.
RefSeq	NP_001061713.1. NM_001068248.1.
UniGene	Os.53285.
3D structure databases
HSSP	HSSP built from PDB template 1TF4 based on UniProtKB P26221.
ProteinModelPortal	Q6ZA06.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os08g29770.1; LOC_Os08g29770.1; LOC_Os08g29770.
GeneID	4345463.
KEGG	dosa:Os08t0387400-01. osa:4345463.
Organism-specific databases
Gramene	Q6ZA06.
Phylogenomic databases
eggNOG	NOG275252.
HOGENOM	HOG000021033.
OMA	NLTGGYY.
ProtClustDB	PLN02613.
Gene expression databases
ArrayExpress	Q6ZA06.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. [Graphical view]
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. False negative. PS00698. GLYCOSYL_HYDROL_F9_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN20_ORYSJ

Accession

Primary (citable) accession number: Q6ZA06
Secondary accession number(s): Q0J5Y8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program