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Protein

Endoglucanase 20

Gene

GLU15

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei416 – 4161By similarity
Active sitei468 – 4681By similarity
Active sitei477 – 4771By similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
  2. cell wall organization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 20 (EC:3.2.1.4)
Alternative name(s):
Endo-1,4-beta glucanase 20
OsGLU15
Gene namesi
Name:GLU15
Ordered Locus Names:Os08g0387400, LOC_Os08g29770
ORF Names:P0434E03.25
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 8

Organism-specific databases

GrameneiQ6ZA06.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 516493Endoglucanase 20PRO_0000249297Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi83 – 831N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ6ZA06.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG275252.
HOGENOMiHOG000021033.
InParanoidiQ6ZA06.
OMAiPDGNDQF.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6ZA06-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAGMVATMV LLTCLAAGGL VVGAEEDGGG GGLGRLGSPD YGDALAKAIL
60 70 80 90 100
FFEGQRSGRL PANQRATWRG DSALTDGREE NVNLTGGYYD AGDNVKFGYP
110 120 130 140 150
MAFTVTLLGW SAVEYGAAVA AAGELGNLRA AIRWGADFLL RAHASPTTLY
160 170 180 190 200
TQVGDGNADH QCWERPEDMD TPRTLYKITA DSPGSEAAAE ASAALAAAYV
210 220 230 240 250
ALKDDGDTAF SSRLLAASRS LFDFANNYRG SFQSSCPFYC SYSGFQDELL
260 270 280 290 300
WASAWLFKAT RDAKYLDFLT NNQGSSNPVN EFSWDNKYAG AQMLAAQEYL
310 320 330 340 350
GGRTQLARYK DNLDSFVCAL MPNSGNVQIR TTPGGLLFTR DSVNLQYTTT
360 370 380 390 400
ATLVLSIYSK VLKSSGSRGV RCSAATFSPN QISSFATSQV DYILGKNPLG
410 420 430 440 450
MSYMVGFSTK FPRRIHHRGS SIPSIKVLSR KVTCKEGFSS WLPTSDPNPN
460 470 480 490 500
IHVGAIVGGP DGNDQFSDNR GDSSHSEPAT YINAAFVGAC AAAMGQKQVV
510
KLEEPADNLE SMVSTY
Length:516
Mass (Da):55,111
Last modified:July 5, 2004 - v1
Checksum:i1DFC890CB93E2872
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP004689 Genomic DNA. Translation: BAD05437.1.
AP008214 Genomic DNA. Translation: BAF23627.1.
AK071784 mRNA. Translation: BAG92694.1.
RefSeqiNP_001061713.1. NM_001068248.1.
UniGeneiOs.53285.

Genome annotation databases

EnsemblPlantsiOS08T0387400-01; OS08T0387400-01; OS08G0387400.
GeneIDi4345463.
KEGGiosa:4345463.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP004689 Genomic DNA. Translation: BAD05437.1.
AP008214 Genomic DNA. Translation: BAF23627.1.
AK071784 mRNA. Translation: BAG92694.1.
RefSeqiNP_001061713.1. NM_001068248.1.
UniGeneiOs.53285.

3D structure databases

ProteinModelPortaliQ6ZA06.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS08T0387400-01; OS08T0387400-01; OS08G0387400.
GeneIDi4345463.
KEGGiosa:4345463.

Organism-specific databases

GrameneiQ6ZA06.

Phylogenomic databases

eggNOGiNOG275252.
HOGENOMiHOG000021033.
InParanoidiQ6ZA06.
OMAiPDGNDQF.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  3. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiGUN20_ORYSJ
AccessioniPrimary (citable) accession number: Q6ZA06
Secondary accession number(s): Q0J5Y8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: July 5, 2004
Last modified: January 7, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.