Reviewed, UniProtKB/Swiss-Prot Q6Z9U7 (MCES1_ORYSJ)

Names and origin

Protein names

Recommended name:
    mRNA cap guanine-N7 methyltransferase 1
    EC=2.1.1.56
Alternative name(s):
    mRNA (guanine-N(7)-)-methyltransferase 1
    mRNA cap methyltransferase 1

Gene names

Ordered Locus Names:	Os08g0180000, LOC_Os08g08200
ORF Names:	P0455A11.11

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC By similarity.
Catalytic activity	S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. mRNA cap methyltransferase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	mRNA cap guanine-N7 methyltransferase 1		PRO_0000248332
Regions
	Region	70 – 71	2	mRNA cap binding By similarity
Sites
	Binding site	74	1	S-adenosyl-L-methionine By similarity
	Binding site	92	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	95	1	mRNA cap By similarity
	Binding site	101	1	mRNA cap By similarity
	Binding site	114	1	S-adenosyl-L-methionine By similarity
	Binding site	126	1	mRNA cap By similarity
	Binding site	149	1	S-adenosyl-L-methionine By similarity
	Binding site	171	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	175	1	mRNA cap By similarity
	Binding site	263	1	mRNA cap By similarity
	Binding site	332	1	mRNA cap By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6Z9U7-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C18AD9B1B380AE62	FASTA	369	42,040
	10 20 30 40 50 60 MNKRPRDEPS SSFASAPKRQ YGAGGGGYGG HGYSEERSSA RRVADHYSAR SNQTLEEREN 70 80 90 100 110 120 SPIIHLKKLN NWIKSVLIQL YAHPGDCVLD LACGKGGDLI KWDKAKVGYY VGVDIAEGSI 130 140 150 160 170 180 KDCMTRYNGD TDQQRRKKFS FPARLICADC YEARLDEHLY EDAPFDICSC QFALHYSWST 190 200 210 220 230 240 EARARQALAN VSALLRPGGV FIGTMPDANV IIKRLRETDG MEFGNGVYWI SFGEEYAEKK 250 260 270 280 290 300 FPASRPFGIK YKFHLEDAVD CPEWVVPFHL FKLLAEEYDL ELVLTKNFHE FVHEYLQKPE 310 320 330 340 350 360 FAELMRRLGA LGDGRQDQST LSQDEWEVAY LYLAFVLRKR GQPPSQRRAN NANRGKMFLT ENDIDFLGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP004692 Genomic DNA. Translation: BAD17036.1. AP008214 Genomic DNA. Translation: BAF23050.1. AK063927 mRNA. No translation available.
RefSeq	NP_001061136.1.
UniGene	Os.21877
3D structure databases
HSSP	HSSP built from PDB template 1RI5 based on UniProtKB Q8SR66.
SMR	Q6Z9U7. Positions 63-340.
ModBase	Search...
Genome annotation databases
GeneID	4344818.
KEGG	osa:4344818.
Organism-specific databases
Gramene	Q6Z9U7.
Phylogenomic databases
HOGENOM	HBG377892.
PhylomeDB	Q6Z9U7.
Family and domain databases
InterPro	IPR016899. mRNA_G-N7_MeTrfase. IPR004971. Pox_MCEL. [Graphical view]
Pfam	PF03291. Pox_MCEL. 1 hit. [Graphical view]
PIRSF	PIRSF028762. ABD1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCES1_ORYSJ

Accession

Primary (citable) accession number: Q6Z9U7
Secondary accession number(s): Q0J7L5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	July 5, 2004
Last modified:	January 19, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)