Reviewed, UniProtKB/Swiss-Prot Q6Z8L2 (LAC9_ORYSJ)

Names and origin

Protein names

Recommended name:
    Putative laccase-9
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase 9
    Urishiol oxidase 9
    Diphenol oxidase 9

Gene names

Name:	LAC9
Ordered Locus Names:	Os02g0749700, LOC_Os02g51440
ORF Names:	P0431B06.7, OsJ_008109

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	579 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products By similarity.
Catalytic activity	4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O.
Cofactor	Binds 4 copper ions per monomer By similarity.
Subcellular location	Secreted › extracellular space › apoplastPotential.
Sequence similarities	Belongs to the multicopper oxidase family. Contains 3 plastocyanin-like domains.

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Apoplast Secreted
Domain	Repeat Signal
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	copper ion binding Inferred from electronic annotation. Source: InterPro laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 579	552	Putative laccase-9		PRO_0000291894
Regions
	Domain	36 – 152	117	Plastocyanin-like 1
	Domain	162 – 319	158	Plastocyanin-like 2
	Domain	436 – 563	128	Plastocyanin-like 3
Sites
	Metal binding	86	1	Copper 1 By similarity
	Metal binding	88	1	Copper 2 By similarity
	Metal binding	131	1	Copper 2 By similarity
	Metal binding	133	1	Copper 3 By similarity
	Metal binding	480	1	Copper 4 By similarity
	Metal binding	483	1	Copper 1 By similarity
	Metal binding	485	1	Copper 3 By similarity
	Metal binding	542	1	Copper 3 By similarity
	Metal binding	543	1	Copper 4 By similarity
	Metal binding	544	1	Copper 2 By similarity
	Metal binding	548	1	Copper 4 By similarity
	Metal binding	553	1	Copper 4 By similarity
Amino acid modifications
	Glycosylation	41	1	N-linked (GlcNAc...) Potential
	Glycosylation	82	1	N-linked (GlcNAc...) Potential
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	307	1	N-linked (GlcNAc...) Potential
	Glycosylation	405	1	N-linked (GlcNAc...) Potential
	Glycosylation	446	1	N-linked (GlcNAc...) Potential
	Glycosylation	496	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6Z8L2-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 68CE38C42525296C	FASTA	579	64,371
	10 20 30 40 50 60 MGTAKLPALL WLLAGVVLAL AVNPAHGAKT RHYDFFITET NYTRLCHEKS ILTVNGQFPG 70 80 90 100 110 120 PTIYARKGDL VIVNVHNNGN KNITIHWHGV DQPRNPWSDG PEFITQCPIR PGGNFTYQVI 130 140 150 160 170 180 LSEEEGTLWW HAHSDFDRAT VHGAIVIHPK RGTTFPFKKP DKEIPVILGE WWNDDIEHVL 190 200 210 220 230 240 DKAQLLGGDV DPSNANTINA QPGDMFPCSR DDTFKVAVQQ GNTYLLRIIN AGLTNDMFFA 250 260 270 280 290 300 IAGHRLTVVG IDARYTKPLT VDYIMIAPGQ TMDVLLEAKR TLGSNSRYYM AARTFITLPL 310 320 330 340 350 360 DTIPFNNSTA TAIVEYTDSV TARPVGPPEF PVQLPAIKDE NAAMAFVTQL RSLGNQEHPV 370 380 390 400 410 420 HVPTHVDEHM LIDIDINVLP CDPTNMAEKC KEGPQGNRFA ASLNNVSFQS PAIDVLDAYY 430 440 450 460 470 480 YSSGHGVYEE DFPNKPTAFV DPPVNNGSGP LMTKRGTKVK VLEYGTVVEV VFHDLSSENH 490 500 510 520 530 540 PMHLHGFAFY VVGRGNGTFD ESRDPATYNL VDPPFQNTVS VPRSGWAAIR FRADNPGVWF 550 560 570 MHCHFDRHVV WGMDTVFIVK DGKTPQAQML PRPPNMPQC

References

[1]

"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

[2]

"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP004774 Genomic DNA. Translation: BAD15631.1. CM000139 Genomic DNA. Translation: EAZ24626.1.
3D structure databases
HSSP	HSSP built from PDB template 1AOZ based on UniProtKB P37064.
ModBase	Search...
Organism-specific databases
Gramene	Q6Z8L2.
Family and domain databases
InterPro	IPR001117. Cu-oxidase. IPR011706. Cu-oxidase_2. IPR011707. Cu-oxidase_3. IPR002355. Cu_oxidase_Cu_BS. IPR008972. Cupredoxin. IPR017761. Laccase. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 3 hits.
Pfam	PF00394. Cu-oxidase. 1 hit. PF07731. Cu-oxidase_2. 1 hit. PF07732. Cu-oxidase_3. 1 hit. [Graphical view]
PROSITE	PS00079. MULTICOPPER_OXIDASE1. 1 hit. PS00080. MULTICOPPER_OXIDASE2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC9_ORYSJ

Accession

Primary (citable) accession number: Q6Z8L2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)