Q6Z829 (WEE1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Wee1-like protein kinase
EC=2.7.10.2

Gene names

Name:	WEE1
Ordered Locus Names:	Os02g0135300, LOC_Os02g04240
ORF Names:	P0585B01.27

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence BAF07730.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication checkpoint Inferred from electronic annotation. Source: EnsemblPlants/Gramene cell cycle arrest Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein serine/threonine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 520	520	Wee1-like protein kinase		PRO_0000295673
Regions
	Domain	267 – 510	244	Protein kinase
	Nucleotide binding	273 – 281	9	ATP By similarity
Sites
	Active site	387	1	Proton acceptor By similarity
	Metal binding	392	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	404	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	296	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6Z829 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 934F492877B99F9F	FASTA	520	58,391
	10 20 30 40 50 60 MLRTKTPRPR GGKSRRATAA AGKERERERE REREGRSPSG ELSLQLEHVS LFSFLADAPR 70 80 90 100 110 120 EGAAAARTPF TPFEELLEGS CDPDPTPPPP LPPLQPQATP MDADEVVEEK DSGILSQDFF 130 140 150 160 170 180 CTPDYITPDA PQLGSGFDAN KENIPCPNSP EKSVCRSKRY KRDCSPKGLG SNDIFDSQWI 190 200 210 220 230 240 APVQFEGLDD SEEEQLKESS SHKRGSYVSQ SAVALRCRVM PPPCIRNPYL NTDHQIDDNV 250 260 270 280 290 300 FGGRQCKSSG FSPSVDGDGL SRYRTDFHEI EQIGRGNFSV VFKVLKRIDG CLYAVKRSIR 310 320 330 340 350 360 QLHNDRERRQ AVKEVQALAA LGCHENIVGY FTSWFENKQL FIQMELCDRC LSMDRNQPLK 370 380 390 400 410 420 CGEALELLYQ ICKGLDFIHE RGIAHLDVKP DNIYVRNGVY KLGDFGCATL IDRSLAIEDG 430 440 450 460 470 480 DSRYMPPEML NDKYEHLDKV DIFSLGAAIY ELIRGTQLPD SGPQFTSLRE GKIALLPGCP 490 500 510 520 MQFQSLIKSM MDPDPVRRPS AKEVLRHPIF DKLHKAPAKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Genome-wide identification and expression analysis of rice cell cycle genes." Guo J., Song J., Wang F., Zhang X.S. Plant Mol. Biol. 64:349-360(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP004799 Genomic DNA. Translation: BAD10095.1. AP008208 Genomic DNA. Translation: BAF07730.2. Sequence problems.
RefSeq	NP_001045816.2. NM_001052351.2.
UniGene	Os.98767.
3D structure databases
HSSP	HSSP built from PDB template 2A19 based on UniProtKB P19525.
ProteinModelPortal	Q6Z829.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os02g04240.1; LOC_Os02g04240.1; LOC_Os02g04240.
GeneID	4328224.
KEGG	dosa:Os02t0135300-00. osa:4328224.
Organism-specific databases
Gramene	Q6Z829.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000083236.
OMA	LLYQICK.
ProtClustDB	CLSN2919164.
Gene expression databases
ArrayExpress	Q6Z829.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WEE1_ORYSJ

Accession

Primary (citable) accession number: Q6Z829
Secondary accession number(s): Q0E458

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program