ID   GUN4_ORYSJ              Reviewed;         503 AA.
AC   Q6Z715; A0A0P0VE54; Q0E4D7;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   24-JAN-2024, entry version 113.
DE   RecName: Full=Endoglucanase 4;
DE            EC=3.2.1.4;
DE   AltName: Full=Endo-1,4-beta glucanase 4;
DE   AltName: Full=OsCel9F;
DE   AltName: Full=OsGLU14;
DE   Flags: Precursor;
GN   Name=GLU14; OrderedLocusNames=Os02g0123700, LOC_Os02g03120;
GN   ORFNames=P0575F10.17;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [5]
RP   INDUCTION.
RX   PubMed=17056619; DOI=10.1093/pcp/pcl021;
RA   Yoshida K., Imaizumi N., Kaneko S., Kawagoe Y., Tagiri A., Tanaka H.,
RA   Nishitani K., Komae K.;
RT   "Carbohydrate-binding module of a rice endo-beta-1,4-glycanase, OsCel9A,
RT   expressed in auxin-induced lateral root primordia, is post-translationally
RT   truncated.";
RL   Plant Cell Physiol. 47:1555-1571(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- INDUCTION: By auxin in roots. {ECO:0000269|PubMed:17056619}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR   EMBL; AP004885; BAD07956.1; -; Genomic_DNA.
DR   EMBL; AP008208; BAF07651.1; -; Genomic_DNA.
DR   EMBL; AP014958; BAS76739.1; -; Genomic_DNA.
DR   EMBL; AK106726; -; NOT_ANNOTATED_CDS; mRNA.
DR   AlphaFoldDB; Q6Z715; -.
DR   SMR; Q6Z715; -.
DR   STRING; 39947.Q6Z715; -.
DR   CAZy; GH9; Glycoside Hydrolase Family 9.
DR   PaxDb; 39947-Q6Z715; -.
DR   EnsemblPlants; Os02t0123700-01; Os02t0123700-01; Os02g0123700.
DR   Gramene; Os02t0123700-01; Os02t0123700-01; Os02g0123700.
DR   eggNOG; ENOG502QPI6; Eukaryota.
DR   HOGENOM; CLU_008926_1_2_1; -.
DR   InParanoid; Q6Z715; -.
DR   OMA; LVFRTCD; -.
DR   Proteomes; UP000000763; Chromosome 2.
DR   Proteomes; UP000059680; Chromosome 2.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR   InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR   PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR   PANTHER; PTHR22298:SF29; ENDOGLUCANASE; 1.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR   PROSITE; PS60032; GH9_1; 1.
DR   PROSITE; PS00592; GH9_2; 1.
DR   PROSITE; PS00698; GH9_3; 1.
DR   Genevisible; Q6Z715; OS.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW   Cellulose degradation; Glycosidase; Hydrolase; Polysaccharide degradation;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..503
FT                   /note="Endoglucanase 4"
FT                   /id="PRO_0000249281"
FT   ACT_SITE        77
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT   ACT_SITE        418
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        469
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   ACT_SITE        478
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   CONFLICT        39
FT                   /note="Q -> R (in Ref. 4; AK106726)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   503 AA;  53899 MW;  E9EA970F02FD62C0 CRC64;
     MSRACLLLVV AAVCLAGREA AAFNYADALD KAILFFEAQR SGKLPPGQRV AWRADSGLSD
     GSADGVDLAG GYYDAGDNVK FGLPMAFTVT MLSWSVIEFG DMMPARRSSF LGGIFGGGGV
     AQLDNARAAV RWGADYLLKA ATATPDTLYV QVADPYQDHR CWERPEDMDT PRSVYKVTPQ
     SPGSDVAGET AAALAAASIV FRVSDPSYSA KLLDAAQLVF DFADKYRGSY SDSLSSVVCP
     FYCSHSYHDE LLWAASWLHL ASPEKKDVYL SYIGSNGHAL GAEQDDFTFS WDDKRVATKG
     FLQSRADGLQ LYKAHTDNYI CSLVPGANGF QSQYTPGGLL FKEGDSNMQY VTSTAFLLLT
     YAKYLSSSAA TVSCGSTAVS PSTLISLAKK QVDYILGANP AGMSYMVGFG ARYPRHVHHR
     GASMPSVRDH PARIGCDEGF RYLHSPEPDR NLLAGAVVGG PDAGDAFADG RDNYAQAEPS
     TYTNAPLVGA LAFFAGAHKI FTP
//