Reviewed, UniProtKB/Swiss-Prot Q6Z715 (GUN4_ORYSJ)

Names and origin

Protein names

Recommended name:
    Endoglucanase 4
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta glucanase 4
    OsGLU14
    OsCel9F

Gene names

Name:	GLU14
Ordered Locus Names:	Os02g0123700, LOC_Os02g03120
ORF Names:	P0575F10.17

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Induction	By auxin in roots. Ref.4
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 503	481	Endoglucanase 4		PRO_0000249281
Sites
	Active site	418	1	By similarity
	Active site	469	1	By similarity
	Active site	478	1	By similarity
Experimental info
	Sequence conflict	39	1	Q → R in AK106726. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q6Z715-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: E9EA970F02FD62C0	FASTA	503	53,899
	10 20 30 40 50 60 MSRACLLLVV AAVCLAGREA AAFNYADALD KAILFFEAQR SGKLPPGQRV AWRADSGLSD 70 80 90 100 110 120 GSADGVDLAG GYYDAGDNVK FGLPMAFTVT MLSWSVIEFG DMMPARRSSF LGGIFGGGGV 130 140 150 160 170 180 AQLDNARAAV RWGADYLLKA ATATPDTLYV QVADPYQDHR CWERPEDMDT PRSVYKVTPQ 190 200 210 220 230 240 SPGSDVAGET AAALAAASIV FRVSDPSYSA KLLDAAQLVF DFADKYRGSY SDSLSSVVCP 250 260 270 280 290 300 FYCSHSYHDE LLWAASWLHL ASPEKKDVYL SYIGSNGHAL GAEQDDFTFS WDDKRVATKG 310 320 330 340 350 360 FLQSRADGLQ LYKAHTDNYI CSLVPGANGF QSQYTPGGLL FKEGDSNMQY VTSTAFLLLT 370 380 390 400 410 420 YAKYLSSSAA TVSCGSTAVS PSTLISLAKK QVDYILGANP AGMSYMVGFG ARYPRHVHHR 430 440 450 460 470 480 GASMPSVRDH PARIGCDEGF RYLHSPEPDR NLLAGAVVGG PDAGDAFADG RDNYAQAEPS 490 500 TYTNAPLVGA LAFFAGAHKI FTP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[4]	"Carbohydrate-binding module of a rice endo-beta-1,4-glycanase, OsCel9A, expressed in auxin-induced lateral root primordia, is post-translationally truncated." Yoshida K., Imaizumi N., Kaneko S., Kawagoe Y., Tagiri A., Tanaka H., Nishitani K., Komae K. Plant Cell Physiol. 47:1555-1571(2006) [PubMed: 17056619] [Abstract] Cited for: INDUCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP004885 Genomic DNA. Translation: BAD07956.1. AP008208 Genomic DNA. Translation: BAF07651.1. AK106726 mRNA. No translation available.
RefSeq	NP_001045737.1.
UniGene	Os.54770
3D structure databases
HSSP	HSSP built from PDB template 1K72 based on UniProtKB P37700.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Genome annotation databases
GeneID	4328141.
GenomeReviews	Gene locus GLU14 in contig AP008208_GR.
KEGG	osa:4328141.
Organism-specific databases
Gramene	Q6Z715.
Family and domain databases
InterPro	IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
PANTHER	PTHR22298:SF3. Glyco_hydro_9. 1 hit.
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN4_ORYSJ

Accession

Primary (citable) accession number: Q6Z715
Secondary accession number(s): Q0E4D7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	July 5, 2004
Last modified:	September 22, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)