Reviewed, UniProtKB/Swiss-Prot Q6Z6K4 (BGAL4_ORYSJ)

Names and origin

Protein names

Recommended name:
    Beta-galactosidase 4
      Short name=Lactase 4
    EC=3.2.1.23

Gene names

Ordered Locus Names:	Os02g0219200, LOC_Os02g12730
ORF Names:	P0027A02.24

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	729 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 35 family.
Sequence caution	The sequence BAF08224.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Apoplast Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	Potential
	Chain	36 – 729	694	Beta-galactosidase 4		PRO_0000294156
Sites
	Active site	194	1	Proton donor Potential
	Active site	263	1	Nucleophile Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6Z6K4-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 877E85CECA84EEEE	FASTA	729	79,996
	10 20 30 40 50 60 MAPAPTPAAA AGRRVAVLAA ALVAASLAAS VGVANAAVSY DRRSLVINGR RRILLSGSIH 70 80 90 100 110 120 YPRSTPEMWP GLIQKAKDGG LDVIQTYVFW NGHEPVQGQY YFSDRYDLVR FVKLVKQAGL 130 140 150 160 170 180 YVHLRIGPYV CAEWNFGGFP VWLKYVPGVS FRTDNGPFKA EMQKFVEKIV SMMKSEGLFE 190 200 210 220 230 240 WQGGPIIMSQ VENEFGPMES VGGSGAKPYA NWAAKMAVGT NTGVPWVMCK QDDAPDPVIN 250 260 270 280 290 300 TCNGFYCDYF SPNKNYKPSM WTEAWTGWFT SFGGGVPHRP VEDLAFAVAR FIQKGGSFVN 310 320 330 340 350 360 YYMYHGGTNF GRTAGGPFIA TSYDYDAPID EFGLLRQPKW GHLRDLHRAI KQAEPVLVSA 370 380 390 400 410 420 DPTIESIGSY EKAYVFKAKN GACAAFLSNY HMNTAVKVRF NGQQYNLPAW SISILPDCKT 430 440 450 460 470 480 AVFNTATVKE PTLMPKMNPV VRFAWQSYSE DTNSLSDSAF TKDGLVEQLS MTWDKSDYLW 490 500 510 520 530 540 YTTYVNIGTN DLRSGQSPQL TVYSAGHSMQ VFVNGKSYGS VYGGYDNPKL TYNGRVKMWQ 550 560 570 580 590 600 GSNKISILSS AVGLPNVGNH FENWNVGVLG PVTLSSLNGG TKDLSHQKWT YQVGLKGETL 610 620 630 640 650 660 GLHTVTGSSA VEWGGPGGYQ PLTWHKAFFN APAGNDPVAL DMGSMGKGQL WVNGHHVGRY 670 680 690 700 710 720 WSYKASGGCG GCSYAGTYHE DKCRSNCGDL SQRWYHVPRS WLKPGGNLLV VLEEYGGDLA GVSLATRTT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 230-729. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP004996 Genomic DNA. Translation: BAD17189.1. AP008208 Genomic DNA. Translation: BAF08224.1. Sequence problems. AK059059 mRNA. No translation available.
RefSeq	NP_001046310.1.
UniGene	Os.14358
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH35. Glycoside Hydrolase Family 35.
Genome annotation databases
GeneID	4328745.
KEGG	osa:4328745.
Organism-specific databases
Gramene	Q6Z6K4.
Family and domain databases
InterPro	IPR019801. Glyco_hydro_35_CS. IPR013781. Glyco_hydro_sg_catalytic. IPR001944. Glycoside_Hdrlase_35. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR23421. Glyco_hydro_35. 1 hit.
Pfam	PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL4_ORYSJ

Accession

Primary (citable) accession number: Q6Z6K4
Secondary accession number(s): Q0E2R4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 5, 2004
Last modified:	September 22, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)