Q6Z5P2 (GUN7_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 7
EC=3.2.1.4

Alternative name(s):
Endo-1,4-beta glucanase 7
OsGLU10

Gene names

Name:	GLU10
Ordered Locus Names:	Os02g0738600, LOC_Os02g50490
ORF Names:	P0684F11.11

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	531 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Tissue specificity	Ubiquitous. Ref.4
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.
Sequence caution	The sequence BAF09976.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 531	504	Endoglucanase 7		PRO_0000249284
Sites
	Active site	430	1	By similarity
	Active site	482	1	By similarity
	Active site	491	1	By similarity
Experimental info
	Sequence conflict	127	1	A → V in AK099698. Ref.3
	Sequence conflict	380	1	D → G in AK099698. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q6Z5P2 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 6E87230EDEA34F70	FASTA	531	58,598
	10 20 30 40 50 60 MRGRALVLVA ALLLQLLLLA AAGGAGAAAT ERKAHNYEDA LRKSLLYFEA QRSGRLPHNQ 70 80 90 100 110 120 RVAWRDHSGL TDGLEQGVDL VGGYYDAGDH VKFGLPMAFT VTMLSWSMIE YGDDVEAAGE 130 140 150 160 170 180 LGHALEAIKW GTDYFIKAHT KPNELWAEVG DGDTDHYCWQ RPEDMTTSRQ AYKVDRERPG 190 200 210 220 230 240 SDVAGETAAA MAAASIVFRK SNPHYASLLL HHAQQLFEFA DKYRGKYDSS IAEVKSYYAS 250 260 270 280 290 300 VSGYKDELLW AALWLHRATG KAHYLDYVVD NADCFGGTGW AITEFSWDVK YAGVQILAAR 310 320 330 340 350 360 LLLRGEHEER HRSTLEQYRA KAEHYVCGCL GRNADGGADA NVERSPGGML YVRQWNNMQY 370 380 390 400 410 420 VTNAAFLLAA YADYLGDDAD GAVSCAGGET AGAGEVAALA RAQVDYVLGT NPRGISYLVG 430 440 450 460 470 480 YGAKYPNRVH HRAASIVPYK HSKEFIGCTQ GFDHWFGRRS SNPNVLVGAI VGGPDRRDRF 490 500 510 520 530 RDNRENYMQT EACTYNTAPM VGMFAKLNRM ARQEREQEEV AAPARSTAAD V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[4]	"OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice, affects plant internode elongation." Zhou H.-L., He S.-J., Cao Y.-R., Chen T., Du B.-X., Chu C.-C., Zhang J.-S., Chen S.-Y. Plant Mol. Biol. 60:137-151(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP005112 Genomic DNA. Translation: BAD16040.1. AP008208 Genomic DNA. Translation: BAF09976.1. Different initiation. AK099698 mRNA. No translation available.
RefSeq	NP_001048062.1. NM_001054597.1.
UniGene	Os.17509.
3D structure databases
HSSP	HSSP built from PDB template 1TF4 based on UniProtKB P26221.
ProteinModelPortal	Q6Z5P2.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Proteomic databases
PaxDb	Q6Z5P2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4330667.
KEGG	dosa:Os02t0738600-01. dosa:Os06t0247900-01. osa:4330667.
Organism-specific databases
Gramene	Q6Z5P2.
Phylogenomic databases
eggNOG	NOG05134.
HOGENOM	HOG000021033.
ProtClustDB	CLSN2693172.
Gene expression databases
ArrayExpress	Q6Z5P2.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN7_ORYSJ

Accession

Primary (citable) accession number: Q6Z5P2
Secondary accession number(s): Q0DXR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program