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Protein

Mitogen-activated protein kinase 3

Gene

MPK3

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61ATPPROSITE-ProRule annotation1
Active sitei158Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 46ATPPROSITE-ProRule annotation9

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-OSA-110056. MAPK3 (ERK1) activation.
R-OSA-112409. RAF-independent MAPK1/3 activation.
R-OSA-112411. MAPK1 (ERK2) activation.
R-OSA-198753. ERK/MAPK targets.
R-OSA-198765. Signalling to ERK5.
R-OSA-202670. ERKs are inactivated.
R-OSA-3371453. Regulation of HSF1-mediated heat shock response.
R-OSA-442742. CREB phosphorylation through the activation of Ras.
R-OSA-445144. Signal transduction by L1.
R-OSA-5673001. RAF/MAP kinase cascade.
R-OSA-5674135. MAP2K and MAPK activation.
R-OSA-5674499. Negative feedback regulation of MAPK pathway.
R-OSA-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 3 (EC:2.7.11.24)
Short name:
MAP kinase 3
Alternative name(s):
MAP kinase 2
OsMAP3
OsMAPK2
Gene namesi
Name:MPK3
Synonyms:MAP3, MAPK2
Ordered Locus Names:Os02g0148100, LOC_Os02g05480
ORF Names:P0479D12.11
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002397461 – 370Mitogen-activated protein kinase 3Add BLAST370

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei191PhosphothreonineBy similarity1
Modified residuei193PhosphotyrosineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6Z437.
PRIDEiQ6Z437.

Expressioni

Gene expression databases

GenevisibleiQ6Z437. OS.

Interactioni

Protein-protein interaction databases

IntActiQ6Z437. 5 interactors.
STRINGi39947.LOC_Os02g05480.1.

Structurei

3D structure databases

ProteinModelPortaliQ6Z437.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 319Protein kinasePROSITE-ProRule annotationAdd BLAST288

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi191 – 193TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
InParanoidiQ6Z437.
KOiK20535.
OMAiNNAFENR.
OrthoDBiEOG09360CTC.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6Z437-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIMVDPPNG MGNQGKYYYS MWQTLFEIDT KYVPIKPIGR GAYGIVCSSI
60 70 80 90 100
NRETNEKVAI KKIHNVFDNR VDALRTLREL KLLRHLRHEN VIALKDIMMP
110 120 130 140 150
VHRRSFKDVY LVYELMDTDL HQIIKSPQGL SNDHCQYFLF QLLRGLKYLH
160 170 180 190 200
SAEILHRDLK PGNLLVNANC DLKICDFGLA RTNSSKGQFM TEYVVTRWYR
210 220 230 240 250
APELLLCCDN YGTSIDVWSV GCIFAELLGR KPIFPGTECL NQLKLIVNVL
260 270 280 290 300
GTMSESDLEF IDNPKARRYI KSLPYTPGVP LASMYPHAHP LAIDLLQKML
310 320 330 340 350
IFDPTKRISV TEALEHPYMS PLYDPSANPP AQVPIDLDID ENISADMIRE
360 370
MMWHEMLHYH PEVVAAMSAR
Length:370
Mass (Da):42,469
Last modified:July 5, 2004 - v1
Checksum:i7F03ACCA17F0E033
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216317 mRNA. Translation: AAG40581.1.
AF241166 mRNA. Translation: AAF61238.1.
AP005191 Genomic DNA. Translation: BAD13057.1.
AP008208 Genomic DNA. Translation: BAF07803.1.
AP014958 Genomic DNA. Translation: BAS76982.1.
AK119650 mRNA. Translation: BAG99730.1.
RefSeqiXP_015624723.1. XM_015769237.1.
XP_015624724.1. XM_015769238.1.
UniGeneiOs.15659.

Genome annotation databases

EnsemblPlantsiOS02T0148100-01; OS02T0148100-01; OS02G0148100.
GeneIDi4328297.
GrameneiOS02T0148100-01; OS02T0148100-01; OS02G0148100.
KEGGiosa:4328297.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216317 mRNA. Translation: AAG40581.1.
AF241166 mRNA. Translation: AAF61238.1.
AP005191 Genomic DNA. Translation: BAD13057.1.
AP008208 Genomic DNA. Translation: BAF07803.1.
AP014958 Genomic DNA. Translation: BAS76982.1.
AK119650 mRNA. Translation: BAG99730.1.
RefSeqiXP_015624723.1. XM_015769237.1.
XP_015624724.1. XM_015769238.1.
UniGeneiOs.15659.

3D structure databases

ProteinModelPortaliQ6Z437.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6Z437. 5 interactors.
STRINGi39947.LOC_Os02g05480.1.

Proteomic databases

PaxDbiQ6Z437.
PRIDEiQ6Z437.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS02T0148100-01; OS02T0148100-01; OS02G0148100.
GeneIDi4328297.
GrameneiOS02T0148100-01; OS02T0148100-01; OS02G0148100.
KEGGiosa:4328297.

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
InParanoidiQ6Z437.
KOiK20535.
OMAiNNAFENR.
OrthoDBiEOG09360CTC.

Enzyme and pathway databases

ReactomeiR-OSA-110056. MAPK3 (ERK1) activation.
R-OSA-112409. RAF-independent MAPK1/3 activation.
R-OSA-112411. MAPK1 (ERK2) activation.
R-OSA-198753. ERK/MAPK targets.
R-OSA-198765. Signalling to ERK5.
R-OSA-202670. ERKs are inactivated.
R-OSA-3371453. Regulation of HSF1-mediated heat shock response.
R-OSA-442742. CREB phosphorylation through the activation of Ras.
R-OSA-445144. Signal transduction by L1.
R-OSA-5673001. RAF/MAP kinase cascade.
R-OSA-5674135. MAP2K and MAPK activation.
R-OSA-5674499. Negative feedback regulation of MAPK pathway.
R-OSA-6798695. Neutrophil degranulation.

Gene expression databases

GenevisibleiQ6Z437. OS.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPK3_ORYSJ
AccessioniPrimary (citable) accession number: Q6Z437
Secondary accession number(s): Q0E3Y5, Q9M545
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.