Q6Z398 (HXK4_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hexokinase-4, chloroplastic
EC=2.7.1.1

Alternative name(s):
Hexokinase II

Gene names

Name:	HXK4
Ordered Locus Names:	Os07g0197100, LOC_Os07g09890
ORF Names:	P0417F02.7, P0589E08.26

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Fructose and glucose phosphorylating enzyme. Ref.1
Catalytic activity	ATP + D-hexose = ADP + D-hexose 6-phosphate.
Subcellular location	Plastid › chloroplast stroma Probable Ref.1.
Tissue specificity	Expressed in roots, leaves, flowers, immature seeds, endosperm and seed coat. Ref.1
Developmental stage	Expressed during flower development until 8 days after flowering. Ref.1
Induction	Not induced by glucose or fructose treatment in leaves. Ref.1
Sequence similarities	Belongs to the hexokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glucose 6-phosphate metabolic process Inferred from electronic annotation. Source: GOC glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructokinase activity Inferred from electronic annotation. Source: EnsemblPlants/Gramene glucokinase activity Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 37	37	Chloroplast Potential
	Chain	38 – 509	472	Hexokinase-4, chloroplastic		PRO_0000247567
Regions
	Nucleotide binding	111 – 116	6	ATP Potential
	Region	181 – 207	27	Glucose-binding Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6Z398 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C1DCA84034804BF8	FASTA	509	54,759
	10 20 30 40 50 60 MSAAAAIASP IPAAIAVVQQ QRRGRSRGGG SGAAAVRCSA VAPTSAIAPI LADLRLRCAA 70 80 90 100 110 120 PLPVLRRVAD AMASGMRAGL ADDGAGELKM IPSHVYSLPT GNETGLFYAL DLGGTNFRVL 130 140 150 160 170 180 RVQLGGKDKR IIDTEFEQVS IPREIMHGIT EDLFDFIASG LSRFVATEGD KFHLPQGRKR 190 200 210 220 230 240 ELGFTFSFPV NQTSIDSGIL IKWTKGFAVS GTAGKDVVAC LNAAMERQGL DMRVSALVND 250 260 270 280 290 300 TVGTLAGARY WDDDVMVAVI LGTGTNACYI QRTEAIPKLQ HLKLETGNTI INTEWGAFSD 310 320 330 340 350 360 GLPLTEFDRE MDDESINPGE QIFEKTISGM YLGEIVRRVL VKMAEVSDLF GHSFPKKLAE 370 380 390 400 410 420 PFVLRTPHLC AMQQDTSDNL GEVESILSDV IGVSQASLLA RRVTVEVSDC IIRRGGRLAG 430 440 450 460 470 480 AGIVGILEKM ENDSRGHIFG RRTVVAMDGG LYEKYPQYRR YMKEAVAELL GPERSNRIAI 490 500 EHTKDGSGIG AALLAAANSK YAAAQISTR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure, expression, and functional analysis of the hexokinase gene family in rice (Oryza sativa L.)." Cho J.-I., Ryoo N., Ko S., Lee S.-K., Lee J., Jung K.-H., Lee Y.-H., Bhoo S.H., Winderickx J., An G., Hahn T.-R., Jeon J.-S. Planta 224:598-611(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, NOMENCLATURE. Strain: cv. Jinmi.
[2]	"Rice hexokinase II mRNA." Wu P., Jiang H.-W., Yi K.-K. Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ116386 mRNA. Translation: AAZ93621.1. AF372832 mRNA. Translation: AAK51560.1. AP004379 Genomic DNA. Translation: BAD30694.1. AP005257 Genomic DNA. Translation: BAC84178.1.
RefSeq	NP_001059124.1. NM_001065659.1.
UniGene	Os.3294.
3D structure databases
ProteinModelPortal	Q6Z398.
ModBase	Search...
Proteomic databases
PRIDE	Q6Z398.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os07g09890.1; LOC_Os07g09890.1; LOC_Os07g09890.
GeneID	4342654.
KEGG	dosa:Os07t0197100-01. osa:4342654.
Organism-specific databases
Gramene	Q6Z398.
Phylogenomic databases
eggNOG	COG5026.
HOGENOM	HOG000162670.
KO	K00844.
OMA	MDSQICA.
ProtClustDB	PLN02914.
Enzyme and pathway databases
BRENDA	2.7.1.1. 4460.
Gene expression databases
ArrayExpress	Q6Z398.
Family and domain databases
InterPro	IPR001312. Hexokinase. IPR022673. Hexokinase_C. IPR019807. Hexokinase_CS. IPR022672. Hexokinase_N. [Graphical view]
PANTHER	PTHR19443. PTHR19443. 1 hit.
Pfam	PF00349. Hexokinase_1. 1 hit. PF03727. Hexokinase_2. 1 hit. [Graphical view]
PRINTS	PR00475. HEXOKINASE.
PROSITE	PS00378. HEXOKINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HXK4_ORYSJ

Accession

Primary (citable) accession number: Q6Z398
Secondary accession number(s): Q94JW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program