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Protein

Eukaryotic initiation factor 4A-3

Gene

Os02g0146600

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi85 – 92ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, Initiation factor, RNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-OSA-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-OSA-72662 Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S
R-OSA-72702 Ribosomal scanning and start codon recognition

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic initiation factor 4A-3 (EC:3.6.4.13)
Short name:
eIF-4A-3
Alternative name(s):
ATP-dependent RNA helicase eIF4A-3
DEAD-box ATP-dependent RNA helicase 23
eIF4A-2
Gene namesi
Ordered Locus Names:Os02g0146600, LOC_Os02g05330
ORF Names:OJ1008_C03.10, OsJ_05367Imported
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002824411 – 414Eukaryotic initiation factor 4A-3Add BLAST414

Proteomic databases

PaxDbiQ6Z2Z4
PRIDEiQ6Z2Z4

Expressioni

Gene expression databases

ExpressionAtlasiQ6Z2Z4 differential
GenevisibleiQ6Z2Z4 OS

Interactioni

Subunit structurei

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G (By similarity). Interacts with DRM2 (via UBA domains) (PubMed:23732981).By similarity1 Publication

Protein-protein interaction databases

STRINGi39947.LOC_Os02g05330.1

Structurei

3D structure databases

ProteinModelPortaliQ6Z2Z4
SMRiQ6Z2Z4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 242Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST171
Domaini253 – 414Helicase C-terminalPROSITE-ProRule annotationAdd BLAST162

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi41 – 69Q motifAdd BLAST29
Motifi190 – 193DEAD box4

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0327 Eukaryota
COG0513 LUCA
HOGENOMiHOG000268797
InParanoidiQ6Z2Z4
KOiK03257
OMAiFTHRNGR
OrthoDBiEOG09360ASW

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequencei

Sequence statusi: Complete.

Q6Z2Z4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGMAPEGSQ FDAKHYDSKM QELLHQGDNE EFFTSYDEVF ESFDDMGLQE
60 70 80 90 100
NLLRGIYAYG FEKPSAIQQR GIVPFCKGLD VIQQAQSGTG KTATFCSGIL
110 120 130 140 150
QQLDYGLVEC QSLVLAPTRE LAQQIEKVMR ALGDYLGVKV HACVGGTSVR
160 170 180 190 200
EDQRILASGV HVVVGTPGRV FDMLRRQSLR PDHIKMFVLD EADEMLSRGF
210 220 230 240 250
KDQIYDIFQL LPPKIQVGVF SATMPPEALE ITRKFMNKPV RILVKRDELT
260 270 280 290 300
LEGIKQFYVN VEKEDWKLDT LCDLYETLAI TQSVIFVNTR RKVDWLTDKM
310 320 330 340 350
RSRDHTVSAT HGDMDQNTRD IIMREFRSGS SRVLITTDLL ARGIDVQQVS
360 370 380 390 400
LVINYDLPTQ PENYLHRIGR SGRFGRKGVA INFVTRDDER MLFDIQRFYN
410
VTIEELPANV ADLL
Length:414
Mass (Da):47,138
Last modified:July 5, 2004 - v1
Checksum:iB440EF6727D791DD
GO

Sequence cautioni

The sequence BAB21258 differs from that shown. Reason: Frameshift at positions 31 and 35.Curated
The sequence BAB21259 differs from that shown. Reason: Frameshift at positions 31 and 35.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38 – 39EV → DL in BAB21258 (PubMed:11913775).Curated2
Sequence conflicti38 – 39EV → DL in BAB21259 (PubMed:11913775).Curated2
Sequence conflicti69Q → K in BAB21258 (PubMed:11913775).Curated1
Sequence conflicti69Q → K in BAB21259 (PubMed:11913775).Curated1
Sequence conflicti152D → Q in BAB21258 (PubMed:11913775).Curated1
Sequence conflicti152D → Q in BAB21259 (PubMed:11913775).Curated1
Sequence conflicti228A → G in BAB21258 (PubMed:11913775).Curated1
Sequence conflicti228A → G in BAB21259 (PubMed:11913775).Curated1
Sequence conflicti233 – 234RK → LR in BAB21258 (PubMed:11913775).Curated2
Sequence conflicti233 – 234RK → LR in BAB21259 (PubMed:11913775).Curated2
Sequence conflicti237N → D in BAB21258 (PubMed:11913775).Curated1
Sequence conflicti237N → D in BAB21259 (PubMed:11913775).Curated1
Sequence conflicti251L → R in BAB21258 (PubMed:11913775).Curated1
Sequence conflicti251L → R in BAB21259 (PubMed:11913775).Curated1
Sequence conflicti276 – 277ET → DS in BAB21258 (PubMed:11913775).Curated2
Sequence conflicti276 – 277ET → DS in BAB21259 (PubMed:11913775).Curated2
Sequence conflicti290 – 292RRK → LRM in BAB21258 (PubMed:11913775).Curated3
Sequence conflicti290 – 292RRK → LRM in BAB21259 (PubMed:11913775).Curated3
Sequence conflicti317N → I in BAB21258 (PubMed:11913775).Curated1
Sequence conflicti317N → I in BAB21259 (PubMed:11913775).Curated1
Sequence conflicti341A → D in BAB21258 (PubMed:11913775).Curated1
Sequence conflicti341A → D in BAB21259 (PubMed:11913775).Curated1
Sequence conflicti358P → S in BAB21258 (PubMed:11913775).Curated1
Sequence conflicti358P → S in BAB21259 (PubMed:11913775).Curated1
Sequence conflicti361P → T in BAB21258 (PubMed:11913775).Curated1
Sequence conflicti361P → T in BAB21259 (PubMed:11913775).Curated1
Sequence conflicti382N → I in BAB21258 (PubMed:11913775).Curated1
Sequence conflicti382N → I in BAB21259 (PubMed:11913775).Curated1
Sequence conflicti401V → G in BAB21258 (PubMed:11913775).Curated1
Sequence conflicti401V → G in BAB21259 (PubMed:11913775).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046414 mRNA Translation: BAB21258.1 Frameshift.
AB046415 Genomic DNA Translation: BAB21259.1 Frameshift.
KC140119 mRNA Translation: AGT42311.1
AP005288 Genomic DNA Translation: BAD13081.1
AP008208 Genomic DNA Translation: BAF07792.1
AP014958 Genomic DNA Translation: BAS76965.1
CM000139 Genomic DNA Translation: EEE56296.1
AK073620 mRNA Translation: BAG93556.1
RefSeqiXP_015626460.1, XM_015770974.1
UniGeneiOs.458

Genome annotation databases

EnsemblPlantsiOs02t0146600-01; Os02t0146600-01; Os02g0146600
GeneIDi4328286
GrameneiOs02t0146600-01; Os02t0146600-01; Os02g0146600
KEGGiosa:4328286

Similar proteinsi

Entry informationi

Entry nameiIF4A3_ORYSJ
AccessioniPrimary (citable) accession number: Q6Z2Z4
Secondary accession number(s): B7EMF9, Q9AR32
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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