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Q6Z2Z4 (IF4A3_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Eukaryotic initiation factor 4A-3

Short name=eIF-4A-3
EC=3.6.4.13
Alternative name(s):
ATP-dependent RNA helicase eIF4A-3
DEAD-box ATP-dependent RNA helicase 23
eIF4A-2
Gene names
Ordered Locus Names:Os02g0146600, LOC_Os02g05330
ORF Names:OJ1008_C03.10
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length414 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G By similarity.

Subcellular location

Cytoplasm By similarity.

Domain

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similarities

Belongs to the DEAD box helicase family. eIF4A subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Sequence caution

The sequence BAB21258.1 differs from that shown. Reason: Frameshift at positions 31 and 35.

The sequence BAB21259.1 differs from that shown. Reason: Frameshift at positions 31 and 35.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
RNA-binding
   Molecular functionHelicase
Hydrolase
Initiation factor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATP-dependent helicase activity

Inferred from electronic annotation. Source: InterPro

translation initiation factor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 414414Eukaryotic initiation factor 4A-3
PRO_0000282441

Regions

Domain72 – 242171Helicase ATP-binding
Domain253 – 414162Helicase C-terminal
Nucleotide binding85 – 928ATP By similarity
Motif41 – 6929Q motif
Motif190 – 1934DEAD box

Experimental info

Sequence conflict38 – 392EV → DL in BAB21258. Ref.1
Sequence conflict38 – 392EV → DL in BAB21259. Ref.1
Sequence conflict691Q → K in BAB21258. Ref.1
Sequence conflict691Q → K in BAB21259. Ref.1
Sequence conflict1521D → Q in BAB21258. Ref.1
Sequence conflict1521D → Q in BAB21259. Ref.1
Sequence conflict2281A → G in BAB21258. Ref.1
Sequence conflict2281A → G in BAB21259. Ref.1
Sequence conflict233 – 2342RK → LR in BAB21258. Ref.1
Sequence conflict233 – 2342RK → LR in BAB21259. Ref.1
Sequence conflict2371N → D in BAB21258. Ref.1
Sequence conflict2371N → D in BAB21259. Ref.1
Sequence conflict2511L → R in BAB21258. Ref.1
Sequence conflict2511L → R in BAB21259. Ref.1
Sequence conflict276 – 2772ET → DS in BAB21258. Ref.1
Sequence conflict276 – 2772ET → DS in BAB21259. Ref.1
Sequence conflict290 – 2923RRK → LRM in BAB21258. Ref.1
Sequence conflict290 – 2923RRK → LRM in BAB21259. Ref.1
Sequence conflict3171N → I in BAB21258. Ref.1
Sequence conflict3171N → I in BAB21259. Ref.1
Sequence conflict3411A → D in BAB21258. Ref.1
Sequence conflict3411A → D in BAB21259. Ref.1
Sequence conflict3581P → S in BAB21258. Ref.1
Sequence conflict3581P → S in BAB21259. Ref.1
Sequence conflict3611P → T in BAB21258. Ref.1
Sequence conflict3611P → T in BAB21259. Ref.1
Sequence conflict3821N → I in BAB21258. Ref.1
Sequence conflict3821N → I in BAB21259. Ref.1
Sequence conflict4011V → G in BAB21258. Ref.1
Sequence conflict4011V → G in BAB21259. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q6Z2Z4 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: B440EF6727D791DD

FASTA41447,138
        10         20         30         40         50         60 
MAGMAPEGSQ FDAKHYDSKM QELLHQGDNE EFFTSYDEVF ESFDDMGLQE NLLRGIYAYG 

        70         80         90        100        110        120 
FEKPSAIQQR GIVPFCKGLD VIQQAQSGTG KTATFCSGIL QQLDYGLVEC QSLVLAPTRE 

       130        140        150        160        170        180 
LAQQIEKVMR ALGDYLGVKV HACVGGTSVR EDQRILASGV HVVVGTPGRV FDMLRRQSLR 

       190        200        210        220        230        240 
PDHIKMFVLD EADEMLSRGF KDQIYDIFQL LPPKIQVGVF SATMPPEALE ITRKFMNKPV 

       250        260        270        280        290        300 
RILVKRDELT LEGIKQFYVN VEKEDWKLDT LCDLYETLAI TQSVIFVNTR RKVDWLTDKM 

       310        320        330        340        350        360 
RSRDHTVSAT HGDMDQNTRD IIMREFRSGS SRVLITTDLL ARGIDVQQVS LVINYDLPTQ 

       370        380        390        400        410 
PENYLHRIGR SGRFGRKGVA INFVTRDDER MLFDIQRFYN VTIEELPANV ADLL 

« Hide

References

« Hide 'large scale' references
[1]"Identification and characterization of two genes encoding the eukaryotic initiation factor 4A in rice."
Kato A., Fujita S., Komeda Y.
DNA Seq. 12:295-303(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[4]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB046414 mRNA. Translation: BAB21258.1. Frameshift.
AB046415 Genomic DNA. Translation: BAB21259.1. Frameshift.
AP005288 Genomic DNA. Translation: BAD13081.1.
AP008208 Genomic DNA. Translation: BAF07792.1.
AK073620 mRNA. No translation available.
RefSeqNP_001045878.1. NM_001052413.1.
UniGeneOs.458.

3D structure databases

ProteinModelPortalQ6Z2Z4.
SMRQ6Z2Z4. Positions 31-414.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ6Z2Z4.
PRIDEQ6Z2Z4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS02T0146600-01; OS02T0146600-01; OS02G0146600.
GeneID4328286.
KEGGosa:4328286.

Organism-specific databases

GrameneQ6Z2Z4.

Phylogenomic databases

eggNOGCOG0513.
HOGENOMHOG000268797.
KOK03257.
OMACIKGRYD.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIF4A3_ORYSJ
AccessionPrimary (citable) accession number: Q6Z2Z4
Secondary accession number(s): Q9AR32
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

Translation initiation factors

List of translation initiation factor entries