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Protein

Eukaryotic initiation factor 4A-3

Gene

Os02g0146600

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi85 – 92ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic initiation factor 4A-3 (EC:3.6.4.13)
Short name:
eIF-4A-3
Alternative name(s):
ATP-dependent RNA helicase eIF4A-3
DEAD-box ATP-dependent RNA helicase 23
eIF4A-2
Gene namesi
Ordered Locus Names:Os02g0146600, LOC_Os02g05330
ORF Names:OJ1008_C03.10, OsJ_05367Imported
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 2

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus 1 Publication

  • Note: Localizes to the nucleus when intertacting with DRM2.1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002824411 – 414Eukaryotic initiation factor 4A-3Add BLAST414

Proteomic databases

PaxDbiQ6Z2Z4.
PRIDEiQ6Z2Z4.

Expressioni

Gene expression databases

ExpressionAtlasiQ6Z2Z4. baseline and differential.
GenevisibleiQ6Z2Z4. OS.

Interactioni

Subunit structurei

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G (By similarity). Interacts with DRM2 (via UBA domains) (PubMed:23732981).By similarity1 Publication

Protein-protein interaction databases

STRINGi39947.LOC_Os02g05330.1.

Structurei

3D structure databases

ProteinModelPortaliQ6Z2Z4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 242Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST171
Domaini253 – 414Helicase C-terminalPROSITE-ProRule annotationAdd BLAST162

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi41 – 69Q motifAdd BLAST29
Motifi190 – 193DEAD box4

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0327. Eukaryota.
COG0513. LUCA.
HOGENOMiHOG000268797.
InParanoidiQ6Z2Z4.
KOiK03257.
OMAiKLKQKTH.
OrthoDBiEOG09360ASW.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6Z2Z4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGMAPEGSQ FDAKHYDSKM QELLHQGDNE EFFTSYDEVF ESFDDMGLQE
60 70 80 90 100
NLLRGIYAYG FEKPSAIQQR GIVPFCKGLD VIQQAQSGTG KTATFCSGIL
110 120 130 140 150
QQLDYGLVEC QSLVLAPTRE LAQQIEKVMR ALGDYLGVKV HACVGGTSVR
160 170 180 190 200
EDQRILASGV HVVVGTPGRV FDMLRRQSLR PDHIKMFVLD EADEMLSRGF
210 220 230 240 250
KDQIYDIFQL LPPKIQVGVF SATMPPEALE ITRKFMNKPV RILVKRDELT
260 270 280 290 300
LEGIKQFYVN VEKEDWKLDT LCDLYETLAI TQSVIFVNTR RKVDWLTDKM
310 320 330 340 350
RSRDHTVSAT HGDMDQNTRD IIMREFRSGS SRVLITTDLL ARGIDVQQVS
360 370 380 390 400
LVINYDLPTQ PENYLHRIGR SGRFGRKGVA INFVTRDDER MLFDIQRFYN
410
VTIEELPANV ADLL
Length:414
Mass (Da):47,138
Last modified:July 5, 2004 - v1
Checksum:iB440EF6727D791DD
GO

Sequence cautioni

The sequence BAB21258 differs from that shown. Reason: Frameshift at positions 31 and 35.Curated
The sequence BAB21259 differs from that shown. Reason: Frameshift at positions 31 and 35.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38 – 39EV → DL in BAB21258 (PubMed:11913775).Curated2
Sequence conflicti38 – 39EV → DL in BAB21259 (PubMed:11913775).Curated2
Sequence conflicti69Q → K in BAB21258 (PubMed:11913775).Curated1
Sequence conflicti69Q → K in BAB21259 (PubMed:11913775).Curated1
Sequence conflicti152D → Q in BAB21258 (PubMed:11913775).Curated1
Sequence conflicti152D → Q in BAB21259 (PubMed:11913775).Curated1
Sequence conflicti228A → G in BAB21258 (PubMed:11913775).Curated1
Sequence conflicti228A → G in BAB21259 (PubMed:11913775).Curated1
Sequence conflicti233 – 234RK → LR in BAB21258 (PubMed:11913775).Curated2
Sequence conflicti233 – 234RK → LR in BAB21259 (PubMed:11913775).Curated2
Sequence conflicti237N → D in BAB21258 (PubMed:11913775).Curated1
Sequence conflicti237N → D in BAB21259 (PubMed:11913775).Curated1
Sequence conflicti251L → R in BAB21258 (PubMed:11913775).Curated1
Sequence conflicti251L → R in BAB21259 (PubMed:11913775).Curated1
Sequence conflicti276 – 277ET → DS in BAB21258 (PubMed:11913775).Curated2
Sequence conflicti276 – 277ET → DS in BAB21259 (PubMed:11913775).Curated2
Sequence conflicti290 – 292RRK → LRM in BAB21258 (PubMed:11913775).Curated3
Sequence conflicti290 – 292RRK → LRM in BAB21259 (PubMed:11913775).Curated3
Sequence conflicti317N → I in BAB21258 (PubMed:11913775).Curated1
Sequence conflicti317N → I in BAB21259 (PubMed:11913775).Curated1
Sequence conflicti341A → D in BAB21258 (PubMed:11913775).Curated1
Sequence conflicti341A → D in BAB21259 (PubMed:11913775).Curated1
Sequence conflicti358P → S in BAB21258 (PubMed:11913775).Curated1
Sequence conflicti358P → S in BAB21259 (PubMed:11913775).Curated1
Sequence conflicti361P → T in BAB21258 (PubMed:11913775).Curated1
Sequence conflicti361P → T in BAB21259 (PubMed:11913775).Curated1
Sequence conflicti382N → I in BAB21258 (PubMed:11913775).Curated1
Sequence conflicti382N → I in BAB21259 (PubMed:11913775).Curated1
Sequence conflicti401V → G in BAB21258 (PubMed:11913775).Curated1
Sequence conflicti401V → G in BAB21259 (PubMed:11913775).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046414 mRNA. Translation: BAB21258.1. Frameshift.
AB046415 Genomic DNA. Translation: BAB21259.1. Frameshift.
KC140119 mRNA. Translation: AGT42311.1.
AP005288 Genomic DNA. Translation: BAD13081.1.
AP008208 Genomic DNA. Translation: BAF07792.1.
AP014958 Genomic DNA. Translation: BAS76965.1.
CM000139 Genomic DNA. Translation: EEE56296.1.
AK073620 mRNA. Translation: BAG93556.1.
RefSeqiXP_015626460.1. XM_015770974.1.
UniGeneiOs.458.

Genome annotation databases

EnsemblPlantsiOS02T0146600-01; OS02T0146600-01; OS02G0146600.
GeneIDi4328286.
GrameneiOS02T0146600-01; OS02T0146600-01; OS02G0146600.
KEGGiosa:4328286.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046414 mRNA. Translation: BAB21258.1. Frameshift.
AB046415 Genomic DNA. Translation: BAB21259.1. Frameshift.
KC140119 mRNA. Translation: AGT42311.1.
AP005288 Genomic DNA. Translation: BAD13081.1.
AP008208 Genomic DNA. Translation: BAF07792.1.
AP014958 Genomic DNA. Translation: BAS76965.1.
CM000139 Genomic DNA. Translation: EEE56296.1.
AK073620 mRNA. Translation: BAG93556.1.
RefSeqiXP_015626460.1. XM_015770974.1.
UniGeneiOs.458.

3D structure databases

ProteinModelPortaliQ6Z2Z4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os02g05330.1.

Proteomic databases

PaxDbiQ6Z2Z4.
PRIDEiQ6Z2Z4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS02T0146600-01; OS02T0146600-01; OS02G0146600.
GeneIDi4328286.
GrameneiOS02T0146600-01; OS02T0146600-01; OS02G0146600.
KEGGiosa:4328286.

Phylogenomic databases

eggNOGiKOG0327. Eukaryota.
COG0513. LUCA.
HOGENOMiHOG000268797.
InParanoidiQ6Z2Z4.
KOiK03257.
OMAiKLKQKTH.
OrthoDBiEOG09360ASW.

Gene expression databases

ExpressionAtlasiQ6Z2Z4. baseline and differential.
GenevisibleiQ6Z2Z4. OS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF4A3_ORYSJ
AccessioniPrimary (citable) accession number: Q6Z2Z4
Secondary accession number(s): B7EMF9, Q9AR32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.