Eukaryotic initiation factor 4A-3 - Oryza sativa subsp. japonica (Rice)

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Q6Z2Z4 (IF4A3_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 65. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Eukaryotic initiation factor 4A-3
Short name=eIF-4A-3
EC=3.6.4.13

Alternative name(s):
ATP-dependent RNA helicase eIF4A-3
DEAD-box ATP-dependent RNA helicase 23
eIF4A-2

Gene names

Ordered Locus Names:	Os02g0146600, LOC_Os02g05330
ORF Names:	OJ1008_C03.10

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza ›

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon By similarity.
Catalytic activity	ATP + H₂O = ADP + phosphate.
Subunit structure	eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.
Sequence similarities	Belongs to the DEAD box helicase family. eIF4A subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Sequence caution	The sequence BAB21258.1 differs from that shown. Reason: Frameshift at positions 31 and 35. The sequence BAB21259.1 differs from that shown. Reason: Frameshift at positions 31 and 35.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase Initiation factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to cadmium ion Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	cytosol Inferred from electronic annotation. Source: EnsemblPlants/Gramene nucleolus Inferred from electronic annotation. Source: EnsemblPlants/Gramene plant-type cell wall Inferred from electronic annotation. Source: EnsemblPlants/Gramene plasma membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro translation initiation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 414

414

Eukaryotic initiation factor 4A-3

PRO_0000282441

Regions

Domain

72 – 242

171

Helicase ATP-binding

Domain

253 – 414

162

Helicase C-terminal

Nucleotide binding

85 – 92

ATP By similarity

Motif

41 – 69

Q motif

Motif

190 – 193

DEAD box

Experimental info

Sequence conflict

38 – 39

EV → DL in BAB21258. Ref.1

Sequence conflict

38 – 39

EV → DL in BAB21259. Ref.1

Sequence conflict

Q → K in BAB21258. Ref.1

Sequence conflict

Q → K in BAB21259. Ref.1

Sequence conflict

152

D → Q in BAB21258. Ref.1

Sequence conflict

152

D → Q in BAB21259. Ref.1

Sequence conflict

228

A → G in BAB21258. Ref.1

Sequence conflict

228

A → G in BAB21259. Ref.1

Sequence conflict

233 – 234

RK → LR in BAB21258. Ref.1

Sequence conflict

233 – 234

RK → LR in BAB21259. Ref.1

Sequence conflict

237

N → D in BAB21258. Ref.1

Sequence conflict

237

N → D in BAB21259. Ref.1

Sequence conflict

251

L → R in BAB21258. Ref.1

Sequence conflict

251

L → R in BAB21259. Ref.1

Sequence conflict

276 – 277

ET → DS in BAB21258. Ref.1

Sequence conflict

276 – 277

ET → DS in BAB21259. Ref.1

Sequence conflict

290 – 292

RRK → LRM in BAB21258. Ref.1

Sequence conflict

290 – 292

RRK → LRM in BAB21259. Ref.1

Sequence conflict

317

N → I in BAB21258. Ref.1

Sequence conflict

317

N → I in BAB21259. Ref.1

Sequence conflict

341

A → D in BAB21258. Ref.1

Sequence conflict

341

A → D in BAB21259. Ref.1

Sequence conflict

358

P → S in BAB21258. Ref.1

Sequence conflict

358

P → S in BAB21259. Ref.1

Sequence conflict

361

P → T in BAB21258. Ref.1

Sequence conflict

361

P → T in BAB21259. Ref.1

Sequence conflict

382

N → I in BAB21258. Ref.1

Sequence conflict

382

N → I in BAB21259. Ref.1

Sequence conflict

401

V → G in BAB21258. Ref.1

Sequence conflict

401

V → G in BAB21259. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q6Z2Z4 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: B440EF6727D791DD
Show »

FASTA

414

47,138

        10         20         30         40         50         60 
MAGMAPEGSQ FDAKHYDSKM QELLHQGDNE EFFTSYDEVF ESFDDMGLQE NLLRGIYAYG 

        70         80         90        100        110        120 
FEKPSAIQQR GIVPFCKGLD VIQQAQSGTG KTATFCSGIL QQLDYGLVEC QSLVLAPTRE 

       130        140        150        160        170        180 
LAQQIEKVMR ALGDYLGVKV HACVGGTSVR EDQRILASGV HVVVGTPGRV FDMLRRQSLR 

       190        200        210        220        230        240 
PDHIKMFVLD EADEMLSRGF KDQIYDIFQL LPPKIQVGVF SATMPPEALE ITRKFMNKPV 

       250        260        270        280        290        300 
RILVKRDELT LEGIKQFYVN VEKEDWKLDT LCDLYETLAI TQSVIFVNTR RKVDWLTDKM 

       310        320        330        340        350        360 
RSRDHTVSAT HGDMDQNTRD IIMREFRSGS SRVLITTDLL ARGIDVQQVS LVINYDLPTQ 

       370        380        390        400        410 
PENYLHRIGR SGRFGRKGVA INFVTRDDER MLFDIQRFYN VTIEELPANV ADLL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of two genes encoding the eukaryotic initiation factor 4A in rice." Kato A., Fujita S., Komeda Y. DNA Seq. 12:295-303(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB046414 mRNA. Translation: BAB21258.1. Frameshift. AB046415 Genomic DNA. Translation: BAB21259.1. Frameshift. AP005288 Genomic DNA. Translation: BAD13081.1. AP008208 Genomic DNA. Translation: BAF07792.1. AK073620 mRNA. No translation available.
RefSeq	NP_001045878.1. NM_001052413.1.
UniGene	Os.458.
3D structure databases
HSSP	HSSP built from PDB template 1FUK based on UniProtKB P10081.
ProteinModelPortal	Q6Z2Z4.
SMR	Q6Z2Z4. Positions 31-414.
ModBase	Search...
Proteomic databases
PaxDb	Q6Z2Z4.
PRIDE	Q6Z2Z4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os02g05330.1; LOC_Os02g05330.1; LOC_Os02g05330.
GeneID	4328286.
KEGG	osa:4328286.
Organism-specific databases
Gramene	Q6Z2Z4.
Phylogenomic databases
eggNOG	COG0513.
HOGENOM	HOG000268797.
KO	K03257.
OMA	SKRERIM.
ProtClustDB	CLSN2679594.
Gene expression databases
ArrayExpress	Q6Z2Z4.
Family and domain databases
InterPro	IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR000629. RNA-helicase_DEAD-box_CS. IPR014014. RNA_helicase_DEAD_Q_motif. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00039. DEAD_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IF4A3_ORYSJ

Accession

Primary (citable) accession number: Q6Z2Z4
Secondary accession number(s): Q9AR32

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Translation initiation factors

List of translation initiation factor entries

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents