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Q6Z2Z4

- IF4A3_ORYSJ

UniProt

Q6Z2Z4 - IF4A3_ORYSJ

Protein

Eukaryotic initiation factor 4A-3

Gene

Os02g0146600

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon By similarity.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi85 – 928ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent helicase activity Source: InterPro
    3. translation initiation factor activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Helicase, Hydrolase, Initiation factor

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Eukaryotic initiation factor 4A-3 (EC:3.6.4.13)
    Short name:
    eIF-4A-3
    Alternative name(s):
    ATP-dependent RNA helicase eIF4A-3
    DEAD-box ATP-dependent RNA helicase 23
    eIF4A-2
    Gene namesi
    Ordered Locus Names:Os02g0146600, LOC_Os02g05330
    ORF Names:OJ1008_C03.10
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 2

    Organism-specific databases

    GrameneiQ6Z2Z4.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 414414Eukaryotic initiation factor 4A-3PRO_0000282441Add
    BLAST

    Proteomic databases

    PaxDbiQ6Z2Z4.
    PRIDEiQ6Z2Z4.

    Interactioni

    Subunit structurei

    eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ6Z2Z4.
    SMRiQ6Z2Z4. Positions 31-414.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini72 – 242171Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini253 – 414162Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi41 – 6929Q motifAdd
    BLAST
    Motifi190 – 1934DEAD box

    Domaini

    The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

    Sequence similaritiesi

    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0513.
    HOGENOMiHOG000268797.
    KOiK03257.
    OMAiCIKGRYD.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000629. RNA-helicase_DEAD-box_CS.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6Z2Z4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGMAPEGSQ FDAKHYDSKM QELLHQGDNE EFFTSYDEVF ESFDDMGLQE    50
    NLLRGIYAYG FEKPSAIQQR GIVPFCKGLD VIQQAQSGTG KTATFCSGIL 100
    QQLDYGLVEC QSLVLAPTRE LAQQIEKVMR ALGDYLGVKV HACVGGTSVR 150
    EDQRILASGV HVVVGTPGRV FDMLRRQSLR PDHIKMFVLD EADEMLSRGF 200
    KDQIYDIFQL LPPKIQVGVF SATMPPEALE ITRKFMNKPV RILVKRDELT 250
    LEGIKQFYVN VEKEDWKLDT LCDLYETLAI TQSVIFVNTR RKVDWLTDKM 300
    RSRDHTVSAT HGDMDQNTRD IIMREFRSGS SRVLITTDLL ARGIDVQQVS 350
    LVINYDLPTQ PENYLHRIGR SGRFGRKGVA INFVTRDDER MLFDIQRFYN 400
    VTIEELPANV ADLL 414
    Length:414
    Mass (Da):47,138
    Last modified:July 5, 2004 - v1
    Checksum:iB440EF6727D791DD
    GO

    Sequence cautioni

    The sequence BAB21258.1 differs from that shown. Reason: Frameshift at positions 31 and 35.
    The sequence BAB21259.1 differs from that shown. Reason: Frameshift at positions 31 and 35.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti38 – 392EV → DL in BAB21258. (PubMed:11913775)Curated
    Sequence conflicti38 – 392EV → DL in BAB21259. (PubMed:11913775)Curated
    Sequence conflicti69 – 691Q → K in BAB21258. (PubMed:11913775)Curated
    Sequence conflicti69 – 691Q → K in BAB21259. (PubMed:11913775)Curated
    Sequence conflicti152 – 1521D → Q in BAB21258. (PubMed:11913775)Curated
    Sequence conflicti152 – 1521D → Q in BAB21259. (PubMed:11913775)Curated
    Sequence conflicti228 – 2281A → G in BAB21258. (PubMed:11913775)Curated
    Sequence conflicti228 – 2281A → G in BAB21259. (PubMed:11913775)Curated
    Sequence conflicti233 – 2342RK → LR in BAB21258. (PubMed:11913775)Curated
    Sequence conflicti233 – 2342RK → LR in BAB21259. (PubMed:11913775)Curated
    Sequence conflicti237 – 2371N → D in BAB21258. (PubMed:11913775)Curated
    Sequence conflicti237 – 2371N → D in BAB21259. (PubMed:11913775)Curated
    Sequence conflicti251 – 2511L → R in BAB21258. (PubMed:11913775)Curated
    Sequence conflicti251 – 2511L → R in BAB21259. (PubMed:11913775)Curated
    Sequence conflicti276 – 2772ET → DS in BAB21258. (PubMed:11913775)Curated
    Sequence conflicti276 – 2772ET → DS in BAB21259. (PubMed:11913775)Curated
    Sequence conflicti290 – 2923RRK → LRM in BAB21258. (PubMed:11913775)Curated
    Sequence conflicti290 – 2923RRK → LRM in BAB21259. (PubMed:11913775)Curated
    Sequence conflicti317 – 3171N → I in BAB21258. (PubMed:11913775)Curated
    Sequence conflicti317 – 3171N → I in BAB21259. (PubMed:11913775)Curated
    Sequence conflicti341 – 3411A → D in BAB21258. (PubMed:11913775)Curated
    Sequence conflicti341 – 3411A → D in BAB21259. (PubMed:11913775)Curated
    Sequence conflicti358 – 3581P → S in BAB21258. (PubMed:11913775)Curated
    Sequence conflicti358 – 3581P → S in BAB21259. (PubMed:11913775)Curated
    Sequence conflicti361 – 3611P → T in BAB21258. (PubMed:11913775)Curated
    Sequence conflicti361 – 3611P → T in BAB21259. (PubMed:11913775)Curated
    Sequence conflicti382 – 3821N → I in BAB21258. (PubMed:11913775)Curated
    Sequence conflicti382 – 3821N → I in BAB21259. (PubMed:11913775)Curated
    Sequence conflicti401 – 4011V → G in BAB21258. (PubMed:11913775)Curated
    Sequence conflicti401 – 4011V → G in BAB21259. (PubMed:11913775)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB046414 mRNA. Translation: BAB21258.1. Frameshift.
    AB046415 Genomic DNA. Translation: BAB21259.1. Frameshift.
    AP005288 Genomic DNA. Translation: BAD13081.1.
    AP008208 Genomic DNA. Translation: BAF07792.1.
    AK073620 mRNA. No translation available.
    RefSeqiNP_001045878.1. NM_001052413.1.
    UniGeneiOs.458.

    Genome annotation databases

    EnsemblPlantsiOS02T0146600-01; OS02T0146600-01; OS02G0146600.
    GeneIDi4328286.
    KEGGiosa:4328286.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB046414 mRNA. Translation: BAB21258.1 . Frameshift.
    AB046415 Genomic DNA. Translation: BAB21259.1 . Frameshift.
    AP005288 Genomic DNA. Translation: BAD13081.1 .
    AP008208 Genomic DNA. Translation: BAF07792.1 .
    AK073620 mRNA. No translation available.
    RefSeqi NP_001045878.1. NM_001052413.1.
    UniGenei Os.458.

    3D structure databases

    ProteinModelPortali Q6Z2Z4.
    SMRi Q6Z2Z4. Positions 31-414.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q6Z2Z4.
    PRIDEi Q6Z2Z4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS02T0146600-01 ; OS02T0146600-01 ; OS02G0146600 .
    GeneIDi 4328286.
    KEGGi osa:4328286.

    Organism-specific databases

    Gramenei Q6Z2Z4.

    Phylogenomic databases

    eggNOGi COG0513.
    HOGENOMi HOG000268797.
    KOi K03257.
    OMAi CIKGRYD.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000629. RNA-helicase_DEAD-box_CS.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00039. DEAD_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of two genes encoding the eukaryotic initiation factor 4A in rice."
      Kato A., Fujita S., Komeda Y.
      DNA Seq. 12:295-303(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Nipponbare.

    Entry informationi

    Entry nameiIF4A3_ORYSJ
    AccessioniPrimary (citable) accession number: Q6Z2Z4
    Secondary accession number(s): Q9AR32
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Translation initiation factors
      List of translation initiation factor entries
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3