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Q6Z2Z4

- IF4A3_ORYSJ

UniProt

Q6Z2Z4 - IF4A3_ORYSJ

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Protein

Eukaryotic initiation factor 4A-3

Gene

Os02g0146600

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi85 – 928ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent helicase activity Source: InterPro
  3. translation initiation factor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

ReactomeiREACT_237162. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
REACT_238412. Ribosomal scanning and start codon recognition.
REACT_248492. ISG15 antiviral mechanism.
REACT_263728. L13a-mediated translational silencing of Ceruloplasmin expression.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic initiation factor 4A-3 (EC:3.6.4.13)
Short name:
eIF-4A-3
Alternative name(s):
ATP-dependent RNA helicase eIF4A-3
DEAD-box ATP-dependent RNA helicase 23
eIF4A-2
Gene namesi
Ordered Locus Names:Os02g0146600, LOC_Os02g05330
ORF Names:OJ1008_C03.10
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 2

Organism-specific databases

GrameneiQ6Z2Z4.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 414414Eukaryotic initiation factor 4A-3PRO_0000282441Add
BLAST

Proteomic databases

PaxDbiQ6Z2Z4.
PRIDEiQ6Z2Z4.

Expressioni

Gene expression databases

ExpressionAtlasiQ6Z2Z4. baseline.

Interactioni

Subunit structurei

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ6Z2Z4.
SMRiQ6Z2Z4. Positions 31-414.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini72 – 242171Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini253 – 414162Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 6929Q motifAdd
BLAST
Motifi190 – 1934DEAD box

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0513.
HOGENOMiHOG000268797.
InParanoidiQ6Z2Z4.
KOiK03257.
OMAiCIKGRYD.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6Z2Z4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGMAPEGSQ FDAKHYDSKM QELLHQGDNE EFFTSYDEVF ESFDDMGLQE
60 70 80 90 100
NLLRGIYAYG FEKPSAIQQR GIVPFCKGLD VIQQAQSGTG KTATFCSGIL
110 120 130 140 150
QQLDYGLVEC QSLVLAPTRE LAQQIEKVMR ALGDYLGVKV HACVGGTSVR
160 170 180 190 200
EDQRILASGV HVVVGTPGRV FDMLRRQSLR PDHIKMFVLD EADEMLSRGF
210 220 230 240 250
KDQIYDIFQL LPPKIQVGVF SATMPPEALE ITRKFMNKPV RILVKRDELT
260 270 280 290 300
LEGIKQFYVN VEKEDWKLDT LCDLYETLAI TQSVIFVNTR RKVDWLTDKM
310 320 330 340 350
RSRDHTVSAT HGDMDQNTRD IIMREFRSGS SRVLITTDLL ARGIDVQQVS
360 370 380 390 400
LVINYDLPTQ PENYLHRIGR SGRFGRKGVA INFVTRDDER MLFDIQRFYN
410
VTIEELPANV ADLL
Length:414
Mass (Da):47,138
Last modified:July 5, 2004 - v1
Checksum:iB440EF6727D791DD
GO

Sequence cautioni

The sequence BAB21258.1 differs from that shown. Reason: Frameshift at positions 31 and 35. Curated
The sequence BAB21259.1 differs from that shown. Reason: Frameshift at positions 31 and 35. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti38 – 392EV → DL in BAB21258. (PubMed:11913775)Curated
Sequence conflicti38 – 392EV → DL in BAB21259. (PubMed:11913775)Curated
Sequence conflicti69 – 691Q → K in BAB21258. (PubMed:11913775)Curated
Sequence conflicti69 – 691Q → K in BAB21259. (PubMed:11913775)Curated
Sequence conflicti152 – 1521D → Q in BAB21258. (PubMed:11913775)Curated
Sequence conflicti152 – 1521D → Q in BAB21259. (PubMed:11913775)Curated
Sequence conflicti228 – 2281A → G in BAB21258. (PubMed:11913775)Curated
Sequence conflicti228 – 2281A → G in BAB21259. (PubMed:11913775)Curated
Sequence conflicti233 – 2342RK → LR in BAB21258. (PubMed:11913775)Curated
Sequence conflicti233 – 2342RK → LR in BAB21259. (PubMed:11913775)Curated
Sequence conflicti237 – 2371N → D in BAB21258. (PubMed:11913775)Curated
Sequence conflicti237 – 2371N → D in BAB21259. (PubMed:11913775)Curated
Sequence conflicti251 – 2511L → R in BAB21258. (PubMed:11913775)Curated
Sequence conflicti251 – 2511L → R in BAB21259. (PubMed:11913775)Curated
Sequence conflicti276 – 2772ET → DS in BAB21258. (PubMed:11913775)Curated
Sequence conflicti276 – 2772ET → DS in BAB21259. (PubMed:11913775)Curated
Sequence conflicti290 – 2923RRK → LRM in BAB21258. (PubMed:11913775)Curated
Sequence conflicti290 – 2923RRK → LRM in BAB21259. (PubMed:11913775)Curated
Sequence conflicti317 – 3171N → I in BAB21258. (PubMed:11913775)Curated
Sequence conflicti317 – 3171N → I in BAB21259. (PubMed:11913775)Curated
Sequence conflicti341 – 3411A → D in BAB21258. (PubMed:11913775)Curated
Sequence conflicti341 – 3411A → D in BAB21259. (PubMed:11913775)Curated
Sequence conflicti358 – 3581P → S in BAB21258. (PubMed:11913775)Curated
Sequence conflicti358 – 3581P → S in BAB21259. (PubMed:11913775)Curated
Sequence conflicti361 – 3611P → T in BAB21258. (PubMed:11913775)Curated
Sequence conflicti361 – 3611P → T in BAB21259. (PubMed:11913775)Curated
Sequence conflicti382 – 3821N → I in BAB21258. (PubMed:11913775)Curated
Sequence conflicti382 – 3821N → I in BAB21259. (PubMed:11913775)Curated
Sequence conflicti401 – 4011V → G in BAB21258. (PubMed:11913775)Curated
Sequence conflicti401 – 4011V → G in BAB21259. (PubMed:11913775)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046414 mRNA. Translation: BAB21258.1. Frameshift.
AB046415 Genomic DNA. Translation: BAB21259.1. Frameshift.
AP005288 Genomic DNA. Translation: BAD13081.1.
AP008208 Genomic DNA. Translation: BAF07792.1.
AK073620 mRNA. No translation available.
RefSeqiNP_001045878.1. NM_001052413.1.
UniGeneiOs.458.

Genome annotation databases

EnsemblPlantsiOS02T0146600-01; OS02T0146600-01; OS02G0146600.
GeneIDi4328286.
KEGGiosa:4328286.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046414 mRNA. Translation: BAB21258.1 . Frameshift.
AB046415 Genomic DNA. Translation: BAB21259.1 . Frameshift.
AP005288 Genomic DNA. Translation: BAD13081.1 .
AP008208 Genomic DNA. Translation: BAF07792.1 .
AK073620 mRNA. No translation available.
RefSeqi NP_001045878.1. NM_001052413.1.
UniGenei Os.458.

3D structure databases

ProteinModelPortali Q6Z2Z4.
SMRi Q6Z2Z4. Positions 31-414.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q6Z2Z4.
PRIDEi Q6Z2Z4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS02T0146600-01 ; OS02T0146600-01 ; OS02G0146600 .
GeneIDi 4328286.
KEGGi osa:4328286.

Organism-specific databases

Gramenei Q6Z2Z4.

Phylogenomic databases

eggNOGi COG0513.
HOGENOMi HOG000268797.
InParanoidi Q6Z2Z4.
KOi K03257.
OMAi CIKGRYD.

Enzyme and pathway databases

Reactomei REACT_237162. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
REACT_238412. Ribosomal scanning and start codon recognition.
REACT_248492. ISG15 antiviral mechanism.
REACT_263728. L13a-mediated translational silencing of Ceruloplasmin expression.

Gene expression databases

ExpressionAtlasi Q6Z2Z4. baseline.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of two genes encoding the eukaryotic initiation factor 4A in rice."
    Kato A., Fujita S., Komeda Y.
    DNA Seq. 12:295-303(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiIF4A3_ORYSJ
AccessioniPrimary (citable) accession number: Q6Z2Z4
Secondary accession number(s): Q9AR32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: July 5, 2004
Last modified: November 26, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3