Reviewed, UniProtKB/Swiss-Prot Q6Z2L5 (KPRS1_ORYSJ)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase 1
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase 1

Gene names

Ordered Locus Names:	Os02g0127700, LOC_Os02g03540
ORF Names:	P0482F12.28-1, P0482F12.28-2, P0576F08.1-1, P0576F08.1-2

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6Z2L5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6Z2L5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     194-224: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Ribose-phosphate pyrophosphokinase 1		PRO_0000141097
Regions
	Region	290 – 305	16	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	204	1	Magnesium By similarity
	Metal binding	206	1	Magnesium By similarity
	Metal binding	215	1	Magnesium By similarity
	Metal binding	219	1	Magnesium By similarity
Natural variations
	Alternative sequence	194 – 224	31	Missing in isoform 2.		VSP_013703

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 10, 2005. Version 2. Checksum: C9B788A6A8DD341A	FASTA	396	42,926
	10 20 30 40 50 60 MPLSYSAAAA AAPSPLAARS RGLLRRPPRS SPVVVRCKKI DQLRAVNGIP PYAPVSNRSL 70 80 90 100 110 120 LSPVTLPIIR DANIKNDTRL RIFSGTANPS LSQEIASYLG LELGKINIKR FADGEIYVQL 130 140 150 160 170 180 QESVRGCDVF LVQPTCPPAN ENLMELLIMI DACRRASAKN ITAVIPYFGY ARADRKSQGR 190 200 210 220 230 240 ESIAAKLVAN MITEAGANRV LVCDLHSSQA MGYFDIPVDH VYGQPVILDY LASKTICSDD 250 260 270 280 290 300 LVVVSPDVGG VARARAFAKK LSDAPLAIVD KRRHGHNVAE VMNLIGDVRG KVAVMMDDMI 310 320 330 340 350 360 DTAGTIAKGA ELLHQEGARE VYACCTHAVF SPPAIERLSS GLFQEVIITN TIPLKEDKSF 370 380 390 PQLTILSVAN LLGETIWRVH DDCSVGHEPY SSLDID
	Isoform 2. Checksum: 7E313A21C7F4D837	FASTA	365	39,551
	10 20 30 40 50 60 MPLSYSAAAA AAPSPLAARS RGLLRRPPRS SPVVVRCKKI DQLRAVNGIP PYAPVSNRSL 70 80 90 100 110 120 LSPVTLPIIR DANIKNDTRL RIFSGTANPS LSQEIASYLG LELGKINIKR FADGEIYVQL 130 140 150 160 170 180 QESVRGCDVF LVQPTCPPAN ENLMELLIMI DACRRASAKN ITAVIPYFGY ARADRKSQGR 190 200 210 220 230 240 ESIAAKLVAN MITPVILDYL ASKTICSDDL VVVSPDVGGV ARARAFAKKL SDAPLAIVDK 250 260 270 280 290 300 RRHGHNVAEV MNLIGDVRGK VAVMMDDMID TAGTIAKGAE LLHQEGAREV YACCTHAVFS 310 320 330 340 350 360 PPAIERLSSG LFQEVIITNT IPLKEDKSFP QLTILSVANL LGETIWRVHD DCSVGHEPYS SLDID

References

[1]	"Oryza sativa nipponbare chromosome 2 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP004886 Genomic DNA. Translation: BAD07971.1. AP004886 Genomic DNA. Translation: BAD07972.1. AP005311 Genomic DNA. Translation: BAD08028.1. AP005311 Genomic DNA. Translation: BAD08029.1. AK060422 mRNA. No translation available. AK072096 mRNA. No translation available. AK099092 mRNA. No translation available.
RefSeq	NP_001045763.1.
UniGene	Os.27658
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4328168.
KEGG	osa:4328168.
Organism-specific databases
Gramene	Q6Z2L5.
Family and domain databases
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS1_ORYSJ

Accession

Primary (citable) accession number: Q6Z2L5
Secondary accession number(s): Q6Z2L6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	May 10, 2005
Last modified:	September 22, 2009
This is version 32 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)