ID   GUN6_ORYSJ              Reviewed;         508 AA.
AC   Q6Z2J3; A0A0N7KG18; Q0DXU1;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   24-JAN-2024, entry version 108.
DE   RecName: Full=Endoglucanase 6;
DE            EC=3.2.1.4;
DE   AltName: Full=Endo-1,4-beta glucanase 6;
DE   AltName: Full=OsCel9E;
DE   Flags: Precursor;
GN   OrderedLocusNames=Os02g0733300, LOC_Os02g50040; ORFNames=P0643A10.32;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [5]
RP   INDUCTION.
RX   PubMed=17056619; DOI=10.1093/pcp/pcl021;
RA   Yoshida K., Imaizumi N., Kaneko S., Kawagoe Y., Tagiri A., Tanaka H.,
RA   Nishitani K., Komae K.;
RT   "Carbohydrate-binding module of a rice endo-beta-1,4-glycanase, OsCel9A,
RT   expressed in auxin-induced lateral root primordia, is post-translationally
RT   truncated.";
RL   Plant Cell Physiol. 47:1555-1571(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- INDUCTION: By auxin in roots. {ECO:0000269|PubMed:17056619}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR   EMBL; AP005319; BAD16147.1; -; Genomic_DNA.
DR   EMBL; AP008208; BAF09947.1; -; Genomic_DNA.
DR   EMBL; AP014958; BAS80777.1; -; Genomic_DNA.
DR   EMBL; AK101108; BAG94917.1; -; mRNA.
DR   EMBL; AK105446; BAG97254.1; -; mRNA.
DR   RefSeq; XP_015625116.1; XM_015769630.1.
DR   AlphaFoldDB; Q6Z2J3; -.
DR   SMR; Q6Z2J3; -.
DR   STRING; 39947.Q6Z2J3; -.
DR   CAZy; GH9; Glycoside Hydrolase Family 9.
DR   PaxDb; 39947-Q6Z2J3; -.
DR   EnsemblPlants; Os02t0733300-01; Os02t0733300-01; Os02g0733300.
DR   GeneID; 4330637; -.
DR   Gramene; Os02t0733300-01; Os02t0733300-01; Os02g0733300.
DR   KEGG; osa:4330637; -.
DR   eggNOG; ENOG502QR9R; Eukaryota.
DR   HOGENOM; CLU_008926_1_2_1; -.
DR   InParanoid; Q6Z2J3; -.
DR   OMA; YLSHANH; -.
DR   OrthoDB; 1347382at2759; -.
DR   Proteomes; UP000000763; Chromosome 2.
DR   Proteomes; UP000059680; Chromosome 2.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR   InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR   PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR   PANTHER; PTHR22298:SF28; ENDOGLUCANASE 8; 1.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR   PROSITE; PS60032; GH9_1; 1.
DR   PROSITE; PS00592; GH9_2; 1.
DR   PROSITE; PS00698; GH9_3; 1.
DR   Genevisible; Q6Z2J3; OS.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW   Cellulose degradation; Glycosidase; Hydrolase; Polysaccharide degradation;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..33
FT                   /evidence="ECO:0000255"
FT   CHAIN           34..508
FT                   /note="Endoglucanase 6"
FT                   /id="PRO_0000249283"
FT   ACT_SITE        89
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT   ACT_SITE        419
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        471
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   ACT_SITE        480
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
SQ   SEQUENCE   508 AA;  55331 MW;  124DF097CB76241C CRC64;
     MLAASLRVEA VAVVAAAVLV LLLSPAAVVV VAGQHDYGDA LHKSILFFEG QRSGRLPPDQ
     RLRWRRDSGL HDGAAASVDL TGGYYDAGDN VKFGFPMAFT ATLMSWGLID FGRSFGPHKE
     EARKAVRWAT DYLMKATAKP NTVYVQVGDA FRDHSCWERP EDMDTPRTVY KVDPSHPGSD
     VAAETAAALA AGSIVFRDAD PAYSKRLLDR AIAVFEFADK YRGPYSSSLH DAVCPCYCDF
     SGYKDELLWG AAWLHKASRR REYREYIKKN EVVLGASESI NEFGWDNKHA GINVLISKEV
     LMGKDEYFQS FRVNADNFMC SLLPGISNHP QIQYSPGGLL FKVGGSNMQH VTSLSFLLLA
     YSNYLSHAGA RVSCGAGGSA SPTQLRRVAK RQVDYILGDN PLRMSYMVGY GARFPRRIHH
     RGSSLPSVAA HPARIGCKGG AAYYASAAPN PNLLVGAVVG GPSDATDAFP DARAVFQQSE
     PTTYINAPLM GLLAYFSAHP NPAEWADD
//