Q6Z2J3 (GUN6_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 6
EC=3.2.1.4

Alternative name(s):
Endo-1,4-beta glucanase 6
OsCel9E

Gene names

Ordered Locus Names:	Os02g0733300, LOC_Os02g50040
ORF Names:	P0643A10.32

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Induction	By auxin in roots. Ref.4
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell wall modification involved in multidimensional cell growth Inferred from electronic annotation. Source: EnsemblPlants/Gramene cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast Inferred from electronic annotation. Source: EnsemblPlants/Gramene extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Potential
	Chain	34 – 508	475	Endoglucanase 6		PRO_0000249283
Sites
	Active site	419	1	By similarity
	Active site	471	1	By similarity
	Active site	480	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6Z2J3 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 124DF097CB76241C	FASTA	508	55,331
	10 20 30 40 50 60 MLAASLRVEA VAVVAAAVLV LLLSPAAVVV VAGQHDYGDA LHKSILFFEG QRSGRLPPDQ 70 80 90 100 110 120 RLRWRRDSGL HDGAAASVDL TGGYYDAGDN VKFGFPMAFT ATLMSWGLID FGRSFGPHKE 130 140 150 160 170 180 EARKAVRWAT DYLMKATAKP NTVYVQVGDA FRDHSCWERP EDMDTPRTVY KVDPSHPGSD 190 200 210 220 230 240 VAAETAAALA AGSIVFRDAD PAYSKRLLDR AIAVFEFADK YRGPYSSSLH DAVCPCYCDF 250 260 270 280 290 300 SGYKDELLWG AAWLHKASRR REYREYIKKN EVVLGASESI NEFGWDNKHA GINVLISKEV 310 320 330 340 350 360 LMGKDEYFQS FRVNADNFMC SLLPGISNHP QIQYSPGGLL FKVGGSNMQH VTSLSFLLLA 370 380 390 400 410 420 YSNYLSHAGA RVSCGAGGSA SPTQLRRVAK RQVDYILGDN PLRMSYMVGY GARFPRRIHH 430 440 450 460 470 480 RGSSLPSVAA HPARIGCKGG AAYYASAAPN PNLLVGAVVG GPSDATDAFP DARAVFQQSE 490 500 PTTYINAPLM GLLAYFSAHP NPAEWADD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[4]	"Carbohydrate-binding module of a rice endo-beta-1,4-glycanase, OsCel9A, expressed in auxin-induced lateral root primordia, is post-translationally truncated." Yoshida K., Imaizumi N., Kaneko S., Kawagoe Y., Tagiri A., Tanaka H., Nishitani K., Komae K. Plant Cell Physiol. 47:1555-1571(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP005319 Genomic DNA. Translation: BAD16147.1. AP008208 Genomic DNA. Translation: BAF09947.1. AK101108 mRNA. Translation: BAG94917.1. AK105446 mRNA. Translation: BAG97254.1.
RefSeq	NP_001048033.1. NM_001054568.1.
UniGene	Os.53973.
3D structure databases
HSSP	HSSP built from PDB template 1TF4 based on UniProtKB P26221.
ProteinModelPortal	Q6Z2J3.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os02g50040.1; LOC_Os02g50040.1; LOC_Os02g50040.
GeneID	4330637.
KEGG	osa:4330637.
Organism-specific databases
Gramene	Q6Z2J3.
Phylogenomic databases
eggNOG	NOG128807.
HOGENOM	HOG000021033.
OMA	INAPLMG.
ProtClustDB	PLN02308.
Gene expression databases
ArrayExpress	Q6Z2J3.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN6_ORYSJ

Accession

Primary (citable) accession number: Q6Z2J3
Secondary accession number(s): Q0DXU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program