Enoyl-[acyl-carrier-protein] reductase [NADH] 1, chloroplastic precursor

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Q6Z0I4 (FABI1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 69. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] 1, chloroplastic
Short name=ENR
EC=1.3.1.9

Alternative name(s):
NADH-dependent enoyl-ACP reductase

Gene names

Ordered Locus Names:	Os08g0327400, LOC_Os08g23810
ORF Names:	OSJNBa0049I01.2, P0670E08.13

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza ›

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the NAD-dependent reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Catalyzes the last reduction step in the de novo synthesis cycle of fatty acids. Involved in the elongation cycle of fatty acids which are used in lipid metabolism. Required for normal plant growth By similarity.
Catalytic activity	Acyl-[acyl-carrier-protein] + NAD⁺ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subunit structure	Homotetramer By similarity.
Subcellular location	Plastid › chloroplast Potential.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	chloroplast envelope Inferred from electronic annotation. Source: EnsemblPlants/Gramene chloroplast stroma Inferred from electronic annotation. Source: EnsemblPlants/Gramene thylakoid Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	copper ion binding Inferred from electronic annotation. Source: EnsemblPlants/Gramene enoyl-[acyl-carrier-protein] reductase (NADH) activity Inferred from electronic annotation. Source: EC enoyl-[acyl-carrier-protein] reductase activity Inferred from electronic annotation. Source: EnsemblPlants/Gramene nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 67

Chloroplast Potential

Chain

68 – 375

308

Enoyl-[acyl-carrier-protein] reductase [NADH] 1, chloroplastic

PRO_0000420279

Regions

Nucleotide binding

155 – 156

NAD By similarity

Nucleotide binding

202 – 203

NAD By similarity

Nucleotide binding

302 – 306

NAD By similarity

Sites

Active site

254

Proton acceptor By similarity

Active site

264

Proton acceptor By similarity

Binding site

NAD; via carbonyl oxygen By similarity

Binding site

NAD By similarity

Binding site

252

NAD; via carbonyl oxygen By similarity

Binding site

272

NAD By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q6Z0I4 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 9D3F6A953D1AAFB1
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FASTA

375

39,131

        10         20         30         40         50         60 
MGASAATGMQ MVAARPCISA SQGMLTSRAA VSRIGRALST TTGFATCPRI CYSSPLGSSK 

        70         80         90        100        110        120 
RSGVAIRAMS SESGPQGLPI DLRGKRAFIA GVADDNGYGW AIAKALAAAG AEILVGTWVP 

       130        140        150        160        170        180 
ALNIFETSLR RGKFDESRKL PDGSLMEIVK VYPLDAVYDS PEDVPEDVKG NKRYAGSSNW 

       190        200        210        220        230        240 
TVKEVAESVK NDFGSIDILV HSLANGPEVT KPLLETSRRG YLAALSASSY SFVSLLQHFL 

       250        260        270        280        290        300 
PIMNPGGASI SLTYIASERA IPGYGGGMSS AKAALESDTK VLAFEAGRKG KIRVNTISAG 

       310        320        330        340        350        360 
PLGSRAAKAI GFIEKMIEYS YVNAPLQKEL LADEVGNTAA FLVSPLASAI TGSTVYVDNG 

       370 
LNTMGLAVDS PTISS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP004759 Genomic DNA. Translation: BAD03449.1. AP005490 Genomic DNA. Translation: BAD03622.1. AP008214 Genomic DNA. Translation: BAF23471.1. AK070992 mRNA. Translation: BAG92250.1.
RefSeq	NP_001061557.1. NM_001068092.1.
UniGene	Os.2695.
3D structure databases
ProteinModelPortal	Q6Z0I4.
ModBase	Search...
Protein-protein interaction databases
STRING	39947.LOC_Os08g23810.1.
Proteomic databases
PRIDE	Q6Z0I4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os08g23810.1; LOC_Os08g23810.1; LOC_Os08g23810. LOC_Os08g23810.2; LOC_Os08g23810.2; LOC_Os08g23810.
GeneID	4345286.
KEGG	osa:4345286.
Organism-specific databases
Gramene	Q6Z0I4.
Phylogenomic databases
KO	K00208.
OMA	DQSIAWG.
ProtClustDB	PLN02730.
Enzyme and pathway databases
UniPathway	UPA00094.
Gene expression databases
ArrayExpress	Q6Z0I4.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
PRINTS	PR00081. GDHRDH.
PROSITE	PS00061. ADH_SHORT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FABI1_ORYSJ

Accession

Primary (citable) accession number: Q6Z0I4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2012
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents