Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q6Z0I4 (FABI1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] 1, chloroplastic

Short name=ENR
EC=1.3.1.9
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene names
Ordered Locus Names:Os08g0327400, LOC_Os08g23810
ORF Names:OSJNBa0049I01.2, P0670E08.13
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length375 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the NAD-dependent reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Catalyzes the last reduction step in the de novo synthesis cycle of fatty acids. Involved in the elongation cycle of fatty acids which are used in lipid metabolism. Required for normal plant growth By similarity.

Catalytic activity

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathway

Lipid metabolism; fatty acid biosynthesis.

Subunit structure

Homotetramer By similarity.

Subcellular location

Plastidchloroplast Potential.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.

Ontologies

Keywords
   Biological processFatty acid biosynthesis
Fatty acid metabolism
Lipid biosynthesis
Lipid metabolism
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processfatty acid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionenoyl-[acyl-carrier-protein] reductase (NADH) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 6767Chloroplast Potential
Chain68 – 375308Enoyl-[acyl-carrier-protein] reductase [NADH] 1, chloroplastic
PRO_0000420279

Regions

Nucleotide binding155 – 1562NAD By similarity
Nucleotide binding202 – 2032NAD By similarity
Nucleotide binding302 – 3065NAD By similarity

Sites

Active site2541Proton acceptor By similarity
Active site2641Proton acceptor By similarity
Binding site911NAD; via carbonyl oxygen By similarity
Binding site981NAD By similarity
Binding site2521NAD; via carbonyl oxygen By similarity
Binding site2721NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6Z0I4 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 9D3F6A953D1AAFB1

FASTA37539,131
        10         20         30         40         50         60 
MGASAATGMQ MVAARPCISA SQGMLTSRAA VSRIGRALST TTGFATCPRI CYSSPLGSSK 

        70         80         90        100        110        120 
RSGVAIRAMS SESGPQGLPI DLRGKRAFIA GVADDNGYGW AIAKALAAAG AEILVGTWVP 

       130        140        150        160        170        180 
ALNIFETSLR RGKFDESRKL PDGSLMEIVK VYPLDAVYDS PEDVPEDVKG NKRYAGSSNW 

       190        200        210        220        230        240 
TVKEVAESVK NDFGSIDILV HSLANGPEVT KPLLETSRRG YLAALSASSY SFVSLLQHFL 

       250        260        270        280        290        300 
PIMNPGGASI SLTYIASERA IPGYGGGMSS AKAALESDTK VLAFEAGRKG KIRVNTISAG 

       310        320        330        340        350        360 
PLGSRAAKAI GFIEKMIEYS YVNAPLQKEL LADEVGNTAA FLVSPLASAI TGSTVYVDNG 

       370 
LNTMGLAVDS PTISS 

« Hide

References

« Hide 'large scale' references
[1]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[3]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP004759 Genomic DNA. Translation: BAD03449.1.
AP005490 Genomic DNA. Translation: BAD03622.1.
AP008214 Genomic DNA. Translation: BAF23471.1.
AK070992 mRNA. Translation: BAG92250.1.
RefSeqNP_001061557.1. NM_001068092.1.
UniGeneOs.2695.

3D structure databases

ProteinModelPortalQ6Z0I4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING39947.LOC_Os08g23810.1.

Proteomic databases

PRIDEQ6Z0I4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS08T0327400-01; OS08T0327400-01; OS08G0327400.
GeneID4345286.
KEGGosa:4345286.

Organism-specific databases

GrameneQ6Z0I4.

Phylogenomic databases

KOK00208.
OMAHEVLNDK.

Enzyme and pathway databases

UniPathwayUPA00094.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PRINTSPR00081. GDHRDH.
ProtoNetSearch...

Entry information

Entry nameFABI1_ORYSJ
AccessionPrimary (citable) accession number: Q6Z0I4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways