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Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] 1, chloroplastic

Gene

Os08g0327400

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the NAD-dependent reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Catalyzes the last reduction step in the de novo synthesis cycle of fatty acids. Involved in the elongation cycle of fatty acids which are used in lipid metabolism. Required for normal plant growth (By similarity).By similarity

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei91 – 911NAD; via carbonyl oxygenBy similarity
Binding sitei98 – 981NADBy similarity
Binding sitei252 – 2521NAD; via carbonyl oxygenBy similarity
Active sitei254 – 2541Proton acceptorBy similarity
Active sitei264 – 2641Proton acceptorBy similarity
Binding sitei272 – 2721NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi155 – 1562NADBy similarity
Nucleotide bindingi202 – 2032NADBy similarity
Nucleotide bindingi302 – 3065NADBy similarity

GO - Molecular functioni

  1. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB-EC

GO - Biological processi

  1. fatty acid biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] 1, chloroplastic (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Ordered Locus Names:Os08g0327400, LOC_Os08g23810
ORF Names:OSJNBa0049I01.2, P0670E08.13
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763 Componenti: Chromosome 8

Organism-specific databases

GrameneiQ6Z0I4.

Subcellular locationi

Plastidchloroplast Curated

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6767ChloroplastSequence AnalysisAdd
BLAST
Chaini68 – 375308Enoyl-[acyl-carrier-protein] reductase [NADH] 1, chloroplasticPRO_0000420279Add
BLAST

Proteomic databases

PRIDEiQ6Z0I4.

Expressioni

Gene expression databases

ExpressionAtlasiQ6Z0I4. baseline.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi39947.LOC_Os08g23810.1.

Structurei

3D structure databases

ProteinModelPortaliQ6Z0I4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

InParanoidiQ6Z0I4.
KOiK00208.
OMAiATCPRIC.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PRINTSiPR00081. GDHRDH.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6Z0I4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGASAATGMQ MVAARPCISA SQGMLTSRAA VSRIGRALST TTGFATCPRI
60 70 80 90 100
CYSSPLGSSK RSGVAIRAMS SESGPQGLPI DLRGKRAFIA GVADDNGYGW
110 120 130 140 150
AIAKALAAAG AEILVGTWVP ALNIFETSLR RGKFDESRKL PDGSLMEIVK
160 170 180 190 200
VYPLDAVYDS PEDVPEDVKG NKRYAGSSNW TVKEVAESVK NDFGSIDILV
210 220 230 240 250
HSLANGPEVT KPLLETSRRG YLAALSASSY SFVSLLQHFL PIMNPGGASI
260 270 280 290 300
SLTYIASERA IPGYGGGMSS AKAALESDTK VLAFEAGRKG KIRVNTISAG
310 320 330 340 350
PLGSRAAKAI GFIEKMIEYS YVNAPLQKEL LADEVGNTAA FLVSPLASAI
360 370
TGSTVYVDNG LNTMGLAVDS PTISS
Length:375
Mass (Da):39,131
Last modified:July 4, 2004 - v1
Checksum:i9D3F6A953D1AAFB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP004759 Genomic DNA. Translation: BAD03449.1.
AP005490 Genomic DNA. Translation: BAD03622.1.
AP008214 Genomic DNA. Translation: BAF23471.1.
AK070992 mRNA. Translation: BAG92250.1.
RefSeqiNP_001061557.1. NM_001068092.1.
UniGeneiOs.2695.

Genome annotation databases

EnsemblPlantsiOS08T0327400-01; OS08T0327400-01; OS08G0327400.
GeneIDi4345286.
KEGGiosa:4345286.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP004759 Genomic DNA. Translation: BAD03449.1.
AP005490 Genomic DNA. Translation: BAD03622.1.
AP008214 Genomic DNA. Translation: BAF23471.1.
AK070992 mRNA. Translation: BAG92250.1.
RefSeqiNP_001061557.1. NM_001068092.1.
UniGeneiOs.2695.

3D structure databases

ProteinModelPortaliQ6Z0I4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os08g23810.1.

Proteomic databases

PRIDEiQ6Z0I4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS08T0327400-01; OS08T0327400-01; OS08G0327400.
GeneIDi4345286.
KEGGiosa:4345286.

Organism-specific databases

GrameneiQ6Z0I4.

Phylogenomic databases

InParanoidiQ6Z0I4.
KOiK00208.
OMAiATCPRIC.

Enzyme and pathway databases

UniPathwayiUPA00094.

Gene expression databases

ExpressionAtlasiQ6Z0I4. baseline.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PRINTSiPR00081. GDHRDH.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  3. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiFABI1_ORYSJ
AccessioniPrimary (citable) accession number: Q6Z0I4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 27, 2012
Last sequence update: July 4, 2004
Last modified: January 6, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.