Q6YZX6 (ACOC_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative aconitate hydratase, cytoplasmic
Short name=Aconitase
EC=4.2.1.3

Alternative name(s):
Citrate hydro-lyase

Gene names

Ordered Locus Names:	Os08g0191100, LOC_Os08g09200
ORF Names:	P0610E02.32

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	898 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the isomerization of citrate to isocitrate via cis-aconitate By similarity.
Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Carbohydrate metabolism; glyoxylate and dicarboxylate metabolism.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW aconitate hydratase activity Inferred from electronic annotation. Source: EC citrate hydro-lyase (cis-aconitate-forming) activity Inferred from electronic annotation. Source: EC isocitrate hydro-lyase (cis-aconitate-forming) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 898	898	Putative aconitate hydratase, cytoplasmic		PRO_0000247306
Regions
	Region	209 – 211	3	Substrate binding By similarity
	Region	784 – 785	2	Substrate binding By similarity
Sites
	Metal binding	441	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	507	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	510	1	Iron-sulfur (4Fe-4S) By similarity
	Binding site	90	1	Substrate By similarity
	Binding site	540	1	Substrate By similarity
	Binding site	545	1	Substrate By similarity
	Binding site	703	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6YZX6 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: BB808EB283E695A8	FASTA	898	98,083
	10 20 30 40 50 60 MAAEHPFKNI LTTLPKPGGG EYGKFYSLPA LNDPRIDKLP YSIRILLESA IRNCDNFQVN 70 80 90 100 110 120 QNDVEKIIDW ENTSPKLAEI PFKPARVLLQ DFTGVPAVVD LAAMRDAMAK LGSDANKINP 130 140 150 160 170 180 LVPVDLVIDH SVQVDVARSP NAVQSNMELE FKRNNERFGF LKWGSTAFHN MLVVPPGSGI 190 200 210 220 230 240 VHQVNLEYLG RVVFNTDGIM YPDSVVGTDS HTTMIDGLGV AGWGVGGIEA EATMLGQPMS 250 260 270 280 290 300 MVLPGVVGFK LTGKLQNGVT ATDLVLTVTQ MLRKHGVVGK FVEFYGEGMG KLSLADRATI 310 320 330 340 350 360 ANMSPEYGAT MGFFPVDHVT LDYLKLTGRS DETVAMIEAY LRANKMFVDY NEPQTERVYS 370 380 390 400 410 420 SYLELDLNEV EPCISGPKRP HDRVLLKEMK SDWHSCLDNR VGFKGFAVPK EQQDKVVKFD 430 440 450 460 470 480 FHGQPAELKH GSVVIAAITS CTNTSNPSVM LGAALVAKKA CELGLEVKPW VKTSLAPGSG 490 500 510 520 530 540 VVTKYLLQSG LQEYLNKQGF HVVGYGCTTC IGNSGDLDES VSAAISENDV VAAAVLSGNR 550 560 570 580 590 600 NFEGRVHPLT RANYLASPPL VVAYALAGTV DIDFEKEPIG VGKDGKEVFF RDIWPSTEEI 610 620 630 640 650 660 AEVVQSSVLP DMFKSTYEAI TKGNPMWNQL TVPEASLYSW DPNSTYIHEP PYFKDMTMSP 670 680 690 700 710 720 PGPHGVKNAY CLLNFGDSIT TDHISPAGSI HKDSPAAKYL LERGVDRKDF NSYGSRRGND 730 740 750 760 770 780 EVMARGTFAN IRIVNKFLNG EVGPKTVHVP TGEKLYVFDA ALKYKSEGHD TIVLAGAEYG 790 800 810 820 830 840 SGSSRDWAAK GPMLLGVKAV IAKSFERIHR SNLVGMGIIP LCFKAGEDAD SLGLTGHERY 850 860 870 880 890 TIDLPTNVSE IRPGQDITVT TDNGKSFTCT LRFDTEVELA YFNHGGILPY VIRNLAQN

References

[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP005505 Genomic DNA. Translation: BAD05751.1.
3D structure databases
ProteinModelPortal	Q6YZX6.
SMR	Q6YZX6. Positions 5-897.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6YZX6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	Q6YZX6.
Phylogenomic databases
GeneTree	EPGT00070000028605.
Family and domain databases
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR015937. Acoase/IPM_deHydtase. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR006249. Aconitase/Fe_reg_prot_2. IPR015934. Aconitase/Fe_reg_prot_2/AcnD. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
Gene3D	G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 3 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF1. Aconitase/Fe_reg_prot_2/AcnD. 1 hit.
Pfam	PF00330. Aconitase. 1 hit. PF00694. Aconitase_C. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit. SSF53732. Aconitase_N. 1 hit.
TIGRFAMs	TIGR01341. Aconitase_1. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACOC_ORYSJ

Accession

Primary (citable) accession number: Q6YZX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program