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Protein

Proteasome subunit alpha type-7-A

Gene

Os08g0548900

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiREACT_283342. APC/C:Cdc20 mediated degradation of Securin.
REACT_292166. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_295447. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_300539. Hedgehog 'on' state.
REACT_301524. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_311865. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_319722. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_330023. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_333718. Separation of Sister Chromatids.
REACT_336348. CDK-mediated phosphorylation and removal of Cdc6.
REACT_340130. ER-Phagosome pathway.
REACT_344832. Orc1 removal from chromatin.
REACT_346168. Autodegradation of Cdh1 by Cdh1:APC/C.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-7-A (EC:3.4.25.1)
Alternative name(s):
20S proteasome alpha subunit D-1
20S proteasome subunit alpha-4-A
Gene namesi
Ordered Locus Names:Os08g0548900, LOC_Os08g43540
ORF Names:OJ1112_E06.28, OJ1479_B11.3, OsJ_000765
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763 Componenti: Chromosome 8

Organism-specific databases

GrameneiQ6YT00.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
  3. proteasome core complex, alpha-subunit complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 249249Proteasome subunit alpha type-7-APRO_0000301664Add
BLAST

Proteomic databases

PaxDbiQ6YT00.
PRIDEiQ6YT00.

Expressioni

Gene expression databases

ExpressionAtlasiQ6YT00. baseline.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.

Structurei

3D structure databases

ProteinModelPortaliQ6YT00.
SMRiQ6YT00. Positions 4-231.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0638.
InParanoidiQ6YT00.
KOiK02731.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6YT00-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARYDRAITV FSPDGHLFQV EYALEAVRKG NAAVGVRGTD TVVLGVEKKS
60 70 80 90 100
TPKLQDSRSM RKIASLDTHI ALACAGLKAD ARVLINRARV ECQSHRLTVE
110 120 130 140 150
DPVTVEYITR YIAGLQQKYT QSGGVRPFGL STLIVGFDPY TEKPALYQTD
160 170 180 190 200
PSGTFSAWKA NATGRNSNSM REFLEKNYKD TSGKETIKLA IRALLEVVES
210 220 230 240
GGKNIEIAVM THKDGLRELE EAEIDEYVAE IEAEKAAAEA AKKGAPKET
Length:249
Mass (Da):27,296
Last modified:July 4, 2004 - v1
Checksum:iD6F10209082B4797
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti88 – 881A → V in AK061808 (PubMed:12869764).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003912 Genomic DNA. Translation: BAD08948.1.
AP006265 Genomic DNA. Translation: BAD10760.1.
AP008214 Genomic DNA. Translation: BAF24351.1.
CM000138 Genomic DNA. No translation available.
AK061808 mRNA. No translation available.
RefSeqiNP_001062437.1. NM_001068972.1.
UniGeneiOs.7903.

Genome annotation databases

EnsemblPlantsiOS08T0548900-01; OS08T0548900-01; OS08G0548900.
GeneIDi4346245.
KEGGiosa:4346245.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003912 Genomic DNA. Translation: BAD08948.1.
AP006265 Genomic DNA. Translation: BAD10760.1.
AP008214 Genomic DNA. Translation: BAF24351.1.
CM000138 Genomic DNA. No translation available.
AK061808 mRNA. No translation available.
RefSeqiNP_001062437.1. NM_001068972.1.
UniGeneiOs.7903.

3D structure databases

ProteinModelPortaliQ6YT00.
SMRiQ6YT00. Positions 4-231.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ6YT00.
PRIDEiQ6YT00.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS08T0548900-01; OS08T0548900-01; OS08G0548900.
GeneIDi4346245.
KEGGiosa:4346245.

Organism-specific databases

GrameneiQ6YT00.

Phylogenomic databases

eggNOGiCOG0638.
InParanoidiQ6YT00.
KOiK02731.

Enzyme and pathway databases

ReactomeiREACT_283342. APC/C:Cdc20 mediated degradation of Securin.
REACT_292166. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_295447. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_300539. Hedgehog 'on' state.
REACT_301524. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_311865. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_319722. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_330023. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_333718. Separation of Sister Chromatids.
REACT_336348. CDK-mediated phosphorylation and removal of Cdc6.
REACT_340130. ER-Phagosome pathway.
REACT_344832. Orc1 removal from chromatin.
REACT_346168. Autodegradation of Cdh1 by Cdh1:APC/C.

Gene expression databases

ExpressionAtlasiQ6YT00. baseline.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  3. "The genomes of Oryza sativa: a history of duplications."
    Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
    , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
    PLoS Biol. 3:266-281(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiPSA7A_ORYSJ
AccessioniPrimary (citable) accession number: Q6YT00
Secondary accession number(s): A2ZQE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 10, 2007
Last sequence update: July 4, 2004
Last modified: March 31, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.