Q6YR76 (SYE_ONYPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	PAM_140

Organism

Onion yellows phytoplasma (strain OY-M) [Complete proteome] [HAMAP]

Taxonomic identifier

262768 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Acholeplasmatales › Acholeplasmataceae › Candidatus Phytoplasma › Candidatus Phytoplasma asteris

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000119617
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022_B
	Motif	214 – 218	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	217	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6YR76 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: E3C2C2FD0D9CA4FC	FASTA	449	52,352
	10 20 30 40 50 60 MKKIKVRYAP SPTGFLHIGN ARTALFNYLF AKQNQGEFII RIEDTDFSRN VEGGEASQLK 70 80 90 100 110 120 NLRWLGIDWS EGPDIQGPFG PYRQSERLAI YQKYAQKLLD QDLAYKEFQE GHTTFAIRFR 130 140 150 160 170 180 VPTNQTFAFD DLIRGKLTFQ SQEIEDFIIL KSNGYPSYNF AVVIDDHLMQ ISHIFRGEEH 190 200 210 220 230 240 ITNTPKQIMI YQAFQWHLPQ FAHMTLILNE NKKKLSKRDA NIMQFIEQYE KLGYLPQALF 250 260 270 280 290 300 NFLSLLGFSP LSQTEILSPQ ELINLFDVAR LNKAPAMFDK TKLDYLNNQH LRKLLPEVIA 310 320 330 340 350 360 SFILQKKYLA LTTAPTNYKE WMTKFVALFQ DRMHYMQQIT DFYQLFFQST PFLSQEATHF 370 380 390 400 410 420 LQTNPQTTLI LKTFYNVFDV IVFEKDVIAN SIKQVTTQND FAKKTLFMAL RIGTTCKMHG 430 440 PSIALLLELL GKKQVLKNLS YVLEQAQKF

References

[1]

"Reductive evolution suggested from the complete genome sequence of a plant-pathogenic phytoplasma." Oshima K., Kakizawa S., Nishigawa H., Jung H.-Y., Wei W., Suzuki S., Arashida R., Nakata D., Miyata S., Ugaki M., Namba S. Nat. Genet. 36:27-29(2004) [PubMed: 14661021] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OY-M.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006628 Genomic DNA. Translation: BAD04225.1.
RefSeq	NP_950392.1. NC_005303.2.
3D structure databases
ProteinModelPortal	Q6YR76.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2673455.
GenomeReviews	Gene locus PAM_140 in contig AP006628_GR.
KEGG	poy:PAM_140.
PATRIC	22809738. VBIOniYel86799_0197.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	AHMTLIL.
PhylomeDB	Q6YR76.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	PAST100379:PAM140-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 2 hits. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_ONYPE

Accession

Primary (citable) accession number: Q6YR76

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program