SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q6YR34

- SYI_ONYPE

UniProt

Q6YR34 - SYI_ONYPE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Isoleucine--tRNA ligase
Gene
ileS, PAM_184
Organism
Onion yellows phytoplasma (strain OY-M)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei550 – 5501Aminoacyl-adenylate By similarity
Binding sitei594 – 5941ATP By similarity
Metal bindingi876 – 8761Zinc By similarity
Metal bindingi879 – 8791Zinc By similarity
Metal bindingi892 – 8921Zinc By similarity
Metal bindingi895 – 8951Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMORG262768:GHF5-191-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:PAM_184
OrganismiOnion yellows phytoplasma (strain OY-M)
Taxonomic identifieri262768 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesAcholeplasmatalesAcholeplasmataceaeCandidatus PhytoplasmaCandidatus Phytoplasma asteris
ProteomesiUP000002523: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 900900Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098433Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi262768.PAM_184.

Structurei

3D structure databases

ProteinModelPortaliQ6YR34.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionUniRule annotation
Add
BLAST
Motifi591 – 5955"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKESHFDE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6YR34-1 [UniParc]FASTAAdd to Basket

« Hide

MTTNYKTTLL MPKTDFPMKG NLGKNEINIQ KHWQKLDLYQ KKLQQNQDNN    50
PFILHDGPPY ANGNIHMGHA LNKILKDFIV RFRSMQGFYT PLIPGWDTHG 100
LPIEAAVLKK TSKNAFTRKP LLDKCQEFAL ENVNNQKHQF QRLGILGDWQ 150
NPYLTLDKTF VSDQVRIFGQ MVDKGLIFKA LKPIHWSPTL ESALAEAELE 200
YHNHQSPSVY VAFSMKKLDI FDNVALVIWT TTPWTLPANV AIAVHPEKEY 250
QLIEVLQKRY LVGTKNIPFL QKVFAWNKEN IKVVATFEGK TLEHLTYQNH 300
LVSKFGKIIL SQHVLDGEGT GLVHIAPGHG LDDFLVGQKY NLDVVCSIDK 350
KGMMTDVSKY QGLFYTKANE AIISDLEKDH SLLKADVILH SYPHDWRTKK 400
PVISLALPQW FVSIKKIKSL LLEETQKVKW IPRWGELKMT NMITNREDWN 450
ISRQRTWGVP IPIFYTETHQ PILDLKLINH VADLFEQHGM DIWYEWDVKK 500
LLPENYINPQ SPNNLFTKEL DIMDVWFDSG TSYSVFKKRN QVLQSDVYLE 550
GSDQYRGWFN SSLITSVATQ NQAPYKTVIT HGFVFDGEGK KMSKSLGNVI 600
DPLTVAEQKG ADIIRLWVAN TNYNLDVRIN PSILKQVEDL YRKIRNTFRF 650
MLGNLDNFKK DTNYIAFEQR TFIHQAMMLD FEEVLKNVLH SYDTYNFEGV 700
LRHLFPFITN KISAFYLDFA KDILYIEKED HKERKMIQST IYDLLLSLLQ 750
VLTPIIPHTT SEVYGFFPFA VEKDIYLEKM PQLKARPTSH LLLEYHKFLT 800
LRKNVLQYLE KARQSGLINS SLQAHITLSL TQEEMHALDV LQIKDQLHQL 850
FIVSKVTLQL KDTFDVKVAK ASGYACQRCW NVVITKPLTP LCTRCQNILK 900
Length:900
Mass (Da):104,373
Last modified:July 5, 2004 - v1
Checksum:i023BA5BF424BF031
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006628 Genomic DNA. Translation: BAD04269.1.
RefSeqiNP_950436.1. NC_005303.2.

Genome annotation databases

EnsemblBacteriaiBAD04269; BAD04269; PAM_184.
GeneIDi2673552.
KEGGipoy:PAM_184.
PATRICi22809860. VBIOniYel86799_0253.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006628 Genomic DNA. Translation: BAD04269.1 .
RefSeqi NP_950436.1. NC_005303.2.

3D structure databases

ProteinModelPortali Q6YR34.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 262768.PAM_184.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD04269 ; BAD04269 ; PAM_184 .
GeneIDi 2673552.
KEGGi poy:PAM_184.
PATRICi 22809860. VBIOniYel86799_0253.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KESHFDE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci MORG262768:GHF5-191-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Reductive evolution suggested from the complete genome sequence of a plant-pathogenic phytoplasma."
    Oshima K., Kakizawa S., Nishigawa H., Jung H.-Y., Wei W., Suzuki S., Arashida R., Nakata D., Miyata S., Ugaki M., Namba S.
    Nat. Genet. 36:27-29(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: OY-M.

Entry informationi

Entry nameiSYI_ONYPE
AccessioniPrimary (citable) accession number: Q6YR34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi