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Protein

G-protein coupled receptor 6

Gene

Gpr6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Orphan receptor with constitutive G(s) signaling activity that activate cyclic AMP. Promotes neurite outgrowth and blocks myelin inhibition in neurons (By similarity).By similarity

GO - Molecular functioni

  • sphingosine-1-phosphate receptor activity Source: MGI

GO - Biological processi

  • positive regulation of cytosolic calcium ion concentration Source: MGI
  • sphingosine-1-phosphate signaling pathway Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein coupled receptor 6
Alternative name(s):
Sphingosine 1-phosphate receptor GPR6
Gene namesi
Name:Gpr6
Synonyms:Gm233
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2155249. Gpr6.

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

  • Note: Highly expressed and localized along the cytoplasmic membrane as well as in a perinuclear compartment.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7575ExtracellularSequence AnalysisAdd
BLAST
Transmembranei76 – 9520Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini96 – 10712CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei108 – 13124Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini132 – 14312ExtracellularSequence AnalysisAdd
BLAST
Transmembranei144 – 16522Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini166 – 18621CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei187 – 20620Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini207 – 23125ExtracellularSequence AnalysisAdd
BLAST
Transmembranei232 – 25019Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini251 – 27828CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei279 – 30527Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini306 – 3105ExtracellularSequence Analysis
Transmembranei311 – 33222Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini333 – 36331CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • cytosol Source: GOC
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deficient mice show reduced striatal cyclic AMP production and selective alterations in instrumental conditioning.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 363363G-protein coupled receptor 6PRO_0000069515Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi2 – 21N-linked (GlcNAc...)Sequence Analysis
Glycosylationi9 – 91N-linked (GlcNAc...)Sequence Analysis
Glycosylationi52 – 521N-linked (GlcNAc...)Sequence Analysis
Lipidationi346 – 3461S-palmitoyl cysteineBy similarity
Modified residuei357 – 3571PhosphoserineSequence Analysis
Modified residuei359 – 3591PhosphoserineSequence Analysis
Modified residuei361 – 3611PhosphoserineSequence Analysis

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PRIDEiQ6YNI2.

Expressioni

Tissue specificityi

Mainly expressed in the brain. Selectively expressed in striatopallidal neurons in the striatum.2 Publications

Gene expression databases

BgeeiQ6YNI2.
CleanExiMM_GPR6.
ExpressionAtlasiQ6YNI2. baseline and differential.
GenevisibleiQ6YNI2. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000057323.

Structurei

3D structure databases

ProteinModelPortaliQ6YNI2.
SMRiQ6YNI2. Positions 78-346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi21 – 288Poly-Ala
Compositional biasi46 – 505Poly-Gly

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG327877.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000294075.
HOVERGENiHBG079561.
InParanoidiQ6YNI2.
KOiK04313.
OMAiCGCFQSK.
OrthoDBiEOG7966H0.
PhylomeDBiQ6YNI2.
TreeFamiTF330052.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001151. GPR6.
IPR000723. GPR_3/6/12_orphan.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00649. GPR6ORPHANR.
PR00644. GPRORPHANR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6YNI2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNASAAALNE SQVVAVAAEG AAAAATAAGA PDTGEWGPPA ASAALGGGGG
60 70 80 90 100
PNGSLELSSQ LPAGPSGLLL SAVNPWDVLL CVSGTVIAGE NALVVALIAS
110 120 130 140 150
TPALRTPMFV LVGSLATADL LAGCGLILHF VFQYVVPSET VSLLMVGFLV
160 170 180 190 200
ASFAASVSSL LAITVDRYLS LYNALTYYSR RTLLGVHLLL AATWTVSLGL
210 220 230 240 250
GLLPVLGWNC LADRTSCSVV RPLTRSHVAL LSTSFFVVFG IMLHLYVRIC
260 270 280 290 300
QVVWRHAHQI ALQQHCLAPP HLAATRKGVG TLAVVLGTFG ASWLPFAIYC
310 320 330 340 350
VVGSQEDPAI YTYATLLPAT YNSMINPIIY AFRNQEIQRA LWLLFCGCFQ
360
SKVPFRSRSP SEV
Length:363
Mass (Da):38,085
Last modified:July 5, 2004 - v1
Checksum:i6870FF769BAAF093
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY064488 mRNA. Translation: AAL40876.1.
AK139367 mRNA. Translation: BAE23977.1.
CCDSiCCDS23802.1.
RefSeqiNP_951013.1. NM_199058.1.
XP_006512596.1. XM_006512533.2.
UniGeneiMm.290693.

Genome annotation databases

EnsembliENSMUST00000061796; ENSMUSP00000057323; ENSMUSG00000046922.
GeneIDi140741.
KEGGimmu:140741.
UCSCiuc007exj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY064488 mRNA. Translation: AAL40876.1.
AK139367 mRNA. Translation: BAE23977.1.
CCDSiCCDS23802.1.
RefSeqiNP_951013.1. NM_199058.1.
XP_006512596.1. XM_006512533.2.
UniGeneiMm.290693.

3D structure databases

ProteinModelPortaliQ6YNI2.
SMRiQ6YNI2. Positions 78-346.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000057323.

Chemistry

GuidetoPHARMACOLOGYi85.

Protein family/group databases

GPCRDBiSearch...

Proteomic databases

PRIDEiQ6YNI2.

Protocols and materials databases

DNASUi140741.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000061796; ENSMUSP00000057323; ENSMUSG00000046922.
GeneIDi140741.
KEGGimmu:140741.
UCSCiuc007exj.1. mouse.

Organism-specific databases

CTDi2830.
MGIiMGI:2155249. Gpr6.

Phylogenomic databases

eggNOGiNOG327877.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000294075.
HOVERGENiHBG079561.
InParanoidiQ6YNI2.
KOiK04313.
OMAiCGCFQSK.
OrthoDBiEOG7966H0.
PhylomeDBiQ6YNI2.
TreeFamiTF330052.

Miscellaneous databases

NextBioi369959.
PROiQ6YNI2.
SOURCEiSearch...

Gene expression databases

BgeeiQ6YNI2.
CleanExiMM_GPR6.
ExpressionAtlasiQ6YNI2. baseline and differential.
GenevisibleiQ6YNI2. MM.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001151. GPR6.
IPR000723. GPR_3/6/12_orphan.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00649. GPR6ORPHANR.
PR00644. GPRORPHANR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sphingosine-1-phosphate is a high-affinity ligand for the G protein-coupled receptor GPR6 from mouse and induces intracellular Ca2+ release by activating the sphingosine-kinase pathway."
    Ignatov A., Lintzel J., Kreienkamp H.J., Schaller H.C.
    Biochem. Biophys. Res. Commun. 311:329-336(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: C57BL/6.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain cortex.
  3. "Neural expression of G protein-coupled receptors GPR3, GPR6, and GPR12 up-regulates cyclic AMP levels and promotes neurite outgrowth."
    Tanaka S., Ishii K., Kasai K., Yoon S.O., Saeki Y.
    J. Biol. Chem. 282:10506-10515(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  4. "Genetic control of instrumental conditioning by striatopallidal neuron-specific S1P receptor Gpr6."
    Lobo M.K., Cui Y., Ostlund S.B., Balleine B.W., Yang X.W.
    Nat. Neurosci. 10:1395-1397(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiGPR6_MOUSE
AccessioniPrimary (citable) accession number: Q6YNI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: July 5, 2004
Last modified: June 24, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally (PubMed:14592418) thought to be a receptor for sphingosine 1-phosphate. It has been demonstrated that it is not the case in human.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.