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Protein

Hydroxysteroid dehydrogenase-like protein 2

Gene

HSDL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has apparently no steroid dehydrogenase activity.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421NADP1 Publication
Binding sitei74 – 741NADP1 Publication
Active sitei168 – 1681Proton acceptorPROSITE-ProRule annotation
Binding sitei172 – 1721NADP1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 237NADP1 Publication

GO - Molecular functioni

  1. oxidoreductase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxysteroid dehydrogenase-like protein 2 (EC:1.-.-.-)
Gene namesi
Name:HSDL2
Synonyms:C9orf99
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 9

Organism-specific databases

HGNCiHGNC:18572. HSDL2.

Subcellular locationi

Peroxisome 1 Publication

GO - Cellular componenti

  1. membrane Source: UniProtKB
  2. mitochondrion Source: Ensembl
  3. peroxisome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134980105.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 418418Hydroxysteroid dehydrogenase-like protein 2PRO_0000319888Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei116 – 1161N6-acetyllysineBy similarity
Modified residuei318 – 3181N6-succinyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ6YN16.
PaxDbiQ6YN16.
PRIDEiQ6YN16.

2D gel databases

UCD-2DPAGEQ6YN16.

PTM databases

PhosphoSiteiQ6YN16.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiQ6YN16.
CleanExiHS_HSDL2.
ExpressionAtlasiQ6YN16. baseline and differential.
GenevestigatoriQ6YN16.

Organism-specific databases

HPAiHPA042890.
HPA050453.

Interactioni

Protein-protein interaction databases

BioGridi123990. 6 interactions.
IntActiQ6YN16. 2 interactions.
STRINGi9606.ENSP00000381785.

Structurei

Secondary structure

1
418
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni7 – 104Combined sources
Beta strandi12 – 165Combined sources
Turni17 – 193Combined sources
Helixi21 – 3111Combined sources
Turni32 – 343Combined sources
Beta strandi36 – 427Combined sources
Beta strandi48 – 503Combined sources
Helixi54 – 6310Combined sources
Beta strandi67 – 726Combined sources
Helixi78 – 9215Combined sources
Beta strandi97 – 1004Combined sources
Turni110 – 1123Combined sources
Helixi115 – 12410Combined sources
Helixi126 – 14015Combined sources
Beta strandi147 – 1515Combined sources
Helixi159 – 1613Combined sources
Beta strandi163 – 1653Combined sources
Helixi166 – 18520Combined sources
Turni186 – 1894Combined sources
Beta strandi191 – 1966Combined sources
Helixi204 – 2096Combined sources
Helixi215 – 2173Combined sources
Helixi222 – 23211Combined sources
Beta strandi242 – 2443Combined sources
Helixi245 – 2517Combined sources
Helixi257 – 2604Combined sources
Beta strandi261 – 2633Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3KVOX-ray2.25A/B1-293[»]
ProteinModelPortaliQ6YN16.
SMRiQ6YN16. Positions 1-276, 326-418.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6YN16.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini306 – 415110SCP2Add
BLAST

Sequence similaritiesi

Contains 1 SCP2 domain.Curated

Phylogenomic databases

eggNOGiCOG1028.
GeneTreeiENSGT00730000111071.
HOVERGENiHBG107982.
InParanoidiQ6YN16.
OMAiWFKNHTA.
OrthoDBiEOG77T155.
PhylomeDBiQ6YN16.
TreeFamiTF101523.

Family and domain databases

Gene3Di3.30.1050.10. 1 hit.
3.40.50.720. 2 hits.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR003033. SCP2_sterol-bd_dom.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
PF02036. SCP2. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF55718. SSF55718. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6YN16-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLPNTGRLAG CTVFITGASR GIGKAIALKA AKDGANIVIA AKTAQPHPKL
60 70 80 90 100
LGTIYTAAEE IEAVGGKALP CIVDVRDEQQ ISAAVEKAIK KFGGIDILVN
110 120 130 140 150
NASAISLTNT LDTPTKRLDL MMNVNTRGTY LASKACIPYL KKSKVAHILN
160 170 180 190 200
ISPPLNLNPV WFKQHCAYTI AKYGMSMYVL GMAEEFKGEI AVNALWPKTA
210 220 230 240 250
IHTAAMDMLG GPGIESQCRK VDIIADAAYS IFQKPKSFTG NFVIDENILK
260 270 280 290 300
EEGIENFDVY AIKPGHPLQP DFFLDEYPEA VSKKVESTGA VPEFKEEKLQ
310 320 330 340 350
LQPKPRSGAV EETFRIVKDS LSDDVVKATQ AIYLFELSGE DGGTWFLDLK
360 370 380 390 400
SKGGNVGYGE PSDQADVVMS MTTDDFVKMF SGKLKPTMAF MSGKLKIKGN
410
MALAIKLEKL MNQMNARL
Length:418
Mass (Da):45,395
Last modified:July 5, 2004 - v1
Checksum:iA8B27CC7D6F1B636
GO
Isoform 2 (identifier: Q6YN16-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     94-166: Missing.

Show »
Length:345
Mass (Da):37,320
Checksum:iA8CE6A7AC163A2D1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti62 – 621E → G in BAF82618. (PubMed:14702039)Curated
Sequence conflicti89 – 891I → V in BAF85175. (PubMed:14702039)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei94 – 16673Missing in isoform 2. 2 PublicationsVSP_031529Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY093428 mRNA. Translation: AAM14670.1.
AK292486 mRNA. Translation: BAF85175.1.
AK289929 mRNA. Translation: BAF82618.1.
AL162732 Genomic DNA. Translation: CAM17151.1.
AL162732 Genomic DNA. Translation: CAM17152.1.
CH471105 Genomic DNA. Translation: EAW59105.1.
CH471105 Genomic DNA. Translation: EAW59106.1.
BC004331 mRNA. Translation: AAH04331.1.
BC095451 mRNA. Translation: AAH95451.1.
AL833735 mRNA. Translation: CAH56256.1.
CCDSiCCDS43864.1. [Q6YN16-1]
CCDS56582.1. [Q6YN16-2]
RefSeqiNP_001182751.1. NM_001195822.1. [Q6YN16-2]
NP_115679.2. NM_032303.4. [Q6YN16-1]
UniGeneiHs.59486.

Genome annotation databases

EnsembliENST00000398803; ENSP00000381783; ENSG00000119471. [Q6YN16-2]
ENST00000398805; ENSP00000381785; ENSG00000119471. [Q6YN16-1]
GeneIDi84263.
KEGGihsa:84263.
UCSCiuc004bga.2. human. [Q6YN16-1]
uc004bgc.2. human. [Q6YN16-2]

Polymorphism databases

DMDMi74749521.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY093428 mRNA. Translation: AAM14670.1.
AK292486 mRNA. Translation: BAF85175.1.
AK289929 mRNA. Translation: BAF82618.1.
AL162732 Genomic DNA. Translation: CAM17151.1.
AL162732 Genomic DNA. Translation: CAM17152.1.
CH471105 Genomic DNA. Translation: EAW59105.1.
CH471105 Genomic DNA. Translation: EAW59106.1.
BC004331 mRNA. Translation: AAH04331.1.
BC095451 mRNA. Translation: AAH95451.1.
AL833735 mRNA. Translation: CAH56256.1.
CCDSiCCDS43864.1. [Q6YN16-1]
CCDS56582.1. [Q6YN16-2]
RefSeqiNP_001182751.1. NM_001195822.1. [Q6YN16-2]
NP_115679.2. NM_032303.4. [Q6YN16-1]
UniGeneiHs.59486.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3KVOX-ray2.25A/B1-293[»]
ProteinModelPortaliQ6YN16.
SMRiQ6YN16. Positions 1-276, 326-418.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123990. 6 interactions.
IntActiQ6YN16. 2 interactions.
STRINGi9606.ENSP00000381785.

PTM databases

PhosphoSiteiQ6YN16.

Polymorphism databases

DMDMi74749521.

2D gel databases

UCD-2DPAGEQ6YN16.

Proteomic databases

MaxQBiQ6YN16.
PaxDbiQ6YN16.
PRIDEiQ6YN16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000398803; ENSP00000381783; ENSG00000119471. [Q6YN16-2]
ENST00000398805; ENSP00000381785; ENSG00000119471. [Q6YN16-1]
GeneIDi84263.
KEGGihsa:84263.
UCSCiuc004bga.2. human. [Q6YN16-1]
uc004bgc.2. human. [Q6YN16-2]

Organism-specific databases

CTDi84263.
GeneCardsiGC09P115142.
HGNCiHGNC:18572. HSDL2.
HPAiHPA042890.
HPA050453.
neXtProtiNX_Q6YN16.
PharmGKBiPA134980105.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1028.
GeneTreeiENSGT00730000111071.
HOVERGENiHBG107982.
InParanoidiQ6YN16.
OMAiWFKNHTA.
OrthoDBiEOG77T155.
PhylomeDBiQ6YN16.
TreeFamiTF101523.

Miscellaneous databases

ChiTaRSiHSDL2. human.
EvolutionaryTraceiQ6YN16.
GenomeRNAii84263.
NextBioi73798.
PROiQ6YN16.

Gene expression databases

BgeeiQ6YN16.
CleanExiHS_HSDL2.
ExpressionAtlasiQ6YN16. baseline and differential.
GenevestigatoriQ6YN16.

Family and domain databases

Gene3Di3.30.1050.10. 1 hit.
3.40.50.720. 2 hits.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR003033. SCP2_sterol-bd_dom.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
PF02036. SCP2. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF55718. SSF55718. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of a novel human hydroxysteroid dehydrogenase-like 2 (HSDL2) cDNA from fetal brain."
    Dai J., Xie Y., Wu Q., Wang L., Yin G., Ye X., Zeng L., Xu J., Ji C., Gu S., Huang Q., Zhao R.C., Mao Y.
    Biochem. Genet. 41:165-174(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Fetal brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Hippocampus and Testis.
  3. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Blood vessel and Uterus.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 78-418 (ISOFORM 1).
    Tissue: Stomach.
  7. "In search for function of two human orphan SDR enzymes: Hydroxysteroid dehydrogenase like 2 (HSDL2) and short-chain dehydrogenase/reductase-orphan (SDR-O)."
    Kowalik D., Haller F., Adamski J., Moeller G.
    J. Steroid Biochem. Mol. Biol. 117:117-124(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Crystal structure of the catalytic domain of human hydroxysteroid dehydrogenase like 2 (HSDL2)."
    Structural genomics consortium (SGC)
    Submitted (MAR-2010) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 1-293 IN COMPLEX WITH NADP.

Entry informationi

Entry nameiHSDL2_HUMAN
AccessioniPrimary (citable) accession number: Q6YN16
Secondary accession number(s): A8K1L4
, A8K8X1, A8MSV3, Q658M8, Q9BT58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: July 5, 2004
Last modified: January 7, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.