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Q6YN16 (HSDL2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxysteroid dehydrogenase-like protein 2

EC=1.-.-.-
Gene names
Name:HSDL2
Synonyms:C9orf99
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length418 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has apparently no steroid dehydrogenase activity. Ref.7

Subcellular location

Peroxisome Ref.7.

Tissue specificity

Ubiquitous. Ref.1

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Contains 1 SCP2 domain.

Ontologies

Keywords
   Cellular componentPeroxisome
   Coding sequence diversityAlternative splicing
   LigandNADP
   Molecular functionOxidoreductase
   PTMAcetylation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentmitochondrion

Inferred from electronic annotation. Source: Ensembl

peroxisome

Inferred from direct assay Ref.7. Source: UniProtKB

   Molecular_functionoxidoreductase activity

Inferred from electronic annotation. Source: UniProtKB-KW

sterol binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6YN16-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6YN16-2)

The sequence of this isoform differs from the canonical sequence as follows:
     94-166: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 418418Hydroxysteroid dehydrogenase-like protein 2
PRO_0000319888

Regions

Domain306 – 415110SCP2
Nucleotide binding17 – 237NADP

Sites

Active site1681Proton acceptor Potential
Binding site421NADP
Binding site741NADP
Binding site1721NADP

Amino acid modifications

Modified residue1161N6-acetyllysine By similarity
Modified residue3181N6-succinyllysine By similarity

Natural variations

Alternative sequence94 – 16673Missing in isoform 2.
VSP_031529

Experimental info

Sequence conflict621E → G in BAF82618. Ref.2
Sequence conflict891I → V in BAF85175. Ref.2

Secondary structure

................................................. 418
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: A8B27CC7D6F1B636

FASTA41845,395
        10         20         30         40         50         60 
MLPNTGRLAG CTVFITGASR GIGKAIALKA AKDGANIVIA AKTAQPHPKL LGTIYTAAEE 

        70         80         90        100        110        120 
IEAVGGKALP CIVDVRDEQQ ISAAVEKAIK KFGGIDILVN NASAISLTNT LDTPTKRLDL 

       130        140        150        160        170        180 
MMNVNTRGTY LASKACIPYL KKSKVAHILN ISPPLNLNPV WFKQHCAYTI AKYGMSMYVL 

       190        200        210        220        230        240 
GMAEEFKGEI AVNALWPKTA IHTAAMDMLG GPGIESQCRK VDIIADAAYS IFQKPKSFTG 

       250        260        270        280        290        300 
NFVIDENILK EEGIENFDVY AIKPGHPLQP DFFLDEYPEA VSKKVESTGA VPEFKEEKLQ 

       310        320        330        340        350        360 
LQPKPRSGAV EETFRIVKDS LSDDVVKATQ AIYLFELSGE DGGTWFLDLK SKGGNVGYGE 

       370        380        390        400        410 
PSDQADVVMS MTTDDFVKMF SGKLKPTMAF MSGKLKIKGN MALAIKLEKL MNQMNARL 

« Hide

Isoform 2 [UniParc].

Checksum: A8CE6A7AC163A2D1
Show »

FASTA34537,320

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of a novel human hydroxysteroid dehydrogenase-like 2 (HSDL2) cDNA from fetal brain."
Dai J., Xie Y., Wu Q., Wang L., Yin G., Ye X., Zeng L., Xu J., Ji C., Gu S., Huang Q., Zhao R.C., Mao Y.
Biochem. Genet. 41:165-174(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Tissue: Fetal brain.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Hippocampus and Testis.
[3]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Blood vessel and Uterus.
[6]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 78-418 (ISOFORM 1).
Tissue: Stomach.
[7]"In search for function of two human orphan SDR enzymes: Hydroxysteroid dehydrogenase like 2 (HSDL2) and short-chain dehydrogenase/reductase-orphan (SDR-O)."
Kowalik D., Haller F., Adamski J., Moeller G.
J. Steroid Biochem. Mol. Biol. 117:117-124(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Crystal structure of the catalytic domain of human hydroxysteroid dehydrogenase like 2 (HSDL2)."
Structural genomics consortium (SGC)
Submitted (MAR-2010) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 1-293 IN COMPLEX WITH NADP.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY093428 mRNA. Translation: AAM14670.1.
AK292486 mRNA. Translation: BAF85175.1.
AK289929 mRNA. Translation: BAF82618.1.
AL162732 Genomic DNA. Translation: CAM17151.1.
AL162732 Genomic DNA. Translation: CAM17152.1.
CH471105 Genomic DNA. Translation: EAW59105.1.
CH471105 Genomic DNA. Translation: EAW59106.1.
BC004331 mRNA. Translation: AAH04331.1.
BC095451 mRNA. Translation: AAH95451.1.
AL833735 mRNA. Translation: CAH56256.1.
RefSeqNP_001182751.1. NM_001195822.1.
NP_115679.2. NM_032303.4.
UniGeneHs.59486.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3KVOX-ray2.25A/B1-293[»]
ProteinModelPortalQ6YN16.
SMRQ6YN16. Positions 1-276, 326-418.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123990. 7 interactions.
IntActQ6YN16. 2 interactions.
STRING9606.ENSP00000381785.

PTM databases

PhosphoSiteQ6YN16.

Polymorphism databases

DMDM74749521.

2D gel databases

UCD-2DPAGEQ6YN16.

Proteomic databases

PaxDbQ6YN16.
PRIDEQ6YN16.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000398803; ENSP00000381783; ENSG00000119471. [Q6YN16-2]
ENST00000398805; ENSP00000381785; ENSG00000119471. [Q6YN16-1]
GeneID84263.
KEGGhsa:84263.
UCSCuc004bga.2. human. [Q6YN16-1]
uc004bgc.2. human. [Q6YN16-2]

Organism-specific databases

CTD84263.
GeneCardsGC09P115142.
HGNCHGNC:18572. HSDL2.
HPAHPA042890.
HPA050453.
neXtProtNX_Q6YN16.
PharmGKBPA134980105.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1028.
HOVERGENHBG107982.
InParanoidQ6YN16.
OMAWFKNHTA.
OrthoDBEOG77T155.
PhylomeDBQ6YN16.
TreeFamTF101523.

Gene expression databases

ArrayExpressQ6YN16.
BgeeQ6YN16.
CleanExHS_HSDL2.
GenevestigatorQ6YN16.

Family and domain databases

Gene3D3.30.1050.10. 1 hit.
3.40.50.720. 2 hits.
InterProIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR003033. SCP2_sterol-bd_dom.
[Graphical view]
PfamPF00106. adh_short. 1 hit.
PF02036. SCP2. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
SUPFAMSSF55718. SSF55718. 1 hit.
PROSITEPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ6YN16.
GenomeRNAi84263.
NextBio73798.
PROQ6YN16.

Entry information

Entry nameHSDL2_HUMAN
AccessionPrimary (citable) accession number: Q6YN16
Secondary accession number(s): A8K1L4 expand/collapse secondary AC list , A8K8X1, A8MSV3, Q658M8, Q9BT58
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM