Reviewed, UniProtKB/Swiss-Prot Q6YM50 (MAN5_SOLLC)

Names and origin

Protein names

Recommended name:
    Mannan endo-1,4-beta-mannosidase 5
    EC=3.2.1.78
Alternative name(s):
    Beta-mannanase 5
    Endo-beta-1,4-mannanase 5
    LeMAN5

Gene names

Name:

MAN5

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum)

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May be involved in weakening of anther wall during pollen development.
Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Subcellular location	Secreted Potential.
Developmental stage	Expressed in anthers and pollen of unopened bud and opening flowers, but not in fully opened flowers. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 414	389	Mannan endo-1,4-beta-mannosidase 5		PRO_5000088873
Sites
	Active site	211	1	Proton donor By similarity
	Active site	328	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6YM50-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 2ED692448896046E	FASTA	414	46,835
	10 20 30 40 50 60 MAYFQRLISC IFVLFLLSLA FACEARVLLD ENNANDQGFV RVNGAHFELN GSPFLFNGFN 70 80 90 100 110 120 SYWLMHVAAE PSERYKVSEV LREASSAGLS VCRTWAFSDG GDRALQISPG VYDERVFQGL 130 140 150 160 170 180 DFVISEAKKY GIRLILSFVN NYNDFGGKAQ YVQWARNAGA QINGDDDFYT NYITKNYYKN 190 200 210 220 230 240 HIKKVVTRFN TITGMTYKDD STIMAWELMN EPRNQADYSG NTLNAWVQEM ASFVKSLDNK 250 260 270 280 290 300 HLLEIGMEGF YGDSVPERKS INPGYQVGTD FISNHLIKEI DFATIHAYTD QWLSGQSDDA 310 320 330 340 350 360 QMIFMQKWMT SHWQDAKNIL KKPLVLAEFG KSSRDPGYNQ NIRDTFMSTI YRNIYSLAKD 370 380 390 400 410 GGTMGGSLIW QLVAQGMENY EDGYCIELGK NPSTAGIITS QSHAMTALAH LVKI

References

[1]

"A novel endo-beta-mannanase gene in tomato LeMAN5 is associated with anther and pollen development." Filichkin S.A., Leonard J.M., Monteros A., Liu P.-P., Nonogaki H. Plant Physiol. 134:1080-1087(2004) [PubMed: 14976239] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE. Strain: cv. Moneymaker.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY102168 Genomic DNA. Translation: AAM26920.1.
UniGene	Les.108
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Enzyme and pathway databases
BRENDA	3.2.1.78. 281054.
Family and domain databases
InterPro	IPR001547. Glyco_hydro_5. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAN5_SOLLC

Accession

Primary (citable) accession number: Q6YM50

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	July 5, 2004
Last modified:	November 24, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)