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Protein

Mannan endo-1,4-beta-mannosidase 5

Gene

MAN5

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

May be involved in weakening of anther wall during pollen development.

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei211 – 2111Proton donorBy similarity
Active sitei328 – 3281NucleophileBy similarity

GO - Molecular functioni

  1. mannan endo-1,4-beta-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,4-beta-mannosidase 5 (EC:3.2.1.78)
Alternative name(s):
Beta-mannanase 5
Endo-beta-1,4-mannanase 5
LeMAN5
Gene namesi
Name:MAN5
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
ProteomesiUP000004994: Chromosome 6

Subcellular locationi

Secreted Curated

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 414389Mannan endo-1,4-beta-mannosidase 5PRO_5000088873Add
BLAST

Expressioni

Developmental stagei

Expressed in anthers and pollen of unopened bud and opening flowers, but not in fully opened flowers.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ6YM50.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiQ6YM50.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6YM50-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAYFQRLISC IFVLFLLSLA FACEARVLLD ENNANDQGFV RVNGAHFELN
60 70 80 90 100
GSPFLFNGFN SYWLMHVAAE PSERYKVSEV LREASSAGLS VCRTWAFSDG
110 120 130 140 150
GDRALQISPG VYDERVFQGL DFVISEAKKY GIRLILSFVN NYNDFGGKAQ
160 170 180 190 200
YVQWARNAGA QINGDDDFYT NYITKNYYKN HIKKVVTRFN TITGMTYKDD
210 220 230 240 250
STIMAWELMN EPRNQADYSG NTLNAWVQEM ASFVKSLDNK HLLEIGMEGF
260 270 280 290 300
YGDSVPERKS INPGYQVGTD FISNHLIKEI DFATIHAYTD QWLSGQSDDA
310 320 330 340 350
QMIFMQKWMT SHWQDAKNIL KKPLVLAEFG KSSRDPGYNQ NIRDTFMSTI
360 370 380 390 400
YRNIYSLAKD GGTMGGSLIW QLVAQGMENY EDGYCIELGK NPSTAGIITS
410
QSHAMTALAH LVKI
Length:414
Mass (Da):46,835
Last modified:July 5, 2004 - v1
Checksum:i2ED692448896046E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY102168 Genomic DNA. Translation: AAM26920.1.
RefSeqiNP_001234473.1. NM_001247544.1.
UniGeneiLes.108.

Genome annotation databases

EnsemblPlantsiSolyc06g064520.2.1; Solyc06g064520.2.1; Solyc06g064520.2.
GeneIDi544170.
KEGGisly:544170.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY102168 Genomic DNA. Translation: AAM26920.1.
RefSeqiNP_001234473.1. NM_001247544.1.
UniGeneiLes.108.

3D structure databases

ProteinModelPortaliQ6YM50.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiSolyc06g064520.2.1; Solyc06g064520.2.1; Solyc06g064520.2.
GeneIDi544170.
KEGGisly:544170.

Phylogenomic databases

InParanoidiQ6YM50.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "A novel endo-beta-mannanase gene in tomato LeMAN5 is associated with anther and pollen development."
    Filichkin S.A., Leonard J.M., Monteros A., Liu P.-P., Nonogaki H.
    Plant Physiol. 134:1080-1087(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE.
    Strain: cv. Moneymaker.

Entry informationi

Entry nameiMAN5_SOLLC
AccessioniPrimary (citable) accession number: Q6YM50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: July 5, 2004
Last modified: January 7, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.