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Protein

Non-structural protein 1

Gene
N/A
Organism
Rotavirus A (strain RVA/Monkey/United States/RRV/1980/G3P5B[3]) (RV-A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri44 – 81Sequence analysisAdd BLAST38

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host IRF7 by virus, Inhibition of host NF-kappa-B by virus, Inhibition of host RLR pathway by virus, Interferon antiviral system evasion, Viral immunoevasion

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 1
Short name:
NSP1
Alternative name(s):
NCVP2
Non-structural RNA-binding protein 53
Short name:
NS53
OrganismiRotavirus A (strain RVA/Monkey/United States/RRV/1980/G3P5B[3]) (RV-A)
Taxonomic identifieri10969 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiMacaca mulatta (Rhesus macaque) [TaxID: 9544]

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003690901 – 494Non-structural protein 1Add BLAST494

Interactioni

Subunit structurei

Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation (By similarity).By similarity

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 83RNA-bindingBy similarityAdd BLAST83
Regioni84 – 178Important for cytoskeleton localizationBy similarityAdd BLAST95
Regioni316 – 494Interaction with IRF3By similarityAdd BLAST179

Domaini

The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity (By similarity).By similarity

Sequence similaritiesi

Belongs to the rotavirus A NSP1 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri44 – 81Sequence analysisAdd BLAST38

Keywords - Domaini

Zinc-finger

Family and domain databases

InterProiIPR002148. Rotavirus_NSP1.
[Graphical view]
PfamiPF00981. Rota_NS53. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6YLT2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATFKDACFH YRRVTKLNRE LLRIGANSVW TPVSSNKIKI KGWCIECCQL
60 70 80 90 100
TGLTFCHGCS LAHVCQWCIQ NKRCFLDNEP HLLKLRTFES PITKEKLQCI
110 120 130 140 150
INLYELLFPI NHGVINKFKK TIKQRKCRNE FDKSWYNQLL LPITLNAAVF
160 170 180 190 200
KFHSRDVYVF GFYEGSSPCI DLPYRLVNCI DLYDKLLLDQ VNFERMSSLP
210 220 230 240 250
DNLQSIYANK YFKLSRLPSM KLKRIYYSDF SKQNLINKYK TKSRIVLRNL
260 270 280 290 300
TEFTWDSQTD LHHDLINDKD KILAALSTSS LKQFETHDLN LGRIKADIFE
310 320 330 340 350
LGHHCKPNYI SSNHWQPASK ISKCKWCNVK YAFRDMDWKM ESMYNELLSF
360 370 380 390 400
IQSCYKSNVN VGHCSSIEKA YPLVKDILWH SITEYIDQTV EKLFNTMNPV
410 420 430 440 450
QVNEQQVIKF CWQIDIALYM HIKMILETEA LPFTFTLNQF NSIIKGIVNQ
460 470 480 490
WCDVAELDHL PLCTEQTDAL VKLEEEGKLS EEYELLISDS EDDD
Length:494
Mass (Da):58,010
Last modified:April 14, 2009 - v2
Checksum:i56C1EC0D832CEF10
GO

Sequence cautioni

The sequence AAA50496 differs from that shown. Reason: Frameshift at position 407.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50L → V in AAM92466 (PubMed:15372078).Curated1
Sequence conflicti74C → Y in CAA83546 (Ref. 3) Curated1
Sequence conflicti85L → F in CAA83546 (Ref. 3) Curated1
Sequence conflicti280S → F in CAA83546 (Ref. 3) Curated1
Sequence conflicti434T → I in CAA83546 (Ref. 3) Curated1
Sequence conflicti482E → G in AAA50496 (PubMed:8030275).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08433 Genomic RNA. Translation: AAA50496.1. Frameshift.
AY117048 mRNA. Translation: AAM92466.1.
Z32535 Genomic RNA. Translation: CAA83546.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08433 Genomic RNA. Translation: AAA50496.1. Frameshift.
AY117048 mRNA. Translation: AAM92466.1.
Z32535 Genomic RNA. Translation: CAA83546.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR002148. Rotavirus_NSP1.
[Graphical view]
PfamiPF00981. Rota_NS53. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNSP1_ROTRH
AccessioniPrimary (citable) accession number: Q6YLT2
Secondary accession number(s): Q86280, Q88349
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 14, 2009
Last modified: November 30, 2016
This is version 39 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.