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Q6YLT2 (NSP1_ROTRH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Non-structural protein 1

Short name=NSP1
Alternative name(s):
NCVP2
Non-structural RNA-binding protein 53
Short name=NS53
OrganismRotavirus A (strain Monkey/United States/RRV/1980 G3-P5B[3]-I2-R2-C3-M3-A9-Nx-T3-E3-H6) (RV-A)
Taxonomic identifier10969 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostMacaca mulatta (Rhesus macaque) [TaxID: 9544]

Protein attributes

Sequence length494 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation By similarity.

Subunit structure

Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation By similarity.

Subcellular location

Host cytoplasmhost cytoskeleton By similarity.

Domain

The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity By similarity.

Sequence similarities

Belongs to the rotavirus A NSP1 family.

Sequence caution

The sequence AAA50496.1 differs from that shown. Reason: Frameshift at position 407.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 494494Non-structural protein 1
PRO_0000369090

Regions

Zinc finger44 – 8138 Potential
Region1 – 8383RNA-binding By similarity
Region84 – 17895Important for cytoskeleton localization By similarity
Region316 – 494179Interaction with IRF3 By similarity

Experimental info

Sequence conflict501L → V in AAM92466. Ref.2
Sequence conflict741C → Y in CAA83546. Ref.3
Sequence conflict851L → F in CAA83546. Ref.3
Sequence conflict2801S → F in CAA83546. Ref.3
Sequence conflict4341T → I in CAA83546. Ref.3
Sequence conflict4821E → G in AAA50496. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q6YLT2 [UniParc].

Last modified April 14, 2009. Version 2.
Checksum: 56C1EC0D832CEF10

FASTA49458,010
        10         20         30         40         50         60 
MATFKDACFH YRRVTKLNRE LLRIGANSVW TPVSSNKIKI KGWCIECCQL TGLTFCHGCS 

        70         80         90        100        110        120 
LAHVCQWCIQ NKRCFLDNEP HLLKLRTFES PITKEKLQCI INLYELLFPI NHGVINKFKK 

       130        140        150        160        170        180 
TIKQRKCRNE FDKSWYNQLL LPITLNAAVF KFHSRDVYVF GFYEGSSPCI DLPYRLVNCI 

       190        200        210        220        230        240 
DLYDKLLLDQ VNFERMSSLP DNLQSIYANK YFKLSRLPSM KLKRIYYSDF SKQNLINKYK 

       250        260        270        280        290        300 
TKSRIVLRNL TEFTWDSQTD LHHDLINDKD KILAALSTSS LKQFETHDLN LGRIKADIFE 

       310        320        330        340        350        360 
LGHHCKPNYI SSNHWQPASK ISKCKWCNVK YAFRDMDWKM ESMYNELLSF IQSCYKSNVN 

       370        380        390        400        410        420 
VGHCSSIEKA YPLVKDILWH SITEYIDQTV EKLFNTMNPV QVNEQQVIKF CWQIDIALYM 

       430        440        450        460        470        480 
HIKMILETEA LPFTFTLNQF NSIIKGIVNQ WCDVAELDHL PLCTEQTDAL VKLEEEGKLS 

       490 
EEYELLISDS EDDD 

« Hide

References

[1]"Comparison of the rotavirus nonstructural protein NSP1 (NS53) from different species by sequence analysis and northern blot hybridization."
Dunn S.J., Cross T.L., Greenberg H.B.
Virology 203:178-183(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Cell-line-induced mutation of the rotavirus genome alters expression of an IRF3-interacting protein."
Kearney K., Chen D., Taraporewala Z.F., Vende P., Hoshino Y., Tortorici M.A., Barro M., Patton J.T.
EMBO J. 23:4072-4081(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]McCrae M.A.
Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U08433 Genomic RNA. Translation: AAA50496.1. Frameshift.
AY117048 mRNA. Translation: AAM92466.1.
Z32535 Genomic RNA. Translation: CAA83546.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002148. Rotavirus_NSP1.
[Graphical view]
PfamPF00981. Rota_NS53. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNSP1_ROTRH
AccessionPrimary (citable) accession number: Q6YLT2
Secondary accession number(s): Q86280, Q88349
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 14, 2009
Last modified: February 19, 2014
This is version 35 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families