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Q6YLT2

- NSP1_ROTRH

UniProt

Q6YLT2 - NSP1_ROTRH

Protein

Non-structural protein 1

Gene
N/A
Organism
Rotavirus A (strain Monkey/United States/RRV/1980 G3-P5B[3]-I2-R2-C3-M3-A9-Nx-T3-E3-H6) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 36 (01 Oct 2014)
      Sequence version 2 (14 Apr 2009)
      Previous versions | rss
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    Functioni

    Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri44 – 8138Sequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. suppression by virus of host IRF3 activity Source: UniProtKB-KW
    2. suppression by virus of host IRF7 activity Source: UniProtKB-KW
    3. suppression by virus of host NF-kappaB transcription factor activity Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host IRF7 by virus, Inhibition of host NF-kappa-B by virus, Inhibition of host RLR pathway by virus, Interferon antiviral system evasion, Viral immunoevasion

    Keywords - Ligandi

    Metal-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Non-structural protein 1
    Short name:
    NSP1
    Alternative name(s):
    NCVP2
    Non-structural RNA-binding protein 53
    Short name:
    NS53
    OrganismiRotavirus A (strain Monkey/United States/RRV/1980 G3-P5B[3]-I2-R2-C3-M3-A9-Nx-T3-E3-H6) (RV-A)
    Taxonomic identifieri10969 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
    Virus hostiMacaca mulatta (Rhesus macaque) [TaxID: 9544]

    Subcellular locationi

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-KW
    2. host cytoskeleton Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Host cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 494494Non-structural protein 1PRO_0000369090Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation By similarity.By similarity

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 8383RNA-bindingBy similarityAdd
    BLAST
    Regioni84 – 17895Important for cytoskeleton localizationBy similarityAdd
    BLAST
    Regioni316 – 494179Interaction with IRF3By similarityAdd
    BLAST

    Domaini

    The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity By similarity.By similarity

    Sequence similaritiesi

    Belongs to the rotavirus A NSP1 family.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri44 – 8138Sequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Family and domain databases

    InterProiIPR002148. Rotavirus_NSP1.
    [Graphical view]
    PfamiPF00981. Rota_NS53. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6YLT2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATFKDACFH YRRVTKLNRE LLRIGANSVW TPVSSNKIKI KGWCIECCQL    50
    TGLTFCHGCS LAHVCQWCIQ NKRCFLDNEP HLLKLRTFES PITKEKLQCI 100
    INLYELLFPI NHGVINKFKK TIKQRKCRNE FDKSWYNQLL LPITLNAAVF 150
    KFHSRDVYVF GFYEGSSPCI DLPYRLVNCI DLYDKLLLDQ VNFERMSSLP 200
    DNLQSIYANK YFKLSRLPSM KLKRIYYSDF SKQNLINKYK TKSRIVLRNL 250
    TEFTWDSQTD LHHDLINDKD KILAALSTSS LKQFETHDLN LGRIKADIFE 300
    LGHHCKPNYI SSNHWQPASK ISKCKWCNVK YAFRDMDWKM ESMYNELLSF 350
    IQSCYKSNVN VGHCSSIEKA YPLVKDILWH SITEYIDQTV EKLFNTMNPV 400
    QVNEQQVIKF CWQIDIALYM HIKMILETEA LPFTFTLNQF NSIIKGIVNQ 450
    WCDVAELDHL PLCTEQTDAL VKLEEEGKLS EEYELLISDS EDDD 494
    Length:494
    Mass (Da):58,010
    Last modified:April 14, 2009 - v2
    Checksum:i56C1EC0D832CEF10
    GO

    Sequence cautioni

    The sequence AAA50496.1 differs from that shown. Reason: Frameshift at position 407.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti50 – 501L → V in AAM92466. (PubMed:15372078)Curated
    Sequence conflicti74 – 741C → Y in CAA83546. 1 PublicationCurated
    Sequence conflicti85 – 851L → F in CAA83546. 1 PublicationCurated
    Sequence conflicti280 – 2801S → F in CAA83546. 1 PublicationCurated
    Sequence conflicti434 – 4341T → I in CAA83546. 1 PublicationCurated
    Sequence conflicti482 – 4821E → G in AAA50496. (PubMed:8030275)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U08433 Genomic RNA. Translation: AAA50496.1. Frameshift.
    AY117048 mRNA. Translation: AAM92466.1.
    Z32535 Genomic RNA. Translation: CAA83546.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U08433 Genomic RNA. Translation: AAA50496.1 . Frameshift.
    AY117048 mRNA. Translation: AAM92466.1 .
    Z32535 Genomic RNA. Translation: CAA83546.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR002148. Rotavirus_NSP1.
    [Graphical view ]
    Pfami PF00981. Rota_NS53. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparison of the rotavirus nonstructural protein NSP1 (NS53) from different species by sequence analysis and northern blot hybridization."
      Dunn S.J., Cross T.L., Greenberg H.B.
      Virology 203:178-183(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Cell-line-induced mutation of the rotavirus genome alters expression of an IRF3-interacting protein."
      Kearney K., Chen D., Taraporewala Z.F., Vende P., Hoshino Y., Tortorici M.A., Barro M., Patton J.T.
      EMBO J. 23:4072-4081(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. McCrae M.A.
      Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiNSP1_ROTRH
    AccessioniPrimary (citable) accession number: Q6YLT2
    Secondary accession number(s): Q86280, Q88349
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: April 14, 2009
    Last modified: October 1, 2014
    This is version 36 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3