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Q6YLT2

- NSP1_ROTRH

UniProt

Q6YLT2 - NSP1_ROTRH

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Protein
Non-structural protein 1
Gene
N/A
Organism
Rotavirus A (strain Monkey/United States/RRV/1980 G3-P5B[3]-I2-R2-C3-M3-A9-Nx-T3-E3-H6) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri44 – 8138 Reviewed prediction
Add
BLAST

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. suppression by virus of host IRF3 activity Source: UniProtKB-KW
  2. suppression by virus of host IRF7 activity Source: UniProtKB-KW
  3. suppression by virus of host NF-kappaB transcription factor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host IRF7 by virus, Inhibition of host NF-kappa-B by virus, Inhibition of host RLR pathway by virus, Interferon antiviral system evasion, Viral immunoevasion

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 1
Short name:
NSP1
Alternative name(s):
NCVP2
Non-structural RNA-binding protein 53
Short name:
NS53
OrganismiRotavirus A (strain Monkey/United States/RRV/1980 G3-P5B[3]-I2-R2-C3-M3-A9-Nx-T3-E3-H6) (RV-A)
Taxonomic identifieri10969 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiMacaca mulatta (Rhesus macaque) [TaxID: 9544]

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cytoskeleton Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Non-structural protein 1
PRO_0000369090Add
BLAST

Interactioni

Subunit structurei

Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation By similarity.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 8383RNA-binding By similarity
Add
BLAST
Regioni84 – 17895Important for cytoskeleton localization By similarity
Add
BLAST
Regioni316 – 494179Interaction with IRF3 By similarity
Add
BLAST

Domaini

The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity By similarity.

Sequence similaritiesi

Belongs to the rotavirus A NSP1 family.

Keywords - Domaini

Zinc-finger

Family and domain databases

InterProiIPR002148. Rotavirus_NSP1.
[Graphical view]
PfamiPF00981. Rota_NS53. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6YLT2-1 [UniParc]FASTAAdd to Basket

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MATFKDACFH YRRVTKLNRE LLRIGANSVW TPVSSNKIKI KGWCIECCQL    50
TGLTFCHGCS LAHVCQWCIQ NKRCFLDNEP HLLKLRTFES PITKEKLQCI 100
INLYELLFPI NHGVINKFKK TIKQRKCRNE FDKSWYNQLL LPITLNAAVF 150
KFHSRDVYVF GFYEGSSPCI DLPYRLVNCI DLYDKLLLDQ VNFERMSSLP 200
DNLQSIYANK YFKLSRLPSM KLKRIYYSDF SKQNLINKYK TKSRIVLRNL 250
TEFTWDSQTD LHHDLINDKD KILAALSTSS LKQFETHDLN LGRIKADIFE 300
LGHHCKPNYI SSNHWQPASK ISKCKWCNVK YAFRDMDWKM ESMYNELLSF 350
IQSCYKSNVN VGHCSSIEKA YPLVKDILWH SITEYIDQTV EKLFNTMNPV 400
QVNEQQVIKF CWQIDIALYM HIKMILETEA LPFTFTLNQF NSIIKGIVNQ 450
WCDVAELDHL PLCTEQTDAL VKLEEEGKLS EEYELLISDS EDDD 494
Length:494
Mass (Da):58,010
Last modified:April 14, 2009 - v2
Checksum:i56C1EC0D832CEF10
GO

Sequence cautioni

The sequence AAA50496.1 differs from that shown. Reason: Frameshift at position 407.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501L → V in AAM92466. 1 Publication
Sequence conflicti74 – 741C → Y in CAA83546. 1 Publication
Sequence conflicti85 – 851L → F in CAA83546. 1 Publication
Sequence conflicti280 – 2801S → F in CAA83546. 1 Publication
Sequence conflicti434 – 4341T → I in CAA83546. 1 Publication
Sequence conflicti482 – 4821E → G in AAA50496. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U08433 Genomic RNA. Translation: AAA50496.1. Frameshift.
AY117048 mRNA. Translation: AAM92466.1.
Z32535 Genomic RNA. Translation: CAA83546.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U08433 Genomic RNA. Translation: AAA50496.1 . Frameshift.
AY117048 mRNA. Translation: AAM92466.1 .
Z32535 Genomic RNA. Translation: CAA83546.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002148. Rotavirus_NSP1.
[Graphical view ]
Pfami PF00981. Rota_NS53. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparison of the rotavirus nonstructural protein NSP1 (NS53) from different species by sequence analysis and northern blot hybridization."
    Dunn S.J., Cross T.L., Greenberg H.B.
    Virology 203:178-183(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Cell-line-induced mutation of the rotavirus genome alters expression of an IRF3-interacting protein."
    Kearney K., Chen D., Taraporewala Z.F., Vende P., Hoshino Y., Tortorici M.A., Barro M., Patton J.T.
    EMBO J. 23:4072-4081(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. McCrae M.A.
    Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNSP1_ROTRH
AccessioniPrimary (citable) accession number: Q6YLT2
Secondary accession number(s): Q86280, Q88349
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 14, 2009
Last modified: February 19, 2014
This is version 35 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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