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Q6YLT2

- NSP1_ROTRH

UniProt

Q6YLT2 - NSP1_ROTRH

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Protein

Non-structural protein 1

Gene
N/A
Organism
Rotavirus A (strain Monkey/United States/RRV/1980 G3-P5B[3]-I2-R2-C3-M3-A9-Nx-T3-E3-H6) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri44 – 8138Sequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. suppression by virus of host IRF3 activity Source: UniProtKB-KW
  2. suppression by virus of host IRF7 activity Source: UniProtKB-KW
  3. suppression by virus of host NF-kappaB transcription factor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host IRF7 by virus, Inhibition of host NF-kappa-B by virus, Inhibition of host RLR pathway by virus, Interferon antiviral system evasion, Viral immunoevasion

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 1
Short name:
NSP1
Alternative name(s):
NCVP2
Non-structural RNA-binding protein 53
Short name:
NS53
OrganismiRotavirus A (strain Monkey/United States/RRV/1980 G3-P5B[3]-I2-R2-C3-M3-A9-Nx-T3-E3-H6) (RV-A)
Taxonomic identifieri10969 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiMacaca mulatta (Rhesus macaque) [TaxID: 9544]

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cytoskeleton Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Non-structural protein 1PRO_0000369090Add
BLAST

Interactioni

Subunit structurei

Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation (By similarity).By similarity

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 8383RNA-bindingBy similarityAdd
BLAST
Regioni84 – 17895Important for cytoskeleton localizationBy similarityAdd
BLAST
Regioni316 – 494179Interaction with IRF3By similarityAdd
BLAST

Domaini

The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity (By similarity).By similarity

Sequence similaritiesi

Belongs to the rotavirus A NSP1 family.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri44 – 8138Sequence AnalysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Family and domain databases

InterProiIPR002148. Rotavirus_NSP1.
[Graphical view]
PfamiPF00981. Rota_NS53. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6YLT2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATFKDACFH YRRVTKLNRE LLRIGANSVW TPVSSNKIKI KGWCIECCQL
60 70 80 90 100
TGLTFCHGCS LAHVCQWCIQ NKRCFLDNEP HLLKLRTFES PITKEKLQCI
110 120 130 140 150
INLYELLFPI NHGVINKFKK TIKQRKCRNE FDKSWYNQLL LPITLNAAVF
160 170 180 190 200
KFHSRDVYVF GFYEGSSPCI DLPYRLVNCI DLYDKLLLDQ VNFERMSSLP
210 220 230 240 250
DNLQSIYANK YFKLSRLPSM KLKRIYYSDF SKQNLINKYK TKSRIVLRNL
260 270 280 290 300
TEFTWDSQTD LHHDLINDKD KILAALSTSS LKQFETHDLN LGRIKADIFE
310 320 330 340 350
LGHHCKPNYI SSNHWQPASK ISKCKWCNVK YAFRDMDWKM ESMYNELLSF
360 370 380 390 400
IQSCYKSNVN VGHCSSIEKA YPLVKDILWH SITEYIDQTV EKLFNTMNPV
410 420 430 440 450
QVNEQQVIKF CWQIDIALYM HIKMILETEA LPFTFTLNQF NSIIKGIVNQ
460 470 480 490
WCDVAELDHL PLCTEQTDAL VKLEEEGKLS EEYELLISDS EDDD
Length:494
Mass (Da):58,010
Last modified:April 14, 2009 - v2
Checksum:i56C1EC0D832CEF10
GO

Sequence cautioni

The sequence AAA50496.1 differs from that shown. Reason: Frameshift at position 407. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501L → V in AAM92466. (PubMed:15372078)Curated
Sequence conflicti74 – 741C → Y in CAA83546. 1 PublicationCurated
Sequence conflicti85 – 851L → F in CAA83546. 1 PublicationCurated
Sequence conflicti280 – 2801S → F in CAA83546. 1 PublicationCurated
Sequence conflicti434 – 4341T → I in CAA83546. 1 PublicationCurated
Sequence conflicti482 – 4821E → G in AAA50496. (PubMed:8030275)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08433 Genomic RNA. Translation: AAA50496.1. Frameshift.
AY117048 mRNA. Translation: AAM92466.1.
Z32535 Genomic RNA. Translation: CAA83546.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08433 Genomic RNA. Translation: AAA50496.1 . Frameshift.
AY117048 mRNA. Translation: AAM92466.1 .
Z32535 Genomic RNA. Translation: CAA83546.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002148. Rotavirus_NSP1.
[Graphical view ]
Pfami PF00981. Rota_NS53. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparison of the rotavirus nonstructural protein NSP1 (NS53) from different species by sequence analysis and northern blot hybridization."
    Dunn S.J., Cross T.L., Greenberg H.B.
    Virology 203:178-183(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Cell-line-induced mutation of the rotavirus genome alters expression of an IRF3-interacting protein."
    Kearney K., Chen D., Taraporewala Z.F., Vende P., Hoshino Y., Tortorici M.A., Barro M., Patton J.T.
    EMBO J. 23:4072-4081(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. McCrae M.A.
    Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNSP1_ROTRH
AccessioniPrimary (citable) accession number: Q6YLT2
Secondary accession number(s): Q86280, Q88349
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 14, 2009
Last modified: October 29, 2014
This is version 37 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3