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Q6YI93 (SETB2_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Histone-lysine N-methyltransferase SETDB2

EC=2.1.1.43
Alternative name(s):
Chronic lymphocytic leukemia deletion region gene 8 protein homolog
SET domain bifurcated 2
Gene names
Name:setdb2
Synonyms:clld8
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length703 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis By similarity.

Catalytic activity

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].

Subcellular location

Nucleus By similarity. Chromosome By similarity.

Domain

In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster By similarity.

Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily.

Contains 1 MBD (methyl-CpG-binding) domain.

Contains 1 pre-SET domain.

Contains 1 SET domain.

Sequence caution

The sequence AAN61106.1 differs from that shown. Reason: Frameshift at position 695.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 703703Histone-lysine N-methyltransferase SETDB2
PRO_0000281825

Regions

Domain178 – 24871MBD
Domain310 – 38475Pre-SET
Domain387 – 678292SET
Region397 – 3993S-adenosyl-L-methionine binding By similarity
Region635 – 6362S-adenosyl-L-methionine binding By similarity

Sites

Metal binding3121Zinc 1 By similarity
Metal binding3121Zinc 2 By similarity
Metal binding3141Zinc 1 By similarity
Metal binding3181Zinc 1 By similarity
Metal binding3181Zinc 3 By similarity
Metal binding3241Zinc 1 By similarity
Metal binding3261Zinc 2 By similarity
Metal binding3651Zinc 2 By similarity
Metal binding3651Zinc 3 By similarity
Metal binding3691Zinc 2 By similarity
Metal binding3711Zinc 3 By similarity
Metal binding3761Zinc 3 By similarity
Metal binding6381Zinc 4 By similarity
Metal binding6911Zinc 4 By similarity
Metal binding6931Zinc 4 By similarity
Metal binding6981Zinc 4 By similarity
Binding site6321S-adenosyl-L-methionine By similarity

Experimental info

Sequence conflict971K → Q in AAN61106. Ref.1
Sequence conflict1751S → P in AAN61106. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q6YI93 [UniParc].

Last modified October 5, 2010. Version 2.
Checksum: 12D86C32892B5325

FASTA70378,698
        10         20         30         40         50         60 
MEQSANARQS TLRSRTQELN TLSVLSKDVS LEDAKKYWKD RQADGKVDWI FEKVLNKLKI 

        70         80         90        100        110        120 
LWQKIKDGSA TNLEYVRAVI LVNEAGNLEE DLEEDLKEDT DTIHIDIHKE NEVQENTDCS 

       130        140        150        160        170        180 
PERKEDTCLN LNTDCGTDVS GSEPECNSTV SPPAAERVYF GNHSCGPSCL SGINSFLFTK 

       190        200        210        220        230        240 
GNPLQLPISC DFQRCHLKIN SPDDLSHILY KAPCGRSLRD YDEVHSYLTE TGCHFLAVDN 

       250        260        270        280        290        300 
FSFNNHVRLD SNSSFNQGIV QDCDISNDVE SVPVAFSNEI DNTRPSNFIY RKTSWPPGYS 

       310        320        330        340        350        360 
LNNFTDIFVK CCNCTDGCLD ILTCSCLQLT AQAFTKCMES SLGIGPLGYK HKRLQEPIPT 

       370        380        390        400        410        420 
GLYECNVSCK CDRMLCQNRV VQHGLKLRLQ VFKTNTKGWG VRCLDDVDKG TFVCIYAGRI 

       430        440        450        460        470        480 
LIRTADCTVK STPDDSVACG NEDHEDSTST CALILSKRKR KTSHSDSEVT VMHTNPYSMR 

       490        500        510        520        530        540 
SHGLSVHRLS NTFSPRQARS GEREFSLQPL RRPKTKTSML QKRRRQLIEE GACTVQNSSE 

       550        560        570        580        590        600 
EEGPTPPQSP EQKSSAGTKI QRNENSDETA SGYVSEESSS SVISGGHPLE KPISKFKSKL 

       610        620        630        640        650        660 
NKTTVYLSTS PEQTCEENLH FLDASKEGNV GRFLNHSCCP NLFVQQVFVD THQKCFPWVA 

       670        680        690        700 
FFTNSVVKAG TELTWDYSYD IGTAADQEIQ CLCGQKTCKN KVV 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and analysis of the expression of SET-domain putative histone methyltransferase CLLD8 in Xenopus laevis."
Ruzov A., Meehan R.
Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]NIH - Xenopus Gene Collection (XGC) project
Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Oocyte.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY145835 mRNA. Translation: AAN61106.1. Frameshift.
BC170303 mRNA. Translation: AAI70303.1.
RefSeqNP_001082765.1. NM_001089296.1.
UniGeneXl.29790.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID398711.
KEGGxla:398711.

Organism-specific databases

CTD83852.
XenbaseXB-GENE-1219036. setdb2.

Phylogenomic databases

HOVERGENHBG106688.
KOK11421.

Family and domain databases

InterProIPR016177. DNA-bd_dom.
IPR001739. Methyl_CpG_DNA-bd.
IPR003616. Post-SET_dom.
IPR007728. Pre-SET_dom.
IPR001214. SET_dom.
[Graphical view]
PfamPF01429. MBD. 1 hit.
PF05033. Pre-SET. 1 hit.
PF00856. SET. 1 hit.
[Graphical view]
SMARTSM00317. SET. 1 hit.
[Graphical view]
SUPFAMSSF54171. SSF54171. 1 hit.
PROSITEPS50868. POST_SET. 1 hit.
PS50867. PRE_SET. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSETB2_XENLA
AccessionPrimary (citable) accession number: Q6YI93
Secondary accession number(s): B7ZRV6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: October 5, 2010
Last modified: May 14, 2014
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families