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Q6YDN9

- XTH_BRAOB

UniProt

Q6YDN9 - XTH_BRAOB

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Protein

Xyloglucan endotransglucosylase/hydrolase

Gene

XET16A

Organism
Brassica oleracea var. botrytis (Cauliflower)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei108 – 1081NucleophilePROSITE-ProRule annotation
Active sitei112 – 1121Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
  2. xyloglucan:xyloglucosyl transferase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular glucan metabolic process Source: InterPro
  2. cell wall organization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BRENDAi2.4.1.207. 948.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Xyloglucan endotransglucosylase/hydrolase (EC:2.4.1.207)
Alternative name(s):
BobXET16A
Gene namesi
Name:XET16A
OrganismiBrassica oleracea var. botrytis (Cauliflower)
Taxonomic identifieri3715 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: UniProtKB-KW
  2. cell wall Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 295272Xyloglucan endotransglucosylase/hydrolasePRO_0000011834Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi116 – 1161N-linked (GlcNAc...)1 Publication

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity
The N-glycan consists of an (GlcNAc)2(Hex)6 oligosaccharide; not essential for its enzymatic activity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ6YDN9.

Structurei

3D structure databases

ProteinModelPortaliQ6YDN9.
SMRiQ6YDN9. Positions 30-295.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6YDN9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVSSTPWAL VALFLMASST VMAIPPRKAI DVPFGRNYVP TWAFDHQKQL
60 70 80 90 100
NGGSELQLIL DKYTGTGFQS KGSYLFGHFS MHIKLPAGDT AGVVTAFYLS
110 120 130 140 150
STNNEHDEID FEFLGNRTGQ PVILQTNVFT GGKGNREQRI YLWFDPSKAY
160 170 180 190 200
HTYSVLWNLY QIVFFVDNIP IRVFKNAKDL GVRFPFNQPM KLYSSLWNAD
210 220 230 240 250
DWATRGGLEK TNWANAPFIA SYRGFHIDGC QASVEAKYCA TQGRMWWDQN
260 270 280 290
EFRDLDAEQY RRLKWVRMKW TIYNYCTDRT RFPVMPAECR RDRDV
Length:295
Mass (Da):34,105
Last modified:July 5, 2004 - v1
Checksum:i15054A3E4929BD4E
GO

Mass spectrometryi

Molecular mass is 33115 Da from positions 24 - 295. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY156708 mRNA. Translation: AAO00727.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY156708 mRNA. Translation: AAO00727.1 .

3D structure databases

ProteinModelPortali Q6YDN9.
SMRi Q6YDN9. Positions 30-295.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH16. Glycoside Hydrolase Family 16.

Proteomic databases

PRIDEi Q6YDN9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BRENDAi 2.4.1.207. 948.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view ]
Pfami PF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF005604. XET. 1 hit.
SUPFAMi SSF49899. SSF49899. 1 hit.
PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "N-linked glycosylation of native and recombinant cauliflower xyloglucan endotransglycosylase 16A."
    Henriksson H., Denman S.E., Campuzano I.D.G., Ademark P., Master E.R., Teeri T.T., Brumer H. III
    Biochem. J. 375:61-73(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-38 AND 75-133, MASS SPECTROMETRY, GLYCOSYLATION.

Entry informationi

Entry nameiXTH_BRAOB
AccessioniPrimary (citable) accession number: Q6YDN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

No hydrolytic activity detected in vitro.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3