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Q6YDN9

- XTH_BRAOB

UniProt

Q6YDN9 - XTH_BRAOB

Protein

Xyloglucan endotransglucosylase/hydrolase

Gene

XET16A

Organism
Brassica oleracea var. botrytis (Cauliflower)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.

    Catalytic activityi

    Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei108 – 1081NucleophilePROSITE-ProRule annotation
    Active sitei112 – 1121Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
    2. xyloglucan:xyloglucosyl transferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular glucan metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Transferase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BRENDAi2.4.1.207. 948.

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xyloglucan endotransglucosylase/hydrolase (EC:2.4.1.207)
    Alternative name(s):
    BobXET16A
    Gene namesi
    Name:XET16A
    OrganismiBrassica oleracea var. botrytis (Cauliflower)
    Taxonomic identifieri3715 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell
    2. cell wall Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Apoplast, Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 23231 PublicationAdd
    BLAST
    Chaini24 – 295272Xyloglucan endotransglucosylase/hydrolasePRO_0000011834Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi116 – 1161N-linked (GlcNAc...)1 Publication

    Post-translational modificationi

    Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity
    The N-glycan consists of an (GlcNAc)2(Hex)6 oligosaccharide; not essential for its enzymatic activity.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ6YDN9.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6YDN9.
    SMRiQ6YDN9. Positions 30-295.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005604. XET. 1 hit.
    SUPFAMiSSF49899. SSF49899. 1 hit.
    PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q6YDN9-1 [UniParc]FASTAAdd to Basket

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    MAVSSTPWAL VALFLMASST VMAIPPRKAI DVPFGRNYVP TWAFDHQKQL    50
    NGGSELQLIL DKYTGTGFQS KGSYLFGHFS MHIKLPAGDT AGVVTAFYLS 100
    STNNEHDEID FEFLGNRTGQ PVILQTNVFT GGKGNREQRI YLWFDPSKAY 150
    HTYSVLWNLY QIVFFVDNIP IRVFKNAKDL GVRFPFNQPM KLYSSLWNAD 200
    DWATRGGLEK TNWANAPFIA SYRGFHIDGC QASVEAKYCA TQGRMWWDQN 250
    EFRDLDAEQY RRLKWVRMKW TIYNYCTDRT RFPVMPAECR RDRDV 295
    Length:295
    Mass (Da):34,105
    Last modified:July 5, 2004 - v1
    Checksum:i15054A3E4929BD4E
    GO

    Mass spectrometryi

    Molecular mass is 33115 Da from positions 24 - 295. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY156708 mRNA. Translation: AAO00727.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY156708 mRNA. Translation: AAO00727.1 .

    3D structure databases

    ProteinModelPortali Q6YDN9.
    SMRi Q6YDN9. Positions 30-295.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Proteomic databases

    PRIDEi Q6YDN9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BRENDAi 2.4.1.207. 948.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005604. XET. 1 hit.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "N-linked glycosylation of native and recombinant cauliflower xyloglucan endotransglycosylase 16A."
      Henriksson H., Denman S.E., Campuzano I.D.G., Ademark P., Master E.R., Teeri T.T., Brumer H. III
      Biochem. J. 375:61-73(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-38 AND 75-133, MASS SPECTROMETRY, GLYCOSYLATION.

    Entry informationi

    Entry nameiXTH_BRAOB
    AccessioniPrimary (citable) accession number: Q6YDN9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 23, 2004
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    No hydrolytic activity detected in vitro.

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3