Q6YDN9 (XTH_BRAOB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Xyloglucan endotransglucosylase/hydrolase
EC=2.4.1.207

Alternative name(s):
BobXET16A

Gene names

Name:

XET16A

Organism

Brassica oleracea var. botrytis (Cauliflower)

Taxonomic identifier

3715 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Brassica › Brassica oleracea

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.
Catalytic activity	Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.
Subcellular location	Secreted › cell wall Probable. Secreted › extracellular space › apoplast Probable.
Post-translational modification	Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity. The N-glycan consists of an (GlcNAc)2(Hex)6 oligosaccharide; not essential for its enzymatic activity.
Miscellaneous	No hydrolytic activity detected in vitro.
Sequence similarities	Belongs to the glycosyl hydrolase 16 family. XTH group 1 subfamily.
Mass spectrometry	Molecular mass is 33115 Da from positions 24 - 295. Determined by ESI. Ref.1

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Apoplast Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cellular glucan metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro xyloglucan:xyloglucosyl transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.1
	Chain	24 – 295	272	Xyloglucan endotransglucosylase/hydrolase		PRO_0000011834
Sites
	Active site	108	1	Nucleophile By similarity
	Active site	112	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	116	1	N-linked (GlcNAc...)

Sequences

	Sequence		Length	Mass (Da)
	Q6YDN9 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 15054A3E4929BD4E	FASTA	295	34,105
	10 20 30 40 50 60 MAVSSTPWAL VALFLMASST VMAIPPRKAI DVPFGRNYVP TWAFDHQKQL NGGSELQLIL 70 80 90 100 110 120 DKYTGTGFQS KGSYLFGHFS MHIKLPAGDT AGVVTAFYLS STNNEHDEID FEFLGNRTGQ 130 140 150 160 170 180 PVILQTNVFT GGKGNREQRI YLWFDPSKAY HTYSVLWNLY QIVFFVDNIP IRVFKNAKDL 190 200 210 220 230 240 GVRFPFNQPM KLYSSLWNAD DWATRGGLEK TNWANAPFIA SYRGFHIDGC QASVEAKYCA 250 260 270 280 290 TQGRMWWDQN EFRDLDAEQY RRLKWVRMKW TIYNYCTDRT RFPVMPAECR RDRDV

References

[1]

"N-linked glycosylation of native and recombinant cauliflower xyloglucan endotransglycosylase 16A." Henriksson H., Denman S.E., Campuzano I.D.G., Ademark P., Master E.R., Teeri T.T., Brumer H. III Biochem. J. 375:61-73(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-38 AND 75-133, MASS SPECTROMETRY, GLYCOSYLATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY156708 mRNA. Translation: AAO00727.1.
3D structure databases
ProteinModelPortal	Q6YDN9.
SMR	Q6YDN9. Positions 30-295.
ModBase	Search...
Protein family/group databases
CAZy	GH16. Glycoside Hydrolase Family 16.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	2.4.1.207. 948.
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. IPR008263. Glycoside_hydrolase_16_AS. IPR016455. XET. IPR010713. XET_C. [Graphical view]
Pfam	PF00722. Glyco_hydro_16. 1 hit. PF06955. XET_C. 1 hit. [Graphical view]
PIRSF	PIRSF005604. XET. 1 hit.
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS01034. GLYCOSYL_HYDROL_F16. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XTH_BRAOB

Accession

Primary (citable) accession number: Q6YDN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program