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Q6YDN9 (XTH_BRAOB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Xyloglucan endotransglucosylase/hydrolase

EC=2.4.1.207
Alternative name(s):
BobXET16A
Gene names
Name:XET16A
OrganismBrassica oleracea var. botrytis (Cauliflower)
Taxonomic identifier3715 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Protein attributes

Sequence length295 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.

Catalytic activity

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Subcellular location

Secretedcell wall Probable. Secretedextracellular spaceapoplast Probable.

Post-translational modification

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.

The N-glycan consists of an (GlcNAc)2(Hex)6 oligosaccharide; not essential for its enzymatic activity.

Miscellaneous

No hydrolytic activity detected in vitro.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family. XTH group 1 subfamily.

Mass spectrometry

Molecular mass is 33115 Da from positions 24 - 295. Determined by ESI. Ref.1

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Ref.1
Chain24 – 295272Xyloglucan endotransglucosylase/hydrolase
PRO_0000011834

Sites

Active site1081Nucleophile By similarity
Active site1121Proton donor By similarity

Amino acid modifications

Glycosylation1161N-linked (GlcNAc...)

Sequences

Sequence LengthMass (Da)Tools
Q6YDN9 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 15054A3E4929BD4E

FASTA29534,105
        10         20         30         40         50         60 
MAVSSTPWAL VALFLMASST VMAIPPRKAI DVPFGRNYVP TWAFDHQKQL NGGSELQLIL 

        70         80         90        100        110        120 
DKYTGTGFQS KGSYLFGHFS MHIKLPAGDT AGVVTAFYLS STNNEHDEID FEFLGNRTGQ 

       130        140        150        160        170        180 
PVILQTNVFT GGKGNREQRI YLWFDPSKAY HTYSVLWNLY QIVFFVDNIP IRVFKNAKDL 

       190        200        210        220        230        240 
GVRFPFNQPM KLYSSLWNAD DWATRGGLEK TNWANAPFIA SYRGFHIDGC QASVEAKYCA 

       250        260        270        280        290 
TQGRMWWDQN EFRDLDAEQY RRLKWVRMKW TIYNYCTDRT RFPVMPAECR RDRDV 

« Hide

References

[1]"N-linked glycosylation of native and recombinant cauliflower xyloglucan endotransglycosylase 16A."
Henriksson H., Denman S.E., Campuzano I.D.G., Ademark P., Master E.R., Teeri T.T., Brumer H. III
Biochem. J. 375:61-73(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-38 AND 75-133, MASS SPECTROMETRY, GLYCOSYLATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY156708 mRNA. Translation: AAO00727.1.

3D structure databases

ProteinModelPortalQ6YDN9.
SMRQ6YDN9. Positions 30-295.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Proteomic databases

PRIDEQ6YDN9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA2.4.1.207. 948.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFPIRSF005604. XET. 1 hit.
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameXTH_BRAOB
AccessionPrimary (citable) accession number: Q6YDN9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: July 5, 2004
Last modified: February 19, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries