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Protein

Proton-coupled amino acid transporter 4

Gene

SLC36A4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a sodium-independent electroneutral transporter for tryptophan, proline and alanine. Inhibited by sarcosine.1 Publication

Kineticsi

  1. KM=3.1 µM for L-proline1 Publication
  2. KM=1.7 µM for L-tryptophan1 Publication

    pH dependencei

    Optimum pH is 7.4 (for proline transport).1 Publication

    GO - Molecular functioni

    • L-alanine transmembrane transporter activity Source: WormBase
    • L-proline transmembrane transporter activity Source: WormBase
    • L-tryptophan transmembrane transporter activity Source: WormBase
    • symporter activity Source: UniProtKB-KW

    GO - Biological processi

    • L-alanine transport Source: UniProtKB
    • proline transmembrane transport Source: GOC
    • proline transport Source: UniProtKB
    • tryptophan transport Source: UniProtKB
    Complete GO annotation...

    Keywords - Biological processi

    Amino-acid transport, Symport, Transport

    Protein family/group databases

    TCDBi2.A.18.8.5. the amino acid/auxin permease (aaap) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proton-coupled amino acid transporter 4
    Short name:
    Proton/amino acid transporter 4
    Alternative name(s):
    Solute carrier family 36 member 4
    Gene namesi
    Name:SLC36A4
    Synonyms:PAT4
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:19660. SLC36A4.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei87 – 10721HelicalSequence analysisAdd
    BLAST
    Transmembranei154 – 17421HelicalSequence analysisAdd
    BLAST
    Transmembranei206 – 22621HelicalSequence analysisAdd
    BLAST
    Transmembranei231 – 25121HelicalSequence analysisAdd
    BLAST
    Transmembranei273 – 29321HelicalSequence analysisAdd
    BLAST
    Transmembranei309 – 32921HelicalSequence analysisAdd
    BLAST
    Transmembranei358 – 37821HelicalSequence analysisAdd
    BLAST
    Transmembranei389 – 40921HelicalSequence analysisAdd
    BLAST
    Transmembranei414 – 43421HelicalSequence analysisAdd
    BLAST
    Transmembranei456 – 47621HelicalSequence analysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134908925.

    Polymorphism and mutation databases

    BioMutaiSLC36A4.
    DMDMi74749514.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 504504Proton-coupled amino acid transporter 4PRO_0000308318Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineCombined sources
    Glycosylationi495 – 4951N-linked (GlcNAc...)Sequence analysis

    Keywords - PTMi

    Acetylation, Glycoprotein

    Proteomic databases

    EPDiQ6YBV0.
    MaxQBiQ6YBV0.
    PaxDbiQ6YBV0.
    PRIDEiQ6YBV0.

    PTM databases

    iPTMnetiQ6YBV0.
    PhosphoSiteiQ6YBV0.

    Expressioni

    Gene expression databases

    BgeeiQ6YBV0.
    CleanExiHS_SLC36A4.
    ExpressionAtlasiQ6YBV0. baseline and differential.
    GenevisibleiQ6YBV0. HS.

    Organism-specific databases

    HPAiHPA017887.

    Interactioni

    Protein-protein interaction databases

    IntActiQ6YBV0. 1 interaction.
    STRINGi9606.ENSP00000317382.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6YBV0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1304. Eukaryota.
    COG0814. LUCA.
    GeneTreeiENSGT00390000011245.
    HOGENOMiHOG000006823.
    HOVERGENiHBG057183.
    InParanoidiQ6YBV0.
    KOiK14209.
    OMAiILTFSKE.
    OrthoDBiEOG73V6KC.
    PhylomeDBiQ6YBV0.
    TreeFamiTF314873.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q6YBV0-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MEAAATPAAA GAARREELDM DVMRPLINEQ NFDGTSDEEH EQELLPVQKH
    60 70 80 90 100
    YQLDDQEGIS FVQTLMHLLK GNIGTGLLGL PLAIKNAGIV LGPISLVFIG
    110 120 130 140 150
    IISVHCMHIL VRCSHFLCLR FKKSTLGYSD TVSFAMEVSP WSCLQKQAAW
    160 170 180 190 200
    GRSVVDFFLV ITQLGFCSVY IVFLAENVKQ VHEGFLESKV FISNSTNSSN
    210 220 230 240 250
    PCERRSVDLR IYMLCFLPFI ILLVFIRELK NLFVLSFLAN VSMAVSLVII
    260 270 280 290 300
    YQYVVRNMPD PHNLPIVAGW KKYPLFFGTA VFAFEGIGVV LPLENQMKES
    310 320 330 340 350
    KRFPQALNIG MGIVTTLYVT LATLGYMCFH DEIKGSITLN LPQDVWLYQS
    360 370 380 390 400
    VKILYSFGIF VTYSIQFYVP AEIIIPGITS KFHTKWKQIC EFGIRSFLVS
    410 420 430 440 450
    ITCAGAILIP RLDIVISFVG AVSSSTLALI LPPLVEILTF SKEHYNIWMV
    460 470 480 490 500
    LKNISIAFTG VVGFLLGTYI TVEEIIYPTP KVVAGTPQSP FLNLNSTCLT

    SGLK
    Length:504
    Mass (Da):56,157
    Last modified:July 5, 2004 - v1
    Checksum:i066CCC585EB2F170
    GO
    Isoform 2 (identifier: Q6YBV0-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-135: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:369
    Mass (Da):41,385
    Checksum:iF2F3C1A051D9752C
    GO

    Sequence cautioni

    The sequence CAC82496.1 differs from that shown. Reason: Frameshift at positions 358, 361, 363, 366, 370, 373, 376, 387, 396, 458 and 487. Curated

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti330 – 3301H → R in BAC04737 (PubMed:14702039).Curated
    Sequence conflicti387 – 3871K → E in CAC82496 (Ref. 4) Curated
    Sequence conflicti391 – 3922EF → GI in CAC82496 (Ref. 4) Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti209 – 2091L → I.1 Publication
    Corresponds to variant rs17854446 [ dbSNP | Ensembl ].
    VAR_036791
    Natural varianti376 – 3761P → H.1 Publication
    Corresponds to variant rs17854445 [ dbSNP | Ensembl ].
    VAR_036792
    Natural varianti429 – 4291L → I.1 Publication
    Corresponds to variant rs17854443 [ dbSNP | Ensembl ].
    VAR_036793

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 135135Missing in isoform 2. 1 PublicationVSP_028959Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY162216 mRNA. Translation: AAO11790.1.
    AK096251 mRNA. Translation: BAC04737.1.
    BC047374 mRNA. Translation: AAH47374.1.
    AJ295983 mRNA. Translation: CAC82496.1. Frameshift.
    CCDSiCCDS66202.1. [Q6YBV0-2]
    CCDS8291.1. [Q6YBV0-1]
    RefSeqiNP_001273068.1. NM_001286139.1. [Q6YBV0-2]
    NP_689526.2. NM_152313.3. [Q6YBV0-1]
    UniGeneiHs.148766.
    Hs.596383.

    Genome annotation databases

    EnsembliENST00000326402; ENSP00000317382; ENSG00000180773. [Q6YBV0-1]
    ENST00000529184; ENSP00000436570; ENSG00000180773. [Q6YBV0-2]
    GeneIDi120103.
    KEGGihsa:120103.
    UCSCiuc001pdl.3. human. [Q6YBV0-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY162216 mRNA. Translation: AAO11790.1.
    AK096251 mRNA. Translation: BAC04737.1.
    BC047374 mRNA. Translation: AAH47374.1.
    AJ295983 mRNA. Translation: CAC82496.1. Frameshift.
    CCDSiCCDS66202.1. [Q6YBV0-2]
    CCDS8291.1. [Q6YBV0-1]
    RefSeqiNP_001273068.1. NM_001286139.1. [Q6YBV0-2]
    NP_689526.2. NM_152313.3. [Q6YBV0-1]
    UniGeneiHs.148766.
    Hs.596383.

    3D structure databases

    ProteinModelPortaliQ6YBV0.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    IntActiQ6YBV0. 1 interaction.
    STRINGi9606.ENSP00000317382.

    Protein family/group databases

    TCDBi2.A.18.8.5. the amino acid/auxin permease (aaap) family.

    PTM databases

    iPTMnetiQ6YBV0.
    PhosphoSiteiQ6YBV0.

    Polymorphism and mutation databases

    BioMutaiSLC36A4.
    DMDMi74749514.

    Proteomic databases

    EPDiQ6YBV0.
    MaxQBiQ6YBV0.
    PaxDbiQ6YBV0.
    PRIDEiQ6YBV0.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000326402; ENSP00000317382; ENSG00000180773. [Q6YBV0-1]
    ENST00000529184; ENSP00000436570; ENSG00000180773. [Q6YBV0-2]
    GeneIDi120103.
    KEGGihsa:120103.
    UCSCiuc001pdl.3. human. [Q6YBV0-1]

    Organism-specific databases

    CTDi120103.
    GeneCardsiSLC36A4.
    HGNCiHGNC:19660. SLC36A4.
    HPAiHPA017887.
    MIMi613760. gene.
    neXtProtiNX_Q6YBV0.
    PharmGKBiPA134908925.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG1304. Eukaryota.
    COG0814. LUCA.
    GeneTreeiENSGT00390000011245.
    HOGENOMiHOG000006823.
    HOVERGENiHBG057183.
    InParanoidiQ6YBV0.
    KOiK14209.
    OMAiILTFSKE.
    OrthoDBiEOG73V6KC.
    PhylomeDBiQ6YBV0.
    TreeFamiTF314873.

    Miscellaneous databases

    ChiTaRSiSLC36A4. human.
    GenomeRNAii120103.
    PROiQ6YBV0.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ6YBV0.
    CleanExiHS_SLC36A4.
    ExpressionAtlasiQ6YBV0. baseline and differential.
    GenevisibleiQ6YBV0. HS.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "A cluster of proton/amino acid transporter (PAT) genes in the human and murine genome."
      Boll M., Foltz M., Rubio-Aliaga I., Kolmeder D., Daniel H.
      Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Brain.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS ILE-209 AND HIS-376 AND ILE-429.
      Tissue: Brain.
    4. "Full length sequencing of some human and murine muscular transcripts (Telethon Italy project B41)."
      Ievolella C., Zara I., Millino C., Faulkner G., Lanfranchi G.
      Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 162-504.
      Tissue: Skeletal muscle.
    5. "SLC36A4 (hPAT4) is a high affinity amino acid transporter when expressed in Xenopus laevis oocytes."
      Pillai S.M., Meredith D.
      J. Biol. Chem. 286:2455-2460(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    6. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiS36A4_HUMAN
    AccessioniPrimary (citable) accession number: Q6YBV0
    Secondary accession number(s): Q86X30, Q8IVM5, Q8N8S6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 23, 2007
    Last sequence update: July 5, 2004
    Last modified: June 8, 2016
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.