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Protein

GRB10-interacting GYF protein 2

Gene

Gigyf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Key component of the 4EHP-GYF2 complex, a multiprotein complex that acts as a repressor of translation initiation (PubMed:26763119). In 4EHP-GYF2 the complex, acts as a factor that bridges EIF4E2 to ZFP36/TTP, linking translation repression with mRNA decay (PubMed:26763119). May act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling, including IGF1 and insulin receptors (PubMed:12771153).2 Publications

GO - Molecular functioni

GO - Biological processi

  • adult locomotory behavior Source: MGI
  • cellular protein metabolic process Source: MGI
  • feeding behavior Source: MGI
  • homeostasis of number of cells within a tissue Source: MGI
  • insulin-like growth factor receptor signaling pathway Source: MGI
  • mitotic G1 DNA damage checkpoint Source: MGI
  • mRNA destabilization Source: MGI
  • multicellular organism growth Source: MGI
  • musculoskeletal movement Source: MGI
  • negative regulation of translation Source: MGI
  • neuromuscular process controlling balance Source: MGI
  • post-embryonic development Source: MGI
  • posttranscriptional gene silencing Source: MGI
  • spinal cord motor neuron differentiation Source: MGI

Names & Taxonomyi

Protein namesi
Recommended name:
GRB10-interacting GYF protein 21 Publication
Alternative name(s):
PERQ amino acid-rich with GYF domain-containing protein 2
Trinucleotide repeat-containing gene 15 protein
Gene namesi
Name:Gigyf21 Publication
Synonyms:Kiaa0642, Perq2, Tnrc15
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2138584. Gigyf2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: ParkinsonsUK-UCL
  • cytoplasmic stress granule Source: MGI
  • cytosol Source: MGI
  • endoplasmic reticulum Source: MGI
  • endosome Source: MGI
  • Golgi apparatus Source: MGI
  • integral component of membrane Source: ParkinsonsUK-UCL
  • membrane Source: MGI
  • perikaryon Source: ParkinsonsUK-UCL
  • proximal dendrite Source: ParkinsonsUK-UCL
  • vesicle Source: ParkinsonsUK-UCL

Pathology & Biotechi

Disruption phenotypei

Mice undergo normal embryonic development, but fail to feed and die within the first 2 postnatal days. Heterozygous mice survive to adulthood with no evident metabolic or growth defects. At 12-15 months of age, heterozygous mice show motor dysfunction associated with histopathologic evidence of neurodegeneration and rare intracytoplasmic Lewy body-like inclusions in spinal anterior horn motor neurons.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002708382 – 1291GRB10-interacting GYF protein 2Add BLAST1290

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei19PhosphoserineBy similarity1
Modified residuei26PhosphoserineCombined sources1
Modified residuei30PhosphoserineCombined sources1
Modified residuei107Omega-N-methylarginineCombined sources1
Modified residuei119Omega-N-methylarginineCombined sources1
Modified residuei121Omega-N-methylarginineCombined sources1
Modified residuei140PhosphoserineBy similarity1
Modified residuei150Omega-N-methylarginineCombined sources1
Modified residuei161PhosphoserineBy similarity1
Modified residuei190PhosphoserineBy similarity1
Modified residuei237PhosphoserineBy similarity1
Modified residuei383PhosphothreonineCombined sources1
Modified residuei594PhosphoserineBy similarity1
Modified residuei995PhosphoserineBy similarity1
Modified residuei1276PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ6Y7W8.
PaxDbiQ6Y7W8.
PeptideAtlasiQ6Y7W8.
PRIDEiQ6Y7W8.

PTM databases

iPTMnetiQ6Y7W8.
PhosphoSitePlusiQ6Y7W8.

Expressioni

Tissue specificityi

Expressed in heart, liver, kidney and brain as well as in testis (PubMed:12771153).1 Publication

Developmental stagei

Widely expressed in embryonic tissues, including strong expression in the central nervous system.1 Publication

Gene expression databases

BgeeiENSMUSG00000048000.
CleanExiMM_GIGYF2.
ExpressionAtlasiQ6Y7W8. baseline and differential.
GenevisibleiQ6Y7W8. MM.

Interactioni

Subunit structurei

Component of the 4EHP-GYF2 complex, at least composed of EIF4E2, GIGYF2 and ZNF598 (PubMed:26763119). Interacts (via the 4EHP-binding motif) with EIF4E2; the interaction is direct (By similarity). Interacts with ZFP36/TTP (via P-P-P-P-G repeats); the interaction is direct (PubMed:26763119). Interacts with GRB10 (PubMed:12771153).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi230615. 1 interactor.
IntActiQ6Y7W8. 1 interactor.
MINTiMINT-266607.
STRINGi10090.ENSMUSP00000027475.

Structurei

3D structure databases

ProteinModelPortaliQ6Y7W8.
SMRiQ6Y7W8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini534 – 582GYFPROSITE-ProRule annotationAdd BLAST49

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni548 – 564Required for GRB10-bindingAdd BLAST17

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi119 – 273Arg-richAdd BLAST155
Compositional biasi437 – 474Pro-richAdd BLAST38
Compositional biasi608 – 1031Gln-richAdd BLAST424
Compositional biasi739 – 889Glu-richAdd BLAST151
Compositional biasi1204 – 1244Gln-richAdd BLAST41

Sequence similaritiesi

Belongs to the GIGYF family.Curated

Phylogenomic databases

eggNOGiKOG1862. Eukaryota.
ENOG4111Q0Y. LUCA.
GeneTreeiENSGT00660000095550.
HOVERGENiHBG082121.
InParanoidiQ6Y7W8.
KOiK18730.
OMAiSMKFGPE.
OrthoDBiEOG091G0FKG.
PhylomeDBiQ6Y7W8.
TreeFamiTF325513.

Family and domain databases

CDDicd00072. GYF. 1 hit.
Gene3Di2.130.10.10. 1 hit.
InterProiView protein in InterPro
IPR003169. GYF.
IPR015943. WD40/YVTN_repeat-like_dom.
PfamiView protein in Pfam
PF02213. GYF. 1 hit.
SMARTiView protein in SMART
SM00444. GYF. 1 hit.
PROSITEiView protein in PROSITE
PS50829. GYF. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6Y7W8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAETQTLNF GPEWLRALSS GGSITSPPLS PALPKYKLAD YRYGREEMLA
60 70 80 90 100
LFLKDYKIPF DLLEKEFLPI LQEEPLPPLA LVPFTEEEQR NFSMSVNSAA
110 120 130 140 150
VLRLTGRGGG GGTVVGAPRG RSSSRGRGRG RGECGFYQRS FDEVEGVFGR
160 170 180 190 200
GGGREMHRSQ SWEERGDRRF EKPGRKDVGR PNFEESGPTS VGRKHEFIRS
210 220 230 240 250
ESENWRIFRE EQNGEDEDGG WRLAGSRRDG ERWRPHSPDG PRSTGWREHM
260 270 280 290 300
ERRRRFEFDF RDRDDERGYR RVRSGSGSID DDRDSLPEWC LEDAEEEMGT
310 320 330 340 350
FDSSGAFLSL KKVQKEPIPE EQEMDFRPVE EGEERSDSDS SHNEEAKEPD
360 370 380 390 400
KTNRREGEKT DRAGAEASEE VPQTSLSSAR PGTPSDHQPQ EATQFERKDE
410 420 430 440 450
PKAEQVEKAE EENRSENSLS AKVPSRGDET VPASQQPSTP LPPDTASPLL
460 470 480 490 500
ILSPPVPTPS SASRPVETAA VEAPGMSSVS TEPDDEEGLK HLEQQAEKMV
510 520 530 540 550
AYLQDSALDD ERLTSKLQEH RAKGVSIPLM HEAMQKWYYK DPQGEIQGPF
560 570 580 590 600
NNQEMAEWFQ AGYFTMSLLV KRACDESFQP LGDIMKMWGR VPFSPGPAPP
610 620 630 640 650
PHMGELDQER LTRQQELTAL YQMQHLQYQQ FLIQQQYAQV LAQQQKAALS
660 670 680 690 700
SQQQQQLALL LQQFQALKMR MSDQNIIPSV TRSVSVPDTG SIWELQPAAS
710 720 730 740 750
QPAVWEGGSV WDLPLDTTAP GPSLEQLQQL EKAKAAKLEQ ERREAEMRAK
760 770 780 790 800
REEEERKRQE ELRRQQEEIL RRQQEEERKR REEEELARRK QEEALRRQRE
810 820 830 840 850
QEIALRRQRE EEERQQQEEA LRRLEERRRE EEERRKQEEL LRKQEEEAAK
860 870 880 890 900
WAREEEEAQR RLEENRLRME EEAARLRHEE EERKRKELEL QRQKDLMRQR
910 920 930 940 950
QQQQEALRRL QQQQQQQQLA QMKLPSSSTW GQQSNTATCQ SQATLSLAEI
960 970 980 990 1000
QKLEEERERQ LREEQRRQQR ELMKALQQQQ QQQQQQKLSG WGNVSKPAGT
1010 1020 1030 1040 1050
TKSLLEIQQE EARQMQKQQQ QQQQQQQQHQ QSNRARNSTH SNLHTSLGNS
1060 1070 1080 1090 1100
VWGSINTGPS NQWASELVSS IWSNADTKNS NMGFWDDAVK EVGPRNSTNK
1110 1120 1130 1140 1150
NKNNASLSKS VGVSNRQNKK VEEEEKLLKL FQGVNKAQDG FTQWCEQMLH
1160 1170 1180 1190 1200
ALNTANNLDV PTFVSFLKEV ESPYEVHDYT RAYLGDTSEA KEFAKQFLER
1210 1220 1230 1240 1250
RAKQKVNQQR QQQQQQQQQQ DSVWGMNHST LHSVFQTNQS NNQQSNFEAV
1260 1270 1280 1290
QSGKKKKKQK MVRADPSLLG FSVNASSERL NMGEIETLDD Y
Length:1,291
Mass (Da):149,193
Last modified:January 9, 2007 - v2
Checksum:iD05938427E72580A
GO
Isoform 2 (identifier: Q6Y7W8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-868: Missing.

Note: No experimental confirmation available.
Show »
Length:423
Mass (Da):49,140
Checksum:iCEE62BEA76486B59
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13E → G in BAE25675 (PubMed:16141072).Curated1
Sequence conflicti549P → S in BAC65619 (PubMed:12693553).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0222471 – 868Missing in isoform 2. 1 PublicationAdd BLAST868

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY176043 mRNA. Translation: AAO46887.1.
BC027137 mRNA. Translation: AAH27137.1.
BC030845 mRNA. Translation: AAH30845.1.
BC082811 mRNA. Translation: AAH82811.1.
BC089036 mRNA. Translation: AAH89036.1.
AK144058 mRNA. Translation: BAE25675.1.
AK122337 mRNA. Translation: BAC65619.1.
CCDSiCCDS15133.1. [Q6Y7W8-1]
RefSeqiNP_001103682.1. NM_001110212.2.
NP_666224.3. NM_146112.4. [Q6Y7W8-1]
XP_006529527.1. XM_006529464.2. [Q6Y7W8-1]
XP_006529528.1. XM_006529465.3. [Q6Y7W8-1]
UniGeneiMm.23065.

Genome annotation databases

EnsembliENSMUST00000027475; ENSMUSP00000027475; ENSMUSG00000048000. [Q6Y7W8-1]
ENSMUST00000174501; ENSMUSP00000133327; ENSMUSG00000048000. [Q6Y7W8-1]
GeneIDi227331.
KEGGimmu:227331.
UCSCiuc007bwt.2. mouse. [Q6Y7W8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGGYF2_MOUSE
AccessioniPrimary (citable) accession number: Q6Y7W8
Secondary accession number(s): Q0VGQ7
, Q3UNS2, Q63ZU9, Q80TV1, Q8K0R0, Q8R0A3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: January 9, 2007
Last modified: June 7, 2017
This is version 97 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families