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Reviewed, UniProtKB/Swiss-Prot Q6Y657 (RIR2B_ARATH)

Last modified October 13, 2009. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Putative ribonucleoside-diphosphate reductase small chain B
    EC=1.17.4.1
Alternative name(s):
    Ribonucleotide reductase small subunit B
    Ribonucleoside-diphosphate reductase R2B subunit
Gene names
Name: RNR2B
Ordered Locus Names: At5g40942
ORF Names: MMG1.4
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidseurosids IIBrassicalesBrassicaceaeArabidopsis

Protein attributes

Sequence length333 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceUncertain.

General annotation (Comments)

Function

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Ref.1

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactor

Binds 2 iron ions per subunit By similarity.

Pathway

Genetic information processing; DNA replication.

Subunit structure

Heterodimer of a large and a small chain By similarity.

Subcellular location

Cytoplasm By similarity.

Tissue specificity

Expressed in roots, rosette leaves, stems and flowers. Ref.1

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase small chain family.

Caution

Could be the product of a pseudogene in cv. Columbia. The N-terminus is shortened due to a frameshift in position 140. The resulting sequence lacks the conserved features of the family.

Sequence caution

The sequence AAO42187.1 differs from that shown. Reason: Frameshift at position 140.

The sequence AB023040 differs from that shown. Reason: Frameshift at position 140.

Ontologies

Keywords
   Biological processDNA replication
   Cellular componentCytoplasm
   LigandIron
Metal-binding
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

deoxyribonucleoside diphosphate metabolic process

Inferred from electronic annotation. Source: InterPro

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioniron ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

ribonucleoside-diphosphate reductase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 333333Putative ribonucleoside-diphosphate reductase small chain B
PRO_0000254194

Sites

Active site1141 By similarity
Metal binding761Iron 1 By similarity
Metal binding1071Iron 1 By similarity
Metal binding1071Iron 2 By similarity
Metal binding1101Iron 1 By similarity
Metal binding1691Iron 2 By similarity
Metal binding2031Iron 2 By similarity
Metal binding2061Iron 2 By similarity

Experimental info

Sequence conflict2211L → F Ref.2
Sequence conflict2211L → F in AAO42187. Ref.3
Sequence conflict2461P → S Ref.2
Sequence conflict2461P → S in AAO42187. Ref.3
Sequence conflict2751A → G Ref.2
Sequence conflict2751A → G in AAO42187. Ref.3
Sequence conflict2811S → T Ref.2
Sequence conflict2811S → T in AAO42187. Ref.3
Sequence conflict3111I → V Ref.2
Sequence conflict3111I → V in AAO42187. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q6Y657-1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: CA20B044F92DB922

FASTA33338,331
        10         20         30         40         50         60 
MPSMPEEPIL TPTPDRFCMF PIQYPQIWEM YKKAEASFWT AEEVDLSQDN RDWENSLTND 

        70         80         90        100        110        120 
ERHFIKHVLA FFAASDGIVL ENLSTRFMSD VQISEARAFY GFQIAIENIH SEMYSLLLDT 

       130        140        150        160        170        180 
YIKDNKERDH LFRAIETIPC VTKKAEWAMK WINGSQSFAE RIVAFACVEG IFFSGSFCSI 

       190        200        210        220        230        240 
FWLKKRGLMP GLTFSNELIS RDEGLHCDFA CLIYSLLRTK LDEDRLKAIV CDAVEIEREF 

       250        260        270        280        290        300 
VCDALPCALV GMNRELMSQY IEFVADRLLA ALGCAKVYGV SNPFDWMELI SLQGKTNFFE 

       310        320        330 
KRVGEYQKAS IMSSVHGNAA FNDDHVFKLD EDF 

« Hide

References

« Hide 'large scale' references
[1]"Arabidopsis ribonucleotide reductases are critical for cell cycle progression, DNA damage repair, and plant development."
Wang C., Liu Z.
Plant Cell 18:350-365(2006) [PubMed: 16399800] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION.
Strain: cv. Landsberg erecta.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. XI."
Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.

Cross-references

Sequence databases

AY178109 mRNA. Translation: AAO62422.1.
AB023040 Genomic DNA. No translation available.
BT004167 mRNA. Translation: AAO42187.1. Frameshift.
IPIIPI00544341.

3D structure databases

HSSPHSSP built from PDB template 1JK0 based on UniProtKB P49723.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ6Y657.

Organism-specific databases

GeneFarm2199. 386.
TAIRAt5g40942.

Enzyme and pathway databases

BRENDA1.17.4.1. 302.
1.17.4.2. 302.

Gene expression databases

GenevestigatorQ6Y657.

Family and domain databases

InterProIPR012348. Ribncl_red_rel.
IPR000358. Ribonucl_redctse.
[Graphical view]
Gene3DG3DSA:1.10.620.20. Ribncl_red_rel. 1 hit.
PANTHERPTHR23409. Ribonucl_redctse. 1 hit.
PfamPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
PROSITEPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRIR2B_ARATH
AccessionPrimary (citable) accession number: Q6Y657
Secondary accession number(s): Q84W71
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: July 5, 2004
Last modified: October 13, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents