Q6Y657 (RIR2B_ARATH) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 64.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Putative ribonucleoside-diphosphate reductase small chain B EC=1.17.4.1 Alternative name(s): Ribonucleoside-diphosphate reductase R2B subunit Ribonucleotide reductase small subunit B | ||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Reference proteome] | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis![]() |
Protein attributes
| Sequence length | 333 AA. |
| Sequence status | Complete. |
| Protein existence | Uncertain |
General annotation (Comments)
| Function | Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Ref.1 |
| Catalytic activity | 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin. |
| Cofactor | Binds 2 iron ions per subunit By similarity. |
| Pathway | |
| Subunit structure | Heterodimer of a large and a small chain By similarity. |
| Subcellular location | Cytoplasm By similarity. |
| Tissue specificity | Expressed in roots, rosette leaves, stems and flowers. Ref.1 |
| Sequence similarities | Belongs to the ribonucleoside diphosphate reductase small chain family. |
| Caution | Could be the product of a pseudogene in cv. Columbia. The N-terminus is shortened due to a frameshift in position 140. The resulting sequence lacks the conserved features of the family. |
| Sequence caution | The sequence AAO42187.1 differs from that shown. Reason: Frameshift at position 140. The sequence AB023040 differs from that shown. Reason: Frameshift at position 140. |
Ontologies
| Keywords | |
|---|---|
| Biological process | DNA replication |
| Cellular component | Cytoplasm |
| Ligand | Iron Metal-binding |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | DNA replication Inferred from electronic annotation. Source: UniProtKB-UniPathway deoxyribonucleoside diphosphate metabolic processInferred from electronic annotation. Source: InterPro |
| Cellular_component | ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro |
| Molecular_function | ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Inferred from electronic annotation. Source: EC transition metal ion bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 333 | 333 | Putative ribonucleoside-diphosphate reductase small chain B | PRO_0000254194 | |||||
Sites | |||||||||
| Active site | 114 | 1 | By similarity | ||||||
| Metal binding | 76 | 1 | Iron 1 By similarity | ||||||
| Metal binding | 107 | 1 | Iron 1 By similarity | ||||||
| Metal binding | 107 | 1 | Iron 2 By similarity | ||||||
| Metal binding | 110 | 1 | Iron 1 By similarity | ||||||
| Metal binding | 169 | 1 | Iron 2 By similarity | ||||||
| Metal binding | 203 | 1 | Iron 2 By similarity | ||||||
| Metal binding | 206 | 1 | Iron 2 By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 221 | 1 | L → F Ref.2 | ||||||
| Sequence conflict | 221 | 1 | L → F in AAO42187. Ref.3 | ||||||
| Sequence conflict | 246 | 1 | P → S Ref.2 | ||||||
| Sequence conflict | 246 | 1 | P → S in AAO42187. Ref.3 | ||||||
| Sequence conflict | 275 | 1 | A → G Ref.2 | ||||||
| Sequence conflict | 275 | 1 | A → G in AAO42187. Ref.3 | ||||||
| Sequence conflict | 281 | 1 | S → T Ref.2 | ||||||
| Sequence conflict | 281 | 1 | S → T in AAO42187. Ref.3 | ||||||
| Sequence conflict | 311 | 1 | I → V Ref.2 | ||||||
| Sequence conflict | 311 | 1 | I → V in AAO42187. Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Arabidopsis ribonucleotide reductases are critical for cell cycle progression, DNA damage repair, and plant development." Wang C., Liu Z. Plant Cell 18:350-365(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION. Strain: cv. Landsberg erecta. |
| [2] | "Structural analysis of Arabidopsis thaliana chromosome 5. XI." Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AY178109 mRNA. Translation: AAO62422.1. AB023040 Genomic DNA. No translation available. BT004167 mRNA. Translation: AAO42187.1. Frameshift. |
| IPI | IPI00544341. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1XSM based on UniProtKB P11157. |
| ProteinModelPortal | Q6Y657. |
| SMR | Q6Y657. Positions 6-287. |
| ModBase | Search... |
Proteomic databases | |
| PaxDb | Q6Y657. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Organism-specific databases | |
| GeneFarm | 2199. 386. |
| TAIR | At5g40942. |
Phylogenomic databases | |
| eggNOG | COG0208. |
| HOGENOM | HOG000255975. |
Enzyme and pathway databases | |
| BRENDA | 1.17.4.2. 399. |
| UniPathway | UPA00326. |
Gene expression databases | |
| ArrayExpress | Q6Y657. |
| Genevestigator | Q6Y657. |
Family and domain databases | |
| Gene3D | 1.10.620.20. 1 hit. |
| InterPro | IPR009078. Ferritin-like_SF. IPR012348. RNR-rel. IPR000358. RNR_small. [Graphical view] |
| PANTHER | PTHR23409. PTHR23409. 1 hit. |
| Pfam | PF00268. Ribonuc_red_sm. 1 hit. [Graphical view] |
| SUPFAM | SSF47240. Ferritin/RR_like. 1 hit. |
| PROSITE | PS00368. RIBORED_SMALL. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | RIR2B_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q6Y657 Secondary accession number(s): Q84W71 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with
