Q6Y5M7 (PMIP_COPSC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial intermediate peptidase
Short name=MIP
EC=3.4.24.59

Alternative name(s):
Octapeptidyl aminopeptidase

Gene names

Name:	OCT1
Synonyms:	MIP

Organism

Coprinopsis scobicola (Ink cap fungus)

Taxonomic identifier

71696 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Agaricomycetes › Agaricomycetidae › Agaricales › Psathyrellaceae › Coprinopsis

Protein attributes

Sequence length	772 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane By similarity.
Catalytic activity	Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.
Cofactor	Binds 1 zinc ion By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the peptidase M3 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 33	33	Mitochondrion Potential
	Chain	34 – 772	739	Mitochondrial intermediate peptidase		PRO_0000343201
Regions
	Compositional bias	485 – 488	4	Poly-Asp
Sites
	Active site	557	1	By similarity
	Metal binding	556	1	Zinc; catalytic By similarity
	Metal binding	560	1	Zinc; catalytic By similarity
	Metal binding	563	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6Y5M7 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 3ACB37CE292B2ED0	FASTA	772	86,698
	10 20 30 40 50 60 MLARPSTTVL ARRPFFRFRG CLNEPRPTKA RCLATAATHH QIPSTVDDKA LVDLFDQPSL 70 80 90 100 110 120 SKVRSHFHST GLFGHPSLTH PRSLVSLAES TLVRAQLLTQ RILDAKESED ELAHVVKNLD 130 140 150 160 170 180 RLSDMLCGVI DLAELVRNAH PDRLWVEAGN HAYETLCEFM NVLNTHTGLN DTLKTVLSNP 190 200 210 220 230 240 TLVKSLDPEA YQTALIFSRD FEKSGIDLPP ATRNKFVSLS SDILILGRQF LENASTPRPP 250 260 270 280 290 300 TSVKASELAG LKDKGMGVRL QLQAQFTNRD LQVYPGSLQA QMIMRAAPNE EPRRKLYLAA 310 320 330 340 350 360 NSSTPEQIHV LETLLKKRAE LAQLVGRDSF AHMTLDDKMA KKPEHVTNFL DALIDHTRPF 370 380 390 400 410 420 ARNALRTLAQ RKQAHHNLPA LPVIQAWDRD FYCPPDPPAP PIPLPPLTIG TVFMGLSRLF 430 440 450 460 470 480 RHLYGVSLRP AQAASGEVWH PDVQKLEVVD EQQGIIGWIY ADLFPRRGKA SGAAHYTVRC 490 500 510 520 530 540 SRRTDDDDEA NDGMFEGTEL QIQESQQFEA VKRHRLPNQE GVYQLPLVVL LTEFARPSLS 550 560 570 580 590 600 KGAAVLEWHE VQTLFHEMGH AMHSMLGRTE YQNVSGTRCA TDFVELPSIL MEHFLNSPAV 610 620 630 640 650 660 LSLFDADNTT SLRQIGNHHN DPCHAIDTYS QIMLAVVDQV YHSPSVLNSS FDSTNEFANL 670 680 690 700 710 720 VNKRGLIPYV PGTSFQTQFG HLFGYGATYY SYLFDRAIAS RVWSKVFSRD PLNRELGEQY 730 740 750 760 770 KQEVLRWGGA RDPWEMVSTL LDQPELAAGD AEAMREVGRW RIEDEVGNSG RH

References

[1]

"Evolution of the gene encoding mitochondrial intermediate peptidase and its cosegregation with the A mating-type locus of mushroom fungi." James T.Y., Kuees U., Rehner S.A., Vilgalys R. Fungal Genet. Biol. 41:381-390(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Cb.M8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY179561 Genomic DNA. Translation: AAO61500.1.
3D structure databases
ProteinModelPortal	Q6Y5M7.
ModBase	Search...
Protein family/group databases
MEROPS	M03.006.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.1370.10. 2 hits. 3.40.390.10. 2 hits.
InterPro	IPR024079. MetalloPept_cat_dom. IPR024077. Neurolysin/TOP_dom2. IPR001567. Pept_M3A_M3B. [Graphical view]
Pfam	PF01432. Peptidase_M3. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMIP_COPSC

Accession

Primary (citable) accession number: Q6Y5M7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 2008
Last sequence update:	July 5, 2004
Last modified:	March 6, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program