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Q6Y5D8 (RHG10_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rho GTPase-activating protein 10
Alternative name(s):
PH and SH3 domain-containing rhoGAP protein
Short name=PS-GAP
Short name=PSGAP
Rho-type GTPase-activating protein 10
Gene names
Name:Arhgap10
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length786 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death. Ref.1 Ref.2

Subunit structure

Interacts with PKN3 By similarity. Interacts with caspase-activated PAK2 proteolytic fragment PAK-2p34; the interaction does not affect ARHGAP10 GTPase activation activity towards RHOA and CDC42. Interacts via its SH3 domain with PTK2/FAK1. Interacts with PTK2B/PYK2; the interaction negatively regulates ARHGAP10 GTPase-activating activity. Ref.1 Ref.2

Subcellular location

Cytoplasm. Cytoplasm › perinuclear region. Cell membrane. Note: Association to cell membrane is dependent on PH domain. Ref.1 Ref.2

Tissue specificity

High levels of expression in brain, testes, liver, heart and kidney. Ref.2

Post-translational modification

Phosphoprylated on tyrosine redidues, probably involving PTK2B/PYK2.

Sequence similarities

Contains 1 BAR domain.

Contains 1 PH domain.

Contains 1 Rho-GAP domain.

Contains 1 SH3 domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PAK2Q295024EBI-4396677,EBI-4406512From a different organism.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6Y5D8-1)

Also known as: PS-GAP-a; PSGAP-m;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6Y5D8-2)

Also known as: PS-GAP-b;

The sequence of this isoform differs from the canonical sequence as follows:
     388-409: Missing.
Isoform 3 (identifier: Q6Y5D8-3)

Also known as: PS-GAP-c;

The sequence of this isoform differs from the canonical sequence as follows:
     677-727: Missing.
Isoform 4 (identifier: Q6Y5D8-4)

Also known as: PS-GAP-s; PSGAP-s;

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 786786Rho GTPase-activating protein 10
PRO_0000304915

Regions

Domain7 – 262256BAR
Domain265 – 372108PH
Domain389 – 574186Rho-GAP
Domain728 – 78659SH3

Amino acid modifications

Modified residue1091Phosphothreonine Ref.3
Modified residue1111Phosphothreonine Ref.3

Natural variations

Alternative sequence1 – 103103Missing in isoform 4.
VSP_028127
Alternative sequence388 – 40922Missing in isoform 2.
VSP_028128
Alternative sequence677 – 72751Missing in isoform 3.
VSP_028129

Experimental info

Mutagenesis4181R → Q: Inactive. Abolishes GTPase activity in vitro. Greatly diminishes cytoskeletal reorganization. Ref.1
Sequence conflict681K → E in AAK18175. Ref.1
Sequence conflict731G → D in AAK18175. Ref.1
Sequence conflict1041M → V in AAO62072. Ref.2
Sequence conflict1361K → E in AAK18175. Ref.1
Sequence conflict1361K → E in AAK18174. Ref.1
Sequence conflict2401E → G in AAK18175. Ref.1
Sequence conflict2401E → G in AAK18174. Ref.1
Sequence conflict3291M → T in AAO62073. Ref.2
Sequence conflict3711E → R in AAK18175. Ref.1
Sequence conflict3711E → R in AAK18174. Ref.1
Sequence conflict3721A → S in AAK18175. Ref.1
Sequence conflict3721A → S in AAK18174. Ref.1
Sequence conflict3731L → S in AAK18175. Ref.1
Sequence conflict3731L → S in AAK18174. Ref.1
Sequence conflict3741F → V in AAK18175. Ref.1
Sequence conflict3741F → V in AAK18174. Ref.1
Sequence conflict3801A → T in AAO62074. Ref.2
Sequence conflict4051A → T in AAO62074. Ref.2
Sequence conflict5701H → R in AAO62074. Ref.2
Sequence conflict6681S → N in AAO62072. Ref.2
Sequence conflict6681S → N in AAO62073. Ref.2
Sequence conflict6681S → N in AAO62074. Ref.2
Sequence conflict7701T → A in AAO62073. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (PS-GAP-a) (PSGAP-m) [UniParc].

Last modified October 2, 2007. Version 2.
Checksum: D55F2E694A153D90

FASTA78689,366
        10         20         30         40         50         60 
MGLQPLEFSD CYLDSPWFRE RIRAHEAELE RTNKFIKELI KDGKNLISAT KSLSAAQRKF 

        70         80         90        100        110        120 
AHSLRDFKFE FIGDAETDDE RCIDASLREF SNFLKNLEEQ REIMALSVTE TLIKPLEKFR 

       130        140        150        160        170        180 
KEQLGAVKEE KKKFDKETEK NYSLIDKHLT LSARKKDSHL QEADLQVEQN RQHFYELSLE 

       190        200        210        220        230        240 
YVCKLQEIQE RKKFEFVEPM LSFFQGMFTF YHQGHELSKD FNHYKMELQI NIQNTRNRFE 

       250        260        270        280        290        300 
GTRSEVEELM NKIRQNPKDQ KRASQFTAEG YLYVQEKRPA PFGSSWVKHY CMYRKTAKKF 

       310        320        330        340        350        360 
NMIPFEHRSG GKLGDGEAFF LKECTKRHMD STDRRFCFDI EAADRPGVPL TVQAFSEEER 

       370        380        390        400        410        420 
KQWLEALGGK EALFHTFNRA IVPRPEGGAQ LDKMGFTILR KCISAVETRG INDQGLYRVV 

       430        440        450        460        470        480 
GVSSKVQRLL SMLMDVKMCN ELDLENSADW EVKTVTSALK QYLRSLPEPL MTYELHRDFI 

       490        500        510        520        530        540 
VPAKSGSPES RVNAIHFLVH KLPEKNKEML DILVKHLTNV SSHSKQNLMT VANLGVVFGP 

       550        560        570        580        590        600 
TLMRPQEETV AAIMDLKFQN IVVEILIENH EKIFRTSPDT TFAEPTCLSA SPPNAPPRQS 

       610        620        630        640        650        660 
KRQGQRTKRP VAVYNLCLEL EEGDSPSPLK EDPPSSSQDS LSTPSPTTSA AHGPPGLDGN 

       670        680        690        700        710        720 
HLAADGGSCG DATATTPSQT RPSMVQWLNM QSPTTPSSNP AGTPPSPRMS PFPLSPAASI 

       730        740        750        760        770        780 
VDKLPECVIN RKARAVYPCE AEHSSELSFE IGAIFEDVQT SREPGWLEGT LNGKRGLIPQ 


NYVKLL

« Hide

Isoform 2 (PS-GAP-b) [UniParc].

Checksum: 4068B92B1766214C
Show »

FASTA76486,946
Isoform 3 (PS-GAP-c) [UniParc].

Checksum: 0457A78BF2389AF5
Show »

FASTA73583,964
Isoform 4 (PS-GAP-s) (PSGAP-s) [UniParc].

Checksum: 02441FEDC2EB0A3B
Show »

FASTA68377,238

References

« Hide 'large scale' references
[1]"Regulation of CDC42 GTPase by proline-rich tyrosine kinase 2 interacting with PSGAP, a novel pleckstrin homology and Src homology 3 domain containing rhoGAP protein."
Ren X.-R., Du Q.-S., Huang Y.-Z., Ao S.-Z., Mei L., Xiong W.-C.
J. Cell Biol. 152:971-984(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 4), FUNCTION, INTERACTION WITH PTK2/FAK1 AND PTK2B/PYK2, SUBCELLULAR LOCATION, PHOSPHORYLATION, MUTAGENESIS OF ARG-418.
Strain: C57BL/10J.
[2]"Identification and characterization of PS-GAP as a novel regulator of caspase-activated PAK-2."
Koeppel M.A., McCarthy C.C., Moertl E., Jakobi R.
J. Biol. Chem. 279:53653-53664(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), FUNCTION, INTERACTION WITH PAK2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Heart.
[3]"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry."
Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R.
J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-109 AND THR-111, MASS SPECTROMETRY.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF297029 mRNA. Translation: AAK18174.1.
AF297030 mRNA. Translation: AAK18175.1.
AY179965 mRNA. Translation: AAO62072.1.
AY179966 mRNA. Translation: AAO62073.1.
AY179967 mRNA. Translation: AAO62074.1.
IPIIPI00118046.
IPI00460449.
IPI00467428.
IPI00867897.
RefSeqNP_084389.2. NM_030113.2.
UniGeneMm.165974.

3D structure databases

HSSPHSSP built from PDB template 1UGV based on UniProtKB Q9UNA1.
ProteinModelPortalQ6Y5D8.
SMRQ6Y5D8. Positions 14-367, 389-579, 731-786.
ModBaseSearch...

Protein-protein interaction databases

IntActQ6Y5D8. 1 interaction.

PTM databases

PhosphoSiteQ6Y5D8.

Proteomic databases

PaxDbQ6Y5D8.
PRIDEQ6Y5D8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000076316; ENSMUSP00000075658; ENSMUSG00000037148.
GeneID78514.
KEGGmmu:78514.
UCSCuc009mhm.1. mouse.
uc009mhn.1. mouse.
uc012gge.1. mouse.

Organism-specific databases

CTD79658.
MGIMGI:1925764. Arhgap10.

Phylogenomic databases

eggNOGNOG311191.
GeneTreeENSGT00700000104048.
HOGENOMHOG000018767.
HOVERGENHBG067993.
InParanoidQ6Y5D8.
KOK13736.
OMATSAMKQY.
OrthoDBEOG49GKG8.

Enzyme and pathway databases

ReactomeREACT_100962. Apoptosis.
REACT_13714. Regulation of PAK-2p34 activity by PS-GAP.

Gene expression databases

ArrayExpressQ6Y5D8.
BgeeQ6Y5D8.
CleanExMM_ARHGAP10.
GenevestigatorQ6Y5D8.

Family and domain databases

Gene3D1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProIPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamPF00620. RhoGAP. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF48350. Rho_GAP. 1 hit.
SSF50044. SH3. 1 hit.
PROSITEPS51021. BAR. False negative.
PS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSARHGAP10. mouse.
NextBio349007.
SOURCESearch...

Entry information

Entry nameRHG10_MOUSE
AccessionPrimary (citable) accession number: Q6Y5D8
Secondary accession number(s): Q6Y5D6 expand/collapse secondary AC list , Q6Y5D7, Q99MT3, Q99MT4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: May 1, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents