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Q6Y5D8

- RHG10_MOUSE

UniProt

Q6Y5D8 - RHG10_MOUSE

Protein

Rho GTPase-activating protein 10

Gene

Arhgap10

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 2 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death.2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. Rho GTPase activator activity Source: MGI

    GO - Biological processi

    1. cytoskeleton organization Source: MGI
    2. negative regulation of apoptotic process Source: UniProtKB
    3. positive regulation of Rho GTPase activity Source: GOC
    4. signal transduction Source: InterPro

    Keywords - Molecular functioni

    GTPase activation

    Enzyme and pathway databases

    ReactomeiREACT_210090. Rho GTPase cycle.
    REACT_223446. Regulation of PAK-2p34 activity by PS-GAP/RHG10.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rho GTPase-activating protein 10
    Alternative name(s):
    PH and SH3 domain-containing rhoGAP protein
    Short name:
    PS-GAP
    Short name:
    PSGAP
    Rho-type GTPase-activating protein 10
    Gene namesi
    Name:Arhgap10
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:1925764. Arhgap10.

    Subcellular locationi

    Cytoplasm. Cytoplasmperinuclear region. Cell membrane
    Note: Association to cell membrane is dependent on PH domain.

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. perinuclear region of cytoplasm Source: UniProtKB-SubCell
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi418 – 4181R → Q: Inactive. Abolishes GTPase activity in vitro. Greatly diminishes cytoskeletal reorganization. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 786786Rho GTPase-activating protein 10PRO_0000304915Add
    BLAST

    Post-translational modificationi

    Phosphoprylated on tyrosine redidues, probably involving PTK2B/PYK2.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ6Y5D8.
    PaxDbiQ6Y5D8.
    PRIDEiQ6Y5D8.

    PTM databases

    PhosphoSiteiQ6Y5D8.

    Expressioni

    Tissue specificityi

    High levels of expression in brain, testes, liver, heart and kidney.1 Publication

    Gene expression databases

    ArrayExpressiQ6Y5D8.
    BgeeiQ6Y5D8.
    CleanExiMM_ARHGAP10.
    GenevestigatoriQ6Y5D8.

    Interactioni

    Subunit structurei

    Interacts with PKN3 By similarity. Interacts with caspase-activated PAK2 proteolytic fragment PAK-2p34; the interaction does not affect ARHGAP10 GTPase activation activity towards RHOA and CDC42. Interacts via its SH3 domain with PTK2/FAK1. Interacts with PTK2B/PYK2; the interaction negatively regulates ARHGAP10 GTPase-activating activity.By similarity2 Publications

    Protein-protein interaction databases

    BioGridi219450. 1 interaction.
    IntActiQ6Y5D8. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6Y5D8.
    SMRiQ6Y5D8. Positions 14-367, 389-579, 730-786.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini7 – 262256BARAdd
    BLAST
    Domaini265 – 372108PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini389 – 574186Rho-GAPPROSITE-ProRule annotationAdd
    BLAST
    Domaini728 – 78659SH3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 BAR domain.Curated
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 Rho-GAP domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH3 domain

    Phylogenomic databases

    eggNOGiNOG311191.
    GeneTreeiENSGT00730000110511.
    HOGENOMiHOG000018767.
    HOVERGENiHBG067993.
    InParanoidiQ6Y5D8.
    KOiK13736.
    OMAiCFDVEAA.
    OrthoDBiEOG71VSS5.
    PhylomeDBiQ6Y5D8.
    TreeFamiTF316851.

    Family and domain databases

    Gene3Di1.10.555.10. 1 hit.
    2.30.29.30. 1 hit.
    InterProiIPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR008936. Rho_GTPase_activation_prot.
    IPR000198. RhoGAP_dom.
    IPR001452. SH3_domain.
    [Graphical view]
    PfamiPF00620. RhoGAP. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view]
    SMARTiSM00233. PH. 1 hit.
    SM00324. RhoGAP. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF48350. SSF48350. 1 hit.
    SSF50044. SSF50044. 1 hit.
    PROSITEiPS50003. PH_DOMAIN. 1 hit.
    PS50238. RHOGAP. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6Y5D8-1) [UniParc]FASTAAdd to Basket

    Also known as: PS-GAP-a, PSGAP-m

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGLQPLEFSD CYLDSPWFRE RIRAHEAELE RTNKFIKELI KDGKNLISAT    50
    KSLSAAQRKF AHSLRDFKFE FIGDAETDDE RCIDASLREF SNFLKNLEEQ 100
    REIMALSVTE TLIKPLEKFR KEQLGAVKEE KKKFDKETEK NYSLIDKHLT 150
    LSARKKDSHL QEADLQVEQN RQHFYELSLE YVCKLQEIQE RKKFEFVEPM 200
    LSFFQGMFTF YHQGHELSKD FNHYKMELQI NIQNTRNRFE GTRSEVEELM 250
    NKIRQNPKDQ KRASQFTAEG YLYVQEKRPA PFGSSWVKHY CMYRKTAKKF 300
    NMIPFEHRSG GKLGDGEAFF LKECTKRHMD STDRRFCFDI EAADRPGVPL 350
    TVQAFSEEER KQWLEALGGK EALFHTFNRA IVPRPEGGAQ LDKMGFTILR 400
    KCISAVETRG INDQGLYRVV GVSSKVQRLL SMLMDVKMCN ELDLENSADW 450
    EVKTVTSALK QYLRSLPEPL MTYELHRDFI VPAKSGSPES RVNAIHFLVH 500
    KLPEKNKEML DILVKHLTNV SSHSKQNLMT VANLGVVFGP TLMRPQEETV 550
    AAIMDLKFQN IVVEILIENH EKIFRTSPDT TFAEPTCLSA SPPNAPPRQS 600
    KRQGQRTKRP VAVYNLCLEL EEGDSPSPLK EDPPSSSQDS LSTPSPTTSA 650
    AHGPPGLDGN HLAADGGSCG DATATTPSQT RPSMVQWLNM QSPTTPSSNP 700
    AGTPPSPRMS PFPLSPAASI VDKLPECVIN RKARAVYPCE AEHSSELSFE 750
    IGAIFEDVQT SREPGWLEGT LNGKRGLIPQ NYVKLL 786
    Length:786
    Mass (Da):89,366
    Last modified:October 2, 2007 - v2
    Checksum:iD55F2E694A153D90
    GO
    Isoform 2 (identifier: Q6Y5D8-2) [UniParc]FASTAAdd to Basket

    Also known as: PS-GAP-b

    The sequence of this isoform differs from the canonical sequence as follows:
         388-409: Missing.

    Show »
    Length:764
    Mass (Da):86,946
    Checksum:i4068B92B1766214C
    GO
    Isoform 3 (identifier: Q6Y5D8-3) [UniParc]FASTAAdd to Basket

    Also known as: PS-GAP-c

    The sequence of this isoform differs from the canonical sequence as follows:
         677-727: Missing.

    Show »
    Length:735
    Mass (Da):83,964
    Checksum:i0457A78BF2389AF5
    GO
    Isoform 4 (identifier: Q6Y5D8-4) [UniParc]FASTAAdd to Basket

    Also known as: PS-GAP-s, PSGAP-s

    The sequence of this isoform differs from the canonical sequence as follows:
         1-103: Missing.

    Show »
    Length:683
    Mass (Da):77,238
    Checksum:i02441FEDC2EB0A3B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti68 – 681K → E in AAK18175. (PubMed:11238453)Curated
    Sequence conflicti73 – 731G → D in AAK18175. (PubMed:11238453)Curated
    Sequence conflicti104 – 1041M → V in AAO62072. (PubMed:15471851)Curated
    Sequence conflicti136 – 1361K → E in AAK18175. (PubMed:11238453)Curated
    Sequence conflicti136 – 1361K → E in AAK18174. (PubMed:11238453)Curated
    Sequence conflicti240 – 2401E → G in AAK18175. (PubMed:11238453)Curated
    Sequence conflicti240 – 2401E → G in AAK18174. (PubMed:11238453)Curated
    Sequence conflicti329 – 3291M → T in AAO62073. (PubMed:15471851)Curated
    Sequence conflicti371 – 3711E → R in AAK18175. (PubMed:11238453)Curated
    Sequence conflicti371 – 3711E → R in AAK18174. (PubMed:11238453)Curated
    Sequence conflicti372 – 3721A → S in AAK18175. (PubMed:11238453)Curated
    Sequence conflicti372 – 3721A → S in AAK18174. (PubMed:11238453)Curated
    Sequence conflicti373 – 3731L → S in AAK18175. (PubMed:11238453)Curated
    Sequence conflicti373 – 3731L → S in AAK18174. (PubMed:11238453)Curated
    Sequence conflicti374 – 3741F → V in AAK18175. (PubMed:11238453)Curated
    Sequence conflicti374 – 3741F → V in AAK18174. (PubMed:11238453)Curated
    Sequence conflicti380 – 3801A → T in AAO62074. (PubMed:15471851)Curated
    Sequence conflicti405 – 4051A → T in AAO62074. (PubMed:15471851)Curated
    Sequence conflicti570 – 5701H → R in AAO62074. (PubMed:15471851)Curated
    Sequence conflicti668 – 6681S → N in AAO62072. (PubMed:15471851)Curated
    Sequence conflicti668 – 6681S → N in AAO62073. (PubMed:15471851)Curated
    Sequence conflicti668 – 6681S → N in AAO62074. (PubMed:15471851)Curated
    Sequence conflicti770 – 7701T → A in AAO62073. (PubMed:15471851)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 103103Missing in isoform 4. 1 PublicationVSP_028127Add
    BLAST
    Alternative sequencei388 – 40922Missing in isoform 2. 1 PublicationVSP_028128Add
    BLAST
    Alternative sequencei677 – 72751Missing in isoform 3. 1 PublicationVSP_028129Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF297029 mRNA. Translation: AAK18174.1.
    AF297030 mRNA. Translation: AAK18175.1.
    AY179965 mRNA. Translation: AAO62072.1.
    AY179966 mRNA. Translation: AAO62073.1.
    AY179967 mRNA. Translation: AAO62074.1.
    CCDSiCCDS22425.1. [Q6Y5D8-1]
    RefSeqiNP_084389.2. NM_030113.2. [Q6Y5D8-1]
    XP_006531558.1. XM_006531495.1. [Q6Y5D8-2]
    XP_006531561.1. XM_006531498.1. [Q6Y5D8-3]
    XP_006531562.1. XM_006531499.1. [Q6Y5D8-4]
    XP_006531563.1. XM_006531500.1. [Q6Y5D8-4]
    UniGeneiMm.165974.

    Genome annotation databases

    EnsembliENSMUST00000076316; ENSMUSP00000075658; ENSMUSG00000037148. [Q6Y5D8-1]
    GeneIDi78514.
    KEGGimmu:78514.
    UCSCiuc009mhm.1. mouse. [Q6Y5D8-1]
    uc009mhn.1. mouse. [Q6Y5D8-3]
    uc012gge.1. mouse. [Q6Y5D8-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF297029 mRNA. Translation: AAK18174.1 .
    AF297030 mRNA. Translation: AAK18175.1 .
    AY179965 mRNA. Translation: AAO62072.1 .
    AY179966 mRNA. Translation: AAO62073.1 .
    AY179967 mRNA. Translation: AAO62074.1 .
    CCDSi CCDS22425.1. [Q6Y5D8-1 ]
    RefSeqi NP_084389.2. NM_030113.2. [Q6Y5D8-1 ]
    XP_006531558.1. XM_006531495.1. [Q6Y5D8-2 ]
    XP_006531561.1. XM_006531498.1. [Q6Y5D8-3 ]
    XP_006531562.1. XM_006531499.1. [Q6Y5D8-4 ]
    XP_006531563.1. XM_006531500.1. [Q6Y5D8-4 ]
    UniGenei Mm.165974.

    3D structure databases

    ProteinModelPortali Q6Y5D8.
    SMRi Q6Y5D8. Positions 14-367, 389-579, 730-786.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 219450. 1 interaction.
    IntActi Q6Y5D8. 1 interaction.

    PTM databases

    PhosphoSitei Q6Y5D8.

    Proteomic databases

    MaxQBi Q6Y5D8.
    PaxDbi Q6Y5D8.
    PRIDEi Q6Y5D8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000076316 ; ENSMUSP00000075658 ; ENSMUSG00000037148 . [Q6Y5D8-1 ]
    GeneIDi 78514.
    KEGGi mmu:78514.
    UCSCi uc009mhm.1. mouse. [Q6Y5D8-1 ]
    uc009mhn.1. mouse. [Q6Y5D8-3 ]
    uc012gge.1. mouse. [Q6Y5D8-2 ]

    Organism-specific databases

    CTDi 79658.
    MGIi MGI:1925764. Arhgap10.

    Phylogenomic databases

    eggNOGi NOG311191.
    GeneTreei ENSGT00730000110511.
    HOGENOMi HOG000018767.
    HOVERGENi HBG067993.
    InParanoidi Q6Y5D8.
    KOi K13736.
    OMAi CFDVEAA.
    OrthoDBi EOG71VSS5.
    PhylomeDBi Q6Y5D8.
    TreeFami TF316851.

    Enzyme and pathway databases

    Reactomei REACT_210090. Rho GTPase cycle.
    REACT_223446. Regulation of PAK-2p34 activity by PS-GAP/RHG10.

    Miscellaneous databases

    ChiTaRSi ARHGAP10. mouse.
    NextBioi 349007.
    PROi Q6Y5D8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q6Y5D8.
    Bgeei Q6Y5D8.
    CleanExi MM_ARHGAP10.
    Genevestigatori Q6Y5D8.

    Family and domain databases

    Gene3Di 1.10.555.10. 1 hit.
    2.30.29.30. 1 hit.
    InterProi IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR008936. Rho_GTPase_activation_prot.
    IPR000198. RhoGAP_dom.
    IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF00620. RhoGAP. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view ]
    SMARTi SM00233. PH. 1 hit.
    SM00324. RhoGAP. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48350. SSF48350. 1 hit.
    SSF50044. SSF50044. 1 hit.
    PROSITEi PS50003. PH_DOMAIN. 1 hit.
    PS50238. RHOGAP. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Regulation of CDC42 GTPase by proline-rich tyrosine kinase 2 interacting with PSGAP, a novel pleckstrin homology and Src homology 3 domain containing rhoGAP protein."
      Ren X.-R., Du Q.-S., Huang Y.-Z., Ao S.-Z., Mei L., Xiong W.-C.
      J. Cell Biol. 152:971-984(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 4), FUNCTION, INTERACTION WITH PTK2/FAK1 AND PTK2B/PYK2, SUBCELLULAR LOCATION, PHOSPHORYLATION, MUTAGENESIS OF ARG-418.
      Strain: C57BL/10J.
    2. "Identification and characterization of PS-GAP as a novel regulator of caspase-activated PAK-2."
      Koeppel M.A., McCarthy C.C., Moertl E., Jakobi R.
      J. Biol. Chem. 279:53653-53664(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), FUNCTION, INTERACTION WITH PAK2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: BALB/c.
      Tissue: Heart.

    Entry informationi

    Entry nameiRHG10_MOUSE
    AccessioniPrimary (citable) accession number: Q6Y5D8
    Secondary accession number(s): Q6Y5D6
    , Q6Y5D7, Q99MT3, Q99MT4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 90 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3