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Q6Y5D8

- RHG10_MOUSE

UniProt

Q6Y5D8 - RHG10_MOUSE

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Protein

Rho GTPase-activating protein 10

Gene
Arhgap10
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death.2 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. Rho GTPase activator activity Source: MGI

GO - Biological processi

  1. cytoskeleton organization Source: MGI
  2. negative regulation of apoptotic process Source: UniProtKB
  3. positive regulation of Rho GTPase activity Source: GOC
  4. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiREACT_210090. Rho GTPase cycle.
REACT_223446. Regulation of PAK-2p34 activity by PS-GAP/RHG10.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 10
Alternative name(s):
PH and SH3 domain-containing rhoGAP protein
Short name:
PS-GAP
Short name:
PSGAP
Rho-type GTPase-activating protein 10
Gene namesi
Name:Arhgap10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1925764. Arhgap10.

Subcellular locationi

Cytoplasm. Cytoplasmperinuclear region. Cell membrane
Note: Association to cell membrane is dependent on PH domain.2 Publications

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. perinuclear region of cytoplasm Source: UniProtKB-SubCell
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi418 – 4181R → Q: Inactive. Abolishes GTPase activity in vitro. Greatly diminishes cytoskeletal reorganization. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 786786Rho GTPase-activating protein 10PRO_0000304915Add
BLAST

Post-translational modificationi

Phosphoprylated on tyrosine redidues, probably involving PTK2B/PYK2.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6Y5D8.
PaxDbiQ6Y5D8.
PRIDEiQ6Y5D8.

PTM databases

PhosphoSiteiQ6Y5D8.

Expressioni

Tissue specificityi

High levels of expression in brain, testes, liver, heart and kidney.1 Publication

Gene expression databases

ArrayExpressiQ6Y5D8.
BgeeiQ6Y5D8.
CleanExiMM_ARHGAP10.
GenevestigatoriQ6Y5D8.

Interactioni

Subunit structurei

Interacts with PKN3 By similarity. Interacts with caspase-activated PAK2 proteolytic fragment PAK-2p34; the interaction does not affect ARHGAP10 GTPase activation activity towards RHOA and CDC42. Interacts via its SH3 domain with PTK2/FAK1. Interacts with PTK2B/PYK2; the interaction negatively regulates ARHGAP10 GTPase-activating activity.2 Publications

Protein-protein interaction databases

BioGridi219450. 1 interaction.
IntActiQ6Y5D8. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ6Y5D8.
SMRiQ6Y5D8. Positions 14-367, 389-579, 730-786.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 262256BARAdd
BLAST
Domaini265 – 372108PHAdd
BLAST
Domaini389 – 574186Rho-GAPAdd
BLAST
Domaini728 – 78659SH3Add
BLAST

Sequence similaritiesi

Contains 1 BAR domain.
Contains 1 PH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain.

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiNOG311191.
GeneTreeiENSGT00730000110511.
HOGENOMiHOG000018767.
HOVERGENiHBG067993.
InParanoidiQ6Y5D8.
KOiK13736.
OMAiCFDVEAA.
OrthoDBiEOG71VSS5.
PhylomeDBiQ6Y5D8.
TreeFamiTF316851.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00620. RhoGAP. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6Y5D8-1) [UniParc]FASTAAdd to Basket

Also known as: PS-GAP-a, PSGAP-m

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGLQPLEFSD CYLDSPWFRE RIRAHEAELE RTNKFIKELI KDGKNLISAT    50
KSLSAAQRKF AHSLRDFKFE FIGDAETDDE RCIDASLREF SNFLKNLEEQ 100
REIMALSVTE TLIKPLEKFR KEQLGAVKEE KKKFDKETEK NYSLIDKHLT 150
LSARKKDSHL QEADLQVEQN RQHFYELSLE YVCKLQEIQE RKKFEFVEPM 200
LSFFQGMFTF YHQGHELSKD FNHYKMELQI NIQNTRNRFE GTRSEVEELM 250
NKIRQNPKDQ KRASQFTAEG YLYVQEKRPA PFGSSWVKHY CMYRKTAKKF 300
NMIPFEHRSG GKLGDGEAFF LKECTKRHMD STDRRFCFDI EAADRPGVPL 350
TVQAFSEEER KQWLEALGGK EALFHTFNRA IVPRPEGGAQ LDKMGFTILR 400
KCISAVETRG INDQGLYRVV GVSSKVQRLL SMLMDVKMCN ELDLENSADW 450
EVKTVTSALK QYLRSLPEPL MTYELHRDFI VPAKSGSPES RVNAIHFLVH 500
KLPEKNKEML DILVKHLTNV SSHSKQNLMT VANLGVVFGP TLMRPQEETV 550
AAIMDLKFQN IVVEILIENH EKIFRTSPDT TFAEPTCLSA SPPNAPPRQS 600
KRQGQRTKRP VAVYNLCLEL EEGDSPSPLK EDPPSSSQDS LSTPSPTTSA 650
AHGPPGLDGN HLAADGGSCG DATATTPSQT RPSMVQWLNM QSPTTPSSNP 700
AGTPPSPRMS PFPLSPAASI VDKLPECVIN RKARAVYPCE AEHSSELSFE 750
IGAIFEDVQT SREPGWLEGT LNGKRGLIPQ NYVKLL 786
Length:786
Mass (Da):89,366
Last modified:October 2, 2007 - v2
Checksum:iD55F2E694A153D90
GO
Isoform 2 (identifier: Q6Y5D8-2) [UniParc]FASTAAdd to Basket

Also known as: PS-GAP-b

The sequence of this isoform differs from the canonical sequence as follows:
     388-409: Missing.

Show »
Length:764
Mass (Da):86,946
Checksum:i4068B92B1766214C
GO
Isoform 3 (identifier: Q6Y5D8-3) [UniParc]FASTAAdd to Basket

Also known as: PS-GAP-c

The sequence of this isoform differs from the canonical sequence as follows:
     677-727: Missing.

Show »
Length:735
Mass (Da):83,964
Checksum:i0457A78BF2389AF5
GO
Isoform 4 (identifier: Q6Y5D8-4) [UniParc]FASTAAdd to Basket

Also known as: PS-GAP-s, PSGAP-s

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.

Show »
Length:683
Mass (Da):77,238
Checksum:i02441FEDC2EB0A3B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 103103Missing in isoform 4. VSP_028127Add
BLAST
Alternative sequencei388 – 40922Missing in isoform 2. VSP_028128Add
BLAST
Alternative sequencei677 – 72751Missing in isoform 3. VSP_028129Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681K → E in AAK18175. 1 Publication
Sequence conflicti73 – 731G → D in AAK18175. 1 Publication
Sequence conflicti104 – 1041M → V in AAO62072. 1 Publication
Sequence conflicti136 – 1361K → E in AAK18175. 1 Publication
Sequence conflicti136 – 1361K → E in AAK18174. 1 Publication
Sequence conflicti240 – 2401E → G in AAK18175. 1 Publication
Sequence conflicti240 – 2401E → G in AAK18174. 1 Publication
Sequence conflicti329 – 3291M → T in AAO62073. 1 Publication
Sequence conflicti371 – 3711E → R in AAK18175. 1 Publication
Sequence conflicti371 – 3711E → R in AAK18174. 1 Publication
Sequence conflicti372 – 3721A → S in AAK18175. 1 Publication
Sequence conflicti372 – 3721A → S in AAK18174. 1 Publication
Sequence conflicti373 – 3731L → S in AAK18175. 1 Publication
Sequence conflicti373 – 3731L → S in AAK18174. 1 Publication
Sequence conflicti374 – 3741F → V in AAK18175. 1 Publication
Sequence conflicti374 – 3741F → V in AAK18174. 1 Publication
Sequence conflicti380 – 3801A → T in AAO62074. 1 Publication
Sequence conflicti405 – 4051A → T in AAO62074. 1 Publication
Sequence conflicti570 – 5701H → R in AAO62074. 1 Publication
Sequence conflicti668 – 6681S → N in AAO62072. 1 Publication
Sequence conflicti668 – 6681S → N in AAO62073. 1 Publication
Sequence conflicti668 – 6681S → N in AAO62074. 1 Publication
Sequence conflicti770 – 7701T → A in AAO62073. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF297029 mRNA. Translation: AAK18174.1.
AF297030 mRNA. Translation: AAK18175.1.
AY179965 mRNA. Translation: AAO62072.1.
AY179966 mRNA. Translation: AAO62073.1.
AY179967 mRNA. Translation: AAO62074.1.
CCDSiCCDS22425.1. [Q6Y5D8-1]
RefSeqiNP_084389.2. NM_030113.2. [Q6Y5D8-1]
XP_006531558.1. XM_006531495.1. [Q6Y5D8-2]
XP_006531561.1. XM_006531498.1. [Q6Y5D8-3]
XP_006531562.1. XM_006531499.1. [Q6Y5D8-4]
XP_006531563.1. XM_006531500.1. [Q6Y5D8-4]
UniGeneiMm.165974.

Genome annotation databases

EnsembliENSMUST00000076316; ENSMUSP00000075658; ENSMUSG00000037148. [Q6Y5D8-1]
GeneIDi78514.
KEGGimmu:78514.
UCSCiuc009mhm.1. mouse. [Q6Y5D8-1]
uc009mhn.1. mouse. [Q6Y5D8-3]
uc012gge.1. mouse. [Q6Y5D8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF297029 mRNA. Translation: AAK18174.1 .
AF297030 mRNA. Translation: AAK18175.1 .
AY179965 mRNA. Translation: AAO62072.1 .
AY179966 mRNA. Translation: AAO62073.1 .
AY179967 mRNA. Translation: AAO62074.1 .
CCDSi CCDS22425.1. [Q6Y5D8-1 ]
RefSeqi NP_084389.2. NM_030113.2. [Q6Y5D8-1 ]
XP_006531558.1. XM_006531495.1. [Q6Y5D8-2 ]
XP_006531561.1. XM_006531498.1. [Q6Y5D8-3 ]
XP_006531562.1. XM_006531499.1. [Q6Y5D8-4 ]
XP_006531563.1. XM_006531500.1. [Q6Y5D8-4 ]
UniGenei Mm.165974.

3D structure databases

ProteinModelPortali Q6Y5D8.
SMRi Q6Y5D8. Positions 14-367, 389-579, 730-786.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 219450. 1 interaction.
IntActi Q6Y5D8. 1 interaction.

PTM databases

PhosphoSitei Q6Y5D8.

Proteomic databases

MaxQBi Q6Y5D8.
PaxDbi Q6Y5D8.
PRIDEi Q6Y5D8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000076316 ; ENSMUSP00000075658 ; ENSMUSG00000037148 . [Q6Y5D8-1 ]
GeneIDi 78514.
KEGGi mmu:78514.
UCSCi uc009mhm.1. mouse. [Q6Y5D8-1 ]
uc009mhn.1. mouse. [Q6Y5D8-3 ]
uc012gge.1. mouse. [Q6Y5D8-2 ]

Organism-specific databases

CTDi 79658.
MGIi MGI:1925764. Arhgap10.

Phylogenomic databases

eggNOGi NOG311191.
GeneTreei ENSGT00730000110511.
HOGENOMi HOG000018767.
HOVERGENi HBG067993.
InParanoidi Q6Y5D8.
KOi K13736.
OMAi CFDVEAA.
OrthoDBi EOG71VSS5.
PhylomeDBi Q6Y5D8.
TreeFami TF316851.

Enzyme and pathway databases

Reactomei REACT_210090. Rho GTPase cycle.
REACT_223446. Regulation of PAK-2p34 activity by PS-GAP/RHG10.

Miscellaneous databases

ChiTaRSi ARHGAP10. mouse.
NextBioi 349007.
PROi Q6Y5D8.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q6Y5D8.
Bgeei Q6Y5D8.
CleanExi MM_ARHGAP10.
Genevestigatori Q6Y5D8.

Family and domain databases

Gene3Di 1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProi IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
[Graphical view ]
Pfami PF00620. RhoGAP. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view ]
SMARTi SM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEi PS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Regulation of CDC42 GTPase by proline-rich tyrosine kinase 2 interacting with PSGAP, a novel pleckstrin homology and Src homology 3 domain containing rhoGAP protein."
    Ren X.-R., Du Q.-S., Huang Y.-Z., Ao S.-Z., Mei L., Xiong W.-C.
    J. Cell Biol. 152:971-984(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 4), FUNCTION, INTERACTION WITH PTK2/FAK1 AND PTK2B/PYK2, SUBCELLULAR LOCATION, PHOSPHORYLATION, MUTAGENESIS OF ARG-418.
    Strain: C57BL/10J.
  2. "Identification and characterization of PS-GAP as a novel regulator of caspase-activated PAK-2."
    Koeppel M.A., McCarthy C.C., Moertl E., Jakobi R.
    J. Biol. Chem. 279:53653-53664(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), FUNCTION, INTERACTION WITH PAK2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: BALB/c.
    Tissue: Heart.

Entry informationi

Entry nameiRHG10_MOUSE
AccessioniPrimary (citable) accession number: Q6Y5D8
Secondary accession number(s): Q6Y5D6
, Q6Y5D7, Q99MT3, Q99MT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: September 3, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi