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Protein

Rho GTPase-activating protein 10

Gene

Arhgap10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death.2 Publications

GO - Molecular functioni

  1. Rho GTPase activator activity Source: MGI

GO - Biological processi

  1. cytoskeleton organization Source: MGI
  2. negative regulation of apoptotic process Source: UniProtKB
  3. positive regulation of Rho GTPase activity Source: GOC
  4. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiREACT_297947. Rho GTPase cycle.
REACT_322416. Regulation of PAK-2p34 activity by PS-GAP/RHG10.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 10
Alternative name(s):
PH and SH3 domain-containing rhoGAP protein
Short name:
PS-GAP
Short name:
PSGAP
Rho-type GTPase-activating protein 10
Gene namesi
Name:Arhgap10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1925764. Arhgap10.

Subcellular locationi

Cytoplasm. Cytoplasmperinuclear region. Cell membrane
Note: Association to cell membrane is dependent on PH domain.

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. perinuclear region of cytoplasm Source: UniProtKB-SubCell
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi418 – 4181R → Q: Inactive. Abolishes GTPase activity in vitro. Greatly diminishes cytoskeletal reorganization. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 786786Rho GTPase-activating protein 10PRO_0000304915Add
BLAST

Post-translational modificationi

Phosphoprylated on tyrosine redidues, probably involving PTK2B/PYK2.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6Y5D8.
PaxDbiQ6Y5D8.
PRIDEiQ6Y5D8.

PTM databases

PhosphoSiteiQ6Y5D8.

Expressioni

Tissue specificityi

High levels of expression in brain, testes, liver, heart and kidney.1 Publication

Gene expression databases

BgeeiQ6Y5D8.
CleanExiMM_ARHGAP10.
ExpressionAtlasiQ6Y5D8. baseline and differential.
GenevestigatoriQ6Y5D8.

Interactioni

Subunit structurei

Interacts with PKN3 (By similarity). Interacts with caspase-activated PAK2 proteolytic fragment PAK-2p34; the interaction does not affect ARHGAP10 GTPase activation activity towards RHOA and CDC42. Interacts via its SH3 domain with PTK2/FAK1. Interacts with PTK2B/PYK2; the interaction negatively regulates ARHGAP10 GTPase-activating activity.By similarity2 Publications

Protein-protein interaction databases

BioGridi219450. 1 interaction.
IntActiQ6Y5D8. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ6Y5D8.
SMRiQ6Y5D8. Positions 14-367, 389-579, 730-786.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 262256BARAdd
BLAST
Domaini265 – 372108PHPROSITE-ProRule annotationAdd
BLAST
Domaini389 – 574186Rho-GAPPROSITE-ProRule annotationAdd
BLAST
Domaini728 – 78659SH3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 BAR domain.Curated
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiNOG311191.
GeneTreeiENSGT00780000121833.
HOGENOMiHOG000018767.
HOVERGENiHBG067993.
InParanoidiQ6Y5D8.
KOiK13736.
OMAiCFDVEAA.
OrthoDBiEOG71VSS5.
PhylomeDBiQ6Y5D8.
TreeFamiTF316851.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00620. RhoGAP. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6Y5D8-1) [UniParc]FASTAAdd to basket

Also known as: PS-GAP-a, PSGAP-m

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGLQPLEFSD CYLDSPWFRE RIRAHEAELE RTNKFIKELI KDGKNLISAT
60 70 80 90 100
KSLSAAQRKF AHSLRDFKFE FIGDAETDDE RCIDASLREF SNFLKNLEEQ
110 120 130 140 150
REIMALSVTE TLIKPLEKFR KEQLGAVKEE KKKFDKETEK NYSLIDKHLT
160 170 180 190 200
LSARKKDSHL QEADLQVEQN RQHFYELSLE YVCKLQEIQE RKKFEFVEPM
210 220 230 240 250
LSFFQGMFTF YHQGHELSKD FNHYKMELQI NIQNTRNRFE GTRSEVEELM
260 270 280 290 300
NKIRQNPKDQ KRASQFTAEG YLYVQEKRPA PFGSSWVKHY CMYRKTAKKF
310 320 330 340 350
NMIPFEHRSG GKLGDGEAFF LKECTKRHMD STDRRFCFDI EAADRPGVPL
360 370 380 390 400
TVQAFSEEER KQWLEALGGK EALFHTFNRA IVPRPEGGAQ LDKMGFTILR
410 420 430 440 450
KCISAVETRG INDQGLYRVV GVSSKVQRLL SMLMDVKMCN ELDLENSADW
460 470 480 490 500
EVKTVTSALK QYLRSLPEPL MTYELHRDFI VPAKSGSPES RVNAIHFLVH
510 520 530 540 550
KLPEKNKEML DILVKHLTNV SSHSKQNLMT VANLGVVFGP TLMRPQEETV
560 570 580 590 600
AAIMDLKFQN IVVEILIENH EKIFRTSPDT TFAEPTCLSA SPPNAPPRQS
610 620 630 640 650
KRQGQRTKRP VAVYNLCLEL EEGDSPSPLK EDPPSSSQDS LSTPSPTTSA
660 670 680 690 700
AHGPPGLDGN HLAADGGSCG DATATTPSQT RPSMVQWLNM QSPTTPSSNP
710 720 730 740 750
AGTPPSPRMS PFPLSPAASI VDKLPECVIN RKARAVYPCE AEHSSELSFE
760 770 780
IGAIFEDVQT SREPGWLEGT LNGKRGLIPQ NYVKLL
Length:786
Mass (Da):89,366
Last modified:October 1, 2007 - v2
Checksum:iD55F2E694A153D90
GO
Isoform 2 (identifier: Q6Y5D8-2) [UniParc]FASTAAdd to basket

Also known as: PS-GAP-b

The sequence of this isoform differs from the canonical sequence as follows:
     388-409: Missing.

Show »
Length:764
Mass (Da):86,946
Checksum:i4068B92B1766214C
GO
Isoform 3 (identifier: Q6Y5D8-3) [UniParc]FASTAAdd to basket

Also known as: PS-GAP-c

The sequence of this isoform differs from the canonical sequence as follows:
     677-727: Missing.

Show »
Length:735
Mass (Da):83,964
Checksum:i0457A78BF2389AF5
GO
Isoform 4 (identifier: Q6Y5D8-4) [UniParc]FASTAAdd to basket

Also known as: PS-GAP-s, PSGAP-s

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.

Show »
Length:683
Mass (Da):77,238
Checksum:i02441FEDC2EB0A3B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681K → E in AAK18175 (PubMed:11238453).Curated
Sequence conflicti73 – 731G → D in AAK18175 (PubMed:11238453).Curated
Sequence conflicti104 – 1041M → V in AAO62072 (PubMed:15471851).Curated
Sequence conflicti136 – 1361K → E in AAK18175 (PubMed:11238453).Curated
Sequence conflicti136 – 1361K → E in AAK18174 (PubMed:11238453).Curated
Sequence conflicti240 – 2401E → G in AAK18175 (PubMed:11238453).Curated
Sequence conflicti240 – 2401E → G in AAK18174 (PubMed:11238453).Curated
Sequence conflicti329 – 3291M → T in AAO62073 (PubMed:15471851).Curated
Sequence conflicti371 – 3711E → R in AAK18175 (PubMed:11238453).Curated
Sequence conflicti371 – 3711E → R in AAK18174 (PubMed:11238453).Curated
Sequence conflicti372 – 3721A → S in AAK18175 (PubMed:11238453).Curated
Sequence conflicti372 – 3721A → S in AAK18174 (PubMed:11238453).Curated
Sequence conflicti373 – 3731L → S in AAK18175 (PubMed:11238453).Curated
Sequence conflicti373 – 3731L → S in AAK18174 (PubMed:11238453).Curated
Sequence conflicti374 – 3741F → V in AAK18175 (PubMed:11238453).Curated
Sequence conflicti374 – 3741F → V in AAK18174 (PubMed:11238453).Curated
Sequence conflicti380 – 3801A → T in AAO62074 (PubMed:15471851).Curated
Sequence conflicti405 – 4051A → T in AAO62074 (PubMed:15471851).Curated
Sequence conflicti570 – 5701H → R in AAO62074 (PubMed:15471851).Curated
Sequence conflicti668 – 6681S → N in AAO62072 (PubMed:15471851).Curated
Sequence conflicti668 – 6681S → N in AAO62073 (PubMed:15471851).Curated
Sequence conflicti668 – 6681S → N in AAO62074 (PubMed:15471851).Curated
Sequence conflicti770 – 7701T → A in AAO62073 (PubMed:15471851).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 103103Missing in isoform 4. 1 PublicationVSP_028127Add
BLAST
Alternative sequencei388 – 40922Missing in isoform 2. 1 PublicationVSP_028128Add
BLAST
Alternative sequencei677 – 72751Missing in isoform 3. 1 PublicationVSP_028129Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF297029 mRNA. Translation: AAK18174.1.
AF297030 mRNA. Translation: AAK18175.1.
AY179965 mRNA. Translation: AAO62072.1.
AY179966 mRNA. Translation: AAO62073.1.
AY179967 mRNA. Translation: AAO62074.1.
CCDSiCCDS22425.1. [Q6Y5D8-1]
RefSeqiNP_084389.2. NM_030113.2. [Q6Y5D8-1]
XP_006531558.1. XM_006531495.2. [Q6Y5D8-2]
XP_006531561.1. XM_006531498.2. [Q6Y5D8-3]
XP_006531563.1. XM_006531500.1. [Q6Y5D8-4]
UniGeneiMm.165974.

Genome annotation databases

EnsembliENSMUST00000076316; ENSMUSP00000075658; ENSMUSG00000037148. [Q6Y5D8-1]
GeneIDi78514.
KEGGimmu:78514.
UCSCiuc009mhm.1. mouse. [Q6Y5D8-1]
uc009mhn.1. mouse. [Q6Y5D8-3]
uc012gge.1. mouse. [Q6Y5D8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF297029 mRNA. Translation: AAK18174.1.
AF297030 mRNA. Translation: AAK18175.1.
AY179965 mRNA. Translation: AAO62072.1.
AY179966 mRNA. Translation: AAO62073.1.
AY179967 mRNA. Translation: AAO62074.1.
CCDSiCCDS22425.1. [Q6Y5D8-1]
RefSeqiNP_084389.2. NM_030113.2. [Q6Y5D8-1]
XP_006531558.1. XM_006531495.2. [Q6Y5D8-2]
XP_006531561.1. XM_006531498.2. [Q6Y5D8-3]
XP_006531563.1. XM_006531500.1. [Q6Y5D8-4]
UniGeneiMm.165974.

3D structure databases

ProteinModelPortaliQ6Y5D8.
SMRiQ6Y5D8. Positions 14-367, 389-579, 730-786.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219450. 1 interaction.
IntActiQ6Y5D8. 1 interaction.

PTM databases

PhosphoSiteiQ6Y5D8.

Proteomic databases

MaxQBiQ6Y5D8.
PaxDbiQ6Y5D8.
PRIDEiQ6Y5D8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000076316; ENSMUSP00000075658; ENSMUSG00000037148. [Q6Y5D8-1]
GeneIDi78514.
KEGGimmu:78514.
UCSCiuc009mhm.1. mouse. [Q6Y5D8-1]
uc009mhn.1. mouse. [Q6Y5D8-3]
uc012gge.1. mouse. [Q6Y5D8-2]

Organism-specific databases

CTDi79658.
MGIiMGI:1925764. Arhgap10.

Phylogenomic databases

eggNOGiNOG311191.
GeneTreeiENSGT00780000121833.
HOGENOMiHOG000018767.
HOVERGENiHBG067993.
InParanoidiQ6Y5D8.
KOiK13736.
OMAiCFDVEAA.
OrthoDBiEOG71VSS5.
PhylomeDBiQ6Y5D8.
TreeFamiTF316851.

Enzyme and pathway databases

ReactomeiREACT_297947. Rho GTPase cycle.
REACT_322416. Regulation of PAK-2p34 activity by PS-GAP/RHG10.

Miscellaneous databases

ChiTaRSiArhgap10. mouse.
NextBioi349007.
PROiQ6Y5D8.
SOURCEiSearch...

Gene expression databases

BgeeiQ6Y5D8.
CleanExiMM_ARHGAP10.
ExpressionAtlasiQ6Y5D8. baseline and differential.
GenevestigatoriQ6Y5D8.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00620. RhoGAP. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Regulation of CDC42 GTPase by proline-rich tyrosine kinase 2 interacting with PSGAP, a novel pleckstrin homology and Src homology 3 domain containing rhoGAP protein."
    Ren X.-R., Du Q.-S., Huang Y.-Z., Ao S.-Z., Mei L., Xiong W.-C.
    J. Cell Biol. 152:971-984(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 4), FUNCTION, INTERACTION WITH PTK2/FAK1 AND PTK2B/PYK2, SUBCELLULAR LOCATION, PHOSPHORYLATION, MUTAGENESIS OF ARG-418.
    Strain: C57BL/10J.
  2. "Identification and characterization of PS-GAP as a novel regulator of caspase-activated PAK-2."
    Koeppel M.A., McCarthy C.C., Moertl E., Jakobi R.
    J. Biol. Chem. 279:53653-53664(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), FUNCTION, INTERACTION WITH PAK2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: BALB/c.
    Tissue: Heart.

Entry informationi

Entry nameiRHG10_MOUSE
AccessioniPrimary (citable) accession number: Q6Y5D8
Secondary accession number(s): Q6Y5D6
, Q6Y5D7, Q99MT3, Q99MT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 2007
Last sequence update: October 1, 2007
Last modified: March 31, 2015
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.