Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q6Y4S4

- KISS1_MOUSE

UniProt

Q6Y4S4 - KISS1_MOUSE

Protein

Metastasis-suppressor KiSS-1

Gene

Kiss1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Metastasis suppressor protein. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. Intracerebroventricular administration induces an increase in serum LH and FSH levels in prepubertal male and female as well as in adult animals.4 Publications

    GO - Biological processi

    1. generation of ovulation cycle rhythm Source: Ensembl
    2. negative regulation of cell proliferation Source: Ensembl
    3. positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Source: Ensembl
    4. positive regulation of growth hormone secretion Source: Ensembl
    5. positive regulation of luteinizing hormone secretion Source: Ensembl
    6. positive regulation of MAPK cascade Source: Ensembl
    7. positive regulation of synaptic transmission Source: Ensembl

    Enzyme and pathway databases

    ReactomeiREACT_207651. G alpha (q) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Metastasis-suppressor KiSS-1
    Alternative name(s):
    Kisspeptin-1
    Cleaved into the following 2 chains:
    Alternative name(s):
    Kisspeptin-52
    Alternative name(s):
    Metastin45-54
    Gene namesi
    Name:Kiss1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:2663985. Kiss1.

    Subcellular locationi

    GO - Cellular componenti

    1. apical plasma membrane Source: Ensembl
    2. cytoplasm Source: Ensembl
    3. extracellular space Source: Ensembl
    4. neuronal cell body Source: Ensembl
    5. neuron projection Source: Ensembl

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Animals are viable and healthy with no apparent abnormalities but fail to undergo sexual maturation. Mutant female do not progress through the estrous cycle, have thread-like uteri and small ovaries, and do not produce mature Graffian follicles. Mutant males have small testes, and spermatogenesis arrested mainly at the early haploid spermatid stage. Both sexes have low circulating gonadotropin (LH and FSH) and sex steroid (beta-estradiol or testosterone) hormone levels. Migration of GnRH neurons into the hypothalamus appears normal with appropriate axonal connections to the median eminence and total GnRH content. The hypothalamic-pituitary axis is functional, as shown by robust LH secretion after peripheral administration of kisspeptin.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 130111Metastasis-suppressor KiSS-1PRO_0000021552Add
    BLAST
    Peptidei68 – 11952MetastinPRO_0000021553Add
    BLAST
    Peptidei110 – 11910Kisspeptin-10PRO_0000021554

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi71 ↔ 85Sequence Analysis
    Modified residuei110 – 1101PhosphotyrosineBy similarity
    Modified residuei119 – 1191Tyrosine amideBy similarity

    Keywords - PTMi

    Amidation, Cleavage on pair of basic residues, Disulfide bond, Phosphoprotein

    Proteomic databases

    PRIDEiQ6Y4S4.

    PTM databases

    PhosphoSiteiQ6Y4S4.

    Expressioni

    Tissue specificityi

    Weak in all tissue types with highest levels in lung and 15- 17-day embryos. Expressed in areas of the hypothalamus implicated in the neuroendocrine regulation of gonadotropin secretion, including the anteroventral periventricular nucleus, the periventricular nucleus, and the arcuate nucleus.2 Publications

    Gene expression databases

    BgeeiQ6Y4S4.
    GenevestigatoriQ6Y4S4.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni110 – 11910Essential for receptor binding and receptor activation

    Sequence similaritiesi

    Belongs to the KISS1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    GeneTreeiENSGT00390000008827.
    HOVERGENiHBG052261.
    OMAiHYITRIH.
    OrthoDBiEOG79GT8B.
    TreeFamiTF338233.

    Family and domain databases

    InterProiIPR020207. Metastasis-suppressor_KiSS-1.
    [Graphical view]
    PfamiPF15152. Kisspeptin. 1 hit.
    [Graphical view]
    ProDomiPD088121. Metastasis-suppressor_KiSS-1. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6Y4S4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MISMASWQLL LLLCVATYGE PLAKVAPLVK PGSTGQQSGP QELVNAWEKE    50
    SRYAESKPGS AGLRARRSSP CPPVEGPAGR QRPLCASRSR LIPAPRGAVL 100
    VQREKDLSTY NWNSFGLRYG RRQAARAARG 130
    Length:130
    Mass (Da):14,117
    Last modified:March 1, 2005 - v2
    Checksum:iAC9D30CF84B4F41B
    GO
    Isoform 2 (identifier: Q6Y4S4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         25-28: Missing.

    Show »
    Length:126
    Mass (Da):13,737
    Checksum:iA71956212746F37A
    GO

    Sequence cautioni

    The sequence AAO64981.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei25 – 284Missing in isoform 2. 3 PublicationsVSP_012957

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF472576 mRNA. Translation: AAP13356.1.
    AY182231 mRNA. Translation: AAO64981.1. Different initiation.
    AB162440 mRNA. Translation: BAD36756.1.
    AY707858 mRNA. Translation: AAW34131.1.
    BC117046 mRNA. Translation: AAI17047.1.
    BC119348 mRNA. Translation: AAI19349.1.
    CCDSiCCDS48362.1. [Q6Y4S4-2]
    RefSeqiNP_839991.2. NM_178260.3. [Q6Y4S4-2]
    XP_006497355.1. XM_006497292.1.
    XP_006497356.1. XM_006497293.1. [Q6Y4S4-1]
    UniGeneiMm.458301.

    Genome annotation databases

    EnsembliENSMUST00000007433; ENSMUSP00000007433; ENSMUSG00000091251. [Q6Y4S4-2]
    ENSMUST00000178033; ENSMUSP00000136746; ENSMUSG00000091251. [Q6Y4S4-2]
    ENSMUST00000183738; ENSMUSP00000138976; ENSMUSG00000091251. [Q6Y4S4-2]
    ENSMUST00000185157; ENSMUSP00000139313; ENSMUSG00000091251. [Q6Y4S4-2]
    GeneIDi280287.
    KEGGimmu:280287.
    UCSCiuc007cqd.1. mouse. [Q6Y4S4-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF472576 mRNA. Translation: AAP13356.1 .
    AY182231 mRNA. Translation: AAO64981.1 . Different initiation.
    AB162440 mRNA. Translation: BAD36756.1 .
    AY707858 mRNA. Translation: AAW34131.1 .
    BC117046 mRNA. Translation: AAI17047.1 .
    BC119348 mRNA. Translation: AAI19349.1 .
    CCDSi CCDS48362.1. [Q6Y4S4-2 ]
    RefSeqi NP_839991.2. NM_178260.3. [Q6Y4S4-2 ]
    XP_006497355.1. XM_006497292.1.
    XP_006497356.1. XM_006497293.1. [Q6Y4S4-1 ]
    UniGenei Mm.458301.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q6Y4S4.

    Proteomic databases

    PRIDEi Q6Y4S4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000007433 ; ENSMUSP00000007433 ; ENSMUSG00000091251 . [Q6Y4S4-2 ]
    ENSMUST00000178033 ; ENSMUSP00000136746 ; ENSMUSG00000091251 . [Q6Y4S4-2 ]
    ENSMUST00000183738 ; ENSMUSP00000138976 ; ENSMUSG00000091251 . [Q6Y4S4-2 ]
    ENSMUST00000185157 ; ENSMUSP00000139313 ; ENSMUSG00000091251 . [Q6Y4S4-2 ]
    GeneIDi 280287.
    KEGGi mmu:280287.
    UCSCi uc007cqd.1. mouse. [Q6Y4S4-2 ]

    Organism-specific databases

    CTDi 3814.
    MGIi MGI:2663985. Kiss1.

    Phylogenomic databases

    GeneTreei ENSGT00390000008827.
    HOVERGENi HBG052261.
    OMAi HYITRIH.
    OrthoDBi EOG79GT8B.
    TreeFami TF338233.

    Enzyme and pathway databases

    Reactomei REACT_207651. G alpha (q) signalling events.

    Miscellaneous databases

    NextBioi 394046.
    PROi Q6Y4S4.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q6Y4S4.
    Genevestigatori Q6Y4S4.

    Family and domain databases

    InterProi IPR020207. Metastasis-suppressor_KiSS-1.
    [Graphical view ]
    Pfami PF15152. Kisspeptin. 1 hit.
    [Graphical view ]
    ProDomi PD088121. Metastasis-suppressor_KiSS-1. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of mouse metastasis-suppressor KiSS1 and its G-protein-coupled receptor."
      Stafford L.J., Xia C., Ma W., Cai Y., Liu M.
      Cancer Res. 62:5399-5404(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION, TISSUE SPECIFICITY.
      Strain: 129S6/SvEvTac.
      Tissue: Brain.
    2. "Expression of KiSS-1, a metastasis suppressor gene, in trophoblast giant cells of the rat placenta."
      Terao Y., Kumano S., Takatsu Y., Hattori M., Nishimura A., Ohtaki T., Shintani Y.
      Biochim. Biophys. Acta 1678:102-110(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
      Tissue: Embryo.
    3. "Identification and characterization of metastin in the mouse spinal cord."
      Ohsawa M., Chi M., Ohtsubo Y., Brailoiu G.C., Yang J., Chang J., Dun N.J.
      Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Strain: ICR.
      Tissue: Brain.
    4. "The genesis of the human KISS1."
      Xie H., Bonaldo M.F., Soares M.B.
      Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Placenta.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    6. Cited for: TISSUE SPECIFICITY, ROLE IN THE REGULATION OF GONADOTROPIN SECRETION.
    7. "Kisspeptin activation of gonadotropin releasing hormone neurons and regulation of KiSS-1 mRNA in the male rat."
      Irwig M.S., Fraley G.S., Smith J.T., Acohido B.V., Popa S.M., Cunningham M.J., Gottsch M.L., Clifton D.K., Steiner R.A.
      Neuroendocrinology 80:264-272(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INVOLVEMENT IN REGULATION OF GONADOTROPIN SECRETION.
    8. "Advanced vaginal opening and precocious activation of the reproductive axis by KiSS-1 peptide, the endogenous ligand of GPR54."
      Navarro V.M., Fernandez-Fernandez R., Castellano J.M., Roa J., Mayen A., Barreiro M.L., Gaytan F., Aguilar E., Pinilla L., Dieguez C., Tena-Sempere M.
      J. Physiol. (Lond.) 561:379-386(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Characterization of the potent luteinizing hormone-releasing activity of KiSS-1 peptide, the natural ligand of GPR54."
      Navarro V.M., Castellano J.M., Fernandez-Fernandez R., Tovar S., Roa J., Mayen A., Nogueiras R., Vazquez M.J., Barreiro M.L., Magni P., Aguilar E., Dieguez C., Pinilla L., Tena-Sempere M.
      Endocrinology 146:156-163(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: ROLE IN LUTEINIZING HORMONE SECRETION.
    10. Cited for: DIRECT STIMULATION OF GONADOTROPIN-RELEASING HORMONE RELEASE.
    11. "Effects of KiSS-1 peptide, the natural ligand of GPR54, on follicle-stimulating hormone secretion in the rat."
      Navarro V.M., Castellano J.M., Fernandez-Fernandez R., Tovar S., Roa J., Mayen A., Barreiro M.L., Casanueva F.F., Aguilar E., Dieguez C., Pinilla L., Tena-Sempere M.
      Endocrinology 146:1689-1697(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: ROLE IN FOLLICLE-STIMULATING HORMONE SECRETION.
    12. Cited for: DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiKISS1_MOUSE
    AccessioniPrimary (citable) accession number: Q6Y4S4
    Secondary accession number(s): Q149Z8, Q68Y93, Q80XF6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: March 1, 2005
    Last modified: October 1, 2014
    This is version 79 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3