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Q6Y4S4 (KISS1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Metastasis-suppressor KiSS-1
Alternative name(s):
Kisspeptin-1

Cleaved into the following 2 chains:

  1. Metastin
    Alternative name(s):
    Kisspeptin-52
  2. Kisspeptin-10
    Alternative name(s):
    Metastin45-54
Gene names
Name:Kiss1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length130 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Metastasis suppressor protein. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. Intracerebroventricular administration induces an increase in serum LH and FSH levels in prepubertal male and female as well as in adult animals. Ref.6 Ref.8 Ref.9 Ref.11

Subcellular location

Secreted.

Tissue specificity

Weak in all tissue types with highest levels in lung and 15- 17-day embryos. Expressed in areas of the hypothalamus implicated in the neuroendocrine regulation of gonadotropin secretion, including the anteroventral periventricular nucleus, the periventricular nucleus, and the arcuate nucleus. Ref.1 Ref.6

Disruption phenotype

Animals are viable and healthy with no apparent abnormalities but fail to undergo sexual maturation. Mutant female do not progress through the estrous cycle, have thread-like uteri and small ovaries, and do not produce mature Graffian follicles. Mutant males have small testes, and spermatogenesis arrested mainly at the early haploid spermatid stage. Both sexes have low circulating gonadotropin (LH and FSH) and sex steroid (beta-estradiol or testosterone) hormone levels. Migration of GnRH neurons into the hypothalamus appears normal with appropriate axonal connections to the median eminence and total GnRH content. The hypothalamic-pituitary axis is functional, as shown by robust LH secretion after peripheral administration of kisspeptin. Ref.12

Sequence similarities

Belongs to the KISS1 family.

Sequence caution

The sequence AAO64981.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6Y4S4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6Y4S4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     25-28: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 130111Metastasis-suppressor KiSS-1
PRO_0000021552
Peptide68 – 11952Metastin
PRO_0000021553
Peptide110 – 11910Kisspeptin-10
PRO_0000021554

Regions

Region110 – 11910Essential for receptor binding and receptor activation

Amino acid modifications

Modified residue1101Phosphotyrosine By similarity
Modified residue1191Tyrosine amide By similarity
Disulfide bond71 ↔ 85 Potential

Natural variations

Alternative sequence25 – 284Missing in isoform 2.
VSP_012957

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2005. Version 2.
Checksum: AC9D30CF84B4F41B

FASTA13014,117
        10         20         30         40         50         60 
MISMASWQLL LLLCVATYGE PLAKVAPLVK PGSTGQQSGP QELVNAWEKE SRYAESKPGS 

        70         80         90        100        110        120 
AGLRARRSSP CPPVEGPAGR QRPLCASRSR LIPAPRGAVL VQREKDLSTY NWNSFGLRYG 

       130 
RRQAARAARG 

« Hide

Isoform 2 [UniParc].

Checksum: A71956212746F37A
Show »

FASTA12613,737

References

« Hide 'large scale' references
[1]"Identification and characterization of mouse metastasis-suppressor KiSS1 and its G-protein-coupled receptor."
Stafford L.J., Xia C., Ma W., Cai Y., Liu M.
Cancer Res. 62:5399-5404(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION, TISSUE SPECIFICITY.
Strain: 129S6/SvEvTac.
Tissue: Brain.
[2]"Expression of KiSS-1, a metastasis suppressor gene, in trophoblast giant cells of the rat placenta."
Terao Y., Kumano S., Takatsu Y., Hattori M., Nishimura A., Ohtaki T., Shintani Y.
Biochim. Biophys. Acta 1678:102-110(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Tissue: Embryo.
[3]"Identification and characterization of metastin in the mouse spinal cord."
Ohsawa M., Chi M., Ohtsubo Y., Brailoiu G.C., Yang J., Chang J., Dun N.J.
Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: ICR.
Tissue: Brain.
[4]"The genesis of the human KISS1."
Xie H., Bonaldo M.F., Soares M.B.
Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Placenta.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[6]"A role for kisspeptins in the regulation of gonadotropin secretion in the mouse."
Gottsch M.L., Cunningham M.J., Smith J.T., Popa S.M., Acohido B.V., Crowley W.F., Seminara S., Clifton D.K., Steiner R.A.
Endocrinology 145:4073-4077(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, ROLE IN THE REGULATION OF GONADOTROPIN SECRETION.
[7]"Kisspeptin activation of gonadotropin releasing hormone neurons and regulation of KiSS-1 mRNA in the male rat."
Irwig M.S., Fraley G.S., Smith J.T., Acohido B.V., Popa S.M., Cunningham M.J., Gottsch M.L., Clifton D.K., Steiner R.A.
Neuroendocrinology 80:264-272(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INVOLVEMENT IN REGULATION OF GONADOTROPIN SECRETION.
[8]"Advanced vaginal opening and precocious activation of the reproductive axis by KiSS-1 peptide, the endogenous ligand of GPR54."
Navarro V.M., Fernandez-Fernandez R., Castellano J.M., Roa J., Mayen A., Barreiro M.L., Gaytan F., Aguilar E., Pinilla L., Dieguez C., Tena-Sempere M.
J. Physiol. (Lond.) 561:379-386(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Characterization of the potent luteinizing hormone-releasing activity of KiSS-1 peptide, the natural ligand of GPR54."
Navarro V.M., Castellano J.M., Fernandez-Fernandez R., Tovar S., Roa J., Mayen A., Nogueiras R., Vazquez M.J., Barreiro M.L., Magni P., Aguilar E., Dieguez C., Pinilla L., Tena-Sempere M.
Endocrinology 146:156-163(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: ROLE IN LUTEINIZING HORMONE SECRETION.
[10]"Kisspeptin directly stimulates gonadotropin-releasing hormone release via G protein-coupled receptor 54."
Messager S., Chatzidaki E.E., Ma D., Hendrick A.G., Zahn D., Dixon J., Thresher R.R., Malinge I., Lomet D., Carlton M.B., Colledge W.H., Caraty A., Aparicio S.A.J.R.
Proc. Natl. Acad. Sci. U.S.A. 102:1761-1766(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: DIRECT STIMULATION OF GONADOTROPIN-RELEASING HORMONE RELEASE.
[11]"Effects of KiSS-1 peptide, the natural ligand of GPR54, on follicle-stimulating hormone secretion in the rat."
Navarro V.M., Castellano J.M., Fernandez-Fernandez R., Tovar S., Roa J., Mayen A., Barreiro M.L., Casanueva F.F., Aguilar E., Dieguez C., Pinilla L., Tena-Sempere M.
Endocrinology 146:1689-1697(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: ROLE IN FOLLICLE-STIMULATING HORMONE SECRETION.
[12]"Hypogonadotropic hypogonadism in mice lacking a functional Kiss1 gene."
d'Anglemont de Tassigny X., Fagg L.A., Dixon J.P., Day K., Leitch H.G., Hendrick A.G., Zahn D., Franceschini I., Caraty A., Carlton M.B., Aparicio S.A., Colledge W.H.
Proc. Natl. Acad. Sci. U.S.A. 104:10714-10719(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF472576 mRNA. Translation: AAP13356.1.
AY182231 mRNA. Translation: AAO64981.1. Different initiation.
AB162440 mRNA. Translation: BAD36756.1.
AY707858 mRNA. Translation: AAW34131.1.
BC117046 mRNA. Translation: AAI17047.1.
BC119348 mRNA. Translation: AAI19349.1.
CCDSCCDS48362.1. [Q6Y4S4-2]
RefSeqNP_839991.2. NM_178260.3. [Q6Y4S4-2]
XP_006497355.1. XM_006497292.1.
XP_006497356.1. XM_006497293.1. [Q6Y4S4-1]
UniGeneMm.458301.

3D structure databases

ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ6Y4S4.

Proteomic databases

PRIDEQ6Y4S4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000007433; ENSMUSP00000007433; ENSMUSG00000091251. [Q6Y4S4-2]
ENSMUST00000178033; ENSMUSP00000136746; ENSMUSG00000091251. [Q6Y4S4-2]
ENSMUST00000183738; ENSMUSP00000138976; ENSMUSG00000091251. [Q6Y4S4-2]
ENSMUST00000185157; ENSMUSP00000139313; ENSMUSG00000091251. [Q6Y4S4-2]
GeneID280287.
KEGGmmu:280287.
UCSCuc007cqd.1. mouse. [Q6Y4S4-2]

Organism-specific databases

CTD3814.
MGIMGI:2663985. Kiss1.

Phylogenomic databases

GeneTreeENSGT00390000008827.
HOVERGENHBG052261.
OMAHYITRIH.
OrthoDBEOG79GT8B.
TreeFamTF338233.

Gene expression databases

BgeeQ6Y4S4.
GenevestigatorQ6Y4S4.

Family and domain databases

InterProIPR020207. Metastasis-suppressor_KiSS-1.
[Graphical view]
PfamPF15152. Kisspeptin. 1 hit.
[Graphical view]
ProDomPD088121. Metastasis-suppressor_KiSS-1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Other

NextBio394046.
PROQ6Y4S4.
SOURCESearch...

Entry information

Entry nameKISS1_MOUSE
AccessionPrimary (citable) accession number: Q6Y4S4
Secondary accession number(s): Q149Z8, Q68Y93, Q80XF6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: July 9, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot