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Q6Y1H2

- HACD2_HUMAN

UniProt

Q6Y1H2 - HACD2_HUMAN

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Protein

Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2

Gene
PTPLB, HACD2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Responsible for the dehydration step in very long-chain fatty acid (VLCFA) synthesis.1 Publication

Catalytic activityi

A very-long-chain (3R)-3-hydroxyacyl-CoA = a very-long-chain trans-2,3-dehydroacyl-CoA + H2O.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei176 – 1761 By similarity
Active sitei183 – 1831 By similarity

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. protein binding Source: IntAct

GO - Biological processi

  1. fatty acid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 (EC:4.2.1.134)
Alternative name(s):
3-hydroxyacyl-CoA dehydratase 2
Short name:
HACD2
Protein-tyrosine phosphatase-like member B
Gene namesi
Name:PTPLB
Synonyms:HACD2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:9640. PTPLB.

Subcellular locationi

Endoplasmic reticulum membrane; Multi-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 4140Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei42 – 6019Helical; Reviewed predictionAdd
BLAST
Topological domaini61 – 7919Lumenal Reviewed predictionAdd
BLAST
Transmembranei80 – 9718Helical; Reviewed predictionAdd
BLAST
Topological domaini98 – 10710Cytoplasmic Reviewed prediction
Transmembranei108 – 12518Helical; Reviewed predictionAdd
BLAST
Topological domaini126 – 1305Lumenal Reviewed prediction
Transmembranei131 – 14616Helical; Reviewed predictionAdd
BLAST
Topological domaini147 – 16923Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei170 – 18718Helical; Reviewed predictionAdd
BLAST
Topological domaini188 – 21730Lumenal Reviewed predictionAdd
BLAST
Transmembranei218 – 23518Helical; Reviewed predictionAdd
BLAST
Topological domaini236 – 25419Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33983.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 254253Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2PRO_0000349319Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Glycosylationi209 – 2091N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Acetylation, Glycoprotein

Proteomic databases

MaxQBiQ6Y1H2.
PaxDbiQ6Y1H2.
PRIDEiQ6Y1H2.

PTM databases

PhosphoSiteiQ6Y1H2.

Expressioni

Tissue specificityi

Highly expressed in testis, spleen, prostate, colon and heart, followed by moderate expression in thymus, ovary, small intestine, peripheral blood leukocytes, liver, skeletal muscle and pancreas. Weakly detected in kidney, placenta, brain and lung.2 Publications

Gene expression databases

ArrayExpressiQ6Y1H2.
BgeeiQ6Y1H2.
CleanExiHS_PTPLB.
GenevestigatoriQ6Y1H2.

Organism-specific databases

HPAiCAB019282.

Interactioni

Subunit structurei

Interacts with the condensation enzymes of the ELOVL family. Interacts with BCAP31.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BCAP31P515724EBI-530257,EBI-77683

Protein-protein interaction databases

BioGridi128393. 3 interactions.
IntActiQ6Y1H2. 1 interaction.
STRINGi9606.ENSP00000373153.

Structurei

3D structure databases

ProteinModelPortaliQ6Y1H2.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi12 – 187Poly-Gly

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5198.
HOGENOMiHOG000190538.
HOVERGENiHBG057518.
InParanoidiQ6Y1H2.
KOiK10703.
OMAiVREVQSE.
OrthoDBiEOG7X6M18.
PhylomeDBiQ6Y1H2.
TreeFamiTF313326.

Family and domain databases

InterProiIPR007482. Tyr_Pase-like_PTPLA.
[Graphical view]
PANTHERiPTHR11035. PTHR11035. 1 hit.
PfamiPF04387. PTPLA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6Y1H2-1 [UniParc]FASTAAdd to Basket

« Hide

MAAVAATAAA KGNGGGGGRA GAGDASGTRK KKGPGPLATA YLVIYNVVMT    50
AGWLVIAVGL VRAYLAKGSY HSLYYSIEKP LKFFQTGALL EILHCAIGIV 100
PSSVVLTSFQ VMSRVFLIWA VTHSVKEVQS EDSVLLFVIA WTITEIIRYS 150
FYTFSLLNHL PYLIKWARYT LFIVLYPMGV SGELLTIYAA LPFVRQAGLY 200
SISLPNKYNF SFDYYAFLIL IMISYIPIFP QLYFHMIHQR RKILSHTEEH 250
KKFE 254
Length:254
Mass (Da):28,368
Last modified:July 5, 2004 - v1
Checksum:i6B301176C696899E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY191814 mRNA. Translation: AAP20101.1.
AK289983 mRNA. Translation: BAF82672.1.
AC020631 Genomic DNA. No translation available.
AC023165 Genomic DNA. No translation available.
AC025571 Genomic DNA. No translation available.
AC084039 Genomic DNA. No translation available.
BC049369 mRNA. Translation: AAH49369.1.
BC060839 mRNA. Translation: AAH60839.1.
CCDSiCCDS46895.1.
RefSeqiNP_940684.1. NM_198402.3.
UniGeneiHs.705480.

Genome annotation databases

EnsembliENST00000383657; ENSP00000373153; ENSG00000206527.
GeneIDi201562.
KEGGihsa:201562.
UCSCiuc003egj.2. human.

Polymorphism databases

DMDMi74738322.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY191814 mRNA. Translation: AAP20101.1 .
AK289983 mRNA. Translation: BAF82672.1 .
AC020631 Genomic DNA. No translation available.
AC023165 Genomic DNA. No translation available.
AC025571 Genomic DNA. No translation available.
AC084039 Genomic DNA. No translation available.
BC049369 mRNA. Translation: AAH49369.1 .
BC060839 mRNA. Translation: AAH60839.1 .
CCDSi CCDS46895.1.
RefSeqi NP_940684.1. NM_198402.3.
UniGenei Hs.705480.

3D structure databases

ProteinModelPortali Q6Y1H2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 128393. 3 interactions.
IntActi Q6Y1H2. 1 interaction.
STRINGi 9606.ENSP00000373153.

PTM databases

PhosphoSitei Q6Y1H2.

Polymorphism databases

DMDMi 74738322.

Proteomic databases

MaxQBi Q6Y1H2.
PaxDbi Q6Y1H2.
PRIDEi Q6Y1H2.

Protocols and materials databases

DNASUi 201562.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000383657 ; ENSP00000373153 ; ENSG00000206527 .
GeneIDi 201562.
KEGGi hsa:201562.
UCSCi uc003egj.2. human.

Organism-specific databases

CTDi 201562.
GeneCardsi GC03M123209.
HGNCi HGNC:9640. PTPLB.
HPAi CAB019282.
neXtProti NX_Q6Y1H2.
PharmGKBi PA33983.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5198.
HOGENOMi HOG000190538.
HOVERGENi HBG057518.
InParanoidi Q6Y1H2.
KOi K10703.
OMAi VREVQSE.
OrthoDBi EOG7X6M18.
PhylomeDBi Q6Y1H2.
TreeFami TF313326.

Miscellaneous databases

GenomeRNAii 201562.
NextBioi 90164.
PROi Q6Y1H2.

Gene expression databases

ArrayExpressi Q6Y1H2.
Bgeei Q6Y1H2.
CleanExi HS_PTPLB.
Genevestigatori Q6Y1H2.

Family and domain databases

InterProi IPR007482. Tyr_Pase-like_PTPLA.
[Graphical view ]
PANTHERi PTHR11035. PTHR11035. 1 hit.
Pfami PF04387. PTPLA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast split-ubiquitin membrane protein two-hybrid screen identifies BAP31 as a regulator of the turnover of endoplasmic reticulum-associated protein tyrosine phosphatase-like B."
    Wang B., Pelletier J., Massaad M.J., Herscovics A., Shore G.C.
    Mol. Cell. Biol. 24:2767-2778(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH BCAP31.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "The DNA sequence, annotation and analysis of human chromosome 3."
    Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
    , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
    Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain and Testis.
  5. "Characterization of four mammalian 3-hydroxyacyl-CoA dehydratases involved in very long-chain fatty acid synthesis."
    Ikeda M., Kanao Y., Yamanaka M., Sakuraba H., Mizutani Y., Igarashi Y., Kihara A.
    FEBS Lett. 582:2435-2440(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, INTERACTION WITH ELOVL FAMILY.
  6. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHACD2_HUMAN
AccessioniPrimary (citable) accession number: Q6Y1H2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Turns over rapidly through degradation by the proteasome system.

Caution

Shares some similarity with tyrosine phosphatase proteins but it has probably no phosphatase activity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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