Reviewed, UniProtKB/Swiss-Prot Q6Y0Z3 (XYL1_CANPA)

Names and origin

Protein names

Recommended name:
    NADH-dependent D-xylose reductase
      Short name=XR
    EC=1.1.1.175

Gene names

Name:

XYL1

Organism

Candida parapsilosis (Yeast)

Taxonomic identifier

5480 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reduces D-xylose into xylitol. Preferentially utilizes NADH as a cosubstrate.
Catalytic activity	D-xylose + NAD+ = D-xylonolactone + NADH.
Pathway	Carbohydrate metabolism; D-xylose degradation.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Xylose metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	D-xylose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-xylose 1-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	NADH-dependent D-xylose reductase		PRO_0000124659
Regions
	Nucleotide binding	220 – 286	67	NAD By similarity
Sites
	Active site	54	1	Proton donor By similarity
	Binding site	116	1	Substrate By similarity
	Site	83	1	Lowers pKa of active site Tyr By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6Y0Z3-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C64951D131707E19	FASTA	324	36,629
	10 20 30 40 50 60 MSTATASPAV KLNSGYEIPL VGFGCWKLTN DVASDQIYRA IKSGYRLFDG AEDYANEQEV 70 80 90 100 110 120 GEGIKRAIKE GIVKREELFI TSKLWNSFHD KKNVEVALMK TLSDLNLDYV DLFYIHFPIA 130 140 150 160 170 180 QKPVPIEKKY PPGFYCGDGD KWSIEEVPLL DTWRALEKLV DQGLAKSIGI SNFSAQLIYD 190 200 210 220 230 240 LIRGCTIKPV ALQIEHHPYL TQPKLVEYVQ LHDIQITGYS SFGPQSFLEM DLKRALDTPV 250 260 270 280 290 300 LLEEPTVKSI ADKHGKSPAQ VLLRYQTQRG IAVIPRSNSP DRMAQNLSVI DFELTQDDLQ 310 320 AIAELDCNLR FNEPWDFSNI PVFV

References

[1]

"Cloning and characterization of the xyl1 gene, encoding an NADH-preferring xylose reductase from Candida parapsilosis, and its functional expression in Candida tropicalis." Lee J.K., Koo B.S., Kim S.Y. Appl. Environ. Microbiol. 69:6179-6188(2003) [PubMed: 14532079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION. Strain: KFCC-10875.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY193716 mRNA. Translation: AAO91803.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.175. 3487.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYL1_CANPA

Accession

Primary (citable) accession number: Q6Y0Z3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)