Q6XZF7 (DNMBP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dynamin-binding protein

Alternative name(s):
Scaffold protein Tuba

Gene names

Name:	DNMBP
Synonyms:	KIAA1010

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1577 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Scaffold protein that links dynamin with actin-regulating proteins. May play a role in membrane trafficking between the cell surface and the Golgi By similarity.
Subunit structure	Binds DNM1 via its N-terminal SH3 domains. The C-terminal SH3 domain binds a complex containing actin, tubulin, Hsp70 and actin-regulatory proteins, such as ENAH, EVL, WASL, WIRE, CR16, WAVE1 and NAP1L1 By similarity. Interacts with FASLG. Ref.5
Subcellular location	Cytoplasm By similarity. Golgi apparatus › Golgi stack By similarity. Cytoplasm › cytoskeleton By similarity. Cell junction › synapse By similarity. Note: Localized to synapses and Golgi stacks By similarity.
Tissue specificity	Detected in heart, brain, lung, liver, skeletal muscle, kidney and pancreas. Ref.1
Sequence similarities	Contains 1 BAR domain. Contains 1 DH (DBL-homology) domain. Contains 6 SH3 domains.

Ontologies

Keywords
Cellular component	Cell junction Cytoplasm Cytoskeleton Golgi apparatus Synapse
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Repeat SH3 domain
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro regulation of Rho protein signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi stack Inferred from electronic annotation. Source: UniProtKB-SubCell cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell synapse Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	Rho guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BIN3	Q9NQY0	2	EBI-2483419,EBI-2653038

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6XZF7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6XZF7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-754: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1577

1577

Dynamin-binding protein

PRO_0000079959

Regions

Domain

2 – 61

60

SH3 1

Domain

66 – 126

61

SH3 2

Domain

145 – 204

60

SH3 3

Domain

243 – 302

60

SH3 4

Domain

784 – 967

184

DH

Domain

1008 – 1217

210

BAR

Domain

1285 – 1348

64

SH3 5

Domain

1513 – 1576

64

SH3 6

Coiled coil

693 – 757

65

Potential

Coiled coil

1136 – 1173

38

Potential

Compositional bias

610 – 649

40

Pro-rich

Compositional bias

1351 – 1449

99

Ser-rich

Natural variations

Alternative sequence

1 – 754

754

Missing in isoform 2.

VSP_012079

Natural variant

81

1

E → D.
Corresponds to variant rs12267912 [ dbSNP | Ensembl ].

VAR_050955

Natural variant

373

1

N → K.
Corresponds to variant rs35924554 [ dbSNP | Ensembl ].

VAR_050956

Natural variant

914

1

N → K.
Corresponds to variant rs7919507 [ dbSNP | Ensembl ].

VAR_050957

Natural variant

1413

1

C → W. Ref.3
Corresponds to variant rs11190305 [ dbSNP | Ensembl ].

VAR_024339

Experimental info

Sequence conflict

831

1

M → T in AAH41628. Ref.3

Secondary structure

1

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1577

Helix Strand Turn

Details...

Beta strand

247 – 252

6

Beta strand

269 – 275

7

Beta strand

280 – 285

6

Beta strand

288 – 293

6

Helix

294 – 296

3

Beta strand

297 – 300

4

Helix

1278 – 1283

6

Helix

1286 – 1288

3

Beta strand

1289 – 1294

6

Beta strand

1300 – 1302

3

Beta strand

1311 – 1317

7

Beta strand

1324 – 1330

7

Beta strand

1332 – 1339

8

Helix

1340 – 1342

3

Beta strand

1343 – 1345

3

Beta strand

1518 – 1522

5

Beta strand

1528 – 1531

4

Beta strand

1539 – 1545

7

Beta strand

1554 – 1561

8

Beta strand

1563 – 1567

5

Helix

1568 – 1570

3

Beta strand

1571 – 1573

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 5A1FDCB06124F627	FASTA	1,577	177,347
	10 20 30 40 50 60 MEAGSVVRAI FDFCPSVSEE LPLFVGDIIE VLAVVDEFWL LGKKEDVTGQ FPSSFVEIVT 70 80 90 100 110 120 IPSLKEGERL FVCICEFTSQ ELDNLPLHRG DLVILDGIPT AGWLQGRSCW GARGFFPSSC 130 140 150 160 170 180 VRELCLSSQS RQWHSQSALF QIPEYSMGQA RALMGLSAQL DEELDFREGD VITIIGVPEP 190 200 210 220 230 240 GWFEGELEGR RGIFPEGFVE LLGPLRTVDE SVSSGNQDDC IVNGEVDTPV GEEEIGPDED 250 260 270 280 290 300 EEEPGTYGVA LYRFQALEPN ELDFEVGDKI RILATLEDGW LEGSLKGRTG IFPYRFVKLC 310 320 330 340 350 360 PDTRVEETMA LPQEGSLARI PETSLDCLEN TLGVEEQRHE TSDHEAEEPD CIISEAPTSP 370 380 390 400 410 420 LGHLTSEYDT DRNSYQDEDT AGGPPRSPGV EWEMPLATDS PTSDPTEVVN GISSQPQVPF 430 440 450 460 470 480 HPNLQKSQYY STVGGSHPHS EQYPDLLPLE ARTRDYASLP PKRMYSQLKT LQKPVLPLYR 490 500 510 520 530 540 GSSVSASRVV KPRQSSPQLH NLASYTKKHH TSSVYSISER LEMKPGPQAQ GLVMEAATHS 550 560 570 580 590 600 QGDGSTDLDS KLTQQLIEFE KSLAGPGTEP DKILRHFSIM DFNSEKDIVR GSSKLITEQE 610 620 630 640 650 660 LPERRKALRP PPPRPCTPVS TSPHLLVDQN LKPAPPLVVR PSRPAPLPPS AQQRTNAVSP 670 680 690 700 710 720 KLLSRHRPTC ETLEKEGPGH MGRSLDQTSP CPLVLVRIEE MERDLDMYSR AQEELNLMLE 730 740 750 760 770 780 EKQDESSRAE TLEDLKFCES NIESLNMELQ QLREMTLLSS QSSSLVAPSG SVSAENPEQR 790 800 810 820 830 840 MLEKRAKVIE ELLQTERDYI RDLEMCIERI MVPMQQAQVP NIDFEGLFGN MQMVIKVSKQ 850 860 870 880 890 900 LLAALEISDA VGPVFLGHRD ELEGTYKIYC QNHDEAIALL EIYEKDEKIQ KHLQDSLADL 910 920 930 940 950 960 KSLYNEWGCT NYINLGSFLI KPVQRVMRYP LLLMELLNST PESHPDKVPL TNAVLAVKEI 970 980 990 1000 1010 1020 NVNINEYKRR KDLVLKYRKG DEDSLMEKIS KLNIHSIIKK SNRVSSHLKH LTGFAPQIKD 1030 1040 1050 1060 1070 1080 EVFEETEKNF RMQERLIKSF IRDLSLYLQH IRESACVKVV AAVSMWDVCM ERGHRDLEQF 1090 1100 1110 1120 1130 1140 ERVHRYISDQ LFTNFKERTE RLVISPLNQL LSMFTGPHKL VQKRFDKLLD FYNCTERAEK 1150 1160 1170 1180 1190 1200 LKDKKTLEEL QSARNNYEAL NAQLLDELPK FHQYAQGLFT NCVHGYAEAH CDFVHQALEQ 1210 1220 1230 1240 1250 1260 LKPLLSLLKV AGREGNLIAI FHEEHSRVLQ QLQVFTFFPE SLPATKKPFE RKTIDRQSAR 1270 1280 1290 1300 1310 1320 KPLLGLPSYM LQSEELRASL LARYPPEKLF QAERNFNAAQ DLDVSLLEGD LVGVIKKKDP 1330 1340 1350 1360 1370 1380 MGSQNRWLID NGVTKGFVYS SFLKPYNPRR SHSDASVGSH SSTESEHGSS SPRFPRQNSG 1390 1400 1410 1420 1430 1440 STLTFNPSSM AVSFTSGSCQ KQPQDASPPP KECDQGTLSA SLNPSNSESS PSRCPSDPDS 1450 1460 1470 1480 1490 1500 TSQPRSGDSA DVARDVKQPT ATPRSYRNFR HPEIVGYSVP GRNGQSQDLV KGCARTAQAP 1510 1520 1530 1540 1550 1560 EDRSTEPDGS EAEGNQVYFA VYTFKARNPN ELSVSANQKL KILEFKDVTG NTEWWLAEVN 1570 GKKGYVPSNY IRKTEYT
	Isoform 2 [UniParc]. Checksum: 535FFA230587539F	FASTA	823	93,498
	10 20 30 40 50 60 MTLLSSQSSS LVAPSGSVSA ENPEQRMLEK RAKVIEELLQ TERDYIRDLE MCIERIMVPM 70 80 90 100 110 120 QQAQVPNIDF EGLFGNMQMV IKVSKQLLAA LEISDAVGPV FLGHRDELEG TYKIYCQNHD 130 140 150 160 170 180 EAIALLEIYE KDEKIQKHLQ DSLADLKSLY NEWGCTNYIN LGSFLIKPVQ RVMRYPLLLM 190 200 210 220 230 240 ELLNSTPESH PDKVPLTNAV LAVKEINVNI NEYKRRKDLV LKYRKGDEDS LMEKISKLNI 250 260 270 280 290 300 HSIIKKSNRV SSHLKHLTGF APQIKDEVFE ETEKNFRMQE RLIKSFIRDL SLYLQHIRES 310 320 330 340 350 360 ACVKVVAAVS MWDVCMERGH RDLEQFERVH RYISDQLFTN FKERTERLVI SPLNQLLSMF 370 380 390 400 410 420 TGPHKLVQKR FDKLLDFYNC TERAEKLKDK KTLEELQSAR NNYEALNAQL LDELPKFHQY 430 440 450 460 470 480 AQGLFTNCVH GYAEAHCDFV HQALEQLKPL LSLLKVAGRE GNLIAIFHEE HSRVLQQLQV 490 500 510 520 530 540 FTFFPESLPA TKKPFERKTI DRQSARKPLL GLPSYMLQSE ELRASLLARY PPEKLFQAER 550 560 570 580 590 600 NFNAAQDLDV SLLEGDLVGV IKKKDPMGSQ NRWLIDNGVT KGFVYSSFLK PYNPRRSHSD 610 620 630 640 650 660 ASVGSHSSTE SEHGSSSPRF PRQNSGSTLT FNPSSMAVSF TSGSCQKQPQ DASPPPKECD 670 680 690 700 710 720 QGTLSASLNP SNSESSPSRC PSDPDSTSQP RSGDSADVAR DVKQPTATPR SYRNFRHPEI 730 740 750 760 770 780 VGYSVPGRNG QSQDLVKGCA RTAQAPEDRS TEPDGSEAEG NQVYFAVYTF KARNPNELSV 790 800 810 820 SANQKLKILE FKDVTGNTEW WLAEVNGKKG YVPSNYIRKT EYT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tuba, a novel protein containing bin/amphiphysin/Rvs and Dbl homology domains, links dynamin to regulation of the actin cytoskeleton." Salazar M.A., Kwiatkowski A.V., Pellegrini L., Cestra G., Butler M.H., Rossman K.L., Serna D.M., Sondek J., Gertler F.B., De Camilli P. J. Biol. Chem. 278:49031-49043(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING, TISSUE SPECIFICITY. Tissue: Brain and Skeletal muscle.
[2]	"Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 6:63-70(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 263-1577 (ISOFORM 1). Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT TRP-1413. Tissue: Uterus.
[4]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[5]	"Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening." Voss M., Lettau M., Janssen O. BMC Immunol. 10:53-53(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FASLG.
[6]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"NMR structure of SH3 domain of hypothetical protein BAA76854.1." RIKEN structural genomics initiative (RSGI) Submitted (DEC-2003) to the PDB data bank Cited for: STRUCTURE BY NMR OF 1278-1353 AND 1510-1575.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY196211 mRNA. Translation: AAP34307.1.
AB023227 mRNA. Translation: BAA76854.1.
BC041628 mRNA. Translation: AAH41628.1.

IPI

IPI00174025.
IPI00480148.

RefSeq

NP_056036.1. NM_015221.2.

UniGene

Hs.500771.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1UG1	NMR	-	A	1278-1356	[»]
1UHC	NMR	-	A	1509-1575	[»]
4GLM	X-ray	1.90	A/B/C/D	246-301	[»]

ProteinModelPortal

Q6XZF7.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q6XZF7. 4 interactions.

STRING

9606.ENSP00000315659.

PTM databases

PhosphoSite

Q6XZF7.

Polymorphism databases

DMDM

56404535.

Proteomic databases

PaxDb

Q6XZF7.

PRIDE

Q6XZF7.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000324109; ENSP00000315659; ENSG00000107554.
ENST00000543621; ENSP00000443657; ENSG00000107554.

GeneID

23268.

KEGG

hsa:23268.

UCSC

uc001kqg.2. human.

Organism-specific databases

CTD

23268.

GeneCards

GC10M101625.

HGNC

HGNC:30373. DNMBP.

HPA

HPA037878.

MIM

611282. gene.

neXtProt

NX_Q6XZF7.

PharmGKB

PA134950706.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5422.

HOGENOM

HOG000112243.

HOVERGEN

HBG051383.

PhylomeDB

Q6XZF7.

Gene expression databases

ArrayExpress

Q6XZF7.

Bgee

Q6XZF7.

CleanEx

HS_DNMBP.

Genevestigator

Q6XZF7.

GermOnline

ENSG00000107554. Homo sapiens.

Family and domain databases

Gene3D

1.20.1270.60. 1 hit.
1.20.900.10. 1 hit.

InterPro

IPR027267. AH/BAR-dom.
IPR004148. BAR_dom.
IPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR000108. p67phox.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]

Pfam

PF03114. BAR. 1 hit.
PF00621. RhoGEF. 1 hit.
PF00018. SH3_1. 4 hits.
PF07653. SH3_2. 1 hit.
[Graphical view]

PRINTS

PR00499. P67PHOX.

SMART

SM00721. BAR. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 6 hits.
[Graphical view]

SUPFAM

SSF48065. DH-domain. 1 hit.
SSF50044. SH3. 6 hits.

PROSITE

PS51021. BAR. 1 hit.
PS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50002. SH3. 5 hits.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

DNMBP. human.

EvolutionaryTrace

Q6XZF7.

GenomeRNAi

23268.

NextBio

45022.

SOURCE

Search...

Entry information

Entry name

DNMBP_HUMAN

Accession

Primary (citable) accession number: Q6XZF7
Secondary accession number(s): Q8IVY3, Q9Y2L3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.