Q6XVN8 (MLP3A_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Microtubule-associated proteins 1A/1B light chain 3A

Alternative name(s):
Autophagy-related protein LC3 A
Autophagy-related ubiquitin-like modifier LC3 A
MAP1 light chain 3-like protein 1
MAP1A/MAP1B light chain 3 A
Short name=MAP1A/MAP1B LC3 A
Microtubule-associated protein 1 light chain 3 alpha

Gene names

Name:

Map1lc3a

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	121 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in formation of autophagosomal vacuoles (autophagosomes) By similarity.
Subunit structure	3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins By similarity. Interacts with TP53INP2 By similarity. Interacts with TP53INP1 and SQSTM1. Directly interacts with SQSTM1; this interaction leads to MAP1LC3A recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy By similarity. Ref.4
Subcellular location	Cytoplasm › cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle › autophagosome membrane; Lipid-anchor By similarity. Cytoplasmic vesicle › autophagosome By similarity. Note: LC3-II binds to the autophagic membranes By similarity.
Post-translational modification	The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II By similarity.
Sequence similarities	Belongs to the MAP1 LC3 family.

Ontologies

Keywords
Biological process	Autophagy Ubl conjugation pathway
Cellular component	Cytoplasm Cytoplasmic vesicle Cytoskeleton Membrane Microtubule
PTM	Lipoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	autophagic vacuole assembly Inferred from direct assay PubMed 11060023. Source: UniProtKB
Cellular_component	autophagic vacuole membrane Inferred from direct assay PubMed 16300744PubMed 16420522PubMed 19337031. Source: RGD cytoplasmic vesicle Inferred from electronic annotation. Source: UniProtKB-KW cytosol Inferred from direct assay PubMed 19337031. Source: RGD endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell late endosome Inferred from direct assay PubMed 19337031. Source: RGD microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	phosphatidylethanolamine binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 120	120	Microtubule-associated proteins 1A/1B light chain 3A		PRO_0000017196
	Propeptide	121	1	Removed in mature form Probable		PRO_0000017197
Amino acid modifications
	Lipidation	120	1	Phosphatidylethanolamine amidated glycine Probable

Sequences

	Sequence		Length	Mass (Da)
	Q6XVN8 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 48C1FBE8F7892AF3	FASTA	121	14,272
	10 20 30 40 50 60 MPSDRPFKQR RSFADRCKEV QQIRDQHPSK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM 70 80 90 100 110 120 SELVKIIRRR LQLNPTQAFF LLVNQHSMVS VSTPIADIYE QEKDEDGFLY MVYASQETFG F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of rat lc3 orthologs." Dang Y., Yu L., Wu J., Pei Y. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[3]	Lubec G., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 31-37, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain.
[4]	"TP53INP1, a tumor suppressor, interacts with LC3 and ATG8-family proteins through the LC3-interacting region (LIR) and promotes autophagy-dependent cell death." Seillier M., Peuget S., Gayet O., Gauthier C., N'guessan P., Monte M., Carrier A., Iovanna J.L., Dusetti N.J. Cell Death Differ. 19:1525-1535(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TP53INP1 AND SQSTM1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY206668 mRNA. Translation: AAP42560.1. BC086389 mRNA. Translation: AAH86389.1.
IPI	IPI00357953.
RefSeq	NP_955794.1. NM_199500.2.
UniGene	Rn.3135.
3D structure databases
ProteinModelPortal	Q6XVN8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6XVN8. 2 interactions.
STRING	10116.ENSRNOP00000032429.
PTM databases
PhosphoSite	Q6XVN8.
Proteomic databases
PaxDb	Q6XVN8.
PRIDE	Q6XVN8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000035060; ENSRNOP00000032429; ENSRNOG00000025443.
GeneID	362245.
KEGG	rno:362245.
UCSC	RGD:735183. rat.
Organism-specific databases
CTD	84557.
RGD	735183. Map1lc3a.
Phylogenomic databases
eggNOG	NOG264390.
GeneTree	ENSGT00390000012937.
HOGENOM	HOG000232034.
HOVERGEN	HBG051706.
InParanoid	Q6XVN8.
KO	K10435.
OMA	TPISEIY.
OrthoDB	EOG4PC9TJ.
Gene expression databases
Genevestigator	Q6XVN8.
GermOnline	ENSRNOG00000025443. Rattus norvegicus.
Family and domain databases
InterPro	IPR004241. Atg8_ubiquitin-like. [Graphical view]
PANTHER	PTHR10969. PTHR10969. 1 hit.
Pfam	PF02991. Atg8. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	679168.

Entry information

Entry name

MLP3A_RAT

Accession

Primary (citable) accession number: Q6XVN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program