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Q6XVN8

- MLP3A_RAT

UniProt

Q6XVN8 - MLP3A_RAT

Protein

Microtubule-associated proteins 1A/1B light chain 3A

Gene

Map1lc3a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei120 – 1212Cleavage; by ATG4BBy similarity

    GO - Molecular functioni

    1. phosphatidylethanolamine binding Source: Ensembl
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. autophagic vacuole assembly Source: UniProtKB
    2. autophagy Source: RGD
    3. mitochondrion degradation Source: Ensembl

    Keywords - Biological processi

    Autophagy, Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Microtubule-associated proteins 1A/1B light chain 3A
    Alternative name(s):
    Autophagy-related protein LC3 A
    Autophagy-related ubiquitin-like modifier LC3 A
    MAP1 light chain 3-like protein 1
    MAP1A/MAP1B light chain 3 A
    Short name:
    MAP1A/MAP1B LC3 A
    Microtubule-associated protein 1 light chain 3 alpha
    Gene namesi
    Name:Map1lc3a
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 3

    Organism-specific databases

    RGDi735183. Map1lc3a.

    Subcellular locationi

    Cytoplasmcytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicleautophagosome membrane By similarity; Lipid-anchor By similarity
    Note: LC3-II binds to the autophagic membranes.By similarity

    GO - Cellular componenti

    1. autophagic vacuole Source: UniProtKB
    2. autophagic vacuole membrane Source: RGD
    3. cytoplasm Source: UniProtKB
    4. cytoplasmic vesicle Source: UniProtKB-KW
    5. cytosol Source: RGD
    6. late endosome Source: RGD
    7. microtubule Source: UniProtKB-KW
    8. organelle membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 120120Microtubule-associated proteins 1A/1B light chain 3APRO_0000017196Add
    BLAST
    Propeptidei121 – 1211Removed in mature formCuratedPRO_0000017197

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei12 – 121Phosphoserine; by PKABy similarity
    Lipidationi120 – 1201Phosphatidylethanolamine amidated glycineCurated

    Post-translational modificationi

    The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.1 Publication
    Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity By similarity.By similarity

    Keywords - PTMi

    Lipoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ6XVN8.
    PRIDEiQ6XVN8.

    PTM databases

    PhosphoSiteiQ6XVN8.

    Expressioni

    Gene expression databases

    GenevestigatoriQ6XVN8.

    Interactioni

    Subunit structurei

    3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins By similarity. Interacts with TP53INP2 By similarity. Interacts with TP53INP1 and SQSTM1. Directly interacts with SQSTM1; this interaction leads to MAP1LC3A recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy By similarity. Interacts with ATG13 and ULK1 By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ6XVN8. 5 interactions.
    STRINGi10116.ENSRNOP00000032429.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6XVN8.
    SMRiQ6XVN8. Positions 2-121.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ATG8 family.Curated

    Phylogenomic databases

    eggNOGiNOG264390.
    GeneTreeiENSGT00390000012937.
    HOGENOMiHOG000232034.
    HOVERGENiHBG051706.
    InParanoidiQ6XVN8.
    KOiK10435.
    OMAiRTFADRC.
    OrthoDBiEOG7XH6RV.
    PhylomeDBiQ6XVN8.
    TreeFamiTF312964.

    Family and domain databases

    InterProiIPR004241. Atg8_like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PANTHERiPTHR10969. PTHR10969. 1 hit.
    PfamiPF02991. Atg8. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q6XVN8-1 [UniParc]FASTAAdd to Basket

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    MPSDRPFKQR RSFADRCKEV QQIRDQHPSK IPVIIERYKG EKQLPVLDKT    50
    KFLVPDHVNM SELVKIIRRR LQLNPTQAFF LLVNQHSMVS VSTPIADIYE 100
    QEKDEDGFLY MVYASQETFG F 121
    Length:121
    Mass (Da):14,272
    Last modified:July 5, 2004 - v1
    Checksum:i48C1FBE8F7892AF3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY206668 mRNA. Translation: AAP42560.1.
    BC086389 mRNA. Translation: AAH86389.1.
    RefSeqiNP_955794.1. NM_199500.2.
    UniGeneiRn.3135.

    Genome annotation databases

    EnsembliENSRNOT00000035060; ENSRNOP00000032429; ENSRNOG00000025443.
    GeneIDi362245.
    KEGGirno:362245.
    UCSCiRGD:735183. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY206668 mRNA. Translation: AAP42560.1 .
    BC086389 mRNA. Translation: AAH86389.1 .
    RefSeqi NP_955794.1. NM_199500.2.
    UniGenei Rn.3135.

    3D structure databases

    ProteinModelPortali Q6XVN8.
    SMRi Q6XVN8. Positions 2-121.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q6XVN8. 5 interactions.
    STRINGi 10116.ENSRNOP00000032429.

    PTM databases

    PhosphoSitei Q6XVN8.

    Proteomic databases

    PaxDbi Q6XVN8.
    PRIDEi Q6XVN8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000035060 ; ENSRNOP00000032429 ; ENSRNOG00000025443 .
    GeneIDi 362245.
    KEGGi rno:362245.
    UCSCi RGD:735183. rat.

    Organism-specific databases

    CTDi 84557.
    RGDi 735183. Map1lc3a.

    Phylogenomic databases

    eggNOGi NOG264390.
    GeneTreei ENSGT00390000012937.
    HOGENOMi HOG000232034.
    HOVERGENi HBG051706.
    InParanoidi Q6XVN8.
    KOi K10435.
    OMAi RTFADRC.
    OrthoDBi EOG7XH6RV.
    PhylomeDBi Q6XVN8.
    TreeFami TF312964.

    Miscellaneous databases

    NextBioi 679168.
    PROi Q6XVN8.

    Gene expression databases

    Genevestigatori Q6XVN8.

    Family and domain databases

    InterProi IPR004241. Atg8_like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    PANTHERi PTHR10969. PTHR10969. 1 hit.
    Pfami PF02991. Atg8. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of rat lc3 orthologs."
      Dang Y., Yu L., Wu J., Pei Y.
      Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Ovary.
    3. Lubec G., Kang S.U.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 31-37, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain.
    4. "Effects of RNA interference of Atg4B on the limited proteolysis of LC3 in PC12 cells and expression of Atg4B in various rat tissues."
      Yoshimura K., Shibata M., Koike M., Gotoh K., Fukaya M., Watanabe M., Uchiyama Y.
      Autophagy 2:200-208(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE BY ATG4B.
    5. "TP53INP1, a tumor suppressor, interacts with LC3 and ATG8-family proteins through the LC3-interacting region (LIR) and promotes autophagy-dependent cell death."
      Seillier M., Peuget S., Gayet O., Gauthier C., N'guessan P., Monte M., Carrier A., Iovanna J.L., Dusetti N.J.
      Cell Death Differ. 19:1525-1535(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TP53INP1 AND SQSTM1.

    Entry informationi

    Entry nameiMLP3A_RAT
    AccessioniPrimary (citable) accession number: Q6XVN8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3