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Q6XVN8 (MLP3A_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Microtubule-associated proteins 1A/1B light chain 3A
Alternative name(s):
Autophagy-related protein LC3 A
Autophagy-related ubiquitin-like modifier LC3 A
MAP1 light chain 3-like protein 1
MAP1A/MAP1B light chain 3 A
Short name=MAP1A/MAP1B LC3 A
Microtubule-associated protein 1 light chain 3 alpha
Gene names
Name:Map1lc3a
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length121 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation By similarity.

Subunit structure

3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins By similarity. Interacts with TP53INP2 By similarity. Interacts with TP53INP1 and SQSTM1. Directly interacts with SQSTM1; this interaction leads to MAP1LC3A recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy By similarity. Interacts with ATG13 and ULK1 By similarity. Ref.5

Subcellular location

Cytoplasmcytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicleautophagosome membrane; Lipid-anchor By similarity. Note: LC3-II binds to the autophagic membranes By similarity.

Post-translational modification

The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Ref.4

Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity By similarity.

Sequence similarities

Belongs to the ATG8 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 120120Microtubule-associated proteins 1A/1B light chain 3A
PRO_0000017196
Propeptide1211Removed in mature form Probable
PRO_0000017197

Sites

Site120 – 1212Cleavage; by ATG4B By similarity

Amino acid modifications

Modified residue121Phosphoserine; by PKA By similarity
Lipidation1201Phosphatidylethanolamine amidated glycine Probable

Sequences

Sequence LengthMass (Da)Tools
Q6XVN8 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 48C1FBE8F7892AF3

FASTA12114,272
        10         20         30         40         50         60 
MPSDRPFKQR RSFADRCKEV QQIRDQHPSK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM 

        70         80         90        100        110        120 
SELVKIIRRR LQLNPTQAFF LLVNQHSMVS VSTPIADIYE QEKDEDGFLY MVYASQETFG 


F 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of rat lc3 orthologs."
Dang Y., Yu L., Wu J., Pei Y.
Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary.
[3]Lubec G., Kang S.U.
Submitted (JUL-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 31-37, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain.
[4]"Effects of RNA interference of Atg4B on the limited proteolysis of LC3 in PC12 cells and expression of Atg4B in various rat tissues."
Yoshimura K., Shibata M., Koike M., Gotoh K., Fukaya M., Watanabe M., Uchiyama Y.
Autophagy 2:200-208(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: CLEAVAGE BY ATG4B.
[5]"TP53INP1, a tumor suppressor, interacts with LC3 and ATG8-family proteins through the LC3-interacting region (LIR) and promotes autophagy-dependent cell death."
Seillier M., Peuget S., Gayet O., Gauthier C., N'guessan P., Monte M., Carrier A., Iovanna J.L., Dusetti N.J.
Cell Death Differ. 19:1525-1535(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TP53INP1 AND SQSTM1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY206668 mRNA. Translation: AAP42560.1.
BC086389 mRNA. Translation: AAH86389.1.
RefSeqNP_955794.1. NM_199500.2.
UniGeneRn.3135.

3D structure databases

ProteinModelPortalQ6XVN8.
SMRQ6XVN8. Positions 2-121.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ6XVN8. 5 interactions.
STRING10116.ENSRNOP00000032429.

PTM databases

PhosphoSiteQ6XVN8.

Proteomic databases

PaxDbQ6XVN8.
PRIDEQ6XVN8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000035060; ENSRNOP00000032429; ENSRNOG00000025443.
GeneID362245.
KEGGrno:362245.
UCSCRGD:735183. rat.

Organism-specific databases

CTD84557.
RGD735183. Map1lc3a.

Phylogenomic databases

eggNOGNOG264390.
GeneTreeENSGT00390000012937.
HOGENOMHOG000232034.
HOVERGENHBG051706.
InParanoidQ6XVN8.
KOK10435.
OMARTFADRC.
OrthoDBEOG7XH6RV.
PhylomeDBQ6XVN8.
TreeFamTF312964.

Gene expression databases

GenevestigatorQ6XVN8.

Family and domain databases

InterProIPR004241. Atg8_like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERPTHR10969. PTHR10969. 1 hit.
PfamPF02991. Atg8. 1 hit.
[Graphical view]
SUPFAMSSF54236. SSF54236. 1 hit.
ProtoNetSearch...

Other

NextBio679168.
PROQ6XVN8.

Entry information

Entry nameMLP3A_RAT
AccessionPrimary (citable) accession number: Q6XVN8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families