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Protein

Microtubule-associated proteins 1A/1B light chain 3A

Gene

Map1lc3a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei120 – 1212Cleavage; by ATG4BBy similarity

GO - Molecular functioni

  1. GABA receptor binding Source: GO_Central
  2. microtubule binding Source: GO_Central
  3. phosphatidylethanolamine binding Source: Ensembl

GO - Biological processi

  1. autophagic vacuole assembly Source: UniProtKB
  2. autophagy Source: RGD
  3. cellular response to nitrogen starvation Source: GO_Central
  4. membrane fusion Source: GO_Central
  5. mitochondrion degradation Source: GO_Central
  6. nucleophagy Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Autophagy, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated proteins 1A/1B light chain 3A
Alternative name(s):
Autophagy-related protein LC3 A
Autophagy-related ubiquitin-like modifier LC3 A
MAP1 light chain 3-like protein 1
MAP1A/MAP1B light chain 3 A
Short name:
MAP1A/MAP1B LC3 A
Microtubule-associated protein 1 light chain 3 alpha
Gene namesi
Name:Map1lc3a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 3

Organism-specific databases

RGDi735183. Map1lc3a.

Subcellular locationi

Cytoplasmcytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicleautophagosome membrane By similarity; Lipid-anchor By similarity
Note: LC3-II binds to the autophagic membranes.By similarity

GO - Cellular componenti

  1. autophagic vacuole Source: UniProtKB
  2. autophagic vacuole membrane Source: RGD
  3. cytoplasm Source: UniProtKB
  4. cytoplasmic vesicle Source: UniProtKB-KW
  5. cytosol Source: RGD
  6. extrinsic component of membrane Source: GO_Central
  7. late endosome Source: RGD
  8. microtubule Source: UniProtKB-KW
  9. organelle membrane Source: UniProtKB
  10. pre-autophagosomal structure Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 120120Microtubule-associated proteins 1A/1B light chain 3APRO_0000017196Add
BLAST
Propeptidei121 – 1211Removed in mature formCuratedPRO_0000017197

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei12 – 121Phosphoserine; by PKABy similarity
Lipidationi120 – 1201Phosphatidylethanolamine amidated glycineCurated

Post-translational modificationi

The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.1 Publication
Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ6XVN8.
PRIDEiQ6XVN8.

PTM databases

PhosphoSiteiQ6XVN8.

Expressioni

Gene expression databases

GenevestigatoriQ6XVN8.

Interactioni

Subunit structurei

3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins (By similarity). Interacts with TP53INP2 (By similarity). Interacts with TP53INP1 and SQSTM1. Directly interacts with SQSTM1; this interaction leads to MAP1LC3A recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy (By similarity). Interacts with ATG13 and ULK1 (By similarity). Interacts with TBC1D5 (By similarity).By similarity

Protein-protein interaction databases

BioGridi263296. 1 interaction.
IntActiQ6XVN8. 5 interactions.
STRINGi10116.ENSRNOP00000032429.

Structurei

3D structure databases

ProteinModelPortaliQ6XVN8.
SMRiQ6XVN8. Positions 2-121.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATG8 family.Curated

Phylogenomic databases

eggNOGiNOG264390.
GeneTreeiENSGT00390000012937.
HOGENOMiHOG000232034.
HOVERGENiHBG051706.
InParanoidiQ6XVN8.
KOiK10435.
OMAiRTFADRC.
OrthoDBiEOG7XH6RV.
PhylomeDBiQ6XVN8.
TreeFamiTF312964.

Family and domain databases

InterProiIPR004241. Atg8_like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiPF02991. Atg8. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6XVN8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPSDRPFKQR RSFADRCKEV QQIRDQHPSK IPVIIERYKG EKQLPVLDKT
60 70 80 90 100
KFLVPDHVNM SELVKIIRRR LQLNPTQAFF LLVNQHSMVS VSTPIADIYE
110 120
QEKDEDGFLY MVYASQETFG F
Length:121
Mass (Da):14,272
Last modified:July 5, 2004 - v1
Checksum:i48C1FBE8F7892AF3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY206668 mRNA. Translation: AAP42560.1.
BC086389 mRNA. Translation: AAH86389.1.
RefSeqiNP_955794.1. NM_199500.2.
UniGeneiRn.3135.

Genome annotation databases

EnsembliENSRNOT00000035060; ENSRNOP00000032429; ENSRNOG00000025443.
GeneIDi362245.
KEGGirno:362245.
UCSCiRGD:735183. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY206668 mRNA. Translation: AAP42560.1.
BC086389 mRNA. Translation: AAH86389.1.
RefSeqiNP_955794.1. NM_199500.2.
UniGeneiRn.3135.

3D structure databases

ProteinModelPortaliQ6XVN8.
SMRiQ6XVN8. Positions 2-121.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi263296. 1 interaction.
IntActiQ6XVN8. 5 interactions.
STRINGi10116.ENSRNOP00000032429.

PTM databases

PhosphoSiteiQ6XVN8.

Proteomic databases

PaxDbiQ6XVN8.
PRIDEiQ6XVN8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000035060; ENSRNOP00000032429; ENSRNOG00000025443.
GeneIDi362245.
KEGGirno:362245.
UCSCiRGD:735183. rat.

Organism-specific databases

CTDi84557.
RGDi735183. Map1lc3a.

Phylogenomic databases

eggNOGiNOG264390.
GeneTreeiENSGT00390000012937.
HOGENOMiHOG000232034.
HOVERGENiHBG051706.
InParanoidiQ6XVN8.
KOiK10435.
OMAiRTFADRC.
OrthoDBiEOG7XH6RV.
PhylomeDBiQ6XVN8.
TreeFamiTF312964.

Miscellaneous databases

NextBioi679168.
PROiQ6XVN8.

Gene expression databases

GenevestigatoriQ6XVN8.

Family and domain databases

InterProiIPR004241. Atg8_like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiPF02991. Atg8. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of rat lc3 orthologs."
    Dang Y., Yu L., Wu J., Pei Y.
    Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.
  3. Lubec G., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 31-37, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  4. "Effects of RNA interference of Atg4B on the limited proteolysis of LC3 in PC12 cells and expression of Atg4B in various rat tissues."
    Yoshimura K., Shibata M., Koike M., Gotoh K., Fukaya M., Watanabe M., Uchiyama Y.
    Autophagy 2:200-208(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE BY ATG4B.
  5. "TP53INP1, a tumor suppressor, interacts with LC3 and ATG8-family proteins through the LC3-interacting region (LIR) and promotes autophagy-dependent cell death."
    Seillier M., Peuget S., Gayet O., Gauthier C., N'guessan P., Monte M., Carrier A., Iovanna J.L., Dusetti N.J.
    Cell Death Differ. 19:1525-1535(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TP53INP1 AND SQSTM1.

Entry informationi

Entry nameiMLP3A_RAT
AccessioniPrimary (citable) accession number: Q6XVN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: July 5, 2004
Last modified: February 4, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.