Reviewed, UniProtKB/Swiss-Prot Q6XVG2 (CP254_MOUSE)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 2C54
    EC=1.14.14.1
Alternative name(s):
    CYPIIC54

Gene names

Name:

Cyp2c54

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Metabolizes arachidonic acid mainly to 12-hydroxyeicosatetraenoic acid (HETE). Ref.1
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. Ref.1
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein Ref.1.
Tissue specificity	Expressed in liver. Ref.1
Induction	P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Cytochrome P450 2C54		PRO_0000282958
Sites
	Metal binding	435	1	Iron (heme axial ligand) By similarity UniProtKB P10632

Sequences

	Sequence		Length	Mass (Da)
	Q6XVG2-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C226BBBB734DE29D	FASTA	490	55,858
	10 20 30 40 50 60 MDPILVLVLT LSCLFLLSLW RQSYERGKLP PGPTPLPIIG NILQIDVKDI CQSFTNLSRV 70 80 90 100 110 120 YGPVYTLYLG RKPTVVLHGY EAVKEALVDH GDVFAGRGRL PVFDKATNGM GIGFSNGSVW 130 140 150 160 170 180 KNTRHFSLMT LRNLGMGKRS IEDRVQEEAR CLVEELRKTN GSPCDPTFIL GCAPCNVICS 190 200 210 220 230 240 IIFQDRFDYK DRDFLNLLEK LDEISKILST PWLQVCNTFP ALLDYCPGSH NQFFKNYAYI 250 260 270 280 290 300 KNFLLEKIRE HKESLDVTIP RDFIDYFLIK GAQEDDNHPL KNNFEHLAIT VTDLFIGGTE 310 320 330 340 350 360 SMSTTLRYAL LLLLKYPHVT AKVQEEIEHV IGKHRRPCMQ DRSHMPYTNA MIHEVQRFID 370 380 390 400 410 420 LVPNNLPHEV TCDIKFRNYF IPKGTTVITS LSSVLRDSKE FPNPEKFDPG HFLDENGKFK 430 440 450 460 470 480 KSDYFMPFST GKRICAGEGL ARMELFLFLT SILQNFNLKP LVHPKDIDIT PMLIGLGSVP 490 PAFQLCFIPS

References

[1]

"Cloning, expression, and characterization of three new mouse cytochrome p450 enzymes and partial characterization of their fatty acid oxidation activities." Wang H., Zhao Y., Bradbury J.A., Graves J.P., Foley J., Blaisdell J.A., Goldstein J.A., Zeldin D.C. Mol. Pharmacol. 65:1148-1158(2004) [PubMed: 15102943] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: C57BL/6. Tissue: Liver.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY206874 mRNA. Translation: AAO52737.1.
IPI	IPI00409800.
RefSeq	NP_996260.1.
UniGene	Mm.379575
3D structure databases
HSSP	HSSP built from PDB template 1R9O based on UniProtKB P11712.
SMR	Q6XVG2. Positions 30-489.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6XVG2.
PTM databases
PhosphoSite	Q6XVG2.
Proteomic databases
PRIDE	Q6XVG2.
Genome annotation databases
Ensembl	ENSMUST00000048959; ENSMUSP00000048284; ENSMUSG00000067225; Mus musculus. [Genome view]
GeneID	404195.
KEGG	mmu:404195.
UCSC	uc008hkg.1. mouse.
Organism-specific databases
CTD	404195.
MGI	MGI:3642960. Cyp2c54.
Phylogenomic databases
eggNOG	maNOG13283.
HOGENOM	HBG749920.
HOVERGEN	Q6XVG2.
InParanoid	Q6XVG2.
OrthoDB	EOG9TTK37.
PhylomeDB	Q6XVG2.
Enzyme and pathway databases
BRENDA	1.14.14.1. 244.
Gene expression databases
ArrayExpress	Q6XVG2.
Bgee	Q6XVG2.
Genevestigator	Q6XVG2.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	406406.
SOURCE	Search...

Entry information

Entry name

CP254_MOUSE

Accession

Primary (citable) accession number: Q6XVG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	July 5, 2004
Last modified:	January 19, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)