Q6XVG2 (CP254_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2C54
EC=1.14.14.1

Alternative name(s):
CYPIIC54

Gene names

Name:

Cyp2c54

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Metabolizes arachidonic acid mainly to 12-hydroxyeicosatetraenoic acid (HETE). Ref.1
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. Ref.1
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein Ref.1.
Tissue specificity	Expressed in liver. Ref.1
Induction	P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Cytochrome P450 2C54		PRO_0000282958
Sites
	Metal binding	435	1	Iron (heme axial ligand) By similarity UniProtKB P10632

Sequences

	Sequence		Length	Mass (Da)
	Q6XVG2 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C226BBBB734DE29D	FASTA	490	55,858
	10 20 30 40 50 60 MDPILVLVLT LSCLFLLSLW RQSYERGKLP PGPTPLPIIG NILQIDVKDI CQSFTNLSRV 70 80 90 100 110 120 YGPVYTLYLG RKPTVVLHGY EAVKEALVDH GDVFAGRGRL PVFDKATNGM GIGFSNGSVW 130 140 150 160 170 180 KNTRHFSLMT LRNLGMGKRS IEDRVQEEAR CLVEELRKTN GSPCDPTFIL GCAPCNVICS 190 200 210 220 230 240 IIFQDRFDYK DRDFLNLLEK LDEISKILST PWLQVCNTFP ALLDYCPGSH NQFFKNYAYI 250 260 270 280 290 300 KNFLLEKIRE HKESLDVTIP RDFIDYFLIK GAQEDDNHPL KNNFEHLAIT VTDLFIGGTE 310 320 330 340 350 360 SMSTTLRYAL LLLLKYPHVT AKVQEEIEHV IGKHRRPCMQ DRSHMPYTNA MIHEVQRFID 370 380 390 400 410 420 LVPNNLPHEV TCDIKFRNYF IPKGTTVITS LSSVLRDSKE FPNPEKFDPG HFLDENGKFK 430 440 450 460 470 480 KSDYFMPFST GKRICAGEGL ARMELFLFLT SILQNFNLKP LVHPKDIDIT PMLIGLGSVP 490 PAFQLCFIPS

References

[1]

"Cloning, expression, and characterization of three new mouse cytochrome p450 enzymes and partial characterization of their fatty acid oxidation activities." Wang H., Zhao Y., Bradbury J.A., Graves J.P., Foley J., Blaisdell J.A., Goldstein J.A., Zeldin D.C. Mol. Pharmacol. 65:1148-1158(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: C57BL/6. Tissue: Liver.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY206874 mRNA. Translation: AAO52737.1.
IPI	IPI00409800.
RefSeq	NP_996260.1. NM_206537.2.
UniGene	Mm.379575.
3D structure databases
HSSP	HSSP built from PDB template 1R9O based on UniProtKB P11712.
ProteinModelPortal	Q6XVG2.
SMR	Q6XVG2. Positions 27-489.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000048284.
PTM databases
PhosphoSite	Q6XVG2.
Proteomic databases
PaxDb	Q6XVG2.
PRIDE	Q6XVG2.
Protocols and materials databases
DNASU	404195.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000048959; ENSMUSP00000048284; ENSMUSG00000067225.
GeneID	404195.
KEGG	mmu:404195.
Organism-specific databases
CTD	404195.
MGI	MGI:3642960. Cyp2c54.
Phylogenomic databases
eggNOG	COG2124.
GeneTree	ENSGT00680000099783.
HOGENOM	HOG000036992.
HOVERGEN	HBG015789.
InParanoid	Q6XVG2.
KO	K07413.
OrthoDB	EOG48WC22.
Gene expression databases
Bgee	Q6XVG2.
Genevestigator	Q6XVG2.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	406406.
SOURCE	Search...

Entry information

Entry name

CP254_MOUSE

Accession

Primary (citable) accession number: Q6XVG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program