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Q6XV27

- NRAM_I56A2

UniProt

Q6XV27 - NRAM_I56A2

Protein

Neuraminidase

Gene

NA

Organism
Influenza A virus (strain A/Duck/England/1/1956 H11N6)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication.

    Catalytic activityi

    Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

    Cofactori

    Binds 1 calcium ion per subunit.2 Publications

    Enzyme regulationi

    Inhibited by the neuraminidase inhibitors zanamivir (Relenza) and oseltamivir (Tamiflu). These drugs interfere with the release of progeny virus from infected cells and are effective against all influenza strains. Resistance to neuraminidase inhibitors is quite rare.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181Substrate
    Active sitei151 – 1511Proton donor/acceptorBy similarity
    Binding sitei152 – 1521Substrate
    Binding sitei293 – 2931Substrate
    Metal bindingi294 – 2941Calcium; via carbonyl oxygen2 Publications
    Metal bindingi298 – 2981Calcium; via carbonyl oxygen2 Publications
    Metal bindingi325 – 3251Calcium2 Publications
    Metal bindingi348 – 3481Calcium; via carbonyl oxygen2 Publications
    Binding sitei372 – 3721Substrate
    Active sitei406 – 4061NucleophileBy similarity

    GO - Molecular functioni

    1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
    2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
    3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Calcium, Metal-binding

    Protein family/group databases

    CAZyiGH34. Glycoside Hydrolase Family 34.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neuraminidase (EC:3.2.1.18)
    Gene namesi
    Name:NA
    OrganismiInfluenza A virus (strain A/Duck/England/1/1956 H11N6)
    Taxonomic identifieri383550 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
    Virus hostiAves [TaxID: 8782]
    ProteomesiUP000008435: Genome

    Subcellular locationi

    Virion membrane By similarity. Host apical cell membrane By similarity; Single-pass type II membrane protein By similarity
    Note: Preferentially accumulates at the apical plasma membrane in infected polarized epithelial cells, which is the virus assembly site. Uses lipid rafts for cell surface transport and apical sorting. In the virion, forms a mushroom-shaped spike on the surface of the membrane By similarity.By similarity

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 470470NeuraminidasePRO_0000280127Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi51 – 511N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi54 – 541N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi62 – 621N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi67 – 671N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi70 – 701N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi86 – 861N-linked (GlcNAc...); by host1 Publication
    Disulfide bondi92 ↔ 419
    Disulfide bondi124 ↔ 129
    Glycosylationi146 – 1461N-linked (GlcNAc...); by host2 Publications
    Disulfide bondi176 ↔ 194
    Disulfide bondi184 ↔ 231
    Glycosylationi201 – 2011N-linked (GlcNAc...); by host1 Publication
    Disulfide bondi233 ↔ 238
    Disulfide bondi279 ↔ 292
    Disulfide bondi281 ↔ 290
    Disulfide bondi319 ↔ 337
    Glycosylationi402 – 4021N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi423 ↔ 449

    Post-translational modificationi

    N-glycosylated.2 Publications

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Homotetramer.2 Publications

    Structurei

    Secondary structure

    1
    470
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi95 – 1028
    Helixi105 – 1095
    Beta strandi115 – 12511
    Beta strandi128 – 14215
    Helixi143 – 1453
    Turni146 – 1494
    Beta strandi157 – 1626
    Beta strandi173 – 18513
    Beta strandi187 – 19711
    Helixi199 – 2013
    Beta strandi203 – 2086
    Beta strandi211 – 2177
    Beta strandi219 – 2224
    Beta strandi237 – 2459
    Beta strandi247 – 2493
    Beta strandi252 – 2598
    Beta strandi262 – 2687
    Beta strandi277 – 2848
    Beta strandi287 – 2937
    Beta strandi295 – 2973
    Beta strandi302 – 3076
    Turni308 – 3114
    Beta strandi312 – 3176
    Beta strandi320 – 3223
    Beta strandi325 – 3273
    Beta strandi337 – 3393
    Beta strandi362 – 3654
    Beta strandi367 – 37913
    Turni381 – 3855
    Beta strandi392 – 40312
    Beta strandi407 – 4115
    Beta strandi415 – 4206
    Beta strandi422 – 43110
    Turni432 – 4343
    Beta strandi441 – 45313
    Helixi466 – 4694

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1V0ZX-ray1.84A/B/C/D82-470[»]
    1W1XX-ray2.00A/B/C/D82-470[»]
    1W20X-ray2.08A/B/C/D82-470[»]
    1W21X-ray2.08A/B/C/D82-470[»]
    2CMLX-ray2.15A/B/C/D82-470[»]
    ProteinModelPortaliQ6XV27.
    SMRiQ6XV27. Positions 82-470.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ6XV27.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 66IntravirionSequence Analysis
    Topological domaini28 – 470443Virion surfaceSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni11 – 3323Involved in apical transport and lipid raft associationBy similarityAdd
    BLAST
    Regioni36 – 9055Hypervariable stalk regionBy similarityAdd
    BLAST
    Regioni91 – 470380Head of neuraminidaseBy similarityAdd
    BLAST
    Regioni277 – 2782Substrate bindingBy similarity

    Domaini

    Intact N-terminus is essential for virion morphogenesis. Possess two apical sorting signals, one in the ectodomain, which is likely to be a glycan, and the other in the transmembrane domain. The transmembrane domain also plays a role in lipid raft association By similarity.By similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 34 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di2.120.10.10. 1 hit.
    InterProiIPR001860. Glyco_hydro_34.
    IPR011040. Sialidases.
    [Graphical view]
    PfamiPF00064. Neur. 1 hit.
    [Graphical view]
    SUPFAMiSSF50939. SSF50939. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q6XV27-1 [UniParc]FASTAAdd to Basket

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    MNPNQKIICI SATGMTLSVV SLLVGIANLG LNIGLHYKVG DTPNVNIPNV    50
    NGTNSTTTII NNNTQNNFTN ITNIIQSKGG ERTFLNLTKP LCEVNSWHIL 100
    SKDNAIRIGE DAHILVTREP YLSCDPQGCR MFALSQGTTL RGRHANGTIH 150
    DRSPFRALIS WEMGQAPSPY NTRVECIGWS STSCHDGMSR MSICMSGPNN 200
    NASAVVWYGG RPITEIPSWA GNILRTQESE CVCHKGVCPV VMTDGPANNR 250
    AATKIIYFKE GKIQKIEELA GNAQHIEECS CYGAGGVIKC ICRDNWKGAN 300
    RPVITIDPEM MTHTSKYLCS KVLTDTSRPN DPTNGNCDAP ITGGSPDPGV 350
    KGFAFLDGEN SWLGRTISKD SRSGYEMLKV PNAETDIQSG PISNQVIVNN 400
    QNWSGYSGAF IDYWANKECF NPCFYVELIR GRPKESSVLW TSNSIVALCG 450
    SKKRLGSWSW HDGAEIIYFE 470
    Length:470
    Mass (Da):51,470
    Last modified:July 5, 2004 - v1
    Checksum:iC3C30CB83D15E0E7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY207549 Genomic DNA. Translation: AAO62063.1.
    AB288846 Genomic RNA. Translation: BAF43436.1.
    CY014681 Genomic RNA. Translation: ABI84548.1.
    K01039 Genomic RNA. Translation: AAA43389.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY207549 Genomic DNA. Translation: AAO62063.1 .
    AB288846 Genomic RNA. Translation: BAF43436.1 .
    CY014681 Genomic RNA. Translation: ABI84548.1 .
    K01039 Genomic RNA. Translation: AAA43389.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1V0Z X-ray 1.84 A/B/C/D 82-470 [» ]
    1W1X X-ray 2.00 A/B/C/D 82-470 [» ]
    1W20 X-ray 2.08 A/B/C/D 82-470 [» ]
    1W21 X-ray 2.08 A/B/C/D 82-470 [» ]
    2CML X-ray 2.15 A/B/C/D 82-470 [» ]
    ProteinModelPortali Q6XV27.
    SMRi Q6XV27. Positions 82-470.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH34. Glycoside Hydrolase Family 34.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q6XV27.
    PROi Q6XV27.

    Family and domain databases

    Gene3Di 2.120.10.10. 1 hit.
    InterProi IPR001860. Glyco_hydro_34.
    IPR011040. Sialidases.
    [Graphical view ]
    Pfami PF00064. Neur. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50939. SSF50939. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genetic analysis of multiple N3, N4, and N6 influenza A virus neuraminidase genes."
      Webby R.J., Humberd J.L., Krauss S.L.
      Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    3. "Sequence variation at the 3' end of the neuraminidase gene from 39 influenza type A viruses."
      Blok J., Air G.M.
      Virology 121:211-229(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-71.
    4. "Assembly and budding of influenza virus."
      Nayak D.P., Hui E.K., Barman S.
      Virus Res. 106:147-165(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    5. "Neuraminidase inhibitors for influenza."
      Moscona A.
      N. Engl. J. Med. 353:1363-1373(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    6. "Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses."
      Suzuki Y.
      Biol. Pharm. Bull. 28:399-408(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    7. "The crystal structure of influenza type A virus neuraminidase of the N6 subtype at 1.85 A resolution."
      Rudino-Pinera E., Crennell S.J., Webster R.G., Laver W.G., Garman E.F.
      Submitted (APR-2004) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.84 ANGSTROMS) OF 82-470 IN COMPLEX WITH CALCIUM AND SUBSTRATE ANALOG, COFACTOR, SUBUNIT, DISULFIDE BONDS, GLYCOSYLATION AT ASN-86; ASN-146 AND ASN-201.
    8. "The crystal structure of type A influenza virus neuraminidase of the N6 subtype reveals the existence of two separate Neu5Ac binding sites."
      Rudino-Pinera E., Tunnah P., Crennell S.J., Webster R.G., Laver W.G., Garman E.F.
      Submitted (MAY-2006) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 82-470 IN COMPLEX WITH CALCIUM AND ZANAMIVIR, COFACTOR, SUBUNIT, DISULFIDE BONDS, GLYCOSYLATION AT ASN-146.

    Entry informationi

    Entry nameiNRAM_I56A2
    AccessioniPrimary (citable) accession number: Q6XV27
    Secondary accession number(s): Q83982
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 2007
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The influenza A genome consist of 8 RNA segments. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains. The mechanism of variation can be the result of point mutations or the result of genetic reassortment between segments of two different strains.

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3