Reviewed, UniProtKB/Swiss-Prot Q6XUZ5 (IDHC_SHEEP)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NADP] cytoplasmic
      Short name=IDH
    EC=1.1.1.42
Alternative name(s):
    Cytosolic NADP-isocitrate dehydrogenase
    Oxalosuccinate decarboxylase
    NADP(+)-specific ICDH
    IDP

Gene names

Name:

IDH1

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. Oxalosuccinate + NADP+ = 2-oxoglutarate + CO2 + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NADP
Molecular function	Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW isocitrate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 414	414	Isocitrate dehydrogenase [NADP] cytoplasmic		PRO_0000236189
Regions
	Nucleotide binding	75 – 77	3	NADP By similarity
	Nucleotide binding	310 – 315	6	NADP By similarity
	Region	94 – 100	7	Substrate binding By similarity
Sites
	Metal binding	252	1	Magnesium or manganese By similarity
	Metal binding	275	1	Magnesium or manganese By similarity
	Binding site	77	1	Substrate By similarity
	Binding site	82	1	NADP By similarity
	Binding site	109	1	Substrate By similarity
	Binding site	132	1	Substrate By similarity
	Binding site	260	1	NADP By similarity
	Binding site	328	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Site	139	1	Critical for catalysis By similarity
	Site	212	1	Critical for catalysis By similarity
Amino acid modifications
	Modified residue	81	1	N6-acetyllysine By similarity
	Modified residue	224	1	N6-acetyllysine By similarity
	Modified residue	321	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6XUZ5-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: B37B3D0229D94F39	FASTA	414	46,783
	10 20 30 40 50 60 MSHKIQGGSV VEMQGDEMTR IIWELIKEKL IFPYVDLDLH SYDLSIENRD ATNDQVTKDA 70 80 90 100 110 120 AEAIKKYNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR NILGGTVFRE AIICKNIPRL 130 140 150 160 170 180 VSGWVKPIII GRHAYGDQYR ATDFVVPGPG KVEICYTPSD GSPKTVYLVH NFTESGGVAM 190 200 210 220 230 240 GMFNQDKSIE DFAHSSFQMA LSKNWPLYLS TKNTILKKYD GRFKDIFQEI YDKQYKSQFE 250 260 270 280 290 300 AQNIWYEHRL IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLVCPDG 310 320 330 340 350 360 KTVEAEAAHG TVTRHYRMYQ KGQETSTNPI ASIFAWTRGL AHRAKLDNNK ELSFFAKALE 370 380 390 400 410 EVCIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK LGENLQLKLA QAKL

References

[1]	"Ovine cytosolic NADP-isocitrate dehydrogenase." Kenoutis C., Arvanitakis I., Bizelis J., Oikonomou I., Katinakis P., Rogdakis E. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY208678 mRNA. Translation: AAP41947.1.
RefSeq	NP_001009276.1.
UniGene	Oar.13049
3D structure databases
SMR	Q6XUZ5. Positions 1-414.
ModBase	Search...
Genome annotation databases
GeneID	443257.
Organism-specific databases
CTD	443257.
Phylogenomic databases
HOVERGEN	Q6XUZ5.
Enzyme and pathway databases
BRENDA	1.1.1.42. 271.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004790. Isocitrate_DH_NADP-dep_euk. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11822. IDH_NADP_euk. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMs	TIGR00127. nadp_idh_euk. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDHC_SHEEP

Accession

Primary (citable) accession number: Q6XUZ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)