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Q6XUX3 (DUSTY_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dual serine/threonine and tyrosine protein kinase

EC=2.7.12.1
Alternative name(s):
Dusty protein kinase
Short name=Dusty PK
RIP-homologous kinase
Receptor-interacting serine/threonine-protein kinase 5
Sugen kinase 496
Short name=SgK496
Gene names
Name:DSTYK
Synonyms:KIAA0472, RIP5, RIPK5, SGK496
ORF Names:HDCMD38P
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length929 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May induce both caspase-dependent apoptosis and caspase-independent cell death. Ref.6

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subcellular location

Cytoplasm Ref.1.

Tissue specificity

Predominantly expressed in skeletal muscle and testis. Weakly expressed in heart, brain, placenta, kidney, pancreas, spleen, thymus, prostate, uterus, small intestine, white blood cells, stomach, spinal cord and adrenal gland. Is widely distributed in the CNS. Also detected in several tumor cell lines. Ref.1 Ref.6

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6XUX3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6XUX3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     824-868: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q6XUX3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     451-792: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q6XUX3-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-539: Missing.
     703-718: SLPKHERLVDLHGSVI → WVLASFISMRKIQRRI
     719-929: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 929929Dual serine/threonine and tyrosine protein kinase
PRO_0000233118

Regions

Domain652 – 906255Protein kinase
Nucleotide binding658 – 6669ATP By similarity
Coiled coil189 – 21527 Potential
Coiled coil395 – 43137 Potential
Compositional bias15 – 228Poly-Gly
Compositional bias69 – 724Poly-Gly

Sites

Active site7771Proton acceptor By similarity
Binding site6811ATP By similarity

Natural variations

Alternative sequence1 – 539539Missing in isoform 4.
VSP_018030
Alternative sequence451 – 792342Missing in isoform 3.
VSP_018031
Alternative sequence703 – 71816SLPKH…HGSVI → WVLASFISMRKIQRRI in isoform 4.
VSP_018032
Alternative sequence719 – 929211Missing in isoform 4.
VSP_018033
Alternative sequence824 – 86845Missing in isoform 2.
VSP_018034
Natural variant4321L → V.
Corresponds to variant rs35845538 [ dbSNP | Ensembl ].
VAR_057101

Experimental info

Mutagenesis6811K → Q: No change. Ref.6
Sequence conflict2471V → A in AAH72406. Ref.4
Sequence conflict4491G → R in AAH60870. Ref.4
Sequence conflict6411C → R in AAP42418. Ref.1
Sequence conflict6411C → R in AAS55390. Ref.1
Sequence conflict6411C → R in AAF65505. Ref.2
Sequence conflict6411C → R in AAH53627. Ref.4
Sequence conflict6411C → R in AAH72406. Ref.4
Sequence conflict6411C → R in AAI17412. Ref.4
Sequence conflict6411C → R in AAI43604. Ref.4
Sequence conflict6411C → R in BAA32317. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 18, 2010. Version 2.
Checksum: 8DDAAC289DE21EE7

FASTA929105,206
        10         20         30         40         50         60 
MEGDGVPWGS EPVSGPGPGG GGMIRELCRG FGRYRRYLGR LRQNLRETQK FFRDIKCSHN 

        70         80         90        100        110        120 
HTCLSSLTGG GGAERGPAGD VAETGLQAGQ LSCISFPPKE EKYLQQIVDC LPCILILGQD 

       130        140        150        160        170        180 
CNVKCQLLNL LLGVQVLPTT KLGSEESCKL RRLRFTYGTQ TRVSLALPGQ YELVHTLVAH 

       190        200        210        220        230        240 
QGNWETIPEE DLEVQENNED AAHVLAELEV TMHHALLQEV DVVVAPCQGL RPTVDVLGDL 

       250        260        270        280        290        300 
VNDFLPVITY ALHKDELSER DEQELQEIRK YFSFPVFFFK VPKLGSEIID SSTRRMESER 

       310        320        330        340        350        360 
SPLYRQLIDL GYLSSSHWNC GAPGQDTKAQ SMLVEQSEKL RHLSTFSHQV LQTRLVDAAK 

       370        380        390        400        410        420 
ALNLVHCHCL DIFINQAFDM QRDLQITPKR LEYTRKKENE LYESLMNIAN RKQEEMKDMI 

       430        440        450        460        470        480 
VETLNTMKEE LLDDATNMEF KDVIVPENGE PVGTREIKCC IRQIQELIIS RLNQAVANKL 

       490        500        510        520        530        540 
ISSVDYLRES FVGTLERCLQ SLEKSQDVSV HITSNYLKQI LNAAYHVEVT FHSGSSVTRM 

       550        560        570        580        590        600 
LWEQIKQIIQ RITWVSPPAI TLEWKRKVAQ EAIESLSASK LAKSICSQFR TRLNSSHEAF 

       610        620        630        640        650        660 
AASLRQLEAG HSGRLEKTED LWLRVRKDHA PRLARLSLES CSLQDVLLHR KPKLGQELGR 

       670        680        690        700        710        720 
GQYGVVYLCD NWGGHFPCAL KSVVPPDEKH WNDLALEFHY MRSLPKHERL VDLHGSVIDY 

       730        740        750        760        770        780 
NYGGGSSIAV LLIMERLHRD LYTGLKAGLT LETRLQIALD VVEGIRFLHS QGLVHRDIKL 

       790        800        810        820        830        840 
KNVLLDKQNR AKITDLGFCK PEAMMSGSIV GTPIHMAPEL FTGKYDNSVD VYAFGILFWY 

       850        860        870        880        890        900 
ICSGSVKLPE AFERCASKDH LWNNVRRGAR PERLPVFDEE CWQLMEACWD GDPLKRPLLG 

       910        920 
IVQPMLQGIM NRLCKSNSEQ PNRGLDDST 

« Hide

Isoform 2 [UniParc].

Checksum: 89E487C2D3CDD2FA
Show »

FASTA88499,968
Isoform 3 [UniParc].

Checksum: 6C7E99A983B9BA58
Show »

FASTA58766,302
Isoform 4 [UniParc].

Checksum: 67DB6F18452BB99F
Show »

FASTA17920,769

References

« Hide 'large scale' references
[1]"Dusty protein kinases: primary structure, gene evolution, tissue specific expression and unique features of the catalytic domain."
Peng J., Dong W., Chen Y., Mo R., Cheng J.-F., Hui C.-C., Mohandas N., Huang C.-H.
Biochim. Biophys. Acta 1759:562-572(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Brain.
[2]"A novel gene from human dendritic cell."
Zhao Z., Huang X., Li N., Zhu X., Cao X.
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
Tissue: Dendritic cell.
[3]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
Tissue: Brain, Eye, Muscle, Placenta and Skin.
[5]"Characterization of cDNA clones in size-fractionated cDNA libraries from human brain."
Seki N., Ohira M., Nagase T., Ishikawa K., Miyajima N., Nakajima D., Nomura N., Ohara O.
DNA Res. 4:345-349(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 565-929 (ISOFORM 1).
Tissue: Brain.
[6]"RIP5 is a RIP-homologous inducer of cell death."
Zha J., Zhou Q., Xu L.G., Chen D., Li L., Zhai Z., Shu H.B.
Biochem. Biophys. Res. Commun. 319:298-303(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF LYS-681.
[7]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Large-scale proteomics analysis of the human kinome."
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H.
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY208850 mRNA. Translation: AAP42418.1.
AY429674 mRNA. Translation: AAS55390.1.
AF068286 mRNA. Translation: AAF65505.1.
AC093422 Genomic DNA. No translation available.
BC048204 mRNA. Translation: AAH48204.1.
BC053627 mRNA. Translation: AAH53627.1.
BC060870 mRNA. Translation: AAH60870.2.
BC072406 mRNA. Translation: AAH72406.1.
BC117411 mRNA. Translation: AAI17412.1.
BC143603 mRNA. Translation: AAI43604.1.
AB007941 mRNA. Translation: BAA32317.1.
CCDSCCDS1451.1. [Q6XUX3-1]
CCDS1452.1. [Q6XUX3-2]
RefSeqNP_056190.1. NM_015375.2. [Q6XUX3-1]
NP_955749.1. NM_199462.2. [Q6XUX3-2]
UniGeneHs.6874.

3D structure databases

ProteinModelPortalQ6XUX3.
SMRQ6XUX3. Positions 646-912.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid117313. 13 interactions.

Chemistry

BindingDBQ6XUX3.
ChEMBLCHEMBL1908386.
GuidetoPHARMACOLOGY2008.

PTM databases

PhosphoSiteQ6XUX3.

Polymorphism databases

DMDM296434486.

Proteomic databases

MaxQBQ6XUX3.
PaxDbQ6XUX3.
PRIDEQ6XUX3.

Protocols and materials databases

DNASU25778.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000367161; ENSP00000356129; ENSG00000133059. [Q6XUX3-2]
ENST00000367162; ENSP00000356130; ENSG00000133059. [Q6XUX3-1]
GeneID25778.
KEGGhsa:25778.
UCSCuc001hbw.3. human. [Q6XUX3-1]
uc001hbx.3. human. [Q6XUX3-2]
uc001hby.1. human. [Q6XUX3-4]

Organism-specific databases

CTD25778.
GeneCardsGC01M205111.
H-InvDBHIX0018906.
HGNCHGNC:29043. DSTYK.
HPAHPA044140.
MIM612666. gene.
neXtProtNX_Q6XUX3.
PharmGKBPA164718861.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0515.
InParanoidQ6XUX3.
KOK16288.
OMAAGQLSCI.
OrthoDBEOG79SDWH.
PhylomeDBQ6XUX3.
TreeFamTF331821.

Enzyme and pathway databases

SignaLinkQ6XUX3.

Gene expression databases

ArrayExpressQ6XUX3.
BgeeQ6XUX3.
CleanExHS_DSTYK.
GenevestigatorQ6XUX3.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDSTYK. human.
GeneWikiRIPK5.
GenomeRNAi25778.
NextBio46924.
PROQ6XUX3.
SOURCESearch...

Entry information

Entry nameDUSTY_HUMAN
AccessionPrimary (citable) accession number: Q6XUX3
Secondary accession number(s): B7ZL64 expand/collapse secondary AC list , O75060, Q17R94, Q5RKT0, Q6IN87, Q6P997, Q86Y03, Q9P1S5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 18, 2010
Last modified: July 9, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM