Reviewed, UniProtKB/Swiss-Prot Q6XUX2 (DUSTY_RAT)

Names and origin

Protein names

Recommended name:
    Dual serine/threonine and tyrosine protein kinase
    EC=2.7.12.1
Alternative name(s):
    Dusty protein kinase
      Short name=Dusty PK
    Receptor-interacting serine/threonine-protein kinase 5

Gene names

Name:	Dstyk
Synonyms:	Ripk5

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	927 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May induce both caspase-dependent apoptosis and caspase-independent cell death By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Expressed in many regions of the adult brain. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 927	927	Dual serine/threonine and tyrosine protein kinase		PRO_0000233121
Regions
	Domain	650 – 904	255	Protein kinase
	Nucleotide binding	656 – 664	9	ATP By similarity
	Coiled coil	393 – 429	37	Potential
	Compositional bias	15 – 22	8	Poly-Gly
Sites
	Active site	775	1	Proton acceptor By similarity
	Binding site	679	1	ATP By similarity
Amino acid modifications
	Modified residue	926	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6XUX2-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 88C938259D04472C	FASTA	927	104,898
	10 20 30 40 50 60 MEADGQSWSG ESVSGPGPGG GGMIRELCRG FGRYRRYLGR LRQNLRETQK FFRDIKCSHS 70 80 90 100 110 120 HSCPSSPAGG GAAELGPAGD VAAAPLPAGQ LSCISFPPME EKYLQQIVDH LPCILILGQD 130 140 150 160 170 180 CNAKCQLLNL LLAVRVLPTL KLDSDESCKL RRLRFTYGTR TQVSLALPGQ YELVHTLASH 190 200 210 220 230 240 QDNWETIPEE DLEVQEDSED TARVLADLEV TMHHALLQEV DIVVAPCHSH RPAVDVLSDL 250 260 270 280 290 300 ANDFLPVITY ALHKDELSER DEQELQEIRK YFSFPVFFFK VPTLEIIRSS SGRADSERSP 310 320 330 340 350 360 LSGQLMDLGY LSSSHRNCMA SDQDCRAQSM LVEQSEKLRQ LSTFSHQLLQ TRLVDAAKAL 370 380 390 400 410 420 NAVHSHCLDI FINQAFDMQR DLQITPKRLE YTRKKENELY ESLMNIANRK QEEMKDMIVE 430 440 450 460 470 480 TLNTMKEELL DDAANMEFKD VIVPENGETV GTREIKSCIR QIQELIISRL NQAVANKLIS 490 500 510 520 530 540 SVDYLRESFV GTLERCLQSL EKSQDVSVHI TSNYLKQILN AAYHVEVTFH SGSSVTRMLW 550 560 570 580 590 600 EQIKQIIQRI TWVNPPTITL EWKRKVAQEA IDSLSASKLA KSICSQFRTR LNSSHEAFAA 610 620 630 640 650 660 SLRQLEAGHS GRLEKTEDLW LKVRKDHAPR LARLSLESRS LQDVLLHRKP KLGQELGRGQ 670 680 690 700 710 720 YGVVYLCDNW GGHFPCALKS VVPPDEKHWN DLALEFHYMR SLPKHERLVD LHGSVIDYNY 730 740 750 760 770 780 GGGSSVAVLL IMERLHRDLY TGLKAGLSLE TRLQIALDVV EGIRFLHSQG LVHRDIKLKN 790 800 810 820 830 840 VLLDKQNRAK ITDLGFCKPE AMMSGSIVGT PIHMAPELFT GKYDNSVDVY AFGILFWYIC 850 860 870 880 890 900 SGSIKLPEAF ERCASKDHLW NNVRRGTRPE RLPVFDEECW QLMEACWDGD PSKRPLLGIV 910 920 QPILRSIMDR LCKCDSERPS RGLDDST

References

[1]	"Dusty protein kinases: primary structure, gene evolution, tissue specific expression and unique features of the catalytic domain." Peng J., Dong W., Chen Y., Mo R., Cheng J.-F., Hui C.-C., Mohandas N., Huang C.-H. Biochim. Biophys. Acta 1759:562-572(2006) [PubMed: 17123648] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: Sprague-Dawley. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY208851 mRNA. Translation: AAP42419.1. AY429675 mRNA. Translation: AAS55391.1.
IPI	IPI00371973.
RefSeq	NP_955750.1.
UniGene	Rn.32539
3D structure databases
SMR	Q6XUX2. Positions 649-907.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000004810; ENSRNOP00000004810; ENSRNOG00000021298; Rattus norvegicus. [Genome view]
GeneID	304791.
KEGG	rno:304791.
UCSC	NM_199463. rat.
Organism-specific databases
CTD	304791.
RGD	735051. Ripk5.
Phylogenomic databases
eggNOG	roNOG07193.
HOVERGEN	Q6XUX2.
InParanoid	Q6XUX2.
OMA	AGQLSCI.
OrthoDB	EOG9T7BPV.
PhylomeDB	Q6XUX2.
Enzyme and pathway databases
BRENDA	2.7.11.1. 248.
Gene expression databases
ArrayExpress	Q6XUX2.
Genevestigator	Q6XUX2.
GermOnline	ENSRNOG00000021298. Rattus norvegicus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	653620.

Entry information

Entry name

DUSTY_RAT

Accession

Primary (citable) accession number: Q6XUX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)