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Protein

Dual serine/threonine and tyrosine protein kinase

Gene

Dstyk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a positive regulator of ERK phosphorylation downstream of fibroblast growth factor-receptor activation. May induce both caspase-dependent apoptosis and caspase-independent cell death.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei679 – 6791ATPPROSITE-ProRule annotation
Active sitei775 – 7751Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi656 – 6649ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dual serine/threonine and tyrosine protein kinase (EC:2.7.12.1)
Alternative name(s):
Dusty protein kinase
Short name:
Dusty PK
Receptor-interacting serine/threonine-protein kinase 5
Gene namesi
Name:Dstyk
Synonyms:Ripk5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1925064. Dstyk.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB
  • basolateral plasma membrane Source: UniProtKB
  • cell junction Source: UniProtKB-SubCell
  • cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 927927Dual serine/threonine and tyrosine protein kinasePRO_0000233119Add
BLAST

Proteomic databases

MaxQBiQ6XUX1.
PaxDbiQ6XUX1.
PRIDEiQ6XUX1.

PTM databases

PhosphoSiteiQ6XUX1.

Expressioni

Tissue specificityi

Expressed in brain, heart, skeletal muscle, kidney and lung. Expressed in maturing tubular epithelia, with the most prominent expression in the medulla and the papilla. Expressed in thin ascending limb of the loop of Henle and the distal convoluted tubule. Expressed in all layers of transitional ureteric epithelium and in the ureteric smooth-muscle cells (at protein level). Widely expressed. Highly expressed in many brain regions, including in cerebellum, olfactory, hippocampus and cerebral cortex.2 Publications

Developmental stagei

At E14.5 detected in lung and skeletal muscle, and by E18.5 detected in skin, whisker, gut and testis.1 Publication

Gene expression databases

BgeeiQ6XUX1.
CleanExiMM_RIPK5.
ExpressionAtlasiQ6XUX1. baseline and differential.
GenevisibleiQ6XUX1. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035358.

Structurei

3D structure databases

ProteinModelPortaliQ6XUX1.
SMRiQ6XUX1. Positions 113-139, 547-910.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini650 – 904255Protein kinasePROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili393 – 42937Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi15 – 228Poly-Gly
Compositional biasi288 – 2914Poly-Ser

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0192. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00840000129948.
HOGENOMiHOG000007092.
InParanoidiQ6XUX1.
KOiK16288.
OMAiPVFFFKV.
OrthoDBiEOG79SDWH.
PhylomeDBiQ6XUX1.
TreeFamiTF331821.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6XUX1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEADGQSWAG ESVSGPGPGG GGMIRELCRG FSRYRRYLGR LRQNLRETQK
60 70 80 90 100
FFRDIKCSHS HSCPSSPAGG GAAELGPAGD VAEAPLPAGQ LSCISFPPME
110 120 130 140 150
ETYLQQLVDR LPCILILGQD CNAKCQLLNL LLGVQVLPTL KLDSDESCKL
160 170 180 190 200
RRLRFTYGTR TRVSLALPGQ YELVHTLASH QDNWETIPEE DLEVQEDSED
210 220 230 240 250
AAHVLADLEV TMHHALLQEV DIVVAPCPSH RPSVDVLSDL ANDFLPVITY
260 270 280 290 300
ALHKDELSER GEQELREVRQ YFSFPMFFFK VPKLEIISSS SGRAESERSP
310 320 330 340 350
LYGQLVDLGY LSSSHRNCVP SDQDCKAQSM LVEQSEKLKQ LSTFSHQLLQ
360 370 380 390 400
NRLVDAAKAL NVVHSHCLDI FINQAFDMQR DLQITPKRLE YTRKKENELY
410 420 430 440 450
ESLMNIANRK QEEMKDMIVE TLNTMKEELL DDAANMEFKD VIVPENGETI
460 470 480 490 500
GTREIKSCIR QIQELIISRL NQAVANKLIS SVDYLRESFV GTLERCLQSL
510 520 530 540 550
EKSQDVSVHI TSNYLKQILN AAYHVEVTFH SGSSVTRMLW EQIKQIIQRI
560 570 580 590 600
TWVNPPTITL EWKRKVAQEA IDSLSASKLA KSICSQFRTR LNSSHEAFAA
610 620 630 640 650
SLRQLEAGHS GRLEKTEDLW LKVRKDHAPR LARLSLESRS LQDVLLHRKP
660 670 680 690 700
KLGQELGRGQ YGVVYLCDNW GGHFPCALKS VVPPDEKHWN DLALEFHYMR
710 720 730 740 750
SLPKHERLVD LHGSVIDYNY GGGSSVAVLL IMERLHRDLY TGLKAGLTLE
760 770 780 790 800
TRLQIALDVV EGIRFLHSQG LVHRDIKLKN VLLDKQNRAK ITDLGFCKPE
810 820 830 840 850
AMMSGSIVGT PIHMAPELFT GKYDNSVDVY AFGILFWYIC SGSIKLPEAF
860 870 880 890 900
ERCASKDHLW NNVRRGTRPE RLPVFDEECW QLMEACWDGD PLKRPLLGIV
910 920
QPMLRSIMDR LCKCSSEQPN RGLDDST
Length:927
Mass (Da):104,901
Last modified:July 5, 2004 - v1
Checksum:iEF124A73F3509A71
GO
Isoform 2 (identifier: Q6XUX1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     923-927: Missing.

Note: No experimental confirmation available.
Show »
Length:922
Mass (Da):104,369
Checksum:i409A71F58BDBEC1B
GO
Isoform 3 (identifier: Q6XUX1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     210-218: Missing.

Note: No experimental confirmation available.
Show »
Length:918
Mass (Da):103,870
Checksum:i6A1476C7A9A9E505
GO
Isoform 4 (identifier: Q6XUX1-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-536: Missing.
     537-544: RMLWEQIK → MISLSSSW
     701-927: Missing.

Note: No experimental confirmation available.
Show »
Length:164
Mass (Da):18,814
Checksum:i20814A1ABE0AC58A
GO

Sequence cautioni

The sequence BAC34316.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAE26444.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti695 – 6951E → K in BAC31487 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 536536Missing in isoform 4. 1 PublicationVSP_018037Add
BLAST
Alternative sequencei210 – 2189Missing in isoform 3. 1 PublicationVSP_018036
Alternative sequencei537 – 5448RMLWEQIK → MISLSSSW in isoform 4. 1 PublicationVSP_018038
Alternative sequencei701 – 927227Missing in isoform 4. 1 PublicationVSP_018039Add
BLAST
Alternative sequencei923 – 9275Missing in isoform 2. 1 PublicationVSP_018035

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY208852 mRNA. Translation: AAP42420.1.
AY429676 mRNA. Translation: AAS55392.1.
AK020880 mRNA. Translation: BAB32238.1.
AK043199 mRNA. Translation: BAC31487.1.
AK050542 mRNA. Translation: BAC34316.1. Different initiation.
AK145449 mRNA. Translation: BAE26444.1. Different initiation.
BC089380 mRNA. Translation: AAH89380.1.
CCDSiCCDS15285.1. [Q6XUX1-1]
RefSeqiNP_766104.2. NM_172516.4. [Q6XUX1-1]
UniGeneiMm.257120.
Mm.421296.

Genome annotation databases

EnsembliENSMUST00000045110; ENSMUSP00000035358; ENSMUSG00000042046. [Q6XUX1-1]
GeneIDi213452.
KEGGimmu:213452.
UCSCiuc007cou.1. mouse. [Q6XUX1-1]
uc007cov.1. mouse. [Q6XUX1-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY208852 mRNA. Translation: AAP42420.1.
AY429676 mRNA. Translation: AAS55392.1.
AK020880 mRNA. Translation: BAB32238.1.
AK043199 mRNA. Translation: BAC31487.1.
AK050542 mRNA. Translation: BAC34316.1. Different initiation.
AK145449 mRNA. Translation: BAE26444.1. Different initiation.
BC089380 mRNA. Translation: AAH89380.1.
CCDSiCCDS15285.1. [Q6XUX1-1]
RefSeqiNP_766104.2. NM_172516.4. [Q6XUX1-1]
UniGeneiMm.257120.
Mm.421296.

3D structure databases

ProteinModelPortaliQ6XUX1.
SMRiQ6XUX1. Positions 113-139, 547-910.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035358.

PTM databases

PhosphoSiteiQ6XUX1.

Proteomic databases

MaxQBiQ6XUX1.
PaxDbiQ6XUX1.
PRIDEiQ6XUX1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045110; ENSMUSP00000035358; ENSMUSG00000042046. [Q6XUX1-1]
GeneIDi213452.
KEGGimmu:213452.
UCSCiuc007cou.1. mouse. [Q6XUX1-1]
uc007cov.1. mouse. [Q6XUX1-4]

Organism-specific databases

CTDi25778.
MGIiMGI:1925064. Dstyk.

Phylogenomic databases

eggNOGiKOG0192. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00840000129948.
HOGENOMiHOG000007092.
InParanoidiQ6XUX1.
KOiK16288.
OMAiPVFFFKV.
OrthoDBiEOG79SDWH.
PhylomeDBiQ6XUX1.
TreeFamiTF331821.

Miscellaneous databases

ChiTaRSiDstyk. mouse.
NextBioi373980.
PROiQ6XUX1.
SOURCEiSearch...

Gene expression databases

BgeeiQ6XUX1.
CleanExiMM_RIPK5.
ExpressionAtlasiQ6XUX1. baseline and differential.
GenevisibleiQ6XUX1. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Dusty protein kinases: primary structure, gene evolution, tissue specific expression and unique features of the catalytic domain."
    Peng J., Dong W., Chen Y., Mo R., Cheng J.-F., Hui C.-C., Mohandas N., Huang C.-H.
    Biochim. Biophys. Acta 1759:562-572(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: BALB/cJ.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-392 (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 535-927 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Cerebellum, Pancreas and Retina.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 478-927 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Eye.
  4. Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiDUSTY_MOUSE
AccessioniPrimary (citable) accession number: Q6XUX1
Secondary accession number(s): Q3ULK4
, Q5EBN5, Q8C7D4, Q8C923, Q9CTP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: July 5, 2004
Last modified: May 11, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.