Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dual serine/threonine and tyrosine protein kinase

Gene

Dstyk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a positive regulator of ERK phosphorylation downstream of fibroblast growth factor-receptor activation. May induce both caspase-dependent apoptosis and caspase-independent cell death.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei679ATPPROSITE-ProRule annotation1
Active sitei775Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi656 – 664ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dual serine/threonine and tyrosine protein kinase (EC:2.7.12.1)
Alternative name(s):
Dusty protein kinase
Short name:
Dusty PK
Receptor-interacting serine/threonine-protein kinase 5
Gene namesi
Name:Dstyk
Synonyms:Ripk5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1925064. Dstyk.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB
  • basolateral plasma membrane Source: UniProtKB
  • cell junction Source: UniProtKB-SubCell
  • cytoplasm Source: UniProtKB
  • cytosol Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002331191 – 927Dual serine/threonine and tyrosine protein kinaseAdd BLAST927

Proteomic databases

PaxDbiQ6XUX1.
PRIDEiQ6XUX1.

PTM databases

PhosphoSitePlusiQ6XUX1.

Expressioni

Tissue specificityi

Expressed in brain, heart, skeletal muscle, kidney and lung. Expressed in maturing tubular epithelia, with the most prominent expression in the medulla and the papilla. Expressed in thin ascending limb of the loop of Henle and the distal convoluted tubule. Expressed in all layers of transitional ureteric epithelium and in the ureteric smooth-muscle cells (at protein level). Widely expressed. Highly expressed in many brain regions, including in cerebellum, olfactory, hippocampus and cerebral cortex.2 Publications

Developmental stagei

At E14.5 detected in lung and skeletal muscle, and by E18.5 detected in skin, whisker, gut and testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000042046.
CleanExiMM_RIPK5.
ExpressionAtlasiQ6XUX1. baseline and differential.
GenevisibleiQ6XUX1. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035358.

Structurei

3D structure databases

ProteinModelPortaliQ6XUX1.
SMRiQ6XUX1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini650 – 904Protein kinasePROSITE-ProRule annotationAdd BLAST255

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili393 – 429Sequence analysisAdd BLAST37

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi15 – 22Poly-Gly8
Compositional biasi288 – 291Poly-Ser4

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0192. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00840000129948.
HOGENOMiHOG000007092.
InParanoidiQ6XUX1.
KOiK16288.
OMAiPVFFFKV.
OrthoDBiEOG091G03P4.
PhylomeDBiQ6XUX1.
TreeFamiTF331821.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6XUX1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEADGQSWAG ESVSGPGPGG GGMIRELCRG FSRYRRYLGR LRQNLRETQK
60 70 80 90 100
FFRDIKCSHS HSCPSSPAGG GAAELGPAGD VAEAPLPAGQ LSCISFPPME
110 120 130 140 150
ETYLQQLVDR LPCILILGQD CNAKCQLLNL LLGVQVLPTL KLDSDESCKL
160 170 180 190 200
RRLRFTYGTR TRVSLALPGQ YELVHTLASH QDNWETIPEE DLEVQEDSED
210 220 230 240 250
AAHVLADLEV TMHHALLQEV DIVVAPCPSH RPSVDVLSDL ANDFLPVITY
260 270 280 290 300
ALHKDELSER GEQELREVRQ YFSFPMFFFK VPKLEIISSS SGRAESERSP
310 320 330 340 350
LYGQLVDLGY LSSSHRNCVP SDQDCKAQSM LVEQSEKLKQ LSTFSHQLLQ
360 370 380 390 400
NRLVDAAKAL NVVHSHCLDI FINQAFDMQR DLQITPKRLE YTRKKENELY
410 420 430 440 450
ESLMNIANRK QEEMKDMIVE TLNTMKEELL DDAANMEFKD VIVPENGETI
460 470 480 490 500
GTREIKSCIR QIQELIISRL NQAVANKLIS SVDYLRESFV GTLERCLQSL
510 520 530 540 550
EKSQDVSVHI TSNYLKQILN AAYHVEVTFH SGSSVTRMLW EQIKQIIQRI
560 570 580 590 600
TWVNPPTITL EWKRKVAQEA IDSLSASKLA KSICSQFRTR LNSSHEAFAA
610 620 630 640 650
SLRQLEAGHS GRLEKTEDLW LKVRKDHAPR LARLSLESRS LQDVLLHRKP
660 670 680 690 700
KLGQELGRGQ YGVVYLCDNW GGHFPCALKS VVPPDEKHWN DLALEFHYMR
710 720 730 740 750
SLPKHERLVD LHGSVIDYNY GGGSSVAVLL IMERLHRDLY TGLKAGLTLE
760 770 780 790 800
TRLQIALDVV EGIRFLHSQG LVHRDIKLKN VLLDKQNRAK ITDLGFCKPE
810 820 830 840 850
AMMSGSIVGT PIHMAPELFT GKYDNSVDVY AFGILFWYIC SGSIKLPEAF
860 870 880 890 900
ERCASKDHLW NNVRRGTRPE RLPVFDEECW QLMEACWDGD PLKRPLLGIV
910 920
QPMLRSIMDR LCKCSSEQPN RGLDDST
Length:927
Mass (Da):104,901
Last modified:July 5, 2004 - v1
Checksum:iEF124A73F3509A71
GO
Isoform 2 (identifier: Q6XUX1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     923-927: Missing.

Note: No experimental confirmation available.
Show »
Length:922
Mass (Da):104,369
Checksum:i409A71F58BDBEC1B
GO
Isoform 3 (identifier: Q6XUX1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     210-218: Missing.

Note: No experimental confirmation available.
Show »
Length:918
Mass (Da):103,870
Checksum:i6A1476C7A9A9E505
GO
Isoform 4 (identifier: Q6XUX1-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-536: Missing.
     537-544: RMLWEQIK → MISLSSSW
     701-927: Missing.

Note: No experimental confirmation available.
Show »
Length:164
Mass (Da):18,814
Checksum:i20814A1ABE0AC58A
GO

Sequence cautioni

The sequence BAC34316 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE26444 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti695E → K in BAC31487 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0180371 – 536Missing in isoform 4. 1 PublicationAdd BLAST536
Alternative sequenceiVSP_018036210 – 218Missing in isoform 3. 1 Publication9
Alternative sequenceiVSP_018038537 – 544RMLWEQIK → MISLSSSW in isoform 4. 1 Publication8
Alternative sequenceiVSP_018039701 – 927Missing in isoform 4. 1 PublicationAdd BLAST227
Alternative sequenceiVSP_018035923 – 927Missing in isoform 2. 1 Publication5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY208852 mRNA. Translation: AAP42420.1.
AY429676 mRNA. Translation: AAS55392.1.
AK020880 mRNA. Translation: BAB32238.1.
AK043199 mRNA. Translation: BAC31487.1.
AK050542 mRNA. Translation: BAC34316.1. Different initiation.
AK145449 mRNA. Translation: BAE26444.1. Different initiation.
BC089380 mRNA. Translation: AAH89380.1.
CCDSiCCDS15285.1. [Q6XUX1-1]
RefSeqiNP_766104.2. NM_172516.4. [Q6XUX1-1]
UniGeneiMm.257120.
Mm.421296.

Genome annotation databases

EnsembliENSMUST00000045110; ENSMUSP00000035358; ENSMUSG00000042046. [Q6XUX1-1]
GeneIDi213452.
KEGGimmu:213452.
UCSCiuc007cou.1. mouse. [Q6XUX1-1]
uc007cov.1. mouse. [Q6XUX1-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY208852 mRNA. Translation: AAP42420.1.
AY429676 mRNA. Translation: AAS55392.1.
AK020880 mRNA. Translation: BAB32238.1.
AK043199 mRNA. Translation: BAC31487.1.
AK050542 mRNA. Translation: BAC34316.1. Different initiation.
AK145449 mRNA. Translation: BAE26444.1. Different initiation.
BC089380 mRNA. Translation: AAH89380.1.
CCDSiCCDS15285.1. [Q6XUX1-1]
RefSeqiNP_766104.2. NM_172516.4. [Q6XUX1-1]
UniGeneiMm.257120.
Mm.421296.

3D structure databases

ProteinModelPortaliQ6XUX1.
SMRiQ6XUX1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035358.

PTM databases

PhosphoSitePlusiQ6XUX1.

Proteomic databases

PaxDbiQ6XUX1.
PRIDEiQ6XUX1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045110; ENSMUSP00000035358; ENSMUSG00000042046. [Q6XUX1-1]
GeneIDi213452.
KEGGimmu:213452.
UCSCiuc007cou.1. mouse. [Q6XUX1-1]
uc007cov.1. mouse. [Q6XUX1-4]

Organism-specific databases

CTDi25778.
MGIiMGI:1925064. Dstyk.

Phylogenomic databases

eggNOGiKOG0192. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00840000129948.
HOGENOMiHOG000007092.
InParanoidiQ6XUX1.
KOiK16288.
OMAiPVFFFKV.
OrthoDBiEOG091G03P4.
PhylomeDBiQ6XUX1.
TreeFamiTF331821.

Miscellaneous databases

ChiTaRSiDstyk. mouse.
PROiQ6XUX1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042046.
CleanExiMM_RIPK5.
ExpressionAtlasiQ6XUX1. baseline and differential.
GenevisibleiQ6XUX1. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDUSTY_MOUSE
AccessioniPrimary (citable) accession number: Q6XUX1
Secondary accession number(s): Q3ULK4
, Q5EBN5, Q8C7D4, Q8C923, Q9CTP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.