Reviewed, UniProtKB/Swiss-Prot Q6XUX1 (DUSTY_MOUSE)

Names and origin

Protein names

Recommended name:
    Dual serine/threonine and tyrosine protein kinase
    EC=2.7.12.1
Alternative name(s):
    Dusty protein kinase
      Short name=Dusty PK
    Receptor-interacting serine/threonine-protein kinase 5

Gene names

Name:	Dstyk
Synonyms:	Ripk5

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	927 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May induce both caspase-dependent apoptosis and caspase-independent cell death By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Expressed in brain, heart, skeletal muscle, kidney and lung (at protein level). Widely expressed. Highly expressed in many brain regions, including in cerebellum, olfactory, hippocampus and cerebral cortex. Ref.1
Developmental stage	At E14.5 detected in lung and skeletal muscle, and by E18.5 detected in skin, whisker, gut and testis. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6XUX1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6XUX1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     923-927: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q6XUX1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     210-218: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q6XUX1-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-536: Missing.
     537-544: RMLWEQIK → MISLSSSW
     701-927: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 927	927	Dual serine/threonine and tyrosine protein kinase		PRO_0000233119
Regions
	Domain	650 – 904	255	Protein kinase
	Nucleotide binding	656 – 664	9	ATP By similarity
	Coiled coil	393 – 429	37	Potential
	Compositional bias	15 – 22	8	Poly-Gly
	Compositional bias	288 – 291	4	Poly-Ser
Sites
	Active site	775	1	Proton acceptor By similarity
	Binding site	679	1	ATP By similarity
Natural variations
	Alternative sequence	1 – 536	536	Missing in isoform 4.		VSP_018037
	Alternative sequence	210 – 218	9	Missing in isoform 3.		VSP_018036
	Alternative sequence	537 – 544	8	RMLWEQIK → MISLSSSW in isoform 4.		VSP_018038
	Alternative sequence	701 – 927	227	Missing in isoform 4.		VSP_018039
	Alternative sequence	923 – 927	5	Missing in isoform 2.		VSP_018035
Experimental info
	Sequence conflict	695	1	E → K in BAC31487. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: EF124A73F3509A71	FASTA	927	104,901
	10 20 30 40 50 60 MEADGQSWAG ESVSGPGPGG GGMIRELCRG FSRYRRYLGR LRQNLRETQK FFRDIKCSHS 70 80 90 100 110 120 HSCPSSPAGG GAAELGPAGD VAEAPLPAGQ LSCISFPPME ETYLQQLVDR LPCILILGQD 130 140 150 160 170 180 CNAKCQLLNL LLGVQVLPTL KLDSDESCKL RRLRFTYGTR TRVSLALPGQ YELVHTLASH 190 200 210 220 230 240 QDNWETIPEE DLEVQEDSED AAHVLADLEV TMHHALLQEV DIVVAPCPSH RPSVDVLSDL 250 260 270 280 290 300 ANDFLPVITY ALHKDELSER GEQELREVRQ YFSFPMFFFK VPKLEIISSS SGRAESERSP 310 320 330 340 350 360 LYGQLVDLGY LSSSHRNCVP SDQDCKAQSM LVEQSEKLKQ LSTFSHQLLQ NRLVDAAKAL 370 380 390 400 410 420 NVVHSHCLDI FINQAFDMQR DLQITPKRLE YTRKKENELY ESLMNIANRK QEEMKDMIVE 430 440 450 460 470 480 TLNTMKEELL DDAANMEFKD VIVPENGETI GTREIKSCIR QIQELIISRL NQAVANKLIS 490 500 510 520 530 540 SVDYLRESFV GTLERCLQSL EKSQDVSVHI TSNYLKQILN AAYHVEVTFH SGSSVTRMLW 550 560 570 580 590 600 EQIKQIIQRI TWVNPPTITL EWKRKVAQEA IDSLSASKLA KSICSQFRTR LNSSHEAFAA 610 620 630 640 650 660 SLRQLEAGHS GRLEKTEDLW LKVRKDHAPR LARLSLESRS LQDVLLHRKP KLGQELGRGQ 670 680 690 700 710 720 YGVVYLCDNW GGHFPCALKS VVPPDEKHWN DLALEFHYMR SLPKHERLVD LHGSVIDYNY 730 740 750 760 770 780 GGGSSVAVLL IMERLHRDLY TGLKAGLTLE TRLQIALDVV EGIRFLHSQG LVHRDIKLKN 790 800 810 820 830 840 VLLDKQNRAK ITDLGFCKPE AMMSGSIVGT PIHMAPELFT GKYDNSVDVY AFGILFWYIC 850 860 870 880 890 900 SGSIKLPEAF ERCASKDHLW NNVRRGTRPE RLPVFDEECW QLMEACWDGD PLKRPLLGIV 910 920 QPMLRSIMDR LCKCSSEQPN RGLDDST
	Isoform 2. Checksum: 409A71F58BDBEC1B	FASTA	922	104,369
	10 20 30 40 50 60 MEADGQSWAG ESVSGPGPGG GGMIRELCRG FSRYRRYLGR LRQNLRETQK FFRDIKCSHS 70 80 90 100 110 120 HSCPSSPAGG GAAELGPAGD VAEAPLPAGQ LSCISFPPME ETYLQQLVDR LPCILILGQD 130 140 150 160 170 180 CNAKCQLLNL LLGVQVLPTL KLDSDESCKL RRLRFTYGTR TRVSLALPGQ YELVHTLASH 190 200 210 220 230 240 QDNWETIPEE DLEVQEDSED AAHVLADLEV TMHHALLQEV DIVVAPCPSH RPSVDVLSDL 250 260 270 280 290 300 ANDFLPVITY ALHKDELSER GEQELREVRQ YFSFPMFFFK VPKLEIISSS SGRAESERSP 310 320 330 340 350 360 LYGQLVDLGY LSSSHRNCVP SDQDCKAQSM LVEQSEKLKQ LSTFSHQLLQ NRLVDAAKAL 370 380 390 400 410 420 NVVHSHCLDI FINQAFDMQR DLQITPKRLE YTRKKENELY ESLMNIANRK QEEMKDMIVE 430 440 450 460 470 480 TLNTMKEELL DDAANMEFKD VIVPENGETI GTREIKSCIR QIQELIISRL NQAVANKLIS 490 500 510 520 530 540 SVDYLRESFV GTLERCLQSL EKSQDVSVHI TSNYLKQILN AAYHVEVTFH SGSSVTRMLW 550 560 570 580 590 600 EQIKQIIQRI TWVNPPTITL EWKRKVAQEA IDSLSASKLA KSICSQFRTR LNSSHEAFAA 610 620 630 640 650 660 SLRQLEAGHS GRLEKTEDLW LKVRKDHAPR LARLSLESRS LQDVLLHRKP KLGQELGRGQ 670 680 690 700 710 720 YGVVYLCDNW GGHFPCALKS VVPPDEKHWN DLALEFHYMR SLPKHERLVD LHGSVIDYNY 730 740 750 760 770 780 GGGSSVAVLL IMERLHRDLY TGLKAGLTLE TRLQIALDVV EGIRFLHSQG LVHRDIKLKN 790 800 810 820 830 840 VLLDKQNRAK ITDLGFCKPE AMMSGSIVGT PIHMAPELFT GKYDNSVDVY AFGILFWYIC 850 860 870 880 890 900 SGSIKLPEAF ERCASKDHLW NNVRRGTRPE RLPVFDEECW QLMEACWDGD PLKRPLLGIV 910 920 QPMLRSIMDR LCKCSSEQPN RG
	Isoform 3. Checksum: 6A1476C7A9A9E505	FASTA	918	103,870
	10 20 30 40 50 60 MEADGQSWAG ESVSGPGPGG GGMIRELCRG FSRYRRYLGR LRQNLRETQK FFRDIKCSHS 70 80 90 100 110 120 HSCPSSPAGG GAAELGPAGD VAEAPLPAGQ LSCISFPPME ETYLQQLVDR LPCILILGQD 130 140 150 160 170 180 CNAKCQLLNL LLGVQVLPTL KLDSDESCKL RRLRFTYGTR TRVSLALPGQ YELVHTLASH 190 200 210 220 230 240 QDNWETIPEE DLEVQEDSED AAHVLADLEE VDIVVAPCPS HRPSVDVLSD LANDFLPVIT 250 260 270 280 290 300 YALHKDELSE RGEQELREVR QYFSFPMFFF KVPKLEIISS SSGRAESERS PLYGQLVDLG 310 320 330 340 350 360 YLSSSHRNCV PSDQDCKAQS MLVEQSEKLK QLSTFSHQLL QNRLVDAAKA LNVVHSHCLD 370 380 390 400 410 420 IFINQAFDMQ RDLQITPKRL EYTRKKENEL YESLMNIANR KQEEMKDMIV ETLNTMKEEL 430 440 450 460 470 480 LDDAANMEFK DVIVPENGET IGTREIKSCI RQIQELIISR LNQAVANKLI SSVDYLRESF 490 500 510 520 530 540 VGTLERCLQS LEKSQDVSVH ITSNYLKQIL NAAYHVEVTF HSGSSVTRML WEQIKQIIQR 550 560 570 580 590 600 ITWVNPPTIT LEWKRKVAQE AIDSLSASKL AKSICSQFRT RLNSSHEAFA ASLRQLEAGH 610 620 630 640 650 660 SGRLEKTEDL WLKVRKDHAP RLARLSLESR SLQDVLLHRK PKLGQELGRG QYGVVYLCDN 670 680 690 700 710 720 WGGHFPCALK SVVPPDEKHW NDLALEFHYM RSLPKHERLV DLHGSVIDYN YGGGSSVAVL 730 740 750 760 770 780 LIMERLHRDL YTGLKAGLTL ETRLQIALDV VEGIRFLHSQ GLVHRDIKLK NVLLDKQNRA 790 800 810 820 830 840 KITDLGFCKP EAMMSGSIVG TPIHMAPELF TGKYDNSVDV YAFGILFWYI CSGSIKLPEA 850 860 870 880 890 900 FERCASKDHL WNNVRRGTRP ERLPVFDEEC WQLMEACWDG DPLKRPLLGI VQPMLRSIMD 910 RLCKCSSEQP NRGLDDST
	Isoform 4. Checksum: 20814A1ABE0AC58A	FASTA	164	18,814
	10 20 30 40 50 60 MISLSSSWQI IQRITWVNPP TITLEWKRKV AQEAIDSLSA SKLAKSICSQ FRTRLNSSHE 70 80 90 100 110 120 AFAASLRQLE AGHSGRLEKT EDLWLKVRKD HAPRLARLSL ESRSLQDVLL HRKPKLGQEL 130 140 150 160 GRGQYGVVYL CDNWGGHFPC ALKSVVPPDE KHWNDLALEF HYMR

References

[1]	"Dusty protein kinases: primary structure, gene evolution, tissue specific expression and unique features of the catalytic domain." Peng J., Dong W., Chen Y., Mo R., Cheng J.-F., Hui C.-C., Mohandas N., Huang C.-H. Biochim. Biophys. Acta 1759:562-572(2006) [PubMed: 17123648] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: BALB/c. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-392 (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 535-927 (ISOFORM 1). Strain: C57BL/6J. Tissue: Cerebellum, Pancreas and Retina.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 478-927 (ISOFORM 1). Strain: C57BL/6. Tissue: Eye.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY208852 mRNA. Translation: AAP42420.1. AY429676 mRNA. Translation: AAS55392.1. AK020880 mRNA. Translation: BAB32238.1. AK043199 mRNA. Translation: BAC31487.1. AK050542 mRNA. Translation: BAC34316.1. Different initiation. AK145449 mRNA. Translation: BAE26444.1. Different initiation. BC089380 mRNA. Translation: AAH89380.1.
IPI	IPI00399993. IPI00754249. IPI00757155. IPI00758158.
RefSeq	NP_766104.2.
UniGene	Mm.257120 Mm.421296
3D structure databases
HSSP	HSSP built from PDB template 1VR2 based on UniProtKB P35968.
ModBase	Search...
PTM databases
PhosphoSite	Q6XUX1.
Proteomic databases
PRIDE	Q6XUX1.
Genome annotation databases
Ensembl	ENSMUST00000045110; ENSMUSP00000035358; ENSMUSG00000042046; Mus musculus. [Genome view]
GeneID	213452.
KEGG	mmu:213452.
UCSC	uc007cou.1. mouse. uc007cov.1. mouse.
Organism-specific databases
CTD	213452.
MGI	MGI:1925064. Dstyk.
Phylogenomic databases
HOGENOM	Q6XUX1.
HOVERGEN	Q6XUX1.
OMA	DFLPVIT.
Enzyme and pathway databases
BRENDA	2.7.11.1. 244.
Gene expression databases
ArrayExpress	Q6XUX1.
Bgee	Q6XUX1.
CleanEx	MM_RIPK5.
Genevestigator	Q6XUX1.
GermOnline	ENSMUSG00000042046. Mus musculus.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	373980.
SOURCE	Search...

Entry information

Entry name

DUSTY_MOUSE

Accession

Primary (citable) accession number: Q6XUX1
Secondary accession number(s): Q3ULK4 , Q5EBN5, Q8C7D4, Q8C923, Q9CTP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)