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Q6XQN6

- PNCB_HUMAN

UniProt

Q6XQN6 - PNCB_HUMAN

Protein

Nicotinate phosphoribosyltransferase

Gene

NAPRT

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 2 (15 Jan 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN). Essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells.1 Publication
    Catalyzes the synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP.By similarity

    Catalytic activityi

    Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate.

    Kineticsi

    1. KM=44.3 µM for nicotinic acid (in the presence of 3mM ATP)1 Publication
    2. KM=22.1 µM for 5-phosphoribosyl-1-pyrophosphate (in the presence of 3mM ATP)1 Publication
    3. KM=27.3 µM for nicotinic acid (in the presence of inorganic phosphate)1 Publication
    4. KM=38.2 µM for 5-phosphoribosyl-1-pyrophosphate (in the presence of inorganic phosphate)1 Publication

    Pathwayi

    GO - Molecular functioni

    1. ligase activity Source: UniProtKB-KW
    2. nicotinate-nucleotide diphosphorylase (carboxylating) activity Source: InterPro
    3. nicotinate phosphoribosyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. NAD biosynthetic process Source: UniProtKB-UniPathway
    2. NAD metabolic process Source: Reactome
    3. nicotinamide metabolic process Source: Reactome
    4. nicotinate nucleotide salvage Source: InterPro
    5. response to oxidative stress Source: UniProtKB
    6. small molecule metabolic process Source: Reactome
    7. vitamin metabolic process Source: Reactome
    8. water-soluble vitamin metabolic process Source: Reactome

    Keywords - Molecular functioni

    Ligase, Transferase

    Keywords - Biological processi

    Pyridine nucleotide biosynthesis

    Enzyme and pathway databases

    BRENDAi2.4.2.11. 2681.
    ReactomeiREACT_11213. Nicotinamide salvaging.
    UniPathwayiUPA00253; UER00457.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nicotinate phosphoribosyltransferase (EC:6.3.4.21)
    Short name:
    NAPRTase
    Alternative name(s):
    FHA-HIT-interacting protein
    Nicotinate phosphoribosyltransferase domain-containing protein 1
    Gene namesi
    Name:NAPRT
    Synonyms:FHIP, NAPRT1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:30450. NAPRT.

    Subcellular locationi

    Cytoplasmcytosol 1 Publication

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. extracellular vesicular exosome Source: UniProt

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi19 – 191D → A: Complete loss of activity. 1 Publication
    Mutagenesisi21 – 211Y → A: Partial loss of activity in the presence of ATP, complete loss in the absence of ATP. 1 Publication
    Mutagenesisi169 – 1691G → A: Partial loss of activity. 1 Publication
    Mutagenesisi209 – 2091G → A: Partial loss of activity. 1 Publication
    Mutagenesisi213 – 2131H → A: Partial loss of activity. 1 Publication
    Mutagenesisi288 – 2881D → A: Partial loss of activity. 1 Publication
    Mutagenesisi318 – 3181R → A: Partial loss of activity in the presence of ATP, almost complete loss in the absence of ATP. 1 Publication
    Mutagenesisi357 – 3571N → A: Small loss of activity. 1 Publication
    Mutagenesisi379 – 3791G → A: Complete loss of activity. 1 Publication
    Mutagenesisi380 – 3801T → A: Partial loss of activity. 1 Publication
    Mutagenesisi381 – 3811S → A: Partial loss of activity. 1 Publication

    Organism-specific databases

    PharmGKBiPA142671293.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 538538Nicotinate phosphoribosyltransferasePRO_0000315681Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei537 – 5371Phosphoserine5 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ6XQN6.
    PaxDbiQ6XQN6.
    PRIDEiQ6XQN6.

    PTM databases

    PhosphoSiteiQ6XQN6.

    Expressioni

    Gene expression databases

    ArrayExpressiQ6XQN6.
    BgeeiQ6XQN6.
    CleanExiHS_NAPRT1.
    GenevestigatoriQ6XQN6.

    Organism-specific databases

    HPAiHPA023739.
    HPA024017.

    Interactioni

    Protein-protein interaction databases

    BioGridi125001. 9 interactions.
    STRINGi9606.ENSP00000401508.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6XQN6.
    SMRiQ6XQN6. Positions 16-522.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NAPRTase family.Curated

    Phylogenomic databases

    eggNOGiCOG1488.
    HOVERGENiHBG062439.
    InParanoidiQ6XQN6.
    KOiK00763.
    PhylomeDBiQ6XQN6.
    TreeFamiTF314732.

    Family and domain databases

    InterProiIPR007229. Nic_PRibTrfase-Fam.
    IPR006405. Nic_PRibTrfase_pncB.
    IPR002638. Quinolinate_PRibosylTrfase_C.
    [Graphical view]
    PANTHERiPTHR11098. PTHR11098. 1 hit.
    PfamiPF04095. NAPRTase. 2 hits.
    [Graphical view]
    PIRSFiPIRSF000484. NAPRT. 1 hit.
    SUPFAMiSSF51690. SSF51690. 2 hits.
    TIGRFAMsiTIGR01513. NAPRTase_put. 1 hit.

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6XQN6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAEQDPEAR AAARPLLTDL YQATMALGYW RAGRARDAAE FELFFRRCPF    50
    GGAFALAAGL RDCVRFLRAF RLRDADVQFL ASVLPPDTDP AFFEHLRALD 100
    CSEVTVRALP EGSLAFPGVP LLQVSGPLLV VQLLETPLLC LVSYASLVAT 150
    NAARLRLIAG PEKRLLEMGL RRAQGPDGGL TASTYSYLGG FDSSSNVLAG 200
    QLRGVPVAGT LAHSFVTSFS GSEVPPDPML APAAGEGPGV DLAAKAQVWL 250
    EQVCAHLGLG VQEPHPGERA AFVAYALAFP RAFQGLLDTY SVWRSGLPNF 300
    LAVALALGEL GYRAVGVRLD SGDLLQQAQE IRKVFRAAAA QFQVPWLESV 350
    LIVVSNNIDE EALARLAQEG SEVNVIGIGT SVVTCPQQPS LGGVYKLVAV 400
    GGQPRMKLTE DPEKQTLPGS KAAFRLLGSD GSPLMDMLQL AEEPVPQAGQ 450
    ELRVWPPGAQ EPCTVRPAQV EPLLRLCLQQ GQLCEPLPSL AESRALAQLS 500
    LSRLSPEHRR LRSPAQYQVV LSERLQALVN SLCAGQSP 538
    Length:538
    Mass (Da):57,578
    Last modified:January 15, 2008 - v2
    Checksum:i26DF39885CBB1C9B
    GO
    Isoform 2 (identifier: Q6XQN6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         517-517: Y → YQVGGGGPPCHSALCAPALTLPTAPVLCSL

    Note: No experimental confirmation available.

    Show »
    Length:567
    Mass (Da):60,291
    Checksum:i3FFE59FEFD270DF9
    GO
    Isoform 3 (identifier: Q6XQN6-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         469-481: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:525
    Mass (Da):56,099
    Checksum:iE18F7E02CB00ABF4
    GO

    Sequence cautioni

    The sequence AAH06284.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence AAH32466.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti155 – 1551L → V in AAP69603. 1 PublicationCurated
    Sequence conflicti155 – 1551L → V in AAP69604. 1 PublicationCurated
    Sequence conflicti155 – 1551L → V in AAP69605. 1 PublicationCurated
    Sequence conflicti224 – 2241V → A in BAF75377. (PubMed:17604275)Curated
    Sequence conflicti246 – 2461A → T in BAF75377. (PubMed:17604275)Curated
    Sequence conflicti375 – 3751V → A in BAF75377. (PubMed:17604275)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti57 – 571A → V.
    Corresponds to variant rs896950 [ dbSNP | Ensembl ].
    VAR_038275

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei469 – 48113Missing in isoform 3. 1 PublicationVSP_030610Add
    BLAST
    Alternative sequencei517 – 5171Y → YQVGGGGPPCHSALCAPALT LPTAPVLCSL in isoform 2. 1 PublicationVSP_030612

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB242230 mRNA. Translation: BAF75377.1.
    AY214325 mRNA. Translation: AAP69603.1.
    AY214326 mRNA. Translation: AAP69604.1.
    AY214327 mRNA. Translation: AAP69605.1.
    BC006284 mRNA. Translation: AAH06284.2. Different initiation.
    BC032466 mRNA. Translation: AAH32466.1. Different initiation.
    CCDSiCCDS6403.2. [Q6XQN6-1]
    CCDS69555.1. [Q6XQN6-3]
    RefSeqiNP_001273758.1. NM_001286829.1. [Q6XQN6-3]
    NP_660202.3. NM_145201.5. [Q6XQN6-1]
    UniGeneiHs.493164.

    Genome annotation databases

    EnsembliENST00000426292; ENSP00000390949; ENSG00000147813. [Q6XQN6-3]
    ENST00000449291; ENSP00000401508; ENSG00000147813. [Q6XQN6-1]
    GeneIDi93100.
    KEGGihsa:93100.
    UCSCiuc003yym.4. human. [Q6XQN6-1]
    uc003yyn.4. human. [Q6XQN6-3]

    Polymorphism databases

    DMDMi166221824.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB242230 mRNA. Translation: BAF75377.1 .
    AY214325 mRNA. Translation: AAP69603.1 .
    AY214326 mRNA. Translation: AAP69604.1 .
    AY214327 mRNA. Translation: AAP69605.1 .
    BC006284 mRNA. Translation: AAH06284.2 . Different initiation.
    BC032466 mRNA. Translation: AAH32466.1 . Different initiation.
    CCDSi CCDS6403.2. [Q6XQN6-1 ]
    CCDS69555.1. [Q6XQN6-3 ]
    RefSeqi NP_001273758.1. NM_001286829.1. [Q6XQN6-3 ]
    NP_660202.3. NM_145201.5. [Q6XQN6-1 ]
    UniGenei Hs.493164.

    3D structure databases

    ProteinModelPortali Q6XQN6.
    SMRi Q6XQN6. Positions 16-522.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 125001. 9 interactions.
    STRINGi 9606.ENSP00000401508.

    PTM databases

    PhosphoSitei Q6XQN6.

    Polymorphism databases

    DMDMi 166221824.

    Proteomic databases

    MaxQBi Q6XQN6.
    PaxDbi Q6XQN6.
    PRIDEi Q6XQN6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000426292 ; ENSP00000390949 ; ENSG00000147813 . [Q6XQN6-3 ]
    ENST00000449291 ; ENSP00000401508 ; ENSG00000147813 . [Q6XQN6-1 ]
    GeneIDi 93100.
    KEGGi hsa:93100.
    UCSCi uc003yym.4. human. [Q6XQN6-1 ]
    uc003yyn.4. human. [Q6XQN6-3 ]

    Organism-specific databases

    CTDi 93100.
    GeneCardsi GC08M144656.
    HGNCi HGNC:30450. NAPRT.
    HPAi HPA023739.
    HPA024017.
    MIMi 611552. gene.
    neXtProti NX_Q6XQN6.
    PharmGKBi PA142671293.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1488.
    HOVERGENi HBG062439.
    InParanoidi Q6XQN6.
    KOi K00763.
    PhylomeDBi Q6XQN6.
    TreeFami TF314732.

    Enzyme and pathway databases

    UniPathwayi UPA00253 ; UER00457 .
    BRENDAi 2.4.2.11. 2681.
    Reactomei REACT_11213. Nicotinamide salvaging.

    Miscellaneous databases

    ChiTaRSi NAPRT1. human.
    GenomeRNAii 93100.
    NextBioi 77980.
    PROi Q6XQN6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q6XQN6.
    Bgeei Q6XQN6.
    CleanExi HS_NAPRT1.
    Genevestigatori Q6XQN6.

    Family and domain databases

    InterProi IPR007229. Nic_PRibTrfase-Fam.
    IPR006405. Nic_PRibTrfase_pncB.
    IPR002638. Quinolinate_PRibosylTrfase_C.
    [Graphical view ]
    PANTHERi PTHR11098. PTHR11098. 1 hit.
    Pfami PF04095. NAPRTase. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF000484. NAPRT. 1 hit.
    SUPFAMi SSF51690. SSF51690. 2 hits.
    TIGRFAMsi TIGR01513. NAPRTase_put. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Elevation of cellular NAD levels by nicotinic acid and involvement of nicotinic acid phosphoribosyltransferase in human cells."
      Hara N., Yamada K., Shibata T., Osago H., Hashimoto T., Tsuchiya M.
      J. Biol. Chem. 282:24574-24582(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION.
    2. "Identification of nicotinate phosphoribosyltransferase as a novel FHA-HIT interaction protein (FHIP)."
      Huang C.-H., Chen H., Chen Y.
      Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Placenta.
    4. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-537, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    5. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
      Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
      Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-537, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-537, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-537, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-537, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Characterization of human nicotinate phosphoribosyltransferase: Kinetic studies, structure prediction and functional analysis by site-directed mutagenesis."
      Galassi L., Di Stefano M., Brunetti L., Orsomando G., Amici A., Ruggieri S., Magni G.
      Biochimie 94:300-309(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, CHARACTERIZATION, 3D-STRUCTURE MODELING, MUTAGENESIS OF ASP-19; TYR-21; GLY-169; GLY-209; HIS-213; ASP-288; ARG-318; ASN-357; GLY-379; THR-380 AND SER-381.

    Entry informationi

    Entry nameiPNCB_HUMAN
    AccessioniPrimary (citable) accession number: Q6XQN6
    Secondary accession number(s): A7BFI3
    , Q6PJL1, Q6XQN4, Q6XQN5, Q8N5E8, Q9BRG0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: January 15, 2008
    Last modified: October 1, 2014
    This is version 86 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3