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Protein

Nicotinate phosphoribosyltransferase

Gene

NAPRT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN). Essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells.1 Publication
Catalyzes the synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP.By similarity

Catalytic activityi

Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate.

Kineticsi

  1. KM=44.3 µM for nicotinic acid (in the presence of 3 mM ATP)1 Publication
  2. KM=22.1 µM for 5-phosphoribosyl-1-pyrophosphate (in the presence of 3 mM ATP)1 Publication
  3. KM=27.3 µM for nicotinic acid (in the presence of inorganic phosphate)1 Publication
  4. KM=38.2 µM for 5-phosphoribosyl-1-pyrophosphate (in the presence of inorganic phosphate)1 Publication

    Pathwayi: NAD(+) biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes nicotinate D-ribonucleotide from nicotinate.
    Proteins known to be involved in this subpathway in this organism are:
    1. Nicotinate phosphoribosyltransferase (NAPRT)
    This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes nicotinate D-ribonucleotide from nicotinate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

    GO - Molecular functioni

    GO - Biological processi

    • NAD biosynthetic process Source: UniProtKB-UniPathway
    • nicotinamide metabolic process Source: Reactome
    • nicotinate nucleotide salvage Source: InterPro
    • response to oxidative stress Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Ligase, Transferase

    Keywords - Biological processi

    Pyridine nucleotide biosynthesis

    Enzyme and pathway databases

    BioCyciZFISH:HS14222-MONOMER.
    BRENDAi6.3.4.21. 2681.
    ReactomeiR-HSA-197264. Nicotinamide salvaging.
    R-HSA-6798695. Neutrophil degranulation.
    UniPathwayiUPA00253; UER00457.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nicotinate phosphoribosyltransferase (EC:6.3.4.21)
    Short name:
    NAPRTase
    Alternative name(s):
    FHA-HIT-interacting protein
    Nicotinate phosphoribosyltransferase domain-containing protein 1
    Gene namesi
    Name:NAPRT
    Synonyms:FHIP, NAPRT1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:30450. NAPRT.

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: HPA
    • cytosol Source: UniProtKB
    • extracellular exosome Source: UniProtKB
    • Golgi apparatus Source: HPA
    • nucleus Source: HPA
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi19D → A: Complete loss of activity. 1 Publication1
    Mutagenesisi21Y → A: Partial loss of activity in the presence of ATP, complete loss in the absence of ATP. 1 Publication1
    Mutagenesisi169G → A: Partial loss of activity. 1 Publication1
    Mutagenesisi209G → A: Partial loss of activity. 1 Publication1
    Mutagenesisi213H → A: Partial loss of activity. 1 Publication1
    Mutagenesisi288D → A: Partial loss of activity. 1 Publication1
    Mutagenesisi318R → A: Partial loss of activity in the presence of ATP, almost complete loss in the absence of ATP. 1 Publication1
    Mutagenesisi357N → A: Small loss of activity. 1 Publication1
    Mutagenesisi379G → A: Complete loss of activity. 1 Publication1
    Mutagenesisi380T → A: Partial loss of activity. 1 Publication1
    Mutagenesisi381S → A: Partial loss of activity. 1 Publication1

    Organism-specific databases

    DisGeNETi93100.
    OpenTargetsiENSG00000147813.
    ENSG00000278488.
    PharmGKBiPA142671293.

    Polymorphism and mutation databases

    DMDMi166221824.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003156811 – 538Nicotinate phosphoribosyltransferaseAdd BLAST538

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei537PhosphoserineCombined sources1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    EPDiQ6XQN6.
    MaxQBiQ6XQN6.
    PaxDbiQ6XQN6.
    PeptideAtlasiQ6XQN6.
    PRIDEiQ6XQN6.

    PTM databases

    iPTMnetiQ6XQN6.
    PhosphoSitePlusiQ6XQN6.

    Expressioni

    Gene expression databases

    BgeeiENSG00000147813.
    CleanExiHS_NAPRT1.
    ExpressionAtlasiQ6XQN6. baseline and differential.
    GenevisibleiQ6XQN6. HS.

    Organism-specific databases

    HPAiCAB068206.
    CAB068207.
    HPA023739.
    HPA024017.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CCDC57Q2TAC23EBI-10254820,EBI-2808286
    EHMT2A2ABF93EBI-10254820,EBI-10174566
    GRAMD3Q96HH93EBI-10254872,EBI-2832937
    KRT40Q6A1623EBI-10254820,EBI-10171697
    RBPMSQ930623EBI-10254820,EBI-740322

    Protein-protein interaction databases

    BioGridi125001. 35 interactors.
    IntActiQ6XQN6. 6 interactors.
    STRINGi9606.ENSP00000401508.

    Structurei

    Secondary structure

    1538
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi20 – 31Combined sources12
    Beta strandi35 – 44Combined sources10
    Helixi49 – 51Combined sources3
    Helixi60 – 66Combined sources7
    Helixi74 – 79Combined sources6
    Helixi89 – 91Combined sources3
    Helixi96 – 98Combined sources3
    Beta strandi105 – 108Combined sources4
    Beta strandi121 – 127Combined sources7
    Helixi128 – 133Combined sources6
    Helixi135 – 159Combined sources25
    Beta strandi166 – 168Combined sources3
    Helixi178 – 189Combined sources12
    Beta strandi192 – 195Combined sources4
    Helixi197 – 203Combined sources7
    Helixi213 – 216Combined sources4
    Beta strandi233 – 237Combined sources5
    Helixi242 – 257Combined sources16
    Helixi266 – 278Combined sources13
    Helixi280 – 282Combined sources3
    Beta strandi283 – 286Combined sources4
    Turni292 – 295Combined sources4
    Helixi296 – 306Combined sources11
    Turni307 – 310Combined sources4
    Beta strandi314 – 319Combined sources6
    Helixi324 – 342Combined sources19
    Helixi345 – 348Combined sources4
    Beta strandi351 – 354Combined sources4
    Helixi360 – 366Combined sources7
    Beta strandi375 – 377Combined sources3
    Beta strandi394 – 400Combined sources7
    Beta strandi421 – 427Combined sources7
    Beta strandi429 – 431Combined sources3
    Beta strandi433 – 440Combined sources8
    Beta strandi451 – 454Combined sources4
    Beta strandi463 – 465Combined sources3
    Beta strandi468 – 472Combined sources5
    Beta strandi475 – 479Combined sources5
    Helixi490 – 503Combined sources14
    Helixi506 – 509Combined sources4
    Beta strandi511 – 513Combined sources3
    Beta strandi518 – 522Combined sources5
    Helixi523 – 529Combined sources7

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4YUBX-ray2.90A/B1-538[»]
    ProteinModelPortaliQ6XQN6.
    SMRiQ6XQN6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NAPRTase family.Curated

    Phylogenomic databases

    eggNOGiKOG2511. Eukaryota.
    COG1488. LUCA.
    GeneTreeiENSGT00390000002664.
    HOVERGENiHBG062439.
    InParanoidiQ6XQN6.
    KOiK00763.
    OMAiHSYVEAF.
    OrthoDBiEOG091G088G.
    PhylomeDBiQ6XQN6.
    TreeFamiTF314732.

    Family and domain databases

    InterProiIPR007229. Nic_PRibTrfase-Fam.
    IPR006405. Nic_PRibTrfase_pncB.
    IPR002638. Quinolinate_PRibosylTrfase_C.
    [Graphical view]
    PANTHERiPTHR11098. PTHR11098. 1 hit.
    PIRSFiPIRSF000484. NAPRT. 1 hit.
    SUPFAMiSSF51690. SSF51690. 2 hits.
    TIGRFAMsiTIGR01513. NAPRTase_put. 1 hit.

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q6XQN6-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MAAEQDPEAR AAARPLLTDL YQATMALGYW RAGRARDAAE FELFFRRCPF
    60 70 80 90 100
    GGAFALAAGL RDCVRFLRAF RLRDADVQFL ASVLPPDTDP AFFEHLRALD
    110 120 130 140 150
    CSEVTVRALP EGSLAFPGVP LLQVSGPLLV VQLLETPLLC LVSYASLVAT
    160 170 180 190 200
    NAARLRLIAG PEKRLLEMGL RRAQGPDGGL TASTYSYLGG FDSSSNVLAG
    210 220 230 240 250
    QLRGVPVAGT LAHSFVTSFS GSEVPPDPML APAAGEGPGV DLAAKAQVWL
    260 270 280 290 300
    EQVCAHLGLG VQEPHPGERA AFVAYALAFP RAFQGLLDTY SVWRSGLPNF
    310 320 330 340 350
    LAVALALGEL GYRAVGVRLD SGDLLQQAQE IRKVFRAAAA QFQVPWLESV
    360 370 380 390 400
    LIVVSNNIDE EALARLAQEG SEVNVIGIGT SVVTCPQQPS LGGVYKLVAV
    410 420 430 440 450
    GGQPRMKLTE DPEKQTLPGS KAAFRLLGSD GSPLMDMLQL AEEPVPQAGQ
    460 470 480 490 500
    ELRVWPPGAQ EPCTVRPAQV EPLLRLCLQQ GQLCEPLPSL AESRALAQLS
    510 520 530
    LSRLSPEHRR LRSPAQYQVV LSERLQALVN SLCAGQSP
    Length:538
    Mass (Da):57,578
    Last modified:January 15, 2008 - v2
    Checksum:i26DF39885CBB1C9B
    GO
    Isoform 2 (identifier: Q6XQN6-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         517-517: Y → YQVGGGGPPCHSALCAPALTLPTAPVLCSL

    Note: No experimental confirmation available.
    Show »
    Length:567
    Mass (Da):60,291
    Checksum:i3FFE59FEFD270DF9
    GO
    Isoform 3 (identifier: Q6XQN6-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         469-481: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:525
    Mass (Da):56,099
    Checksum:iE18F7E02CB00ABF4
    GO

    Sequence cautioni

    The sequence AAH06284 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
    The sequence AAH32466 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti155L → V in AAP69603 (Ref. 2) Curated1
    Sequence conflicti155L → V in AAP69604 (Ref. 2) Curated1
    Sequence conflicti155L → V in AAP69605 (Ref. 2) Curated1
    Sequence conflicti224V → A in BAF75377 (PubMed:17604275).Curated1
    Sequence conflicti246A → T in BAF75377 (PubMed:17604275).Curated1
    Sequence conflicti375V → A in BAF75377 (PubMed:17604275).Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_03827557A → V.Corresponds to variant rs896950dbSNPEnsembl.1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_030610469 – 481Missing in isoform 3. 1 PublicationAdd BLAST13
    Alternative sequenceiVSP_030612517Y → YQVGGGGPPCHSALCAPALT LPTAPVLCSL in isoform 2. 1 Publication1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB242230 mRNA. Translation: BAF75377.1.
    AY214325 mRNA. Translation: AAP69603.1.
    AY214326 mRNA. Translation: AAP69604.1.
    AY214327 mRNA. Translation: AAP69605.1.
    BC006284 mRNA. Translation: AAH06284.2. Different initiation.
    BC032466 mRNA. Translation: AAH32466.1. Different initiation.
    CCDSiCCDS6403.2. [Q6XQN6-1]
    CCDS69555.1. [Q6XQN6-3]
    RefSeqiNP_001273758.1. NM_001286829.1. [Q6XQN6-3]
    NP_660202.3. NM_145201.5. [Q6XQN6-1]
    XP_016869469.1. XM_017013980.1.
    UniGeneiHs.493164.

    Genome annotation databases

    EnsembliENST00000426292; ENSP00000390949; ENSG00000147813. [Q6XQN6-3]
    ENST00000449291; ENSP00000401508; ENSG00000147813. [Q6XQN6-1]
    ENST00000621955; ENSP00000480017; ENSG00000278488. [Q6XQN6-1]
    ENST00000632139; ENSP00000488794; ENSG00000278488. [Q6XQN6-3]
    GeneIDi93100.
    KEGGihsa:93100.
    UCSCiuc003yym.6. human. [Q6XQN6-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB242230 mRNA. Translation: BAF75377.1.
    AY214325 mRNA. Translation: AAP69603.1.
    AY214326 mRNA. Translation: AAP69604.1.
    AY214327 mRNA. Translation: AAP69605.1.
    BC006284 mRNA. Translation: AAH06284.2. Different initiation.
    BC032466 mRNA. Translation: AAH32466.1. Different initiation.
    CCDSiCCDS6403.2. [Q6XQN6-1]
    CCDS69555.1. [Q6XQN6-3]
    RefSeqiNP_001273758.1. NM_001286829.1. [Q6XQN6-3]
    NP_660202.3. NM_145201.5. [Q6XQN6-1]
    XP_016869469.1. XM_017013980.1.
    UniGeneiHs.493164.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4YUBX-ray2.90A/B1-538[»]
    ProteinModelPortaliQ6XQN6.
    SMRiQ6XQN6.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi125001. 35 interactors.
    IntActiQ6XQN6. 6 interactors.
    STRINGi9606.ENSP00000401508.

    PTM databases

    iPTMnetiQ6XQN6.
    PhosphoSitePlusiQ6XQN6.

    Polymorphism and mutation databases

    DMDMi166221824.

    Proteomic databases

    EPDiQ6XQN6.
    MaxQBiQ6XQN6.
    PaxDbiQ6XQN6.
    PeptideAtlasiQ6XQN6.
    PRIDEiQ6XQN6.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000426292; ENSP00000390949; ENSG00000147813. [Q6XQN6-3]
    ENST00000449291; ENSP00000401508; ENSG00000147813. [Q6XQN6-1]
    ENST00000621955; ENSP00000480017; ENSG00000278488. [Q6XQN6-1]
    ENST00000632139; ENSP00000488794; ENSG00000278488. [Q6XQN6-3]
    GeneIDi93100.
    KEGGihsa:93100.
    UCSCiuc003yym.6. human. [Q6XQN6-1]

    Organism-specific databases

    CTDi93100.
    DisGeNETi93100.
    GeneCardsiNAPRT.
    HGNCiHGNC:30450. NAPRT.
    HPAiCAB068206.
    CAB068207.
    HPA023739.
    HPA024017.
    MIMi611552. gene.
    neXtProtiNX_Q6XQN6.
    OpenTargetsiENSG00000147813.
    ENSG00000278488.
    PharmGKBiPA142671293.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG2511. Eukaryota.
    COG1488. LUCA.
    GeneTreeiENSGT00390000002664.
    HOVERGENiHBG062439.
    InParanoidiQ6XQN6.
    KOiK00763.
    OMAiHSYVEAF.
    OrthoDBiEOG091G088G.
    PhylomeDBiQ6XQN6.
    TreeFamiTF314732.

    Enzyme and pathway databases

    UniPathwayiUPA00253; UER00457.
    BioCyciZFISH:HS14222-MONOMER.
    BRENDAi6.3.4.21. 2681.
    ReactomeiR-HSA-197264. Nicotinamide salvaging.
    R-HSA-6798695. Neutrophil degranulation.

    Miscellaneous databases

    ChiTaRSiFAM160A2. human.
    GenomeRNAii93100.
    PROiQ6XQN6.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000147813.
    CleanExiHS_NAPRT1.
    ExpressionAtlasiQ6XQN6. baseline and differential.
    GenevisibleiQ6XQN6. HS.

    Family and domain databases

    InterProiIPR007229. Nic_PRibTrfase-Fam.
    IPR006405. Nic_PRibTrfase_pncB.
    IPR002638. Quinolinate_PRibosylTrfase_C.
    [Graphical view]
    PANTHERiPTHR11098. PTHR11098. 1 hit.
    PIRSFiPIRSF000484. NAPRT. 1 hit.
    SUPFAMiSSF51690. SSF51690. 2 hits.
    TIGRFAMsiTIGR01513. NAPRTase_put. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPNCB_HUMAN
    AccessioniPrimary (citable) accession number: Q6XQN6
    Secondary accession number(s): A7BFI3
    , Q6PJL1, Q6XQN4, Q6XQN5, Q8N5E8, Q9BRG0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: January 15, 2008
    Last modified: November 30, 2016
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    7. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.