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Q6XQN6

- PNCB_HUMAN

UniProt

Q6XQN6 - PNCB_HUMAN

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Protein

Nicotinate phosphoribosyltransferase

Gene

NAPRT

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN). Essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells.1 Publication
Catalyzes the synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP.By similarity

Catalytic activityi

Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate.

Kineticsi

  1. KM=44.3 µM for nicotinic acid (in the presence of 3mM ATP)1 Publication
  2. KM=22.1 µM for 5-phosphoribosyl-1-pyrophosphate (in the presence of 3mM ATP)1 Publication
  3. KM=27.3 µM for nicotinic acid (in the presence of inorganic phosphate)1 Publication
  4. KM=38.2 µM for 5-phosphoribosyl-1-pyrophosphate (in the presence of inorganic phosphate)1 Publication

Pathwayi

GO - Molecular functioni

  1. ligase activity Source: UniProtKB-KW
  2. nicotinate-nucleotide diphosphorylase (carboxylating) activity Source: InterPro
  3. nicotinate phosphoribosyltransferase activity Source: UniProtKB

GO - Biological processi

  1. NAD biosynthetic process Source: UniProtKB-UniPathway
  2. NAD metabolic process Source: Reactome
  3. nicotinamide metabolic process Source: Reactome
  4. nicotinate nucleotide salvage Source: InterPro
  5. response to oxidative stress Source: UniProtKB
  6. small molecule metabolic process Source: Reactome
  7. vitamin metabolic process Source: Reactome
  8. water-soluble vitamin metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Ligase, Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Enzyme and pathway databases

BRENDAi2.4.2.11. 2681.
ReactomeiREACT_11213. Nicotinamide salvaging.
UniPathwayiUPA00253; UER00457.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate phosphoribosyltransferase (EC:6.3.4.21)
Short name:
NAPRTase
Alternative name(s):
FHA-HIT-interacting protein
Nicotinate phosphoribosyltransferase domain-containing protein 1
Gene namesi
Name:NAPRT
Synonyms:FHIP, NAPRT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 8

Organism-specific databases

HGNCiHGNC:30450. NAPRT.

Subcellular locationi

Cytoplasmcytosol 1 Publication

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. extracellular vesicular exosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi19 – 191D → A: Complete loss of activity. 1 Publication
Mutagenesisi21 – 211Y → A: Partial loss of activity in the presence of ATP, complete loss in the absence of ATP. 1 Publication
Mutagenesisi169 – 1691G → A: Partial loss of activity. 1 Publication
Mutagenesisi209 – 2091G → A: Partial loss of activity. 1 Publication
Mutagenesisi213 – 2131H → A: Partial loss of activity. 1 Publication
Mutagenesisi288 – 2881D → A: Partial loss of activity. 1 Publication
Mutagenesisi318 – 3181R → A: Partial loss of activity in the presence of ATP, almost complete loss in the absence of ATP. 1 Publication
Mutagenesisi357 – 3571N → A: Small loss of activity. 1 Publication
Mutagenesisi379 – 3791G → A: Complete loss of activity. 1 Publication
Mutagenesisi380 – 3801T → A: Partial loss of activity. 1 Publication
Mutagenesisi381 – 3811S → A: Partial loss of activity. 1 Publication

Organism-specific databases

PharmGKBiPA142671293.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 538538Nicotinate phosphoribosyltransferasePRO_0000315681Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei537 – 5371Phosphoserine5 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6XQN6.
PaxDbiQ6XQN6.
PRIDEiQ6XQN6.

PTM databases

PhosphoSiteiQ6XQN6.

Expressioni

Gene expression databases

BgeeiQ6XQN6.
CleanExiHS_NAPRT1.
ExpressionAtlasiQ6XQN6. baseline and differential.
GenevestigatoriQ6XQN6.

Organism-specific databases

HPAiHPA023739.
HPA024017.

Interactioni

Protein-protein interaction databases

BioGridi125001. 9 interactions.
STRINGi9606.ENSP00000401508.

Structurei

3D structure databases

ProteinModelPortaliQ6XQN6.
SMRiQ6XQN6. Positions 16-522.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAPRTase family.Curated

Phylogenomic databases

eggNOGiCOG1488.
GeneTreeiENSGT00390000002664.
HOVERGENiHBG062439.
InParanoidiQ6XQN6.
KOiK00763.
PhylomeDBiQ6XQN6.
TreeFamiTF314732.

Family and domain databases

InterProiIPR007229. Nic_PRibTrfase-Fam.
IPR006405. Nic_PRibTrfase_pncB.
IPR002638. Quinolinate_PRibosylTrfase_C.
[Graphical view]
PANTHERiPTHR11098. PTHR11098. 1 hit.
PfamiPF04095. NAPRTase. 2 hits.
[Graphical view]
PIRSFiPIRSF000484. NAPRT. 1 hit.
SUPFAMiSSF51690. SSF51690. 2 hits.
TIGRFAMsiTIGR01513. NAPRTase_put. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6XQN6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAEQDPEAR AAARPLLTDL YQATMALGYW RAGRARDAAE FELFFRRCPF
60 70 80 90 100
GGAFALAAGL RDCVRFLRAF RLRDADVQFL ASVLPPDTDP AFFEHLRALD
110 120 130 140 150
CSEVTVRALP EGSLAFPGVP LLQVSGPLLV VQLLETPLLC LVSYASLVAT
160 170 180 190 200
NAARLRLIAG PEKRLLEMGL RRAQGPDGGL TASTYSYLGG FDSSSNVLAG
210 220 230 240 250
QLRGVPVAGT LAHSFVTSFS GSEVPPDPML APAAGEGPGV DLAAKAQVWL
260 270 280 290 300
EQVCAHLGLG VQEPHPGERA AFVAYALAFP RAFQGLLDTY SVWRSGLPNF
310 320 330 340 350
LAVALALGEL GYRAVGVRLD SGDLLQQAQE IRKVFRAAAA QFQVPWLESV
360 370 380 390 400
LIVVSNNIDE EALARLAQEG SEVNVIGIGT SVVTCPQQPS LGGVYKLVAV
410 420 430 440 450
GGQPRMKLTE DPEKQTLPGS KAAFRLLGSD GSPLMDMLQL AEEPVPQAGQ
460 470 480 490 500
ELRVWPPGAQ EPCTVRPAQV EPLLRLCLQQ GQLCEPLPSL AESRALAQLS
510 520 530
LSRLSPEHRR LRSPAQYQVV LSERLQALVN SLCAGQSP
Length:538
Mass (Da):57,578
Last modified:January 15, 2008 - v2
Checksum:i26DF39885CBB1C9B
GO
Isoform 2 (identifier: Q6XQN6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     517-517: Y → YQVGGGGPPCHSALCAPALTLPTAPVLCSL

Note: No experimental confirmation available.

Show »
Length:567
Mass (Da):60,291
Checksum:i3FFE59FEFD270DF9
GO
Isoform 3 (identifier: Q6XQN6-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     469-481: Missing.

Note: No experimental confirmation available.

Show »
Length:525
Mass (Da):56,099
Checksum:iE18F7E02CB00ABF4
GO

Sequence cautioni

The sequence AAH06284.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence AAH32466.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti155 – 1551L → V in AAP69603. 1 PublicationCurated
Sequence conflicti155 – 1551L → V in AAP69604. 1 PublicationCurated
Sequence conflicti155 – 1551L → V in AAP69605. 1 PublicationCurated
Sequence conflicti224 – 2241V → A in BAF75377. (PubMed:17604275)Curated
Sequence conflicti246 – 2461A → T in BAF75377. (PubMed:17604275)Curated
Sequence conflicti375 – 3751V → A in BAF75377. (PubMed:17604275)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti57 – 571A → V.
Corresponds to variant rs896950 [ dbSNP | Ensembl ].
VAR_038275

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei469 – 48113Missing in isoform 3. 1 PublicationVSP_030610Add
BLAST
Alternative sequencei517 – 5171Y → YQVGGGGPPCHSALCAPALT LPTAPVLCSL in isoform 2. 1 PublicationVSP_030612

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB242230 mRNA. Translation: BAF75377.1.
AY214325 mRNA. Translation: AAP69603.1.
AY214326 mRNA. Translation: AAP69604.1.
AY214327 mRNA. Translation: AAP69605.1.
BC006284 mRNA. Translation: AAH06284.2. Different initiation.
BC032466 mRNA. Translation: AAH32466.1. Different initiation.
CCDSiCCDS6403.2. [Q6XQN6-1]
CCDS69555.1. [Q6XQN6-3]
RefSeqiNP_001273758.1. NM_001286829.1. [Q6XQN6-3]
NP_660202.3. NM_145201.5. [Q6XQN6-1]
UniGeneiHs.493164.

Genome annotation databases

EnsembliENST00000426292; ENSP00000390949; ENSG00000147813. [Q6XQN6-3]
ENST00000449291; ENSP00000401508; ENSG00000147813. [Q6XQN6-1]
GeneIDi93100.
KEGGihsa:93100.
UCSCiuc003yym.4. human. [Q6XQN6-1]
uc003yyn.4. human. [Q6XQN6-3]

Polymorphism databases

DMDMi166221824.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB242230 mRNA. Translation: BAF75377.1 .
AY214325 mRNA. Translation: AAP69603.1 .
AY214326 mRNA. Translation: AAP69604.1 .
AY214327 mRNA. Translation: AAP69605.1 .
BC006284 mRNA. Translation: AAH06284.2 . Different initiation.
BC032466 mRNA. Translation: AAH32466.1 . Different initiation.
CCDSi CCDS6403.2. [Q6XQN6-1 ]
CCDS69555.1. [Q6XQN6-3 ]
RefSeqi NP_001273758.1. NM_001286829.1. [Q6XQN6-3 ]
NP_660202.3. NM_145201.5. [Q6XQN6-1 ]
UniGenei Hs.493164.

3D structure databases

ProteinModelPortali Q6XQN6.
SMRi Q6XQN6. Positions 16-522.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 125001. 9 interactions.
STRINGi 9606.ENSP00000401508.

PTM databases

PhosphoSitei Q6XQN6.

Polymorphism databases

DMDMi 166221824.

Proteomic databases

MaxQBi Q6XQN6.
PaxDbi Q6XQN6.
PRIDEi Q6XQN6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000426292 ; ENSP00000390949 ; ENSG00000147813 . [Q6XQN6-3 ]
ENST00000449291 ; ENSP00000401508 ; ENSG00000147813 . [Q6XQN6-1 ]
GeneIDi 93100.
KEGGi hsa:93100.
UCSCi uc003yym.4. human. [Q6XQN6-1 ]
uc003yyn.4. human. [Q6XQN6-3 ]

Organism-specific databases

CTDi 93100.
GeneCardsi GC08M144656.
HGNCi HGNC:30450. NAPRT.
HPAi HPA023739.
HPA024017.
MIMi 611552. gene.
neXtProti NX_Q6XQN6.
PharmGKBi PA142671293.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1488.
GeneTreei ENSGT00390000002664.
HOVERGENi HBG062439.
InParanoidi Q6XQN6.
KOi K00763.
PhylomeDBi Q6XQN6.
TreeFami TF314732.

Enzyme and pathway databases

UniPathwayi UPA00253 ; UER00457 .
BRENDAi 2.4.2.11. 2681.
Reactomei REACT_11213. Nicotinamide salvaging.

Miscellaneous databases

ChiTaRSi NAPRT1. human.
GenomeRNAii 93100.
NextBioi 77980.
PROi Q6XQN6.
SOURCEi Search...

Gene expression databases

Bgeei Q6XQN6.
CleanExi HS_NAPRT1.
ExpressionAtlasi Q6XQN6. baseline and differential.
Genevestigatori Q6XQN6.

Family and domain databases

InterProi IPR007229. Nic_PRibTrfase-Fam.
IPR006405. Nic_PRibTrfase_pncB.
IPR002638. Quinolinate_PRibosylTrfase_C.
[Graphical view ]
PANTHERi PTHR11098. PTHR11098. 1 hit.
Pfami PF04095. NAPRTase. 2 hits.
[Graphical view ]
PIRSFi PIRSF000484. NAPRT. 1 hit.
SUPFAMi SSF51690. SSF51690. 2 hits.
TIGRFAMsi TIGR01513. NAPRTase_put. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Elevation of cellular NAD levels by nicotinic acid and involvement of nicotinic acid phosphoribosyltransferase in human cells."
    Hara N., Yamada K., Shibata T., Osago H., Hashimoto T., Tsuchiya M.
    J. Biol. Chem. 282:24574-24582(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION.
  2. "Identification of nicotinate phosphoribosyltransferase as a novel FHA-HIT interaction protein (FHIP)."
    Huang C.-H., Chen H., Chen Y.
    Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Placenta.
  4. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-537, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
    Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
    Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-537, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-537, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-537, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-537, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Characterization of human nicotinate phosphoribosyltransferase: Kinetic studies, structure prediction and functional analysis by site-directed mutagenesis."
    Galassi L., Di Stefano M., Brunetti L., Orsomando G., Amici A., Ruggieri S., Magni G.
    Biochimie 94:300-309(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, CHARACTERIZATION, 3D-STRUCTURE MODELING, MUTAGENESIS OF ASP-19; TYR-21; GLY-169; GLY-209; HIS-213; ASP-288; ARG-318; ASN-357; GLY-379; THR-380 AND SER-381.

Entry informationi

Entry nameiPNCB_HUMAN
AccessioniPrimary (citable) accession number: Q6XQN6
Secondary accession number(s): A7BFI3
, Q6PJL1, Q6XQN4, Q6XQN5, Q8N5E8, Q9BRG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: October 29, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3