Reviewed, UniProtKB/Swiss-Prot Q6XQI9 (L_ALLVP)

Names and origin

Protein names

Recommended name:
    RNA-directed RNA polymerase
    EC=2.7.7.48
Alternative name(s):
    Protein L

Gene names

Name:

L

Organism

Allpahuayo virus (isolate Rat/Peru/CLHP-2472/1997) (ALLV)

Taxonomic identifier

144752 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Arenaviridae › Arenavirus › New world arenaviruses

Virus host

Oecomys bicolor (Bicolored arboreal rice rat) [TaxID: 48011]
Oecomys roberti (Robert's arboreal rice rat) [TaxID: 48012]

Protein attributes

Sequence length	2201 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N By similarity. Interacts with protein Z; this interaction inhibits viral transcription and replication By similarity.
Subcellular location	Virion. Host cytoplasm.
Sequence similarities	Belongs to the arenaviridae RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Host cytoplasm Virion
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2201	2201	RNA-directed RNA polymerase		PRO_0000361632
Regions
	Domain	1156 – 1352	197	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q6XQI9-1 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: BBD00195D874F7D3	FASTA	2,201	252,792
	10 20 30 40 50 60 MDVHLIELRD LVRKWVPDDL ELSEQKNIML AQTQIRATVV ESLKLLSTIV EVDSCKKHSC 70 80 90 100 110 120 VHNTSKTVNA ILREHKIIGP TLPDVTPDGY CVIGDVLILL EVFVRTNQES FEKKFNQDFE 130 140 150 160 170 180 KLMQMSADLK KCGVTLVPTI DGRSTYYVDF IPDWVVERLR WLISRLMSSL REDGQEIEEL 190 200 210 220 230 240 EYERLISSLS SLENQSLGLE SLLAMREKGL SYKETLDKLF LEGMENKLTV DESRTRIMKM 250 260 270 280 290 300 FQIFRTLLES GYLERKYQTT DREDMLKRLR DHEFIVCSKS VEYTFDCPNC SVHLYKVLNL 310 320 330 340 350 360 LLNQGSRGAP HQCLGEYMKT LSICNKIKSM KILNTRRNTL LILDTIMLNK FLDLEKVFGH 370 380 390 400 410 420 VVVERVMIMQ SLMTVNDRLL SIDVLMEMLE KKMTRNPLWF LKVNEKLRKL CPPEVYQSIE 430 440 450 460 470 480 EYVHEVDRDH WFELKLTLHQ TWPAKPLIDY KGKMRCTCVE KDSNNKNQLS DLTEEKFQLL 490 500 510 520 530 540 LKKLSSFCLG ITNSLKTSAV AKLRVNQPDD YYGKVTCSEV FFQSLDKEHS AVLLYQKTGE 550 560 570 580 590 600 KSRAYGLAFN NVVTGQYTTE ASFYCDPKRF FLPIMSDVVL FRMCNEMLSW LDYLSDDVML 610 620 630 640 650 660 EVRTCLYRLV LSILCTPSKR VQVYIQGLRY FIMAFVNEFH CTGLLDKLKV TALTESERYC 670 680 690 700 710 720 MKLCDDLVVK VLNSVEDENM AKAFKFVLNT SYLCHLITKE TPDRLTDQIK CFEKFLEPKL 730 740 750 760 770 780 DFGSVIVNPD SSCELTAGQE EQFYQGLEKL FTDKKLESSY ANKPGVCKEV LNVCMSLFNS 790 800 810 820 830 840 GALEVKPLLN HDPITPSFTS TALDLSSNKS VVVPKLDELG EVLTEYDYSK LVSSVVVDLV 850 860 870 880 890 900 EHFKTKGKYV VSPRSLQYKI YKRLSNLVQQ RAGKGNKESE LTEEEFLEQV TAEQLEVINK 910 920 930 940 950 960 VETKVSRTLS GIKLSSDTEN AKHDDDYHLK KLWSKDIMVR IKAETSLHEV KDFNVDTLPF 970 980 990 1000 1010 1020 DLYRELVDAI YNDPAANSHY FSERIFNPCP LELLIKNLTL KAYKEEDFFE CFKYILISSN 1030 1040 1050 1060 1070 1080 FDNKVGKYDH KNRSRLGLSS AALLVKDEAR ISMRESNSES IAKRLDKSFF TNSSLRNLCF 1090 1100 1110 1120 1130 1140 YSDESPTERT SVSSNVGKLK FGLSYKEQVG GNRELYVGDL NTKLTTRLVE DYAESLTSDM 1150 1160 1170 1180 1190 1200 KYTCLNNENE FERALLDMKS VVRQSGLAVS MDHSKWGPHM SPALFSLMLR GLDFRLKDGT 1210 1220 1230 1240 1250 1260 LIDKEAVVNI LSWHIHKMVE VPFNVVEAYL KGFIKRGLGL MDRGGATRVE EFMFGYFDQG 1270 1280 1290 1300 1310 1320 IVPSHISSVI DMGQGILHNL SDLYGLITEQ FIVYALDLCY SSSFMAYTSS DDEILLSISN 1330 1340 1350 1360 1370 1380 SFKRNDGSMD MDLAIEALEF HYFLSDRLNK FVSPKTVAGT FASEFKSRFF IWSQEVPLLT 1390 1400 1410 1420 1430 1440 KFVAASLHNV KAKAPSQLAE TIDTILDQSV ANGVSIEIIG AIAPRTNALI TYSGHPFNLF 1450 1460 1470 1480 1490 1500 LCLEETDVRD WVDGSRGYRL QRSIENAFPD DVLPEIIRSA CRKIFYRIQS GTLEEDYIVT 1510 1520 1530 1540 1550 1560 TLQQSPDDCL KQMLTSCDVE KEAIDDICNY RWLNLRAHGD LRLVLRTKIM TSTRTLQKEE 1570 1580 1590 1600 1610 1620 VPSLIKSVQS KLSKNFVRGA KKILADAINK SAFQSCISSG FVGVCKSMGS KCVRDGKGGF 1630 1640 1650 1660 1670 1680 KYIKDILKEI KHHEKPDCHF CKELKGIYCS ELLENISEFS RPLFWDYFSL VLSNACELGN 1690 1700 1710 1720 1730 1740 WVFCKIEIPK SVYHLNNPNH FWPIKPSSHA ELEEKVGMNH VLYSIRRNFP VLFDEHISPY 1750 1760 1770 1780 1790 1800 LSDLNMLKLN WVQKIRFLDI CVAIDMTSEC LGIISHIIRR KREELYIVKQ SELSMSHTRV 1810 1820 1830 1840 1850 1860 SLPLERGFNI EPDEVCHNFL LQILFESMIH PVLLTTSQFK RYFWYSEVEL LPKEALHDLG 1870 1880 1890 1900 1910 1920 QFTQFIIDCK VLNSSRAMCL DDLDVGYVSS KVKRTDTYLN LSTFMTNLDW ENRHEYSSFE 1930 1940 1950 1960 1970 1980 DLILSSPSEV FLFEITFTFS HIRRSHKFRY DRSTNYILKT KLVIEKSELV NGEDGVYCVT 1990 2000 2010 2020 2030 2040 PHSIEYYVSQ SSGNHISLDG VSLLVLDPLI SGRELVNMDE LLQNQDVTFS APSQILSKIK 2050 2060 2070 2080 2090 2100 LDFKPFTKEI KNKFSYKLIG PDVDMSPLHL DKGAIKEGDR IVSQIEIQVS FKSVITAIEL 2110 2120 2130 2140 2150 2160 LDEDQRKIFV GNLFVYLTSL KSVNRALSMS ESDLRLLVEN YPSVIEYMLS GCDGWLNCGS 2170 2180 2190 2200 FSLIKSKTLQ CIMLADERGP YRIKGQNCRR LFPTEEAIEI E

References

[1]	"New insights into the evolutionary relationships between arenaviruses provided by comparative analysis of small and large segment sequences." Charrel R.N., Lemasson J.J., Garbutt M., Khelifa R., De Micco P., Feldmann H., de Lamballerie X. Virology 317:191-196(2003) [PubMed: 14698659] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Complete sequence determination and analysis of the large RNA segment of arenaviruses." Emonet S., de Lamballerie X., de Micco P., Charrel R.N. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY216502 Genomic RNA. Translation: AAP44538.2.
RefSeq	YP_001649212.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5848365.
Family and domain databases
InterPro	IPR014386. RNA-dir_pol_arenavirus. IPR007099. RNA-dir_pol_NSvirus. IPR010453. RNA_pol_arenavir. [Graphical view]
Pfam	PF06317. Arena_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000836. L_ArenaV. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_ALLVP

Accession

Primary (citable) accession number: Q6XQI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	February 26, 2008
Last modified:	November 3, 2009
This is version 26 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)