Reviewed, UniProtKB/Swiss-Prot Q6XQI4 (L_JUNIN)

Names and origin

Protein names

Recommended name:
    RNA-directed RNA polymerase
    EC=2.7.7.48
Alternative name(s):
    Protein L

Gene names

Name:

L

Organism

Junin arenavirus (JUNV)

Taxonomic identifier

11619 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Arenaviridae › Arenavirus › New world arenaviruses

Virus host

Homo sapiens (Human) [TaxID: 9606]
Bolomys [TaxID: 10080]
Akodon azarae (Azara's grass mouse) [TaxID: 29095]
Calomys laucha (Small vesper mouse) [TaxID: 56211]
Calomys musculinus (Drylands vesper mouse) [TaxID: 56212]

Protein attributes

Sequence length	2210 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N By similarity. Interacts with protein Z; this interaction inhibits viral transcription and replication By similarity.
Subcellular location	Virion. Host cytoplasm.
Sequence similarities	Belongs to the arenaviridae RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Host cytoplasm Virion
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2210	2210	RNA-directed RNA polymerase		PRO_0000361638
Regions
	Domain	1171 – 1368	198	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q6XQI4-1 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: 97D7A1324309C61B	FASTA	2,210	252,871
	10 20 30 40 50 60 MEESVNEIKN LIRKHFPERQ ELAYQRDIFL SQHHPSSLLL EGFKLLSSLV ELESCEAHAC 70 80 90 100 110 120 QINSDQKFVD VILSDHGILC PTLPKVIPDG FKLTGKTLIL LETFVRVNPD EFEKKWKSDM 130 140 150 160 170 180 SKLLNLKSDL LRAGITLVPV VDGRSSYSNR FLADWVVERV RWLLIDILKK SKFMQEINIE 190 200 210 220 230 240 EQEYQRLIHS LSNTKNQSLG LENIECLKKN SLGYDERLNE SLFVGVRGDI RESVIREELI 250 260 270 280 290 300 KLRFWFKKEI FDKQLGKFKF SQKSNLINDL VSLGSHKDSD VPSCPFCANK LMDVVYSIAS 310 320 330 340 350 360 HPIDEVNMKS QSDENSISID AVERCYLQAL SVCNKVKGLK VFNTRRNTLL FLDLVLLNLL 370 380 390 400 410 420 CDLFKKHDDA IVRLRNAGIV VGQMLMLVND RLLDILEAIK LIRKKLMTSP KWVQMCSRTL 430 440 450 460 470 480 KNSHQDLWSQ LEKLIKHPDM DSMMILAQAL VSDRPVMRYT IDRESEKICR HQPFSSLVEG 490 500 510 520 530 540 EQKKLFRILS SISLALVNSM KTSFSSRLLI NEREYSRYFG NVRLRECYVQ RFHLIKNTFG 550 560 570 580 590 600 LLFYQKTGEK SRCYSIYLSI NGVLEEQGSF YCDPKRFFLP IFSEDVLIEM CEEMTSWLDF 610 620 630 640 650 660 SHELMTMTRP TLRLLVLAVL CSPSKRNQTF LQGLRYFLMA YANQIHHVDL MSKLRVDCMS 670 680 690 700 710 720 GSEVLIQRMA VELFQTILSE GEDADLYFAR RFKYLLNVSY LCHLVTKETP DRLTDQIKCF 730 740 750 760 770 780 EKFIEPKVKF DCVVVNPPLN GSLTLEQEDI MIRGLDRFFS KEAKTSSDTQ IPGVSKEILS 790 800 810 820 830 840 FCISLFNRGR LKVTGELKSN PYRPNITSTA LDLSSNKSVV IPKLDELGNI LSVYDKEKLV 850 860 870 880 890 900 STCVSTMAER FKTKGRYNLD PDSMDYLILK NLTGLVSTGS RTRTNQEELS MMYESLTEDQ 910 920 930 940 950 960 VRAFEGIRND VQMTLAKMAN SEGSKVETTK LKSKNLSVDE RESLESLWAP FGVLREIKAE 970 980 990 1000 1010 1020 VSMHEVKDFD PDVFRSDVYK ELCDAVYLSP FKLTYFLEAP QDICPLGLLL KNLTTIAYQE 1030 1040 1050 1060 1070 1080 DEFFECFKYL LIQGHYDQKL GSYEHRSRSR LGFSSEVLKL KDEVRLSTRE SNSEAIADKL 1090 1100 1110 1120 1130 1140 DKSYFTNAAL RNLCFYSDDS PTEFTSISSN TGNLKFGLSY KEQVGSNREL YVGDLNTKLM 1150 1160 1170 1180 1190 1200 TRLVEDFSEA VGSSMRYTCL NSEKEFERAI CDMKMAVNNG DLSCSYDHSK WGPTMSPALF 1210 1220 1230 1240 1250 1260 LSFLYTLELK NPRDRTKVNL EPVMNILKWH LHKVVEVPIN VAEAYCVGKL KRSLGLMGCD 1270 1280 1290 1300 1310 1320 CTSVGEEFFH QYLQSRDQVP SHIMSVLDMG QGILHNTSDL YGLITEQFLC YALDLLYDVI 1330 1340 1350 1360 1370 1380 PVTYTSSDDQ VSLIKIPCLS DEKCQDRTEL LEMVCFHEFL SSKLNKFISP KSVIGTFVAE 1390 1400 1410 1420 1430 1440 FKSRFFVMGE ETPLLTKFVS AALHNVKCKT PTQLSETIDT ICDQCIANGV STHIVSKISI 1450 1460 1470 1480 1490 1500 RVNQLIRYSG YRETPFGAIE EQDVKDWVDG SRGYRLQRKI EAIFSDDKET MFIRNCARKV 1510 1520 1530 1540 1550 1560 FNDIKKGKIF EENLINLISR GGDEALSGFL QYAGCSEDEI RRTLDYRWVN LASFGDLRLV 1570 1580 1590 1600 1610 1620 LRTKLMTSRR VLEKEEMPTL IKTIQSRLSR NFTKGVKKIL AESINKSAFQ SSVASGFIGF 1630 1640 1650 1660 1670 1680 CKSMGSKCVR DGKGGFLYIK DIFTRIMPCL CEICEKKPKV IYCQKSLQEV NQFSKPILWD 1690 1700 1710 1720 1730 1740 YFSLVLTNAC ELGEWVFSAV KSPQAPLVLC NKNFFWAVKP KAVRQIEDQL GMNHVLHSIR 1750 1760 1770 1780 1790 1800 RNYPKLFEEH LAPFMNDLQV NRSLDSGRLK FLDVCVALDM MNENLGIISH LLKVRDNNVY 1810 1820 1830 1840 1850 1860 IVKQSDCASA HVRQSEYTNW EVGISPQQVC RNFMVQVVLS SMINPLVMST SCLKSFFWFN 1870 1880 1890 1900 1910 1920 EVLDLEDDSQ VDLAELTDFT LSIKNNKVSR AMFVEDIAMG YVVSSFDNIK VFLESVSVDN 1930 1940 1950 1960 1970 1980 ISLLPQEDMI DLHTVLRNVA CQEAVKLKLI IQVEHTRVST KFKLRRKMVY SYTIVSSLRV 1990 2000 2010 2020 2030 2040 DDVSTPELEL NVDAMSQCVS GSEGNHSLLD GALVIASLPL FTGHESFDLA GLFIDAGYAV 2050 2060 2070 2080 2090 2100 TNDDNILSHV KLNFGDFYSE LGNKYAYDLI GPNNPGEPLV LKEGIFYRGN ERLSTYKVEL 2110 2120 2130 2140 2150 2160 SGDVIVKAIG ALEDIDSVET LLCQLWPYLK TTSQTILFQQ EDFVLVYDLH KEQLVRSLDK 2170 2180 2190 2200 2210 FGDWLEFSNF KVAFSRSLND LLVSDPQGQF RLKGVTCRPL KHKVEIKDID

References

[1]	"New insights into the evolutionary relationships between arenaviruses provided by comparative analysis of small and large segment sequences." Charrel R.N., Lemasson J.J., Garbutt M., Khelifa R., De Micco P., Feldmann H., de Lamballerie X. Virology 317:191-196(2003) [PubMed: 14698659] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: MC2.
[2]	"Phylogeny of the genus Arenavirus." Charrel R.N., de Lamballerie X., Emonet S. Curr. Opin. Microbiol. 11:362-368(2008) [PubMed: 18602020] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: MC2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY216507 Genomic RNA. Translation: AAP44543.2.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR014386. RNA-dir_pol_arenavirus. IPR007099. RNA-dir_pol_NSvirus. IPR010453. RNA_pol_arenavir. [Graphical view]
Pfam	PF06317. Arena_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000836. L_ArenaV. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_JUNIN

Accession

Primary (citable) accession number: Q6XQI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	February 26, 2008
Last modified:	November 3, 2009
This is version 25 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)