Q6XQH7 (L_OLVVA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L
EC=2.7.7.48

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Gene names

Name:

L

Organism

Oliveros virus (isolate Mouse/Argentina/RIID 3229/1990) (OLVV) [Complete proteome]

Taxonomic identifier

42764 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Arenaviridae › Arenavirus › New world arenaviruses

Virus host

Bolomys [TaxID: 10080]

Protein attributes

Sequence length	2234 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N By similarity. Interacts with protein Z; this interaction inhibits viral transcription and replication By similarity.
Subcellular location	Virion. Host cytoplasm.
Sequence similarities	Belongs to the arenaviridae RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Cap snatching RNA replication
Cellular component	Host cytoplasm Virion
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2234	2234	RNA-directed RNA polymerase L		PRO_0000361643
Regions
	Domain	1184 – 1383	200	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q6XQH7 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: 4EC3FE652D19A594	FASTA	2,234	256,250
	10 20 30 40 50 60 MDESVSSLFD LLRKHFPAKE EISRQITVVT SQTEMRMILT EGFKLLSLLI ELDSCEVNNC 70 80 90 100 110 120 SHNKEDLTVE AILSKDNILT IALPRIVPDG YSLYGNVLIL LETFVRVNPS SFEQKYNQDM 130 140 150 160 170 180 NKLLSLKNDL QLCGITLVPL VDGRTNYYNK FVDDWVIERF RWLLTQIMKV AKESGESIEE 190 200 210 220 230 240 LEYQRLVTSL SKLENQSLGF ENIIKMPQTG IDYRDKLKAR MFANLSNKMK ESEINQSLLS 250 260 270 280 290 300 LKLAFDEAYN DESHLKKFQK TNKEDLIFKL GQQINLSDEK LSCMSCSSKL FSIVSSITQN 310 320 330 340 350 360 RDKLDSHVMS VSNAKLWHHE SGIANVNEYL RILSVCNKIK SAKILNTRRN TLLFLDMIVL 370 380 390 400 410 420 NFIDDCWKND PTILFQFKKS GLLVGQLAYF VNDRFFDLLL LKELLSKKLK SSPDWIHRCL 430 440 450 460 470 480 CNIRKQEFFD ISGVEFWIRQ PDYESVEELC CALEPVKPKL QYCRDEDNHE NHKLDLADKD 490 500 510 520 530 540 NYFTCLSVLS SVCLGLVNSM KTSFTSKMVI NEKSPNNFYG EVELKECYCQ RFYVSDEITG 550 560 570 580 590 600 LLFYQKTGEK SRCYSIGVTM HGSYKYIGSF YCDPKRFFLP IFSQVVLFQM TEEMMSWLPE 610 620 630 640 650 660 EPSYKEPVVA NLRKLILMLL CNPSKRNQNF LQGMRYFIMA YVNQFHSVEL MSKLEVPCKS 670 680 690 700 710 720 VSEECVQKLT YNLLVDVLTK GDVNEHMTRK FKFCLNVSYL CHLITKETPD RLTDQIKCFE 730 740 750 760 770 780 KFLEPKLKFK SVIINPNLTG DLTEEQEEQL LNSIEKLLGK GLQDINDSSE PGISRELLSM 790 800 810 820 830 840 CISAFNRDLL RVNGKLKNDP YKPNFTSTAL DLSSNKSVVI PKLDELGNPI SKYDYELLVS 850 860 870 880 890 900 SCIASMAESF KTKGKYKLDP TSQEFLILKN LYSLMSKSKR DDHMKDSEDS KQNLSSDLEN 910 920 930 940 950 960 LSEEQVLILE QVKRDVNLAL SKMRETKLKE KTEARQSSSG SSLKNQQKRQ AELQERLSEL 970 980 990 1000 1010 1020 WSEFMCMKII TVEVSLHEIK DFDPDLIDHT TLKSMLDKLY NSDLASEFFL EEILNPCPLE 1030 1040 1050 1060 1070 1080 FLVKNMTTSS YLEGDLFECF KYTLISAGFD QKLGTYEHKN KTRLGFKYEA LKVREEGRMS 1090 1100 1110 1120 1130 1140 LRESNSEAIA RRLDRSVFSN SALRNLCFYS DESPISYSHV SSDTGKLKFG LSYKEQVGSN 1150 1160 1170 1180 1190 1200 RELYVGDLNT KLMTRLIEDF SESVVSNMNY SCLNSEKEFE RSVMEMKMSV NLGEMNFSLD 1210 1220 1230 1240 1250 1260 HSKWGPYMSP VIFAAFLQGL KLEQGSMCTP VSVEPIITLL SWHIHKVVEV PYNVIHAYMT 1270 1280 1290 1300 1310 1320 GMIKRQLGLM SPGESSKTEA FIHRLLVDER EPLSHVMSVL DMGQGILHNT SDLYGLVTEQ 1330 1340 1350 1360 1370 1380 FINYAMRILY DVSMTSFTSS DDQITMVKLN EDLKDMDNPE VISNWERMIN FHTFISSKFN 1390 1400 1410 1420 1430 1440 KFVSPKTVIG TFAAEFKSRF FVWGEEVPLL TKFVSAALHN IKCKTPIQLS ETIDTISDQC 1450 1460 1470 1480 1490 1500 VANGVSVEIV SCISNRTNKL VRYSGFPDNP FLSVENMDVK DWVDGNRGYR LQRNIESHLE 1510 1520 1530 1540 1550 1560 VDGCTRFVRQ AARKVFRNIK SGKIMEQTLV NLVQEDGDKA FQGFMKSVDV SDDDIKLLQN 1570 1580 1590 1600 1610 1620 FRWINLSTHG DMRLVLRTKL MSSRRIIEQE EIPGLIKSIQ SKLSKNFVRG AKRILADSIN 1630 1640 1650 1660 1670 1680 KSAFQSSIAS GFIGFCKSMG SKCVRLGGGG FGYIKDIKNK VKHDCLCDIC FRWRGCVYCP 1690 1700 1710 1720 1730 1740 SSCADVFEFS RPLMWDYFTL VLTNACELGE WVFEDVEIPK DLYFLRNPNL FWLVKPRVTC 1750 1760 1770 1780 1790 1800 QLEERLGLSH ILQSIRKNYP TLFETHLSPF MSDFMVGKTL GSLTVKFLDL CVALDLANEN 1810 1820 1830 1840 1850 1860 LGITKHFLKE RRHEIYVVKQ DESSQSHIRN VKGIESSVEL NSMQVCNNFL TQLLMSSFIQ 1870 1880 1890 1900 1910 1920 PLVLTSSVFK KFNWFAEVLC LKTEEEVCLK QLTDFVLQVK KFNVDRAMHI EDLSAGYISS 1930 1940 1950 1960 1970 1980 TINVTSFSLS VPTFLECVDS DFINKEGNEP GDFKDLLSSE FTKDTLTLDF CIQVSHIKRS 1990 2000 2010 2020 2030 2040 VKFNVKRTLV YTLAVRTQIE KKIILEAIGT DDQISLIVSE LDLFCSGHTG NHFVLDAAPL 2050 2060 2070 2080 2090 2100 IYSEPLIAGS LKFDLLSMLR DQELSLTSSE KMPTFNFDFS SQKHHIVNKF AYKLVGPSVY 2110 2120 2130 2140 2150 2160 DEPLVLNKGI VYSGERKLTS LGVDVSGERI VQAMGELDSI SEQELFLTNL WGYSKETDIK 2170 2180 2190 2200 2210 2220 VRIIQDNLKI LTDNYFVQLK NSLKTFAEWL NLGNYMLCYS KTLDTIMISD VSGRIKLKGV 2230 ICRKLIEDEV MEVE

References

[1]	"New insights into the evolutionary relationships between arenaviruses provided by comparative analysis of small and large segment sequences." Charrel R.N., Lemasson J.J., Garbutt M., Khelifa R., De Micco P., Feldmann H., de Lamballerie X. Virology 317:191-196(2003) [PubMed: 14698659] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Complete sequence determination and analysis of the large RNA segment of arenaviruses." Emonet S., de Lamballerie X., de Micco P., Charrel R.N. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY216514 Genomic RNA. Translation: AAP44550.2.
RefSeq	YP_001649214.1. NC_010250.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5848321.
Family and domain databases
InterPro	IPR014386. RNA-dir_pol_arenavirus. IPR007099. RNA-dir_pol_NSvirus. IPR010453. RNA_pol_arenavir. [Graphical view]
Pfam	PF06317. Arena_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000836. L_ArenaV. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_OLVVA

Accession

Primary (citable) accession number: Q6XQH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	February 26, 2008
Last modified:	May 31, 2011
This is version 32 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program