Reviewed, UniProtKB/Swiss-Prot Q6XQH2 (L_CPXVB)

Names and origin

Protein names

Recommended name:
    RNA-directed RNA polymerase
    EC=2.7.7.48
Alternative name(s):
    Protein L

Gene names

Name:

L

Organism

Cupixi virus (isolate Rat/Brasil/BeAn 119303/1970) (CPXV)

Taxonomic identifier

208899 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Arenaviridae › Arenavirus › New world arenaviruses

Virus host

Oryzomys megacephalus (Large-headed rice rat) (Oryzomys capito) [TaxID: 89099]

Protein attributes

Sequence length	2208 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N By similarity. Interacts with protein Z; this interaction inhibits viral transcription and replication By similarity.
Subcellular location	Virion. Host cytoplasm.
Sequence similarities	Belongs to the arenaviridae RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Host cytoplasm Virion
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2208	2208	RNA-directed RNA polymerase		PRO_0000361635
Regions
	Domain	1172 – 1370	199	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q6XQH2-1 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: 637E5763AF088DFD	FASTA	2,208	250,981
	10 20 30 40 50 60 MDEKISSLKD FVRKQIPDRP EFFYQKEALL SQVEVSLILT EGFKLMSCLV EVESCEKNSC 70 80 90 100 110 120 VHNSEQKFVD TILAENGIVG PTLPKVMPDG YRYFNKTLLL LEVFVRVRPD EFEKKWKSDM 130 140 150 160 170 180 GKLLSLKDDL LRCGISLVPI VDGRCSYNTS IIPEWATERF RWLLIELLKE SKEAMDFEIE 190 200 210 220 230 240 DQEYQRLIHS LSRTCNQSLG FENIEQLKKV HLNYEDRLNE VILAGLNSDL KESVIREELI 250 260 270 280 290 300 KLKAWYKKEV FEKGHGNFVR TNQTSLLKTL QEIGSHAGTT VPECPMCCSK VFDLCYQMML 310 320 330 340 350 360 KIEGKESLNS SVSSDNNNPQ ISLVGREYLY VLSVCNKIKG KKIFNTRRNT LLFLDLIILN 370 380 390 400 410 420 FVTEVFKKVP LGIQSLKVEG LIIGQMLLLT NDRALDILSA RRLLIKKIEC NESWVKKCGD 430 440 450 460 470 480 TLRRVEPSFW TSVCNYVKLP DFESLLLLAE VLCSDAPLLR YEPVQVEESH CTHKDFQLLN 490 500 510 520 530 540 INQQDCLFEC LSHISLSLIN SMKTSFSSRL LINEKDYKRY FGTVRLKECY VQRFLFTEGK 550 560 570 580 590 600 CGFLFYQKTG ERSRCFSIYL SENGQLTELG SFYADPKRYF LPIFSGCVLR SMCSEMITWL 610 620 630 640 650 660 DFDEELMHVV KPQLRSLVLS ILCSPTKRAQ NFLQGLRYFI MAFVNQAHHK QLMSKLIVEC 670 680 690 700 710 720 KSASDVLIQR LATKVFYTIL TFGEDPGIHM TRKFKFLLNV SYLCHLITKE TPDRLTDQIK 730 740 750 760 770 780 CFEKFLEPKL EFGSVVVNPS LNGILDPKQE DNMLNGLEKL FSKSLYDVED LKRPGISKDV 790 800 810 820 830 840 LNYCLSLFTR GKLKVTGVLK TDPFKPSFTS TALDLSSNKS VVVPKLDELG NIVSVYDKQK 850 860 870 880 890 900 LISSCVASMA ERFRTKGRFN LDPDTIDFLI MKNLTNLLSI SGESPKASEE LSLLYENLPE 910 920 930 940 950 960 EITQVYDEIK NDIQVTLGKI GAKGSYKQKN GKEGKAGSPS NAETLESIWA PFGVLREIKI 970 980 990 1000 1010 1020 ETSTHEIKDF DPGLLSMDIY EKLCTTVFES SLKSSFFIDE VLSICPLELL LKNLTTKSFK 1030 1040 1050 1060 1070 1080 ENDFFECFKY ILIQAGYDQR LGTYEHRSRA RFGFREEVVR LRDDVRVSDR ESNSEAIAKR 1090 1100 1110 1120 1130 1140 LDRSFFTSAA LRNLCFYSEE SPTEYTCVSS NTGNMKFGLS YKEQVGSNRE LYVGDLNTKM 1150 1160 1170 1180 1190 1200 MTRLVEDFAE AVCNSMHYTC LNSESEFDRA ICDMKMAVNN GDLCLSMDHS KWGPFMSPAL 1210 1220 1230 1240 1250 1260 FHRFLSGAKL KTMRLGDSVD TKPVLNVLKW HIHKVVEVPY NVAEAYCTGM LKRRLGLMSL 1270 1280 1290 1300 1310 1320 QTQSISEAFF HQEIQTKKEV PSHIMSVLDM GQGILHNLSD LYGLISEQFL NYCLDFLYDA 1330 1340 1350 1360 1370 1380 IPTSYTSSDD QITMIRMPQQ SMETAEEGNA DWLELLCFHD FLSSKLNKFV SPKTVCGTFA 1390 1400 1410 1420 1430 1440 AEFKSRFFVM GEETPLLTKF VSAALHNVKC KTPSQLAETI DTICDQCVAN GVGVSIVSEI 1450 1460 1470 1480 1490 1500 SKRVNRLIKY SGYPLNPFLA VENQDVKDWV DGSRGYRLQR NIESCSIQES VLKQVRKFAK 1510 1520 1530 1540 1550 1560 DVFLKIKRGQ VFEEHLIQLI GADGDSAMEG FLSYVDCDKD KLREILEHRW LNLSANGDLR 1570 1580 1590 1600 1610 1620 LVLRTKLMSS KRVLEKEQIP TLVKTLQSKL SKSFTKGAKK ILAESINKSA FQASVASGFI 1630 1640 1650 1660 1670 1680 GFCESVGSKC VRDGSGGFLY IREVVSKIIS CQCTLCSANP GIIFCKNALS NVSNFSRPIL 1690 1700 1710 1720 1730 1740 WDYFSLVLTN ACELGEWVFS PTKRPQQPNL LQNPNLFWAV KPKSVRLIED QLGLGHVLQS 1750 1760 1770 1780 1790 1800 IRRSYPKIFE EHLIPFMSDL QVNRTIDFTR LKYLDVCVAI DMMNENLGIV SHLLKRKDNS 1810 1820 1830 1840 1850 1860 VYIVKQNECS VAHVREVQYV DHDIGLSPQQ VCTNFKLQLV LSSMISPLVI STSVLKSFFW 1870 1880 1890 1900 1910 1920 YNEVLKLEDN SLIDIGELTD FVILNKSYGV ERVMYLEDMA MGYVVSSVDE PEIHLINAWV 1930 1940 1950 1960 1970 1980 LTEHDVKKLE SGSKVGDKDG KALAITLNIQ FRHRRHSTKY HFTKGVVYSF TVEFQVPPSL 1990 2000 2010 2020 2030 2040 QGPNLNTVPV REMVLNASGM LGDHQSLDGV PLVASHPLMT GKKPIDLLTL LSESDIQISD 2050 2060 2070 2080 2090 2100 DTGQLMAVYL DFSEFQSLIE DKYSFKIVGP ERLDTPIILK GGVYMSEGKR LSTLMVELSG 2110 2120 2130 2140 2150 2160 NVIVKALGAI ETDKEVGSLL LGLWPYIKTT GQKIKMDQND FLLLYESHRE LLLKSLEGLG 2170 2180 2190 2200 GWLDFLDFSV CFSKSLSDLV ISDNTGSLRL KGVTCRPIHN PRIVEDID

References

[1]	"New insights into the evolutionary relationships between arenaviruses provided by comparative analysis of small and large segment sequences." Charrel R.N., Lemasson J.J., Garbutt M., Khelifa R., De Micco P., Feldmann H., de Lamballerie X. Virology 317:191-196(2003) [PubMed: 14698659] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Complete sequence determination and analysis of the large RNA segment of arenaviruses." Emonet S., de Lamballerie X., de Micco P., Charrel R.N. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY216519 Genomic RNA. Translation: AAP44555.2.
RefSeq	YP_001649218.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5848386.
Family and domain databases
InterPro	IPR014386. RNA-dir_pol_arenavirus. IPR007099. RNA-dir_pol_NSvirus. IPR010453. RNA_pol_arenavir. [Graphical view]
Pfam	PF06317. Arena_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000836. L_ArenaV. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_CPXVB

Accession

Primary (citable) accession number: Q6XQH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	February 26, 2008
Last modified:	January 19, 2010
This is version 30 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)